ID   CNPY2_HUMAN             Reviewed;         182 AA.
AC   Q9Y2B0; B2R7B9; Q9UHE9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=Protein canopy homolog 2;
DE   AltName: Full=MIR-interacting saposin-like protein;
DE   AltName: Full=Putative secreted protein Zsig9;
DE   AltName: Full=Transmembrane protein 4;
DE   Flags: Precursor;
GN   Name=CNPY2; Synonyms=MSAP, TMEM4, ZSIG9; ORFNames=UNQ1943/PRO4426;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Gastric adenocarcinoma;
RX   PubMed=10072769; DOI=10.1016/s0378-1119(99)00004-9;
RA   Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S.;
RT   "Selection of cDNAs encoding putative type II membrane proteins on the cell
RT   surface from a human full-length cDNA bank.";
RL   Gene 228:161-167(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYLIP, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=12826659; DOI=10.1074/jbc.m306271200;
RA   Bornhauser B.C., Olsson P.-A., Lindholm D.;
RT   "MSAP is a novel MIR-interacting protein that enhances neurite outgrowth
RT   and increases myosin regulatory light chain.";
RL   J. Biol. Chem. 278:35412-35420(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J., O'Hara P.;
RT   "Homo sapiens putative secreted protein.";
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Eye, and Skin;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- FUNCTION: Positive regulator of neurite outgrowth by stabilizing myosin
CC       regulatory light chain (MRLC). It prevents MIR-mediated MRLC
CC       ubiquitination and its subsequent proteasomal degradation.
CC   -!- SUBUNIT: Interacts with MYLIP/MIR. {ECO:0000269|PubMed:12826659}.
CC   -!- INTERACTION:
CC       Q9Y2B0; P18825: ADRA2C; NbExp=3; IntAct=EBI-1054195, EBI-12015266;
CC       Q9Y2B0; Q8WY64: MYLIP; NbExp=3; IntAct=EBI-1054195, EBI-6952711;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE-
CC       ProRule:PRU10138}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9Y2B0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9Y2B0-2; Sequence=VSP_010884, VSP_010885;
CC   -!- TISSUE SPECIFICITY: Expressed in different tissues. Highest levels are
CC       detected in adult placenta, liver and pancreas.
CC       {ECO:0000269|PubMed:12826659}.
CC   -!- SIMILARITY: Belongs to the canopy family. {ECO:0000305}.
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DR   EMBL; AB015631; BAA76498.1; -; mRNA.
DR   EMBL; AY032624; AAK38148.1; -; mRNA.
DR   EMBL; AF186113; AAF01431.1; -; mRNA.
DR   EMBL; AY359102; AAQ89460.1; -; mRNA.
DR   EMBL; AK312921; BAG35766.1; -; mRNA.
DR   EMBL; CH471054; EAW96926.1; -; Genomic_DNA.
DR   EMBL; BC001027; AAH01027.1; -; mRNA.
DR   EMBL; BC065015; AAH65015.1; -; mRNA.
DR   CCDS; CCDS8914.1; -. [Q9Y2B0-1]
DR   RefSeq; NP_001177920.1; NM_001190991.2. [Q9Y2B0-2]
DR   RefSeq; NP_055070.1; NM_014255.6. [Q9Y2B0-1]
DR   AlphaFoldDB; Q9Y2B0; -.
DR   SMR; Q9Y2B0; -.
DR   BioGRID; 115613; 54.
DR   DIP; DIP-50705N; -.
DR   IntAct; Q9Y2B0; 26.
DR   MINT; Q9Y2B0; -.
DR   STRING; 9606.ENSP00000273308; -.
DR   GlyGen; Q9Y2B0; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9Y2B0; -.
DR   PhosphoSitePlus; Q9Y2B0; -.
DR   SwissPalm; Q9Y2B0; -.
DR   BioMuta; CNPY2; -.
DR   DMDM; 50401205; -.
DR   EPD; Q9Y2B0; -.
DR   jPOST; Q9Y2B0; -.
DR   MassIVE; Q9Y2B0; -.
DR   MaxQB; Q9Y2B0; -.
DR   PaxDb; 9606-ENSP00000273308; -.
DR   PeptideAtlas; Q9Y2B0; -.
DR   ProteomicsDB; 85715; -. [Q9Y2B0-1]
DR   ProteomicsDB; 85716; -. [Q9Y2B0-2]
DR   Pumba; Q9Y2B0; -.
DR   TopDownProteomics; Q9Y2B0-1; -. [Q9Y2B0-1]
DR   TopDownProteomics; Q9Y2B0-2; -. [Q9Y2B0-2]
DR   Antibodypedia; 50939; 224 antibodies from 26 providers.
DR   DNASU; 10330; -.
DR   Ensembl; ENST00000273308.9; ENSP00000273308.4; ENSG00000257727.6. [Q9Y2B0-1]
DR   GeneID; 10330; -.
DR   KEGG; hsa:10330; -.
DR   MANE-Select; ENST00000273308.9; ENSP00000273308.4; NM_014255.7; NP_055070.1.
DR   UCSC; uc001sku.3; human. [Q9Y2B0-1]
DR   AGR; HGNC:13529; -.
DR   DisGeNET; 10330; -.
DR   GeneCards; CNPY2; -.
DR   HGNC; HGNC:13529; CNPY2.
DR   HPA; ENSG00000257727; Low tissue specificity.
DR   MIM; 605861; gene.
DR   neXtProt; NX_Q9Y2B0; -.
DR   OpenTargets; ENSG00000257727; -.
DR   PharmGKB; PA162382584; -.
DR   VEuPathDB; HostDB:ENSG00000257727; -.
DR   eggNOG; KOG3782; Eukaryota.
DR   GeneTree; ENSGT00940000161158; -.
DR   HOGENOM; CLU_095726_2_1_1; -.
DR   InParanoid; Q9Y2B0; -.
DR   OMA; VWARRSQ; -.
DR   OrthoDB; 2878740at2759; -.
DR   PhylomeDB; Q9Y2B0; -.
DR   TreeFam; TF318578; -.
DR   PathwayCommons; Q9Y2B0; -.
DR   SignaLink; Q9Y2B0; -.
DR   BioGRID-ORCS; 10330; 93 hits in 1157 CRISPR screens.
DR   ChiTaRS; CNPY2; human.
DR   GenomeRNAi; 10330; -.
DR   Pharos; Q9Y2B0; Tbio.
DR   PRO; PR:Q9Y2B0; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q9Y2B0; Protein.
DR   Bgee; ENSG00000257727; Expressed in adenohypophysis and 200 other cell types or tissues.
DR   ExpressionAtlas; Q9Y2B0; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0010629; P:negative regulation of gene expression; ISS:BHF-UCL.
DR   GO; GO:1905599; P:positive regulation of low-density lipoprotein receptor activity; ISS:BHF-UCL.
DR   GO; GO:0010988; P:regulation of low-density lipoprotein particle clearance; ISS:BHF-UCL.
DR   Gene3D; 1.10.225.10; Saposin-like; 1.
DR   InterPro; IPR042415; CNPY.
DR   InterPro; IPR021852; DUF3456.
DR   InterPro; IPR008139; SaposinB_dom.
DR   PANTHER; PTHR13341; MIR-INTERACTING SAPOSIN-LIKE PROTEIN; 1.
DR   PANTHER; PTHR13341:SF6; PROTEIN CANOPY HOMOLOG 2; 1.
DR   Pfam; PF11938; DUF3456; 1.
DR   PROSITE; PS00014; ER_TARGET; 1.
DR   PROSITE; PS50015; SAP_B; 1.
DR   Genevisible; Q9Y2B0; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Disulfide bond; Endoplasmic reticulum;
KW   Phosphoprotein; Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..182
FT                   /note="Protein canopy homolog 2"
FT                   /id="PRO_0000031666"
FT   DOMAIN          24..175
FT                   /note="Saposin B-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   MOTIF           179..182
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT   MOD_RES         115
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9QXT0"
FT   DISULFID        28..171
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        31..164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        86..137
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   VAR_SEQ         70..84
FT                   /note="PYARSEAHLTELLEE -> TVTVPPNKVAHSGFG (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT                   /id="VSP_010884"
FT   VAR_SEQ         85..182
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT                   /id="VSP_010885"
SQ   SEQUENCE   182 AA;  20652 MW;  BE726D302490733F CRC64;
     MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP
     DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL
     QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD
     EL
//