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Q9Y2B0 (CNPY2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein canopy homolog 2
Alternative name(s):
MIR-interacting saposin-like protein
Putative secreted protein Zsig9
Transmembrane protein 4
Gene names
Name:CNPY2
Synonyms:MSAP, TMEM4, ZSIG9
ORF Names:UNQ1943/PRO4426
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.

Subunit structure

Interacts with MYLIP/MIR. Ref.2

Subcellular location

Endoplasmic reticulum Potential.

Tissue specificity

Expressed in different tissues. Highest levels are detected in adult placenta, liver and pancreas. Ref.2

Sequence similarities

Belongs to the canopy family.

Contains 1 saposin B-type domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y2B0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y2B0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-84: PYARSEAHLTELLEE → TVTVPPNKVAHSGFG
     85-182: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 182162Protein canopy homolog 2
PRO_0000031666

Regions

Domain24 – 175152Saposin B-type
Motif179 – 1824Prevents secretion from ER Potential

Amino acid modifications

Modified residue1151Phosphoserine By similarity
Disulfide bond28 ↔ 171 By similarity
Disulfide bond31 ↔ 164 By similarity
Disulfide bond86 ↔ 137 By similarity

Natural variations

Alternative sequence70 – 8415PYARS…ELLEE → TVTVPPNKVAHSGFG in isoform 2.
VSP_010884
Alternative sequence85 – 18298Missing in isoform 2.
VSP_010885

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: BE726D302490733F

FASTA18220,652
        10         20         30         40         50         60 
MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP 

        70         80         90        100        110        120 
DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL 

       130        140        150        160        170        180 
QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD 


EL 

« Hide

Isoform 2 [UniParc].

Checksum: DCA2B08EC77EF1F0
Show »

FASTA849,117

References

« Hide 'large scale' references
[1]"Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank."
Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S.
Gene 228:161-167(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Gastric adenocarcinoma.
[2]"MSAP is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain."
Bornhauser B.C., Olsson P.-A., Lindholm D.
J. Biol. Chem. 278:35412-35420(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYLIP, TISSUE SPECIFICITY.
[3]"Homo sapiens putative secreted protein."
Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J., O'Hara P.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Eye and Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015631 mRNA. Translation: BAA76498.1.
AY032624 mRNA. Translation: AAK38148.1.
AF186113 mRNA. Translation: AAF01431.1.
AY359102 mRNA. Translation: AAQ89460.1.
AK312921 mRNA. Translation: BAG35766.1.
CH471054 Genomic DNA. Translation: EAW96926.1.
BC001027 mRNA. Translation: AAH01027.1.
BC065015 mRNA. Translation: AAH65015.1.
RefSeqNP_001177920.1. NM_001190991.1.
NP_055070.1. NM_014255.5.
UniGeneHs.8752.

3D structure databases

ProteinModelPortalQ9Y2B0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115613. 3 interactions.
DIPDIP-50705N.
IntActQ9Y2B0. 1 interaction.
MINTMINT-3084309.
STRING9606.ENSP00000273308.

PTM databases

PhosphoSiteQ9Y2B0.

Polymorphism databases

DMDM50401205.

Proteomic databases

PaxDbQ9Y2B0.
PeptideAtlasQ9Y2B0.
PRIDEQ9Y2B0.

Protocols and materials databases

DNASU10330.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273308; ENSP00000273308; ENSG00000257727. [Q9Y2B0-1]
GeneID10330.
KEGGhsa:10330.
UCSCuc001sku.2. human. [Q9Y2B0-1]
uc001skv.3. human. [Q9Y2B0-2]

Organism-specific databases

CTD10330.
GeneCardsGC12M056704.
HGNCHGNC:13529. CNPY2.
HPAHPA038465.
HPA038466.
MIM605861. gene.
neXtProtNX_Q9Y2B0.
PharmGKBPA162382584.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG280279.
HOGENOMHOG000233996.
HOVERGENHBG052514.
InParanoidQ9Y2B0.
PhylomeDBQ9Y2B0.
TreeFamTF318578.

Gene expression databases

ArrayExpressQ9Y2B0.
BgeeQ9Y2B0.
CleanExHS_CNPY2.
GenevestigatorQ9Y2B0.

Family and domain databases

InterProIPR021852. DUF3456.
IPR008139. SaposinB.
[Graphical view]
PfamPF11938. DUF3456. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCNPY2. human.
GenomeRNAi10330.
NextBio39165.
PROQ9Y2B0.
SOURCESearch...

Entry information

Entry nameCNPY2_HUMAN
AccessionPrimary (citable) accession number: Q9Y2B0
Secondary accession number(s): B2R7B9, Q9UHE9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1999
Last modified: March 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM