Reviewed, UniProtKB/Swiss-Prot Q9Y2A7 (NCKP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Nck-associated protein 1
      Short name=NAP 1
Alternative name(s):
    p125Nap1
    Membrane-associated protein HEM-2

Gene names

Name:	NCKAP1
Synonyms:	HEM2, KIAA0587, NAP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1128 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is part of lamellipodial complex that controls Rac-dependent actin remodeling By similarity.
Subunit structure	Associates preferentially with the first SH3 domain of NCK. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Also a component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Cell projection › lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Note: At the interface between the lamellipodial actin meshwork and the membrane By similarity.
Tissue specificity	Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
Sequence similarities	Belongs to the HEM-1/HEM-2 family.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Ref.1 Traceable author statement. Source: ProtInc central nervous system development Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cell projection Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABI1	Q8IZP0	2	EBI-389845,EBI-375446
CYFIP1	Q7L576	2	EBI-389845,EBI-1048143
YWHAB	P31946	1	EBI-389845,EBI-359815
YWHAZ	P63104	1	EBI-389845,EBI-347088

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2A7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2A7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-36: K → KQGQVWK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1128	1128	Nck-associated protein 1		PRO_0000216172
Regions
	Transmembrane	995 – 1015	21	Potential
Natural variations
	Alternative sequence	36	1	K → KQGQVWK in isoform 2.		VSP_036558

