Q9Y2C3 (B3GT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyltransferase 5
Short name=Beta-1,3-GalTase 5
Short name=Beta3Gal-T5
Short name=Beta3GalT5
Short name=b3Gal-T5
EC=2.4.1.-

Alternative name(s):
Beta-3-Gx-T5
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5

Gene names

Name:

B3GALT5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in stomach, jejunum, colon, pancreas, small intestine, testis and gastrointestinal and pancreatic cancer cell lines. Hardly detected in lung, liver, adrenal gland and peripheral blood leukocytes.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Traceable author statement. Source: ProtInc
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Beta-1,3-galactosyltransferase 5		PRO_0000219164
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 310	282	Lumenal Potential
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	27	1	S → R. Corresponds to variant rs12627708 [ dbSNP | Ensembl ].		VAR_049347
	Natural variant	85	1	M → T. Ref.3 Ref.5 Ref.7 Ref.8 Ref.9 Corresponds to variant rs3746887 [ dbSNP | Ensembl ].		VAR_020460
	Natural variant	144	1	R → H. Corresponds to variant rs734411 [ dbSNP | Ensembl ].		VAR_033536
Experimental info
	Sequence conflict	23	1	F → Y in CAB91547. Ref.3
	Sequence conflict	26	1	Y → N in CAB91547. Ref.3
	Sequence conflict	293 – 297	5	LDYWQ → WTTGR in BAA94498. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2C3 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4DD7A19E3E648AA9	FASTA	310	36,189
	10 20 30 40 50 60 MAFPKMRLMY ICLLVLGALC LYFSMYSLNP FKEQSFVYKK DGNFLKLPDT DCRQTPPFLV 70 80 90 100 110 120 LLVTSSHKQL AERMAIRQTW GKERMVKGKQ LKTFFLLGTT SSAAETKEVD QESQRHGDII 130 140 150 160 170 180 QKDFLDVYYN LTLKTMMGIE WVHRFCPQAA FVMKTDSDMF INVDYLTELL LKKNRTTRFF 190 200 210 220 230 240 TGFLKLNEFP IRQPFSKWFV SKSEYPWDRY PPFCSGTGYV FSGDVASQVY NVSKSVPYIK 250 260 270 280 290 300 LEDVFVGLCL ERLNIRLEEL HSQPTFFPGG LRFSVCLFRR IVACHFIKPR TLLDYWQALE 310 NSRGEDCPPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and characterization of a novel UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom." Isshiki S., Togayachi A., Kudo T., Nishihara S., Watanabe M., Kubota T., Kitajima M., Shiraishi N., Sasaki K., Andoh T., Narimatsu H. J. Biol. Chem. 274:12499-12507(1999) [PubMed: 10212226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon adenocarcinoma.
[2]	"Molecular cloning of a human UDP-galactose:GlcNAcbeta1,3GalNAc beta1, 3 galactosyltransferase gene encoding an O-linked core3-elongation enzyme." Zhou D., Berger E.G., Hennet T. Eur. J. Biochem. 263:571-576(1999) [PubMed: 10406968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning and expression of two beta-1,3-galactosyltransferases: beta3gal-T5 and beta3gal-T6." Amado M., Carneiro F., Clausen H. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-85.
[4]	"An endogenous retroviral long terminal repeat is the dominant promoter for human beta1,3-galactosyltransferase 5 in the colon." Dunn C.A., Medstrand P., Mager D.L. Proc. Natl. Acad. Sci. U.S.A. 100:12841-12846(2003) [PubMed: 14534330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-85. Tissue: Trachea.
[6]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-85.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-85. Tissue: Colon.
[9]	Liu Y., Saitou N. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298, VARIANT THR-85.
[10]	"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." Amado M., Almeida R., Schwientek T., Clausen H. Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,3-galactosyltransferase 5

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020337 mRNA. Translation: BAA77664.1. AF145784 Genomic DNA. Translation: AAF07880.1. AJ006078 Genomic DNA. Translation: CAB91547.1. AY372061 mRNA. Translation: AAR08910.1. AK292951 mRNA. Translation: BAF85640.1. AL163280 Genomic DNA. Translation: CAB90446.1. CH471079 Genomic DNA. Translation: EAX09629.1. CH471079 Genomic DNA. Translation: EAX09630.1. CH471079 Genomic DNA. Translation: EAX09631.1. CH471079 Genomic DNA. Translation: EAX09632.1. BC104862 mRNA. Translation: AAI04863.1. BC104864 mRNA. Translation: AAI04865.1. AB041413 Genomic DNA. Translation: BAA94498.1. AB041416 Genomic DNA. Translation: BAA94501.1.
IPI	IPI00032034.
RefSeq	NP_006048.1. NM_006057.1. NP_149360.1. NM_033170.1. NP_149361.1. NM_033171.1. NP_149362.1. NM_033172.1. NP_149363.1. NM_033173.1.
UniGene	Hs.655094.
3D structure databases
ProteinModelPortal	Q9Y2C3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y2C3.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Polymorphism databases
DMDM	13123995.
Proteomic databases
PeptideAtlas	Q9Y2C3.
PRIDE	Q9Y2C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000343118; ENSP00000343318; ENSG00000183778. ENST00000380618; ENSP00000369992; ENSG00000183778. ENST00000380620; ENSP00000369994; ENSG00000183778. ENST00000398714; ENSP00000381699; ENSG00000183778. ENST00000438430; ENSP00000403209; ENSG00000183778.
GeneID	10317.
KEGG	hsa:10317.
UCSC	uc002yyb.1. human.
Organism-specific databases
CTD	10317.
GeneCards	GC21P040928.
H-InvDB	HIX0040840.
HGNC	HGNC:920. B3GALT5.
MIM	604066. gene.
neXtProt	NX_Q9Y2C3.
PharmGKB	PA25213.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11756.
GeneTree	ENSGT00600000084320.
HOGENOM	HBG714296.
HOVERGEN	HBG094868.
InParanoid	Q9Y2C3.
OMA	YWQALEN.
OrthoDB	EOG49ZXPQ.
PhylomeDB	Q9Y2C3.
Gene expression databases
ArrayExpress	Q9Y2C3.
Bgee	Q9Y2C3.
CleanEx	HS_B3GALT5.
Genevestigator	Q9Y2C3.
GermOnline	ENSG00000183778. Homo sapiens.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
KO	K03877.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39103.
SOURCE	Search...

Entry information

Entry name

B3GT5_HUMAN

Accession

Primary (citable) accession number: Q9Y2C3
Secondary accession number(s): A8KA86 , D3DSI3, Q2M3L5, Q53Z19, Q9NY96, Q9P1X6, Q9P1X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.