Q9Y2D1 (ATF5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclic AMP-dependent transcription factor ATF-5
Short name=cAMP-dependent transcription factor ATF-5

Alternative name(s):
Activating transcription factor 5
Transcription factor ATFx

Gene names

Name:	ATF5
Synonyms:	ATFX

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Ref.8 Ref.9
Subunit structure	Binds DNA as a dimer. Interacts with PTP4A1/PRL-1 By similarity. Interacts with CCND3, but not with CCND1 or CCND2. Interacts with CDC34. Ref.8 Ref.9
Subcellular location	Cytoplasm. Nucleus Ref.9.
Post-translational modification	Ubiquitinated by CDC34 and UBE2B in order to be degraded by the proteasome. Ref.8
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: HPA
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription corepressor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DISC1	Q9NRI5	8	EBI-492509,EBI-529989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Cyclic AMP-dependent transcription factor ATF-5		PRO_0000076586
Regions
	Domain	236 – 250	15	Leucine-zipper By similarity
	DNA binding	210 – 230	21	Basic motif
	Region	119 – 217	99	Interaction with PTP4A1 By similarity
	Compositional bias	123 – 139	17	Poly-Pro
	Compositional bias	186 – 194	9	Poly-Pro
Natural variations
	Natural variant	121	1	P → L. Ref.2 Ref.7 Corresponds to variant rs283526 [ dbSNP | Ensembl ].		VAR_022786
Experimental info
	Sequence conflict	161 – 163	3	LLA → RHE in AAD28370. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2D1 [UniParc]. Last modified October 5, 2010. Version 4. Checksum: DDB2F907CA0215A0	FASTA	282	30,674
	10 20 30 40 50 60 MSLLATLGLE LDRALLPASG LGWLVDYGKL PPAPAPLAPY EVLGGALEGG LPVGGEPLAG 70 80 90 100 110 120 DGFSDWMTER VDFTALLPLE PPLPPGTLPQ PSPTPPDLEA MASLLKKELE QMEDFFLDAP 130 140 150 160 170 180 PLPPPSPPPL PPPPLPPAPS LPLSLPSFDL PQPPVLDTLD LLAIYCRNEA GQEEVGMPPL 190 200 210 220 230 240 PPPQQPPPPS PPQPSRLAPY PHPATTRGDR KQKKRDQNKS AALRYRQRKR AEGEALEGEC 250 260 270 280 QGLEARNREL KERAESVERE IQYVKDLLIE VYKARSQRTR SC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The GABAB receptor interacts directly with the related transcription factors CREB2 and ATFx." White J.H., McIllhinney R.A.J., Wise A., Ciruela F., Chan W.-Y., Emson P.C., Billinton A., Marshall F.H. Proc. Natl. Acad. Sci. U.S.A. 97:13967-13972(2000) [PubMed: 11087824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed: 15221005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-121. Tissue: Hepatoblastoma.
[3]	"cDNA clone encoding leucine-zipper protein." Kohroki J., Tanaka K. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-121. Tissue: Placenta.
[8]	"Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis." Pati D., Meistrich M.L., Plon S.E. Mol. Cell. Biol. 19:5001-5013(1999) [PubMed: 10373550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 161-282, FUNCTION, INTERACTION WITH CDC34, UBIQUITINATION.
[9]	"Cyclin D3 interacts with human activating transcription factor 5 and potentiates its transcription activity." Liu W., Sun M., Jiang J., Shen X., Sun Q., Liu W., Shen H., Gu J. Biochem. Biophys. Res. Commun. 321:954-960(2004) [PubMed: 15358120] [Abstract] Cited for: INTERACTION WITH CCND3, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF305687 mRNA. Translation: AAG22558.1. AB073613 mRNA. Translation: BAD38650.1. AB021663 mRNA. Translation: BAA78477.2. AK024402 mRNA. Translation: BAG51295.1. AC011452 Genomic DNA. No translation available. CH471177 Genomic DNA. Translation: EAW52581.1. BC005174 mRNA. Translation: AAH05174.1. AF101388 mRNA. Translation: AAD28370.1.
IPI	IPI00032057.
RefSeq	NP_001180575.1. NM_001193646.1. NP_036200.2. NM_012068.5.
UniGene	Hs.9754.
3D structure databases
ProteinModelPortal	Q9Y2D1.
SMR	Q9Y2D1. Positions 216-271.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y2D1. 5 interactions.
STRING	Q9Y2D1.
Polymorphism databases
DMDM	308153647.
Proteomic databases
PRIDE	Q9Y2D1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306139; ENSP00000307356; ENSG00000169136. ENST00000423777; ENSP00000396954; ENSG00000169136.
GeneID	22809.
KEGG	hsa:22809.
Organism-specific databases
CTD	22809.
GeneCards	GC19P050431.
H-InvDB	HIX0015352.
HGNC	HGNC:790. ATF5.
HPA	HPA030187.
MIM	606398. gene.
neXtProt	NX_Q9Y2D1.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20492.
GeneTree	ENSGT00530000063801.
HOGENOM	HBG269581.
HOVERGEN	HBG003535.
InParanoid	Q9Y2D1.
OMA	GDGFSDW.
OrthoDB	EOG4RBQKK.
PhylomeDB	Q9Y2D1.
Enzyme and pathway databases
Pathway_Interaction_DB	prlsignalingeventspathway. Signaling events mediated by PRL.
Gene expression databases
ArrayExpress	Q9Y2D1.
Bgee	Q9Y2D1.
CleanEx	HS_ATF5.
Genevestigator	Q9Y2D1.
GermOnline	ENSG00000169136. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. IPR011616. bZIP_1. [Graphical view]
KO	K09044.
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

ATF5_HUMAN

Accession

Primary (citable) accession number: Q9Y2D1
Secondary accession number(s): B3KND3, Q9BSA1, Q9UNQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 5, 2010
Last modified:	January 25, 2012
This is version 106 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.