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 12D32BAC7080CB5A	FASTA	1,128	128,790
	10 20 30 40 50 60 MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI 70 80 90 100 110 120 VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDVCQVF 130 140 150 160 170 180 FDITVNFDLT KNYLDLIITY TTLMILLSRI EERKAIIGLY NYAHEMTHGA SDREYPRLGQ 190 200 210 220 230 240 MIVDYENPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML 250 260 270 280 290 300 NPAQSDTMPC EYLSLDAMEK WIIFGFILCH GILNTDATAL NLWKLALQSS SCLSLFRDEV 310 320 330 340 350 360 FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL KELATVLSDQ 370 380 390 400 410 420 PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF IDKHIAELIF YMEELRAHVR 430 440 450 460 470 480 KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV 490 500 510 520 530 540 FDFRGMRLDW FRLQAYTSVS KASLGLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS 550 560 570 580 590 600 IFCFYSRAFE KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN 610 620 630 640 650 660 MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP 670 680 690 700 710 720 GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMVVWEH TFTPREYLTS HLEIRFTKSI 730 740 750 760 770 780 VGMTMYNQAT QEIAKPSELL TSVRAYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG 790 800 810 820 830 840 EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS 850 860 870 880 890 900 ELLGPYGMKF LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV 910 920 930 940 950 960 DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET DMKVAMNVYE 970 980 990 1000 1010 1020 LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM VFVAVSLPTL ASNVMSQYSP 1030 1040 1050 1060 1070 1080 AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN 1090 1100 1110 1120 RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVTSSA
	Isoform 2. Checksum: 71019B9B0BCA760D	FASTA	1,134	129,517
	10 20 30 40 50 60 MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKQGQV WKACGDPKAK PSYLIDKNLE 70 80 90 100 110 120 SAVKFIVRKF PAVETRNNNQ QLAQLQKEKS EILKNLALYY FTFVDVMEFK DHVCELLNTI 130 140 150 160 170 180 DVCQVFFDIT VNFDLTKNYL DLIITYTTLM ILLSRIEERK AIIGLYNYAH EMTHGASDRE 190 200 210 220 230 240 YPRLGQMIVD YENPLKKMME EFVPHSKSLS DALISLQMVY PRRNLSADQW RNAQLLSLIS 250 260 270 280 290 300 APSTMLNPAQ SDTMPCEYLS LDAMEKWIIF GFILCHGILN TDATALNLWK LALQSSSCLS 310 320 330 340 350 360 LFRDEVFHIH KAAEDLFVNI RGYNKRINDI RECKEAAVSH AGSMHRERRK FLRSALKELA 370 380 390 400 410 420 TVLSDQPGLL GPKALFVFMA LSFARDEIIW LLRHADNMPK KSADDFIDKH IAELIFYMEE 430 440 450 460 470 480 LRAHVRKYGP VMQRYYVQYL SGFDAVVLNE LVQNLSVCPE DESIIMSSFV NTMTSLSVKQ 490 500 510 520 530 540 VEDGEVFDFR GMRLDWFRLQ AYTSVSKASL GLADHRELGK MMNTIIFHTK MVDSLVEMLV 550 560 570 580 590 600 ETSDLSIFCF YSRAFEKMFQ QCLELPSQSR YSIAFPLLCT HFMSCTHELC PEERHHIGDR 610 620 630 640 650 660 SLSLCNMFLD EMAKQARNLI TDICTEQCTL SDQLLPKHCA KTISQAVNKK SKKQTGKKGE 670 680 690 700 710 720 PEREKPGVES MRKNRLVVTN LDKLHTALSE LCFSINYVPN MVVWEHTFTP REYLTSHLEI 730 740 750 760 770 780 RFTKSIVGMT MYNQATQEIA KPSELLTSVR AYMTVLQSIE NYVQIDITRV FNNVLLQQTQ 790 800 810 820 830 840 HLDSHGEPTI TSLYTNWYLE TLLRQVSNGH IAYFPAMKAF VNLPTENELT FNAEEYSDIS 850 860 870 880 890 900 EMRSLSELLG PYGMKFLSES LMWHISSQVA ELKKLVVENV DVLTQMRTSF DKPDQMAALF 910 920 930 940 950 960 KRLSSVDSVL KRMTIIGVIL SFRSLAQEAL RDVLSYHIPF LVSSIEDFKD HIPRETDMKV 970 980 990 1000 1010 1020 AMNVYELSSA AGLPCEIDPA LVVALSSQKS ENISPEEEYK IACLLMVFVA VSLPTLASNV 1030 1040 1050 1060 1070 1080 MSQYSPAIEG HCNNIHCLAK AINQIAAALF TIHKGSIEDR LKEFLALASS SLLKIGQETD 1090 1100 1110 1120 1130 KTTTRNRESV YLLLDMIVQE SPFLTMDLLE SCFPYVLLRN AYHAVYKQSV TSSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease." Suzuki T., Nishiyama K., Yamamoto A., Inazawa J., Iwaki T., Yamada T., Kanazawa I., Sakaki Y. Genomics 63:246-254(2000) [PubMed: 10673335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Trachea.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB014509 mRNA. Translation: BAA77295.1. AB011159 mRNA. Translation: BAA25513.2. Different initiation. AK292914 mRNA. Translation: BAF85603.1. BT007033 mRNA. Translation: AAP35681.1. AC108514 Genomic DNA. Translation: AAX93118.1. AC064871 Genomic DNA. Translation: AAY24196.1. BC015025 mRNA. Translation: AAH15025.1.
IPI	IPI00031982. IPI00409684.
RefSeq	NP_038464.1. NP_995314.1.
UniGene	Hs.603732
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y2A7. 7 interactions.
STRING	Q9Y2A7.
PTM databases
PhosphoSite	Q9Y2A7.
Proteomic databases
PRIDE	Q9Y2A7.
Genome annotation databases
Ensembl	ENST00000361354; ENSP00000355348; ENSG00000061676; Homo sapiens. [Genome view]
GeneID	10787.
KEGG	hsa:10787.
UCSC	uc002upc.1. human.
Organism-specific databases
CTD	10787.
GeneCards	GC02M183497.
H-InvDB	HIX0018862.
HGNC	HGNC:7666. NCKAP1.
HPA	HPA020449.
MIM	604891. gene.
PharmGKB	PA31468.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15970.
HOVERGEN	Q9Y2A7.
OMA	MDKLHMA.
PhylomeDB	Q9Y2A7.
Gene expression databases
ArrayExpress	Q9Y2A7.
Bgee	Q9Y2A7.
CleanEx	HS_NCKAP1.
Genevestigator	Q9Y2A7.
GermOnline	ENSG00000061676. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	40972.
SOURCE	Search...

Entry information

Entry name

NCKP1_HUMAN

Accession

Primary (citable) accession number: Q9Y2A7
Secondary accession number(s): O60329 , Q53QN5, Q53S94, Q53Y35

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.