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Protein

Nck-associated protein 1

Gene

NCKAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

GO - Molecular functioni

  • protein complex binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: ProtInc
  • central nervous system development Source: ProtInc
  • Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
  • positive regulation of lamellipodium assembly Source: UniProtKB
  • Rac protein signal transduction Source: UniProtKB
  • vascular endothelial growth factor receptor signaling pathway Source: Reactome
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Enzyme and pathway databases

BioCyciZFISH:ENSG00000061676-MONOMER.
ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 1
Short name:
NAP 1
Alternative name(s):
Membrane-associated protein HEM-2
p125Nap1
Gene namesi
Name:NCKAP1
Synonyms:HEM2, KIAA0587, NAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:7666. NCKAP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei995 – 1015HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lamellipodium membrane Source: UniProtKB-SubCell
  • SCAR complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10787.
OpenTargetsiENSG00000061676.
PharmGKBiPA31468.

Polymorphism and mutation databases

DMDMi12643947.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002161722 – 1128Nck-associated protein 1Add BLAST1127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9Y2A7.
MaxQBiQ9Y2A7.
PaxDbiQ9Y2A7.
PeptideAtlasiQ9Y2A7.
PRIDEiQ9Y2A7.

PTM databases

iPTMnetiQ9Y2A7.
PhosphoSitePlusiQ9Y2A7.
SwissPalmiQ9Y2A7.

Expressioni

Tissue specificityi

Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Gene expression databases

BgeeiENSG00000061676.
CleanExiHS_NCKAP1.
GenevisibleiQ9Y2A7. HS.

Organism-specific databases

HPAiHPA020449.

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Associates preferentially with the first SH3 domain of NCK. Interacts with NYAP1, NYAP2 and MYO16 (By similarity). Interacts with human cytomegalovirus protein UL135.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABI1Q8IZP03EBI-389845,EBI-375446
CYFIP1Q7L5763EBI-389845,EBI-1048143

GO - Molecular functioni

  • protein complex binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116003. 48 interactors.
DIPiDIP-31119N.
IntActiQ9Y2A7. 21 interactors.
MINTiMINT-244824.
STRINGi9606.ENSP00000354251.

Structurei

Secondary structure

11128
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Helixi13 – 39Combined sources27
Turni41 – 43Combined sources3
Helixi46 – 48Combined sources3
Turni51 – 53Combined sources3
Helixi54 – 63Combined sources10
Helixi77 – 81Combined sources5
Helixi83 – 117Combined sources35
Turni123 – 125Combined sources3
Helixi127 – 148Combined sources22
Helixi153 – 165Combined sources13
Beta strandi168 – 170Combined sources3
Helixi175 – 184Combined sources10
Helixi188 – 195Combined sources8
Turni196 – 199Combined sources4
Helixi200 – 208Combined sources9
Helixi209 – 212Combined sources4
Helixi214 – 217Combined sources4
Helixi221 – 226Combined sources6
Turni227 – 230Combined sources4
Beta strandi232 – 235Combined sources4
Helixi236 – 238Combined sources3
Beta strandi239 – 241Combined sources3
Helixi248 – 252Combined sources5
Helixi255 – 268Combined sources14
Helixi271 – 275Combined sources5
Helixi277 – 287Combined sources11
Beta strandi291 – 296Combined sources6
Beta strandi299 – 302Combined sources4
Helixi303 – 311Combined sources9
Helixi315 – 317Combined sources3
Helixi320 – 359Combined sources40
Helixi361 – 364Combined sources4
Turni365 – 367Combined sources3
Helixi368 – 391Combined sources24
Turni397 – 400Combined sources4
Helixi405 – 421Combined sources17
Helixi423 – 436Combined sources14
Helixi438 – 446Combined sources9
Helixi454 – 468Combined sources15
Helixi472 – 476Combined sources5
Helixi484 – 498Combined sources15
Helixi506 – 508Combined sources3
Helixi510 – 527Combined sources18
Helixi529 – 537Combined sources9
Helixi540 – 544Combined sources5
Helixi546 – 558Combined sources13
Helixi560 – 563Combined sources4
Helixi564 – 567Combined sources4
Helixi568 – 572Combined sources5
Helixi573 – 579Combined sources7
Helixi585 – 587Combined sources3
Helixi588 – 626Combined sources39
Helixi630 – 633Combined sources4
Helixi634 – 640Combined sources7
Helixi662 – 664Combined sources3
Helixi674 – 689Combined sources16
Beta strandi695 – 697Combined sources3
Beta strandi700 – 702Combined sources3
Helixi704 – 723Combined sources20
Turni728 – 731Combined sources4
Helixi736 – 752Combined sources17
Helixi753 – 755Combined sources3
Helixi761 – 771Combined sources11
Beta strandi774 – 776Combined sources3
Helixi784 – 794Combined sources11
Helixi796 – 801Combined sources6
Beta strandi804 – 808Combined sources5
Turni809 – 812Combined sources4
Beta strandi813 – 816Combined sources4
Beta strandi818 – 820Combined sources3
Helixi828 – 831Combined sources4
Helixi833 – 871Combined sources39
Helixi874 – 882Combined sources9
Helixi887 – 894Combined sources8
Helixi900 – 931Combined sources32
Helixi933 – 944Combined sources12
Helixi955 – 964Combined sources10
Helixi973 – 979Combined sources7
Helixi989 – 1006Combined sources18
Helixi1007 – 1012Combined sources6
Helixi1014 – 1016Combined sources3
Turni1020 – 1023Combined sources4
Helixi1029 – 1031Combined sources3
Helixi1032 – 1046Combined sources15
Helixi1051 – 1068Combined sources18
Helixi1069 – 1071Combined sources3
Turni1075 – 1080Combined sources6
Helixi1081 – 1094Combined sources14
Helixi1100 – 1106Combined sources7
Helixi1109 – 1120Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P8CX-ray2.29B1-1128[»]
4N78X-ray2.43B1-1128[»]
ProteinModelPortaliQ9Y2A7.
SMRiQ9Y2A7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y2A7.

