Reviewed, UniProtKB/Swiss-Prot Q9Y2A7 (NCKP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Nck-associated protein 1
      Short name=NAP 1
Alternative name(s):
    p125Nap1
    Membrane-associated protein HEM-2

Gene names

Name:	NCKAP1
Synonyms:	HEM2, KIAA0587, NAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1128 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is part of lamellipodial complex that controls Rac-dependent actin remodeling By similarity.
Subunit structure	Associates preferentially with the first SH3 domain of NCK. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Also a component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein; Cytoplasmic sideBy similarity. Cell projection › lamellipodium membrane; Single-pass membrane protein; Cytoplasmic sideBy similarity. Note= At the interface between the lamellipodial actin meshwork and the membrane By similarity.
Tissue specificity	Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
Sequence similarities	Belongs to the HEM-1/HEM-2 family.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Membrane
Domain	Transmembrane
Gene Ontology (GO)
Biological process	apoptosis Ref.1 Traceable author statement. Source: ProtInc central nervous system development Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABI1	Q8IZP0	2	EBI-389845,EBI-375446
CYFIP1	Q7L576	2	EBI-389845,EBI-1048143
YWHAB	P31946	1	EBI-389845,EBI-359815
YWHAZ	P63104	1	EBI-389845,EBI-347088

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1128	1128	Nck-associated protein 1		PRO_0000216172
Regions
	Transmembrane	995 – 1015	21	Potential
Experimental info
	Sequence conflict	36	1	K → KQGQVWK Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2A7-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 12D32BAC7080CB5A	FASTA	1,128	128,790
	10 20 30 40 50 60 MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI 70 80 90 100 110 120 VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDVCQVF 130 140 150 160 170 180 FDITVNFDLT KNYLDLIITY TTLMILLSRI EERKAIIGLY NYAHEMTHGA SDREYPRLGQ 190 200 210 220 230 240 MIVDYENPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML 250 260 270 280 290 300 NPAQSDTMPC EYLSLDAMEK WIIFGFILCH GILNTDATAL NLWKLALQSS SCLSLFRDEV 310 320 330 340 350 360 FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL KELATVLSDQ 370 380 390 400 410 420 PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF IDKHIAELIF YMEELRAHVR 430 440 450 460 470 480 KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV 490 500 510 520 530 540 FDFRGMRLDW FRLQAYTSVS KASLGLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS 550 560 570 580 590 600 IFCFYSRAFE KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN 610 620 630 640 650 660 MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP 670 680 690 700 710 720 GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMVVWEH TFTPREYLTS HLEIRFTKSI 730 740 750 760 770 780 VGMTMYNQAT QEIAKPSELL TSVRAYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG 790 800 810 820 830 840 EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS 850 860 870 880 890 900 ELLGPYGMKF LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV 910 920 930 940 950 960 DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET DMKVAMNVYE 970 980 990 1000 1010 1020 LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM VFVAVSLPTL ASNVMSQYSP 1030 1040 1050 1060 1070 1080 AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN 1090 1100 1110 1120 RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVTSSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease." Suzuki T., Nishiyama K., Yamamoto A., Inazawa J., Iwaki T., Yamada T., Kanazawa I., Sakaki Y. Genomics 63:246-254(2000) [PubMed: 10673335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB014509 mRNA. Translation: BAA77295.1. AB011159 mRNA. Translation: BAA25513.2. Different initiation. BC015025 mRNA. Translation: AAH15025.1.
RefSeq	NP_038464.1. NP_995314.1.
UniGene	Hs.603732
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y2A7.
PTM databases
PhosphoSite	Q9Y2A7.
Genome annotation databases
Ensembl	ENSG00000061676. Homo sapiens. [Contig view]
GeneID	10787.
KEGG	hsa:10787.
Organism-specific databases
H-InvDB	HIX0018862.
HGNC	HGNC:7666. NCKAP1.
MIM	604891. gene.
PharmGKB	PA31468.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q9Y2A7.
Gene expression databases
ArrayExpress	Q9Y2A7.
CleanEx	HS_NCKAP1.
GermOnline	ENSG00000061676. Homo sapiens.
Family and domain databases
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	Q9Y2A7.
NextBio	40972.
SOURCE	Search...

Entry information

Entry name

NCKP1_HUMAN

Accession

Primary (citable) accession number: Q9Y2A7
Secondary accession number(s): O60329

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)