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Protein

Afadin- and alpha-actinin-binding protein

Gene

SSX2IP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed:23816619, PubMed:26545777). Involved in ciliogenesis (PubMed:24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed:24356449).By similarity1 Publication2 Publications

Miscellaneous

Acts as an acute myeloid leukemia-associated antigen and may be used as a potential immunotherapy target for leukemia (PubMed:17686061, PubMed:19179477).2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • centrosome cycle Source: UniProtKB
  • cilium assembly Source: UniProtKB
  • intraciliary transport involved in cilium assembly Source: UniProtKB
  • regulation of cell motility Source: Ensembl
  • regulation of Rac protein signal transduction Source: Ensembl

Keywordsi

Biological processCell adhesion, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Afadin- and alpha-actinin-binding protein
Short name:
ADIP
Alternative name(s):
Afadin DIL domain-interacting protein
SSX2-interacting protein
Gene namesi
Name:SSX2IP
Synonyms:KIAA0923
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000117155.16.
HGNCiHGNC:16509. SSX2IP.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000117155.
PharmGKBiPA38155.

Polymorphism and mutation databases

BioMutaiSSX2IP.
DMDMi54035738.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644551 – 614Afadin- and alpha-actinin-binding proteinAdd BLAST614

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei290PhosphoserineCombined sources1
Modified residuei293PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei536PhosphoserineCombined sources1
Modified residuei540PhosphoserineCombined sources1
Modified residuei542PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y2D8.
PaxDbiQ9Y2D8.
PeptideAtlasiQ9Y2D8.
PRIDEiQ9Y2D8.

PTM databases

iPTMnetiQ9Y2D8.
PhosphoSitePlusiQ9Y2D8.

Expressioni

Tissue specificityi

Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.

Developmental stagei

Expressed in interphase and M phase cells. Down-regulated by the miRNA miR338-3p.2 Publications

Gene expression databases

BgeeiENSG00000117155.
CleanExiHS_SSX2IP.
ExpressionAtlasiQ9Y2D8. baseline and differential.
GenevisibleiQ9Y2D8. HS.

Organism-specific databases

HPAiHPA027306.
HPA075946.

