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Protein

Afadin- and alpha-actinin-binding protein

Gene

SSX2IP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed:23816619, PubMed:26545777). Involved in ciliogenesis (PubMed:24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed:24356449).By similarity1 Publication2 Publications

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • centrosome organization Source: UniProtKB
  • cilium assembly Source: UniProtKB
  • intraciliary transport involved in cilium morphogenesis Source: UniProtKB
  • regulation of cell motility Source: Ensembl
  • regulation of Rac protein signal transduction Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Afadin- and alpha-actinin-binding protein
Short name:
ADIP
Alternative name(s):
Afadin DIL domain-interacting protein
SSX2-interacting protein
Gene namesi
Name:SSX2IP
Synonyms:KIAA0923
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:16509. SSX2IP.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: Ensembl
  • cell leading edge Source: Ensembl
  • centriolar satellite Source: UniProtKB
  • ciliary basal body Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • protein complex Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38155.

Polymorphism and mutation databases

BioMutaiSSX2IP.
DMDMi54035738.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Afadin- and alpha-actinin-binding proteinPRO_0000064455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei290 – 2901PhosphoserineCombined sources
Modified residuei293 – 2931PhosphoserineCombined sources
Modified residuei312 – 3121PhosphoserineCombined sources
Modified residuei536 – 5361PhosphoserineCombined sources
Modified residuei540 – 5401PhosphoserineCombined sources
Modified residuei542 – 5421PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y2D8.
MaxQBiQ9Y2D8.
PaxDbiQ9Y2D8.
PeptideAtlasiQ9Y2D8.
PRIDEiQ9Y2D8.

PTM databases

iPTMnetiQ9Y2D8.
PhosphoSiteiQ9Y2D8.

Expressioni

Tissue specificityi

Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.

Developmental stagei

Expressed in interphase and M phase cells. Down-regulated by the miRNA miR338-3p.2 Publications

Gene expression databases

BgeeiENSG00000117155.
CleanExiHS_SSX2IP.
ExpressionAtlasiQ9Y2D8. baseline and differential.
GenevisibleiQ9Y2D8. HS.

Organism-specific databases

HPAiHPA027306.

