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Q9Y2C3 (B3GT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,3-galactosyltransferase 5

Short name=Beta-1,3-GalTase 5
Short name=Beta3Gal-T5
Short name=Beta3GalT5
Short name=b3Gal-T5
EC=2.4.1.-
Alternative name(s):
Beta-3-Gx-T5
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5
Gene names
Name:B3GALT5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Expressed in stomach, jejunum, colon, pancreas, small intestine, testis and gastrointestinal and pancreatic cancer cell lines. Hardly detected in lung, liver, adrenal gland and peripheral blood leukocytes.

Sequence similarities

Belongs to the glycosyltransferase 31 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Beta-1,3-galactosyltransferase 5
PRO_0000219164

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 310282Lumenal Potential

Amino acid modifications

Glycosylation1301N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential

Natural variations

Natural variant271S → R.
Corresponds to variant rs12627708 [ dbSNP | Ensembl ].
VAR_049347
Natural variant851M → T. Ref.3 Ref.5 Ref.7 Ref.8 Ref.9
Corresponds to variant rs3746887 [ dbSNP | Ensembl ].
VAR_020460
Natural variant1441R → H.
Corresponds to variant rs734411 [ dbSNP | Ensembl ].
VAR_033536

Experimental info

Sequence conflict231F → Y in CAB91547. Ref.3
Sequence conflict261Y → N in CAB91547. Ref.3
Sequence conflict293 – 2975LDYWQ → WTTGR in BAA94498. Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q9Y2C3 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 4DD7A19E3E648AA9

FASTA31036,189
        10         20         30         40         50         60 
MAFPKMRLMY ICLLVLGALC LYFSMYSLNP FKEQSFVYKK DGNFLKLPDT DCRQTPPFLV 

        70         80         90        100        110        120 
LLVTSSHKQL AERMAIRQTW GKERMVKGKQ LKTFFLLGTT SSAAETKEVD QESQRHGDII 

       130        140        150        160        170        180 
QKDFLDVYYN LTLKTMMGIE WVHRFCPQAA FVMKTDSDMF INVDYLTELL LKKNRTTRFF 

       190        200        210        220        230        240 
TGFLKLNEFP IRQPFSKWFV SKSEYPWDRY PPFCSGTGYV FSGDVASQVY NVSKSVPYIK 

       250        260        270        280        290        300 
LEDVFVGLCL ERLNIRLEEL HSQPTFFPGG LRFSVCLFRR IVACHFIKPR TLLDYWQALE 

       310 
NSRGEDCPPV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and characterization of a novel UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom."
Isshiki S., Togayachi A., Kudo T., Nishihara S., Watanabe M., Kubota T., Kitajima M., Shiraishi N., Sasaki K., Andoh T., Narimatsu H.
J. Biol. Chem. 274:12499-12507(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Colon adenocarcinoma.
[2]"Molecular cloning of a human UDP-galactose:GlcNAcbeta1,3GalNAc beta1, 3 galactosyltransferase gene encoding an O-linked core3-elongation enzyme."
Zhou D., Berger E.G., Hennet T.
Eur. J. Biochem. 263:571-576(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning and expression of two beta-1,3-galactosyltransferases: beta3gal-T5 and beta3gal-T6."
Amado M., Carneiro F., Clausen H.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-85.
[4]"An endogenous retroviral long terminal repeat is the dominant promoter for human beta1,3-galactosyltransferase 5 in the colon."
Dunn C.A., Medstrand P., Mager D.L.
Proc. Natl. Acad. Sci. U.S.A. 100:12841-12846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-85.
Tissue: Trachea.
[6]"The DNA sequence of human chromosome 21."
Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. expand/collapse author list , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-85.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-85.
Tissue: Colon.
[9]Liu Y., Saitou N.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298, VARIANT THR-85.
[10]"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
Amado M., Almeida R., Schwientek T., Clausen H.
Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,3-galactosyltransferase 5

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB020337 mRNA. Translation: BAA77664.1.
AF145784 Genomic DNA. Translation: AAF07880.1.
AJ006078 Genomic DNA. Translation: CAB91547.1.
AY372061 mRNA. Translation: AAR08910.1.
AK292951 mRNA. Translation: BAF85640.1.
AL163280 Genomic DNA. Translation: CAB90446.1.
CH471079 Genomic DNA. Translation: EAX09629.1.
CH471079 Genomic DNA. Translation: EAX09630.1.
CH471079 Genomic DNA. Translation: EAX09631.1.
CH471079 Genomic DNA. Translation: EAX09632.1.
BC104862 mRNA. Translation: AAI04863.1.
BC104864 mRNA. Translation: AAI04865.1.
AB041413 Genomic DNA. Translation: BAA94498.1.
AB041416 Genomic DNA. Translation: BAA94501.1.
CCDSCCDS13667.1.
RefSeqNP_001265579.1. NM_001278650.1.
NP_006048.1. NM_006057.2.
NP_149360.1. NM_033170.2.
NP_149361.1. NM_033171.2.
NP_149362.2. NM_033172.2.
XP_005260969.1. XM_005260912.1.
UniGeneHs.733638.

3D structure databases

ProteinModelPortalQ9Y2C3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000343318.

Chemistry

ChEMBLCHEMBL2321635.

Protein family/group databases

CAZyGT31. Glycosyltransferase Family 31.

Polymorphism databases

DMDM13123995.

Proteomic databases

PaxDbQ9Y2C3.
PeptideAtlasQ9Y2C3.
PRIDEQ9Y2C3.

Protocols and materials databases

DNASU10317.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000343118; ENSP00000343318; ENSG00000183778.
ENST00000380618; ENSP00000369992; ENSG00000183778.
ENST00000380620; ENSP00000369994; ENSG00000183778.
ENST00000398714; ENSP00000381699; ENSG00000183778.
GeneID10317.
KEGGhsa:10317.
UCSCuc002yyb.1. human.

Organism-specific databases

CTD10317.
GeneCardsGC21P040928.
HGNCHGNC:920. B3GALT5.
HPAHPA054092.
HPA054684.
MIM604066. gene.
neXtProtNX_Q9Y2C3.
PharmGKBPA25213.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293774.
HOVERGENHBG094868.
InParanoidQ9Y2C3.
KOK03877.
OMARFCPQAA.
OrthoDBEOG7M3J0F.
PhylomeDBQ9Y2C3.
TreeFamTF318639.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ9Y2C3.
CleanExHS_B3GALT5.
GenevestigatorQ9Y2C3.

Family and domain databases

InterProIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERPTHR11214. PTHR11214. 1 hit.
PfamPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiB3GALT5.
GenomeRNAi10317.
NextBio39103.
PROQ9Y2C3.
SOURCESearch...

Entry information

Entry nameB3GT5_HUMAN
AccessionPrimary (citable) accession number: Q9Y2C3
Secondary accession number(s): A8KA86 expand/collapse secondary AC list , D3DSI3, Q2M3L5, Q53Z19, Q9NY96, Q9P1X6, Q9P1X7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM