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Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3

Gene

B3GNT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides (PubMed:11042166). Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans (PubMed:11439191).2 Publications

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O3-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O3-(N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein].1 Publication
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000179913-MONOMER
BRENDAi2.4.1.146 2681
ReactomeiR-HSA-2022854 Keratan sulfate biosynthesis
R-HSA-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC:2.4.1.1491 Publication)
Alternative name(s):
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (EC:2.4.1.1461 Publication)
Beta-1,3-galactosyltransferase 8
Short name:
Beta-1,3-GalTase 8
Short name:
Beta3Gal-T8
Short name:
Beta3GalT8
Short name:
b3Gal-T8
Beta-3-Gx-T8
Core 1 extending beta-1,3-N-acetylglucosaminyltransferase1 Publication
Core1-beta3GlcNAcT1 Publication
Transmembrane protein 3
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Short name:
BGnT-3
Short name:
Beta-1,3-Gn-T3
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 3
Short name:
Beta3Gn-T3
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8
Gene namesi
Name:B3GNT3
Synonyms:B3GALT8, TMEM3
ORF Names:UNQ637/PRO1266
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000179913.10
HGNCiHGNC:13528 B3GNT3
MIMi605863 gene
neXtProtiNX_Q9Y2A9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10CytoplasmicSequence analysis10
Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini32 – 372LumenalSequence analysisAdd BLAST341

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10331
OpenTargetsiENSG00000179913
PharmGKBiPA25219

Chemistry databases

ChEMBLiCHEMBL3325305

Polymorphism and mutation databases

BioMutaiB3GNT3
DMDMi311033352

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191721 – 372N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi362N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9Y2A9
PaxDbiQ9Y2A9
PeptideAtlasiQ9Y2A9
PRIDEiQ9Y2A9

PTM databases

iPTMnetiQ9Y2A9
PhosphoSitePlusiQ9Y2A9

Expressioni

Tissue specificityi

Expressed in colon, jejunum, stomach, esophagus, placenta and trachea.1 Publication

Gene expression databases

BgeeiENSG00000179913
CleanExiHS_B3GNT3
ExpressionAtlasiQ9Y2A9 baseline and differential
GenevisibleiQ9Y2A9 HS

Organism-specific databases

HPAiHPA024298

Interactioni

Protein-protein interaction databases

BioGridi115614, 23 interactors
STRINGi9606.ENSP00000321874

Chemistry databases

BindingDBiQ9Y2A9

Structurei

3D structure databases

ProteinModelPortaliQ9Y2A9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00760000118879
HOGENOMiHOG000232195
HOVERGENiHBG050653
InParanoidiQ9Y2A9
KOiK07970
OMAiYKHCRDF
OrthoDBiEOG091G0AXM
PhylomeDBiQ9Y2A9
TreeFamiTF318639

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Y2A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYLRHRRPN ATLILAIGAF TLLLFSLLVS PPTCKVQEQP PAIPEALAWP
60 70 80 90 100
TPPTRPAPAP CHANTSMVTH PDFATQPQHV QNFLLYRHCR HFPLLQDVPP
110 120 130 140 150
SKCAQPVFLL LVIKSSPSNY VRRELLRRTW GRERKVRGLQ LRLLFLVGTA
160 170 180 190 200
SNPHEARKVN RLLELEAQTH GDILQWDFHD SFFNLTLKQV LFLQWQETRC
210 220 230 240 250
ANASFVLNGD DDVFAHTDNM VFYLQDHDPG RHLFVGQLIQ NVGPIRAFWS
260 270 280 290 300
KYYVPEVVTQ NERYPPYCGG GGFLLSRFTA AALRRAAHVL DIFPIDDVFL
310 320 330 340 350
GMCLELEGLK PASHSGIRTS GVRAPSQRLS SFDPCFYRDL LLVHRFLPYE
360 370
MLLMWDALNQ PNLTCGNQTQ IY
Length:372
Mass (Da):42,534
Last modified:November 2, 2010 - v2
Checksum:iA6E3FE88B2F00F10
GO

Sequence cautioni

The sequence CAC82374 differs from that shown. Reason: Frameshift at positions 194, 196, 202, 204, 209 and 215.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89C → Y in AAH66876 (PubMed:15489334).Curated1
Sequence conflicti355W → R in AAH67423 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_074178317I → F1 Publication1
Natural variantiVAR_022644328R → H6 PublicationsCorresponds to variant dbSNP:rs36686Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015630 mRNA Translation: BAA76497.1
AF293973 mRNA Translation: AAK00849.1
AB049585 mRNA Translation: BAB21531.1
AJ130847 mRNA Translation: CAC45044.1
AJ278961 mRNA Translation: CAC82374.1 Frameshift.
AY358955 mRNA Translation: AAQ89314.1
AK314323 mRNA Translation: BAG36971.1
AC008761 Genomic DNA No translation available.
BC066876 mRNA Translation: AAH66876.1
BC067423 mRNA Translation: AAH67423.1
CCDSiCCDS12364.1
RefSeqiNP_055071.2, NM_014256.3
XP_011525928.1, XM_011527626.2
UniGeneiHs.657825
Hs.69009

Genome annotation databases

EnsembliENST00000318683; ENSP00000321874; ENSG00000179913
ENST00000595387; ENSP00000472638; ENSG00000179913
GeneIDi10331
KEGGihsa:10331
UCSCiuc002nhl.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiB3GN3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2A9
Secondary accession number(s): B2RAS4
, Q6NWU9, Q6NXU9, Q8WWR6, Q9C0J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 2, 2010
Last modified: April 25, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health