Interactioni

Subunit structurei

Interacts with afadin and alpha-actinin (By similarity). Interacts with VAV2 (By similarity). Interacts with SSX2 and SSX3. Does not interact with SSX1 and SSX4 (PubMed:12007189). Interacts with PCM1 (By similarity). Interacts with WRAP73 (PubMed:26545777).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
A8K9323EBI-2212028,EBI-10174671
ABLIM3O949293EBI-2212028,EBI-351267
AENQ8WTP83EBI-2212028,EBI-8637627
AP1M1Q9BXS53EBI-2212028,EBI-541426
BEX2Q9BXY83EBI-2212028,EBI-745073
BYSLQ138955EBI-2212028,EBI-358049
C11orf54Q9H0W93EBI-2212028,EBI-740204
C14orf105Q17R993EBI-2212028,EBI-10238351
C20orf195Q9BVV23EBI-2212028,EBI-744935
CARD9Q9H2573EBI-2212028,EBI-751319
CCHCR1Q8TD31-33EBI-2212028,EBI-10175300
CCNHP519464EBI-2212028,EBI-741406
CDC23Q9UJX23EBI-2212028,EBI-396137
CDCA7LQ96GN53EBI-2212028,EBI-5278764
CEP55D3DR373EBI-2212028,EBI-10173536
CHCHD3Q9NX633EBI-2212028,EBI-743375
DAXXQ9UER73EBI-2212028,EBI-77321
DRG1Q9Y2953EBI-2212028,EBI-719554
EHHADHQ084263EBI-2212028,EBI-2339219
EIF4A2Q142403EBI-2212028,EBI-73473
EIF4A2Q14240-23EBI-2212028,EBI-10232522
FAM124BQ9H5Z63EBI-2212028,EBI-741626
FAM161AQ3B8203EBI-2212028,EBI-719941
FAM214AQ32MH53EBI-2212028,EBI-2866142
FAM64AQ9BSJ63EBI-2212028,EBI-2568609
FANCLQ9NW383EBI-2212028,EBI-2339898
FBF1Q8TES7-63EBI-2212028,EBI-10244131
FRMD6Q96NE93EBI-2212028,EBI-741729
GEMP550403EBI-2212028,EBI-744104
GOLGA8GQ08AF83EBI-2212028,EBI-10181260
INO80BQ9C0863EBI-2212028,EBI-715611
KAT5Q929933EBI-2212028,EBI-399080
KLHL42Q9P2K63EBI-2212028,EBI-739890
KRT15P190123EBI-2212028,EBI-739566
KRT31Q153233EBI-2212028,EBI-948001
KRT40Q6A1623EBI-2212028,EBI-10171697
KRTAP10-7P604093EBI-2212028,EBI-10172290
LATS1Q6PJG33EBI-2212028,EBI-10253976
LMO2P257913EBI-2212028,EBI-739696
MFAP1P550813EBI-2212028,EBI-1048159
MORN4Q8NDC43EBI-2212028,EBI-10269566
MOSP005403EBI-2212028,EBI-1757866
MRPL53Q96EL33EBI-2212028,EBI-2513715
NAA10P412273EBI-2212028,EBI-747693
NOL12Q9UGY13EBI-2212028,EBI-716098
PCM1Q1515410EBI-2212028,EBI-741421
PHC2Q8IXK03EBI-2212028,EBI-713786
PKN1Q165123EBI-2212028,EBI-602382
POLLQ9UGP5-23EBI-2212028,EBI-10320765
PRKAA1Q131313EBI-2212028,EBI-1181405
PRPF31F1T0A53EBI-2212028,EBI-10177194
PSMA1P257863EBI-2212028,EBI-359352
RYDENQ9NUL53EBI-2212028,EBI-10313866
SCNM1Q9BWG63EBI-2212028,EBI-748391
SSX2BQ163858EBI-2212028,EBI-2210673
SSX3Q999093EBI-2212028,EBI-10295431
SYT17Q9BSW73EBI-2212028,EBI-745392
taxP034103EBI-2212028,EBI-9676218From a different organism.
TBPQ32MN63EBI-2212028,EBI-10239991
TEAD4D3DUQ63EBI-2212028,EBI-10176734
TFIP11Q9UBB93EBI-2212028,EBI-1105213
TOP3BO959853EBI-2212028,EBI-373403
TP53BP2Q13625-33EBI-2212028,EBI-10175039
TRIM37O949724EBI-2212028,EBI-741602
TRIM42A1L4B63EBI-2212028,EBI-10172216
TRIM54Q9BYV23EBI-2212028,EBI-2130429
TTC23Q5W5X93EBI-2212028,EBI-6447954
WDR5P619643EBI-2212028,EBI-540834
WRAP73Q9P2S59EBI-2212028,EBI-1054904
Wrap73Q9JM983EBI-2212028,EBI-11694665From a different organism.
XIAPP981703EBI-2212028,EBI-517127
ZBTB24O431673EBI-2212028,EBI-744471
ZGPATQ8N5A54EBI-2212028,EBI-3439227
ZMAT2Q96NC03EBI-2212028,EBI-2682299
ZNF124Q159733EBI-2212028,EBI-2555767
ZNF250P15622-33EBI-2212028,EBI-10177272
ZNF3P170363EBI-2212028,EBI-1640965
ZNF417Q8TAU33EBI-2212028,EBI-740727
ZNF587Q96SQ53EBI-2212028,EBI-6427977
ZNF792Q3KQV33EBI-2212028,EBI-10240849
ZRSR2Q156963EBI-2212028,EBI-6657923
ZSCAN12O433093EBI-2212028,EBI-1210440

Protein-protein interaction databases

BioGridi125567. 236 interactions.
IntActiQ9Y2D8. 302 interactions.
MINTiMINT-3080846.
STRINGi9606.ENSP00000340279.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2D8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili131 – 22797Sequence analysisAdd
BLAST
Coiled coili266 – 29328Sequence analysisAdd
BLAST
Coiled coili374 – 46087Sequence analysisAdd
BLAST

Domaini

Both the N-terminal (up to position 79) and the C-terminal (from position 304) sequences are required for interaction with SSX2.

Sequence similaritiesi

Belongs to the ADIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IED3. Eukaryota.
ENOG410XWZ9. LUCA.
GeneTreeiENSGT00390000007688.
HOVERGENiHBG050460.
InParanoidiQ9Y2D8.
KOiK06085.
OMAiFLNMTTF.
OrthoDBiEOG091G0HRZ.
PhylomeDBiQ9Y2D8.
TreeFamiTF332889.

Family and domain databases

InterProiIPR021622. Afadin/alpha-actinin-bd.
[Graphical view]
PfamiPF11559. ADIP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2D8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDWMTVTDP GLSSESKTIS QYTSETKMSP SSLYSQQVLC SSIPLSKNVH
60 70 80 90 100
SFFSAFCTED NIEQSISYLD QELTTFGFPS LYEESKGKET KRELNIVAVL
110 120 130 140 150
NCMNELLVLQ RKNLLAQENV ETQNLKLGSD MDHLQSCYSK LKEQLETSRR
160 170 180 190 200
EMIGLQERDR QLQCKNRNLH QLLKNEKDEV QKLQNIIASR ATQYNHDMKR
210 220 230 240 250
KEREYNKLKE RLHQLVMNKK DKKIAMDILN YVGRADGKRG SWRTGKTEAR
260 270 280 290 300
NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR
310 320 330 340 350
ERVDDSTGTV ISDVEEDAGE LSRESMWDLS CETVREQLTN SIRKQWRILK
360 370 380 390 400
SHVEKLDNQV SKVHLEGFND EDVISRQDHE QETEKLELEI QQCKEMIKTQ
410 420 430 440 450
QQLLQQQLAT AYDDDTTSLL RDCYLLEEKE RLKEEWSLFK EQKKNFERER
460 470 480 490 500
RSFTEAAIRL GLERKAFEEE RASWLKQQFL NMTTFDHQNS ENVKLFSAFS
510 520 530 540 550
GSSDWDNLIV HSRQPQKKPH SVSNGSPVCM SKLTKSLPAS PSTSDFCQTR
560 570 580 590 600
SCISEHSSIN VLNITAEEIK PNQVGGECTN QKWSVASRPG SQEGCYSGCS
610
LSYTNSHVEK DDLP
Length:614
Mass (Da):71,236
Last modified:October 11, 2004 - v3
Checksum:i427903EF86A6FE31
GO
Isoform 2 (identifier: Q9Y2D8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     557-560: SSIN → RAKN
     561-614: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):65,494
Checksum:i6801860E2CDEFDF5
GO
Isoform 3 (identifier: Q9Y2D8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Note: No experimental confirmation available.
Show »
Length:587
Mass (Da):68,274
Checksum:i2E42AE655DD8EF27
GO

Sequence cautioni

The sequence BAA76767 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321V → A in AAQ72373 (Ref. 2) Curated
Sequence conflicti405 – 4051Missing in AAH64389 (PubMed:15489334).Curated
Sequence conflicti576 – 5761G → V in AAQ72373 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti578 – 5781C → R.2 Publications
Corresponds to variant rs1057746 [ dbSNP | Ensembl ].
VAR_056726

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2727Missing in isoform 3. 1 PublicationVSP_046368Add
BLAST
Alternative sequencei557 – 5604SSIN → RAKN in isoform 2. 1 PublicationVSP_011724
Alternative sequencei561 – 61454Missing in isoform 2. 1 PublicationVSP_011725Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY367055 mRNA. Translation: AAQ72373.1.
AB023140 mRNA. Translation: BAA76767.2. Different initiation.
AK292475 mRNA. Translation: BAF85164.1.
AK294057 mRNA. Translation: BAG57404.1.
AL133046 mRNA. Translation: CAB61373.1.
AC114482 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73224.1.
CH471097 Genomic DNA. Translation: EAW73226.1.
CH471097 Genomic DNA. Translation: EAW73227.1.
CH471097 Genomic DNA. Translation: EAW73228.1.
BC033637 mRNA. Translation: AAH33637.1.
BC064389 mRNA. Translation: AAH64389.1.
CCDSiCCDS53337.1. [Q9Y2D8-3]
CCDS699.1. [Q9Y2D8-1]
PIRiT42649.
RefSeqiNP_001159765.1. NM_001166293.1. [Q9Y2D8-1]
NP_001159766.1. NM_001166294.1. [Q9Y2D8-3]
NP_001159767.1. NM_001166295.1. [Q9Y2D8-3]
NP_001159889.1. NM_001166417.1. [Q9Y2D8-1]
NP_054740.3. NM_014021.3. [Q9Y2D8-1]
XP_005270484.1. XM_005270427.1. [Q9Y2D8-1]
XP_005270485.1. XM_005270428.1. [Q9Y2D8-1]
XP_006710394.1. XM_006710331.1. [Q9Y2D8-3]
XP_011538912.1. XM_011540610.1. [Q9Y2D8-1]
UniGeneiHs.22587.

