Reviewed, UniProtKB/Swiss-Prot Q9Y2C3 (B3GT5_HUMAN)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyltransferase 5
      Short name=Beta-1,3-GalTase 5
      Short name=Beta3Gal-T5
      Short name=b3Gal-T5
    EC=2.4.1.-
Alternative name(s):
    Beta-3-Gx-T5
    UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5
    UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5

Gene names

Name:

B3GALT5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in stomach, jejunum, colon, pancreas, small intestine, testis and gastrointestinal and pancreatic cancer cell lines. Hardly detected in lung, liver, adrenal gland and peripheral blood leukocytes.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Beta-1,3-galactosyltransferase 5		PRO_0000219164
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 310	282	Lumenal Potential
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	27	1	S → R: dbSNP rs12627708.		VAR_049347
	Natural variant	85	1	M → T: dbSNP rs3746887. Ref.3 Ref.6		VAR_020460
	Natural variant	144	1	R → H: dbSNP rs734411.		VAR_033536
Experimental info
	Sequence conflict	23	1	F → Y in CAB91547. Ref.3
	Sequence conflict	26	1	Y → N in CAB91547. Ref.3
	Sequence conflict	293 – 297	5	LDYWQ → WTTGR in BAA94498. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2C3-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4DD7A19E3E648AA9	FASTA	310	36,189
	10 20 30 40 50 60 MAFPKMRLMY ICLLVLGALC LYFSMYSLNP FKEQSFVYKK DGNFLKLPDT DCRQTPPFLV 70 80 90 100 110 120 LLVTSSHKQL AERMAIRQTW GKERMVKGKQ LKTFFLLGTT SSAAETKEVD QESQRHGDII 130 140 150 160 170 180 QKDFLDVYYN LTLKTMMGIE WVHRFCPQAA FVMKTDSDMF INVDYLTELL LKKNRTTRFF 190 200 210 220 230 240 TGFLKLNEFP IRQPFSKWFV SKSEYPWDRY PPFCSGTGYV FSGDVASQVY NVSKSVPYIK 250 260 270 280 290 300 LEDVFVGLCL ERLNIRLEEL HSQPTFFPGG LRFSVCLFRR IVACHFIKPR TLLDYWQALE 310 NSRGEDCPPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and characterization of a novel UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom." Isshiki S., Togayachi A., Kudo T., Nishihara S., Watanabe M., Kubota T., Kitajima M., Shiraishi N., Sasaki K., Andoh T., Narimatsu H. J. Biol. Chem. 274:12499-12507(1999) [PubMed: 10212226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon adenocarcinoma.
[2]	"Molecular cloning of a human UDP-galactose:GlcNAcbeta1,3GalNAc beta1, 3 galactosyltransferase gene encoding an O-linked core3-elongation enzyme." Zhou D., Berger E.G., Hennet T. Eur. J. Biochem. 263:571-576(1999) [PubMed: 10406968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning and expression of two beta-1,3-galactosyltransferases: beta3gal-T5 and beta3gal-T6." Amado M., Carneiro F., Clausen H. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-85.
[4]	"An endogenous retroviral long terminal repeat is the dominant promoter for human beta1,3-galactosyltransferase 5 in the colon." Dunn C.A., Medstrand P., Mager D.L. Proc. Natl. Acad. Sci. U.S.A. 100:12841-12846(2003) [PubMed: 14534330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-85. Tissue: Colon.
[7]	Liu Y., Saitou N. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298, VARIANT THR-85.
[8]	"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." Amado M., Almeida R., Schwientek T., Clausen H. Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,3-galactosyltransferase 5

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB020337 mRNA. Translation: BAA77664.1. AF145784 Genomic DNA. Translation: AAF07880.1. AJ006078 Genomic DNA. Translation: CAB91547.1. AY372061 mRNA. Translation: AAR08910.1. AL163280 Genomic DNA. Translation: CAB90446.1. BC104862 mRNA. Translation: AAI04863.1. BC104864 mRNA. Translation: AAI04865.1. AB041413 Genomic DNA. Translation: BAA94498.1. AB041416 Genomic DNA. Translation: BAA94501.1.
IPI	IPI00032034.
RefSeq	NP_006048.1. NP_149360.1. NP_149361.1. NP_149362.1. NP_149363.1.
UniGene	Hs.655094
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PeptideAtlas	Q9Y2C3.
PRIDE	Q9Y2C3.
Genome annotation databases
Ensembl	ENSG00000183778. Homo sapiens. [Contig view]
GeneID	10317.
KEGG	hsa:10317.
Organism-specific databases
GeneCards	GC21P039949.
H-InvDB	HIX0040840.
HGNC	HGNC:920. B3GALT5.
MIM	604066. gene.
PharmGKB	PA25213.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9Y2C3.
HOVERGEN	Q9Y2C3.
OMA	Q9Y2C3. LNEFPIR.
Gene expression databases
ArrayExpress	Q9Y2C3.
Bgee	Q9Y2C3.
CleanEx	HS_B3GALT5.
GermOnline	ENSG00000183778. Homo sapiens.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39103.
SOURCE	Search...

Entry information

Entry name

B3GT5_HUMAN

Accession

Primary (citable) accession number: Q9Y2C3
Secondary accession number(s): Q2M3L5 , Q53Z19, Q9NY96, Q9P1X6, Q9P1X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)