Interactioni

Subunit structurei

Interacts with afadin and alpha-actinin (By similarity). Interacts with VAV2 (By similarity). Interacts with SSX2 and SSX3. Does not interact with SSX1 and SSX4 (PubMed:12007189). Interacts with PCM1 (By similarity). Interacts with WRAP73 (PubMed:26545777).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
A8K9323EBI-2212028,EBI-10174671
ABLIM3O949293EBI-2212028,EBI-351267
AENQ8WTP83EBI-2212028,EBI-8637627
AP1M1Q9BXS53EBI-2212028,EBI-541426
BEX2Q9BXY83EBI-2212028,EBI-745073
BIT61P470413EBI-2212028,EBI-25889From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
BYSLQ138955EBI-2212028,EBI-358049
C11orf54Q9H0W93EBI-2212028,EBI-740204
C14orf105Q9NVL83EBI-2212028,EBI-10238351
CARD9Q9H2573EBI-2212028,EBI-751319
CCHCR1Q8TD31-33EBI-2212028,EBI-10175300
CCNHP519464EBI-2212028,EBI-741406
CDC23Q9UJX23EBI-2212028,EBI-396137
CDCA7LQ96GN53EBI-2212028,EBI-5278764
CEP55Q53EZ45EBI-2212028,EBI-747776
CHCHD3Q9NX633EBI-2212028,EBI-743375
DAXXQ9UER73EBI-2212028,EBI-77321
DRG1Q9Y2953EBI-2212028,EBI-719554
EHHADHQ084263EBI-2212028,EBI-2339219
EIF4A2Q142403EBI-2212028,EBI-73473
EIF4A2Q14240-23EBI-2212028,EBI-10232522
FAM124BQ9H5Z63EBI-2212028,EBI-741626
FAM161AQ3B8203EBI-2212028,EBI-719941
FAM214AQ32MH53EBI-2212028,EBI-2866142
FANCLQ9NW383EBI-2212028,EBI-2339898
FBF1Q8TES7-63EBI-2212028,EBI-10244131
FNDC11Q9BVV23EBI-2212028,EBI-744935
FRMD6Q96NE93EBI-2212028,EBI-741729
GCD7P325023EBI-2212028,EBI-6260From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
GEMP550403EBI-2212028,EBI-744104
GOLGA8GQ08AF83EBI-2212028,EBI-10181260
INO80BQ9C0863EBI-2212028,EBI-715611
KAT5Q929933EBI-2212028,EBI-399080
KLHL42Q9P2K63EBI-2212028,EBI-739890
KRT15P190123EBI-2212028,EBI-739566
KRT31Q153233EBI-2212028,EBI-948001
KRT40Q6A1623EBI-2212028,EBI-10171697
KRTAP10-7P604093EBI-2212028,EBI-10172290
LATS1Q6PJG33EBI-2212028,EBI-10253976
LMO2P257913EBI-2212028,EBI-739696
MFAP1P550813EBI-2212028,EBI-1048159
MORN4Q8NDC45EBI-2212028,EBI-10269566
MOSP005403EBI-2212028,EBI-1757866
MRPL53Q96EL33EBI-2212028,EBI-2513715
NAA10P412273EBI-2212028,EBI-747693
NOL12Q9UGY13EBI-2212028,EBI-716098
PCM1Q1515410EBI-2212028,EBI-741421
PHC2Q8IXK03EBI-2212028,EBI-713786
PIMREGQ9BSJ63EBI-2212028,EBI-2568609
PKN1Q165123EBI-2212028,EBI-602382
POLLQ9UGP5-23EBI-2212028,EBI-10320765
PRKAA1Q131313EBI-2212028,EBI-1181405
PRPF31Q8WWY36EBI-2212028,EBI-1567797
PSMA1P257863EBI-2212028,EBI-359352
RRN11Q047123EBI-2212028,EBI-27790From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
RYDENQ9NUL53EBI-2212028,EBI-10313866
SCNM1Q9BWG63EBI-2212028,EBI-748391
SMC5Q082043EBI-2212028,EBI-34125From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
SSX2BQ163858EBI-2212028,EBI-2210673
SSX3Q999093EBI-2212028,EBI-10295431
SYT17Q9BSW73EBI-2212028,EBI-745392
TAE1P383403EBI-2212028,EBI-21116From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
taxP034103EBI-2212028,EBI-9676218From Human T-cell leukemia virus 2.
TBPQ32MN63EBI-2212028,EBI-10239991
TEAD4D3DUQ63EBI-2212028,EBI-10176734
TFIP11Q9UBB93EBI-2212028,EBI-1105213
TOP3BO959853EBI-2212028,EBI-373403
TP53BP2Q13625-33EBI-2212028,EBI-10175039
TRIM37O949724EBI-2212028,EBI-741602
TRIM42Q8IWZ53EBI-2212028,EBI-5235829
TRIM54Q9BYV23EBI-2212028,EBI-2130429
TTC23Q5W5X93EBI-2212028,EBI-6447954
UTP25P404983EBI-2212028,EBI-25113From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
WDR5P619643EBI-2212028,EBI-540834
WRAP73Q9P2S59EBI-2212028,EBI-1054904
Wrap73Q9JM983EBI-2212028,EBI-11694665From Mus musculus.
XIAPP981703EBI-2212028,EBI-517127
YDL057WQ073793EBI-2212028,EBI-2342994From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
YLR108CQ122593EBI-2212028,EBI-30776From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
ZBTB24O431673EBI-2212028,EBI-744471
ZGPATQ8N5A54EBI-2212028,EBI-3439227
ZMAT2Q96NC03EBI-2212028,EBI-2682299
ZNF124Q159733EBI-2212028,EBI-2555767
ZNF250P15622-35EBI-2212028,EBI-10177272
ZNF3P170363EBI-2212028,EBI-1640965
ZNF417Q8TAU33EBI-2212028,EBI-740727
ZNF587Q96SQ53EBI-2212028,EBI-6427977
ZNF792Q3KQV33EBI-2212028,EBI-10240849
ZRSR2Q156963EBI-2212028,EBI-6657923
ZSCAN12O433093EBI-2212028,EBI-1210440

GO - Molecular functioni

Protein-protein interaction databases

BioGridi125567. 239 interactors.
IntActiQ9Y2D8. 320 interactors.
MINTiMINT-3080846.
STRINGi9606.ENSP00000340279.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2D8.
SMRiQ9Y2D8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili131 – 227Sequence analysisAdd BLAST97
Coiled coili266 – 293Sequence analysisAdd BLAST28
Coiled coili374 – 460Sequence analysisAdd BLAST87

Domaini

Both the N-terminal (up to position 79) and the C-terminal (from position 304) sequences are required for interaction with SSX2.

Sequence similaritiesi

Belongs to the ADIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IED3. Eukaryota.
ENOG410XWZ9. LUCA.
GeneTreeiENSGT00390000007688.
HOVERGENiHBG050460.
InParanoidiQ9Y2D8.
KOiK06085.
OMAiNDYEYRQ.
OrthoDBiEOG091G0HRZ.
PhylomeDBiQ9Y2D8.
TreeFamiTF332889.

