Q9Y2A9 (B3GN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Short name=BGnT-3
Short name=Beta-1,3-Gn-T3
Short name=Beta-1,3-N-acetylglucosaminyltransferase 3
Short name=Beta3Gn-T3
EC=2.4.1.-

Alternative name(s):
Beta-1,3-galactosyltransferase 8
Short name=Beta-1,3-GalTase 8
Short name=Beta3Gal-T8
Short name=Beta3GalT8
Short name=b3Gal-T8
Beta-3-Gx-T8
Core 1 extending beta-1,3-N-acetylglucosaminyltransferase
Core1-beta3GlcNAcT
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8

Gene names

Name:	B3GNT3
Synonyms:	B3GALT8, TMEM3
ORF Names:	UNQ637/PRO1266

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a beta-1,3-N-acetylglucosaminyltransferase activity for type 2 oligosaccharides. Ref.3
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in colon, jejunum, stomach, esophagus, placenta and trachea. Ref.3
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence CAC82374.1 differs from that shown. Reason: Frameshift at positions 194, 196, 202, 204, 209 and 215.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3		PRO_0000219172
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 372	341	Lumenal Potential
Amino acid modifications
	Modified residue	276	1	Phosphoserine Ref.11
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	328	1	R → H. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6 Ref.9 Corresponds to variant rs36686 [ dbSNP | Ensembl ].		VAR_022644
Experimental info
	Sequence conflict	89	1	C → Y in AAH66876. Ref.9
	Sequence conflict	355	1	W → R in AAH67423. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2A9 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: A6E3FE88B2F00F10	FASTA	372	42,534
	10 20 30 40 50 60 MKYLRHRRPN ATLILAIGAF TLLLFSLLVS PPTCKVQEQP PAIPEALAWP TPPTRPAPAP 70 80 90 100 110 120 CHANTSMVTH PDFATQPQHV QNFLLYRHCR HFPLLQDVPP SKCAQPVFLL LVIKSSPSNY 130 140 150 160 170 180 VRRELLRRTW GRERKVRGLQ LRLLFLVGTA SNPHEARKVN RLLELEAQTH GDILQWDFHD 190 200 210 220 230 240 SFFNLTLKQV LFLQWQETRC ANASFVLNGD DDVFAHTDNM VFYLQDHDPG RHLFVGQLIQ 250 260 270 280 290 300 NVGPIRAFWS KYYVPEVVTQ NERYPPYCGG GGFLLSRFTA AALRRAAHVL DIFPIDDVFL 310 320 330 340 350 360 GMCLELEGLK PASHSGIRTS GVRAPSQRLS SFDPCFYRDL LLVHRFLPYE MLLMWDALNQ 370 PNLTCGNQTQ IY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank." Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S. Gene 228:161-167(1999) [PubMed: 10072769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[2]	"Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase." Yeh J.-C., Hiraoka N., Petryniak B., Nakayama J., Ellies L.G., Rabuka D., Hindsgaul O., Marth J.D., Lowe J.B., Fakuda M. Cell 105:957-969(2001) [PubMed: 11439191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[3]	"Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family." Shiraishi N., Natsume A., Togayachi A., Endo T., Akashima T., Yamada Y., Imai N., Nakagawa S., Koizumi S., Sekine S., Narimatsu H., Sasaki K. J. Biol. Chem. 276:3498-3507(2001) [PubMed: 11042166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, TISSUE SPECIFICITY. Tissue: Gastric mucosa.
[4]	"Cloning of a new member of the beta 1,3 galactosyltransferase family, b1,3Gal-T6." Jensen M.A., Bennett E.P. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[5]	Bennett E.P. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[6]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-328.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[8]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-328.
[10]	"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." Amado M., Almeida R., Schwientek T., Clausen H. Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract] Cited for: REVIEW.
[11]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015630 mRNA. Translation: BAA76497.1. AF293973 mRNA. Translation: AAK00849.1. AB049585 mRNA. Translation: BAB21531.1. AJ130847 mRNA. Translation: CAC45044.1. AJ278961 mRNA. Translation: CAC82374.1. Frameshift. AY358955 mRNA. Translation: AAQ89314.1. AK314323 mRNA. Translation: BAG36971.1. AC008761 Genomic DNA. No translation available. BC066876 mRNA. Translation: AAH66876.1. BC067423 mRNA. Translation: AAH67423.1.
IPI	IPI00031983.
RefSeq	NP_055071.2. NM_014256.3.
UniGene	Hs.657825. Hs.69009.
3D structure databases
ProteinModelPortal	Q9Y2A9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y2A9.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Polymorphism databases
DMDM	311033352.
Proteomic databases
PRIDE	Q9Y2A9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000318683; ENSP00000321874; ENSG00000179913.
GeneID	10331.
KEGG	hsa:10331.
Organism-specific databases
CTD	10331.
GeneCards	GC19P017905.
HGNC	HGNC:13528. B3GNT3.
HPA	HPA024298.
MIM	605863. gene.
neXtProt	NX_Q9Y2A9.
PharmGKB	PA25219.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08192.
GeneTree	ENSGT00560000076821.
HOGENOM	HBG714704.
HOVERGEN	HBG050653.
InParanoid	Q9Y2A9.
OMA	CAQPVFL.
OrthoDB	EOG4D52Z3.
PhylomeDB	Q9Y2A9.
Enzyme and pathway databases
BRENDA	2.4.1.146. 2681.
Gene expression databases
ArrayExpress	Q9Y2A9.
Bgee	Q9Y2A9.
CleanEx	HS_B3GNT3.
Genevestigator	Q9Y2A9.
GermOnline	ENSG00000179913. Homo sapiens.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
KO	K07970.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39169.
SOURCE	Search...

Entry information

Entry name

B3GN3_HUMAN

Accession

Primary (citable) accession number: Q9Y2A9
Secondary accession number(s): B2RAS4 , Q6NWU9, Q6NXU9, Q8WWR6, Q9C0J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 2, 2010
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.