Genome annotation databases

EnsembliENST00000342203; ENSP00000340279; ENSG00000117155. [Q9Y2D8-1]
ENST00000437941; ENSP00000412781; ENSG00000117155. [Q9Y2D8-3]
ENST00000481102; ENSP00000432261; ENSG00000117155. [Q9Y2D8-2]
ENST00000605755; ENSP00000474480; ENSG00000117155. [Q9Y2D8-3]
GeneIDi117178.
KEGGihsa:117178.
UCSCiuc001dkj.4. human. [Q9Y2D8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY367055 mRNA. Translation: AAQ72373.1.
AB023140 mRNA. Translation: BAA76767.2. Different initiation.
AK292475 mRNA. Translation: BAF85164.1.
AK294057 mRNA. Translation: BAG57404.1.
AL133046 mRNA. Translation: CAB61373.1.
AC114482 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73224.1.
CH471097 Genomic DNA. Translation: EAW73226.1.
CH471097 Genomic DNA. Translation: EAW73227.1.
CH471097 Genomic DNA. Translation: EAW73228.1.
BC033637 mRNA. Translation: AAH33637.1.
BC064389 mRNA. Translation: AAH64389.1.
CCDSiCCDS53337.1. [Q9Y2D8-3]
CCDS699.1. [Q9Y2D8-1]
PIRiT42649.
RefSeqiNP_001159765.1. NM_001166293.1. [Q9Y2D8-1]
NP_001159766.1. NM_001166294.1. [Q9Y2D8-3]
NP_001159767.1. NM_001166295.1. [Q9Y2D8-3]
NP_001159889.1. NM_001166417.1. [Q9Y2D8-1]
NP_054740.3. NM_014021.3. [Q9Y2D8-1]
XP_005270484.1. XM_005270427.1. [Q9Y2D8-1]
XP_005270485.1. XM_005270428.1. [Q9Y2D8-1]
XP_006710394.1. XM_006710331.1. [Q9Y2D8-3]
XP_011538912.1. XM_011540610.1. [Q9Y2D8-1]
UniGeneiHs.22587.

3D structure databases

ProteinModelPortaliQ9Y2D8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125567. 236 interactions.
IntActiQ9Y2D8. 302 interactions.
MINTiMINT-3080846.
STRINGi9606.ENSP00000340279.

PTM databases

iPTMnetiQ9Y2D8.
PhosphoSiteiQ9Y2D8.

Polymorphism and mutation databases

BioMutaiSSX2IP.
DMDMi54035738.

Proteomic databases

EPDiQ9Y2D8.
MaxQBiQ9Y2D8.
PaxDbiQ9Y2D8.
PeptideAtlasiQ9Y2D8.
PRIDEiQ9Y2D8.

Protocols and materials databases

DNASUi117178.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342203; ENSP00000340279; ENSG00000117155. [Q9Y2D8-1]
ENST00000437941; ENSP00000412781; ENSG00000117155. [Q9Y2D8-3]
ENST00000481102; ENSP00000432261; ENSG00000117155. [Q9Y2D8-2]
ENST00000605755; ENSP00000474480; ENSG00000117155. [Q9Y2D8-3]
GeneIDi117178.
KEGGihsa:117178.
UCSCiuc001dkj.4. human. [Q9Y2D8-1]

Organism-specific databases

CTDi117178.
GeneCardsiSSX2IP.
HGNCiHGNC:16509. SSX2IP.
HPAiHPA027306.
MIMi608690. gene.
neXtProtiNX_Q9Y2D8.
PharmGKBiPA38155.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IED3. Eukaryota.
ENOG410XWZ9. LUCA.
GeneTreeiENSGT00390000007688.
HOVERGENiHBG050460.
InParanoidiQ9Y2D8.
KOiK06085.
OMAiFLNMTTF.
OrthoDBiEOG091G0HRZ.
PhylomeDBiQ9Y2D8.
TreeFamiTF332889.

Miscellaneous databases

ChiTaRSiSSX2IP. human.
GeneWikiiSSX2IP.
GenomeRNAii117178.
PROiQ9Y2D8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117155.
CleanExiHS_SSX2IP.
ExpressionAtlasiQ9Y2D8. baseline and differential.
GenevisibleiQ9Y2D8. HS.

Family and domain databases

InterProiIPR021622. Afadin/alpha-actinin-bd.
[Graphical view]
PfamiPF11559. ADIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADIP_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2D8
Secondary accession number(s): A8K8W0
, B4DFE3, D3DT13, J3KR02, Q6P2P8, Q6ULS1, Q7L168, Q9UIX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Acts as an acute myeloid leukemia-associated antigen and may be used as a potential immunotherapy target for leukemia (PubMed:17686061, PubMed:19179477).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.