Family and domain databases

InterProiView protein in InterPro
IPR021622. Afadin/alpha-actinin-bd.
PfamiView protein in Pfam
PF11559. ADIP. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2D8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDWMTVTDP GLSSESKTIS QYTSETKMSP SSLYSQQVLC SSIPLSKNVH
60 70 80 90 100
SFFSAFCTED NIEQSISYLD QELTTFGFPS LYEESKGKET KRELNIVAVL
110 120 130 140 150
NCMNELLVLQ RKNLLAQENV ETQNLKLGSD MDHLQSCYSK LKEQLETSRR
160 170 180 190 200
EMIGLQERDR QLQCKNRNLH QLLKNEKDEV QKLQNIIASR ATQYNHDMKR
210 220 230 240 250
KEREYNKLKE RLHQLVMNKK DKKIAMDILN YVGRADGKRG SWRTGKTEAR
260 270 280 290 300
NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR
310 320 330 340 350
ERVDDSTGTV ISDVEEDAGE LSRESMWDLS CETVREQLTN SIRKQWRILK
360 370 380 390 400
SHVEKLDNQV SKVHLEGFND EDVISRQDHE QETEKLELEI QQCKEMIKTQ
410 420 430 440 450
QQLLQQQLAT AYDDDTTSLL RDCYLLEEKE RLKEEWSLFK EQKKNFERER
460 470 480 490 500
RSFTEAAIRL GLERKAFEEE RASWLKQQFL NMTTFDHQNS ENVKLFSAFS
510 520 530 540 550
GSSDWDNLIV HSRQPQKKPH SVSNGSPVCM SKLTKSLPAS PSTSDFCQTR
560 570 580 590 600
SCISEHSSIN VLNITAEEIK PNQVGGECTN QKWSVASRPG SQEGCYSGCS
610
LSYTNSHVEK DDLP
Length:614
Mass (Da):71,236
Last modified:October 11, 2004 - v3
Checksum:i427903EF86A6FE31
GO
Isoform 2 (identifier: Q9Y2D8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     557-560: SSIN → RAKN
     561-614: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):65,494
Checksum:i6801860E2CDEFDF5
GO
Isoform 3 (identifier: Q9Y2D8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Note: No experimental confirmation available.
Show »
Length:587
Mass (Da):68,274
Checksum:i2E42AE655DD8EF27
GO

Sequence cautioni

The sequence BAA76767 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232V → A in AAQ72373 (Ref. 2) Curated1
Sequence conflicti405Missing in AAH64389 (PubMed:15489334).Curated1
Sequence conflicti576G → V in AAQ72373 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_056726578C → R2 PublicationsCorresponds to variant dbSNP:rs1057746Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0463681 – 27Missing in isoform 3. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_011724557 – 560SSIN → RAKN in isoform 2. 1 Publication4
Alternative sequenceiVSP_011725561 – 614Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY367055 mRNA. Translation: AAQ72373.1.
AB023140 mRNA. Translation: BAA76767.2. Different initiation.
AK292475 mRNA. Translation: BAF85164.1.
AK294057 mRNA. Translation: BAG57404.1.
AL133046 mRNA. Translation: CAB61373.1.
AC114482 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73224.1.
CH471097 Genomic DNA. Translation: EAW73226.1.
CH471097 Genomic DNA. Translation: EAW73227.1.
CH471097 Genomic DNA. Translation: EAW73228.1.
BC033637 mRNA. Translation: AAH33637.1.
BC064389 mRNA. Translation: AAH64389.1.
CCDSiCCDS53337.1. [Q9Y2D8-3]
CCDS699.1. [Q9Y2D8-1]
PIRiT42649.
RefSeqiNP_001159765.1. NM_001166293.1. [Q9Y2D8-1]
NP_001159766.1. NM_001166294.1. [Q9Y2D8-3]
NP_001159767.1. NM_001166295.1. [Q9Y2D8-3]
NP_001159889.1. NM_001166417.1. [Q9Y2D8-1]
NP_054740.3. NM_014021.3. [Q9Y2D8-1]
XP_005270484.1. XM_005270427.1. [Q9Y2D8-1]
XP_005270485.1. XM_005270428.1. [Q9Y2D8-1]
XP_006710394.1. XM_006710331.1. [Q9Y2D8-3]
XP_011538912.1. XM_011540610.1. [Q9Y2D8-1]
XP_016855723.1. XM_017000234.1. [Q9Y2D8-1]
UniGeneiHs.22587.

Genome annotation databases

EnsembliENST00000342203; ENSP00000340279; ENSG00000117155. [Q9Y2D8-1]
ENST00000437941; ENSP00000412781; ENSG00000117155. [Q9Y2D8-3]
ENST00000481102; ENSP00000432261; ENSG00000117155. [Q9Y2D8-2]
ENST00000605755; ENSP00000474480; ENSG00000117155. [Q9Y2D8-3]
GeneIDi117178.
KEGGihsa:117178.
UCSCiuc001dkj.4. human. [Q9Y2D8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiADIP_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2D8
Secondary accession number(s): A8K8W0
, B4DFE3, D3DT13, J3KR02, Q6P2P8, Q6ULS1, Q7L168, Q9UIX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 27, 2017
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families