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Q9Y2A9 (B3GN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Short name=BGnT-3
Short name=Beta-1,3-Gn-T3
Short name=Beta-1,3-N-acetylglucosaminyltransferase 3
Short name=Beta3Gn-T3
EC=2.4.1.146
Alternative name(s):
Beta-1,3-galactosyltransferase 8
Short name=Beta-1,3-GalTase 8
Short name=Beta3Gal-T8
Short name=Beta3GalT8
Short name=b3Gal-T8
Beta-3-Gx-T8
Core 1 extending beta-1,3-N-acetylglucosaminyltransferase
Core1-beta3GlcNAcT
Transmembrane protein 3
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8
Gene names
Name:B3GNT3
Synonyms:B3GALT8, TMEM3
ORF Names:UNQ637/PRO1266
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a beta-1,3-N-acetylglucosaminyltransferase activity for type 2 oligosaccharides. Ref.3

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->3)-(N-acetyl-D-glucosaminyl-(1->6))-N-acetyl-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-(N-acetyl-D-glucosaminyl-(1->6))-N-acetyl-D-galactosaminyl-R. Ref.3

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Expressed in colon, jejunum, stomach, esophagus, placenta and trachea. Ref.3

Sequence similarities

Belongs to the glycosyltransferase 31 family.

Sequence caution

The sequence CAC82374.1 differs from that shown. Reason: Frameshift at positions 194, 196, 202, 204, 209 and 215.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
PRO_0000219172

Regions

Topological domain1 – 1010Cytoplasmic Potential
Transmembrane11 – 3121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain32 – 372341Lumenal Potential

Amino acid modifications

Glycosylation641N-linked (GlcNAc...) Potential
Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation3621N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential

Natural variations

Natural variant3281R → H. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6 Ref.9
Corresponds to variant rs36686 [ dbSNP | Ensembl ].
VAR_022644

Experimental info

Sequence conflict891C → Y in AAH66876. Ref.9
Sequence conflict3551W → R in AAH67423. Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q9Y2A9 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: A6E3FE88B2F00F10

FASTA37242,534
        10         20         30         40         50         60 
MKYLRHRRPN ATLILAIGAF TLLLFSLLVS PPTCKVQEQP PAIPEALAWP TPPTRPAPAP 

        70         80         90        100        110        120 
CHANTSMVTH PDFATQPQHV QNFLLYRHCR HFPLLQDVPP SKCAQPVFLL LVIKSSPSNY 

       130        140        150        160        170        180 
VRRELLRRTW GRERKVRGLQ LRLLFLVGTA SNPHEARKVN RLLELEAQTH GDILQWDFHD 

       190        200        210        220        230        240 
SFFNLTLKQV LFLQWQETRC ANASFVLNGD DDVFAHTDNM VFYLQDHDPG RHLFVGQLIQ 

       250        260        270        280        290        300 
NVGPIRAFWS KYYVPEVVTQ NERYPPYCGG GGFLLSRFTA AALRRAAHVL DIFPIDDVFL 

       310        320        330        340        350        360 
GMCLELEGLK PASHSGIRTS GVRAPSQRLS SFDPCFYRDL LLVHRFLPYE MLLMWDALNQ 

       370 
PNLTCGNQTQ IY 

« Hide

References

« Hide 'large scale' references
[1]"Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank."
Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S.
Gene 228:161-167(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[2]"Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase."
Yeh J.-C., Hiraoka N., Petryniak B., Nakayama J., Ellies L.G., Rabuka D., Hindsgaul O., Marth J.D., Lowe J.B., Fakuda M.
Cell 105:957-969(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[3]"Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family."
Shiraishi N., Natsume A., Togayachi A., Endo T., Akashima T., Yamada Y., Imai N., Nakagawa S., Koizumi S., Sekine S., Narimatsu H., Sasaki K.
J. Biol. Chem. 276:3498-3507(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, TISSUE SPECIFICITY.
Tissue: Gastric mucosa.
[4]"Cloning of a new member of the beta 1,3 galactosyltransferase family, b1,3Gal-T6."
Jensen M.A., Bennett E.P.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[5]Bennett E.P.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-328.
[6]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-328.
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[8]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-328.
[10]"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
Amado M., Almeida R., Schwientek T., Clausen H.
Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015630 mRNA. Translation: BAA76497.1.
AF293973 mRNA. Translation: AAK00849.1.
AB049585 mRNA. Translation: BAB21531.1.
AJ130847 mRNA. Translation: CAC45044.1.
AJ278961 mRNA. Translation: CAC82374.1. Frameshift.
AY358955 mRNA. Translation: AAQ89314.1.
AK314323 mRNA. Translation: BAG36971.1.
AC008761 Genomic DNA. No translation available.
BC066876 mRNA. Translation: AAH66876.1.
BC067423 mRNA. Translation: AAH67423.1.
RefSeqNP_055071.2. NM_014256.3.
UniGeneHs.657825.
Hs.69009.

3D structure databases

ProteinModelPortalQ9Y2A9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000321874.

Protein family/group databases

CAZyGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSiteQ9Y2A9.

Polymorphism databases

DMDM311033352.

Proteomic databases

PaxDbQ9Y2A9.
PRIDEQ9Y2A9.

Protocols and materials databases

DNASU10331.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000318683; ENSP00000321874; ENSG00000179913.
ENST00000595387; ENSP00000472638; ENSG00000179913.
GeneID10331.
KEGGhsa:10331.
UCSCuc002nhl.1. human.

Organism-specific databases

CTD10331.
GeneCardsGC19P017905.
HGNCHGNC:13528. B3GNT3.
HPAHPA024298.
MIM605863. gene.
neXtProtNX_Q9Y2A9.
PharmGKBPA25219.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293830.
HOGENOMHOG000232195.
HOVERGENHBG050653.
InParanoidQ9Y2A9.
KOK07970.
OMACAQPVFL.
PhylomeDBQ9Y2A9.
TreeFamTF318639.

Enzyme and pathway databases

BRENDA2.4.1.146. 2681.
ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
REACT_17015. Metabolism of proteins.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ9Y2A9.
BgeeQ9Y2A9.
CleanExHS_B3GNT3.
GenevestigatorQ9Y2A9.

Family and domain databases

InterProIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERPTHR11214. PTHR11214. 1 hit.
PfamPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiBeta-1,3-N-acetylglucosaminyltransferase_3.
GenomeRNAi10331.
NextBio39169.
PROQ9Y2A9.
SOURCESearch...

Entry information

Entry nameB3GN3_HUMAN
AccessionPrimary (citable) accession number: Q9Y2A9
Secondary accession number(s): B2RAS4 expand/collapse secondary AC list , Q6NWU9, Q6NXU9, Q8WWR6, Q9C0J2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 2, 2010
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM