Q9Y2B0 (CNPY2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein canopy homolog 2

Alternative name(s):
MIR-interacting saposin-like protein
Putative secreted protein Zsig9
Transmembrane protein 4

Gene names

Name:	CNPY2
Synonyms:	MSAP, TMEM4, ZSIG9
ORF Names:	UNQ1943/PRO4426

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.
Subunit structure	Interacts with MYLIP/MIR. Ref.2
Subcellular location	Endoplasmic reticulum Potential.
Tissue specificity	Expressed in different tissues. Highest levels are detected in adult placenta, liver and pancreas. Ref.2
Sequence similarities	Belongs to the canopy family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of gene expression Inferred from sequence or structural similarity PubMed 22378787. Source: BHF-UCL positive regulation of low-density lipoprotein particle receptor biosynthetic process Inferred from sequence or structural similarity PubMed 22378787. Source: BHF-UCL regulation of low-density lipoprotein particle clearance Inferred from sequence or structural similarity PubMed 22378787. Source: BHF-UCL
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2B0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2B0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-84: PYARSEAHLTELLEE → TVTVPPNKVAHSGFG
     85-182: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 182	162	Protein canopy homolog 2		PRO_0000031666
Regions
	Domain	24 – 175	152	Saposin B-type
	Motif	179 – 182	4	Prevents secretion from ER Potential
Amino acid modifications
	Disulfide bond	28 ↔ 171		By similarity
	Disulfide bond	31 ↔ 164		By similarity
	Disulfide bond	86 ↔ 137		By similarity
Natural variations
	Alternative sequence	70 – 84	15	PYARS…ELLEE → TVTVPPNKVAHSGFG in isoform 2.		VSP_010884
	Alternative sequence	85 – 182	98	Missing in isoform 2.		VSP_010885

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: BE726D302490733F	FASTA	182	20,652
	10 20 30 40 50 60 MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP 70 80 90 100 110 120 DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL 130 140 150 160 170 180 QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD EL
	Isoform 2 [UniParc]. Checksum: DCA2B08EC77EF1F0	FASTA	84	9,117
	10 20 30 40 50 60 MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP 70 80 DGSQSVVEVT VTVPPNKVAH SGFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank." Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S. Gene 228:161-167(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Gastric adenocarcinoma.
[2]	"MSAP is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain." Bornhauser B.C., Olsson P.-A., Lindholm D. J. Biol. Chem. 278:35412-35420(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYLIP, TISSUE SPECIFICITY.
[3]	"Homo sapiens putative secreted protein." Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J., O'Hara P. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015631 mRNA. Translation: BAA76498.1. AY032624 mRNA. Translation: AAK38148.1. AF186113 mRNA. Translation: AAF01431.1. AY359102 mRNA. Translation: AAQ89460.1. AK312921 mRNA. Translation: BAG35766.1. CH471054 Genomic DNA. Translation: EAW96926.1. BC001027 mRNA. Translation: AAH01027.1. BC065015 mRNA. Translation: AAH65015.1.
IPI	IPI00383952. IPI00443909.
RefSeq	NP_001177920.1. NM_001190991.1. NP_055070.1. NM_014255.5.
UniGene	Hs.8752.
3D structure databases
ProteinModelPortal	Q9Y2B0.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-50705N.
IntAct	Q9Y2B0. 1 interaction.
STRING	9606.ENSP00000273308.
PTM databases
PhosphoSite	Q9Y2B0.
Polymorphism databases
DMDM	50401205.
Proteomic databases
PaxDb	Q9Y2B0.
PeptideAtlas	Q9Y2B0.
PRIDE	Q9Y2B0.
Protocols and materials databases
DNASU	10330.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000273308; ENSP00000273308; ENSG00000257727.
GeneID	10330.
KEGG	hsa:10330.
UCSC	uc001sku.2. human. uc001skv.3. human.
Organism-specific databases
CTD	10330.
GeneCards	GC12M056704.
HGNC	HGNC:13529. CNPY2.
HPA	HPA038465. HPA038466.
MIM	605861. gene.
neXtProt	NX_Q9Y2B0.
PharmGKB	PA162382584.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG280279.
HOGENOM	HOG000233996.
HOVERGEN	HBG052514.
InParanoid	Q9Y2B0.
OrthoDB	EOG45490T.
PhylomeDB	Q9Y2B0.
Gene expression databases
ArrayExpress	Q9Y2B0.
Bgee	Q9Y2B0.
CleanEx	HS_CNPY2.
Genevestigator	Q9Y2B0.
GermOnline	ENSG00000144785. Homo sapiens.
Family and domain databases
InterPro	IPR021852. DUF3456. IPR008139. SaposinB. [Graphical view]
Pfam	PF11938. DUF3456. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. PS50015. SAP_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CNPY2. human.
GenomeRNAi	10330.
NextBio	39165.
SOURCE	Search...

Entry information

Entry name

CNPY2_HUMAN

Accession

Primary (citable) accession number: Q9Y2B0
Secondary accession number(s): B2R7B9, Q9UHE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.