Family & Domainsi

Sequence similaritiesi

Belongs to the HEM-1/HEM-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1917. Eukaryota.
ENOG410XPQI. LUCA.
GeneTreeiENSGT00390000016619.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiQ9Y2A7.
KOiK05750.
OMAiYNAAYEL.
OrthoDBiEOG091G03SF.
PhylomeDBiQ9Y2A7.
TreeFamiTF313683.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2A7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID
60 70 80 90 100
KNLESAVKFI VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV
110 120 130 140 150
MEFKDHVCEL LNTIDVCQVF FDITVNFDLT KNYLDLIITY TTLMILLSRI
160 170 180 190 200
EERKAIIGLY NYAHEMTHGA SDREYPRLGQ MIVDYENPLK KMMEEFVPHS
210 220 230 240 250
KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML NPAQSDTMPC
260 270 280 290 300
EYLSLDAMEK WIIFGFILCH GILNTDATAL NLWKLALQSS SCLSLFRDEV
310 320 330 340 350
FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL
360 370 380 390 400
KELATVLSDQ PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF
410 420 430 440 450
IDKHIAELIF YMEELRAHVR KYGPVMQRYY VQYLSGFDAV VLNELVQNLS
460 470 480 490 500
VCPEDESIIM SSFVNTMTSL SVKQVEDGEV FDFRGMRLDW FRLQAYTSVS
510 520 530 540 550
KASLGLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS IFCFYSRAFE
560 570 580 590 600
KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN
610 620 630 640 650
MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG
660 670 680 690 700
KKGEPEREKP GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMVVWEH
710 720 730 740 750
TFTPREYLTS HLEIRFTKSI VGMTMYNQAT QEIAKPSELL TSVRAYMTVL
760 770 780 790 800
QSIENYVQID ITRVFNNVLL QQTQHLDSHG EPTITSLYTN WYLETLLRQV
810 820 830 840 850
SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS ELLGPYGMKF
860 870 880 890 900
LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV
910 920 930 940 950
DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET
960 970 980 990 1000
DMKVAMNVYE LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM
1010 1020 1030 1040 1050
VFVAVSLPTL ASNVMSQYSP AIEGHCNNIH CLAKAINQIA AALFTIHKGS
1060 1070 1080 1090 1100
IEDRLKEFLA LASSSLLKIG QETDKTTTRN RESVYLLLDM IVQESPFLTM
1110 1120
DLLESCFPYV LLRNAYHAVY KQSVTSSA
Length:1,128
Mass (Da):128,790
Last modified:November 1, 1999 - v1
Checksum:i12D32BAC7080CB5A
GO
Isoform 2 (identifier: Q9Y2A7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-36: K → KQGQVWK

Show »
Length:1,134
Mass (Da):129,517
Checksum:i71019B9B0BCA760D
GO

Sequence cautioni

The sequence BAA25513 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03655836K → KQGQVWK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014509 mRNA. Translation: BAA77295.1.
AB011159 mRNA. Translation: BAA25513.2. Different initiation.
AK292914 mRNA. Translation: BAF85603.1.
BT007033 mRNA. Translation: AAP35681.1.
AC108514 Genomic DNA. Translation: AAX93118.1.
AC064871 Genomic DNA. Translation: AAY24196.1.
BC015025 mRNA. Translation: AAH15025.1.
CCDSiCCDS2287.1. [Q9Y2A7-1]
CCDS2288.1. [Q9Y2A7-2]
RefSeqiNP_038464.1. NM_013436.4. [Q9Y2A7-1]
NP_995314.1. NM_205842.2. [Q9Y2A7-2]
UniGeneiHs.603732.
Hs.708541.

Genome annotation databases

EnsembliENST00000360982; ENSP00000354251; ENSG00000061676. [Q9Y2A7-2]
ENST00000361354; ENSP00000355348; ENSG00000061676. [Q9Y2A7-1]
GeneIDi10787.
KEGGihsa:10787.
UCSCiuc002upb.5. human. [Q9Y2A7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014509 mRNA. Translation: BAA77295.1.
AB011159 mRNA. Translation: BAA25513.2. Different initiation.
AK292914 mRNA. Translation: BAF85603.1.
BT007033 mRNA. Translation: AAP35681.1.
AC108514 Genomic DNA. Translation: AAX93118.1.
AC064871 Genomic DNA. Translation: AAY24196.1.
BC015025 mRNA. Translation: AAH15025.1.
CCDSiCCDS2287.1. [Q9Y2A7-1]
CCDS2288.1. [Q9Y2A7-2]
RefSeqiNP_038464.1. NM_013436.4. [Q9Y2A7-1]
NP_995314.1. NM_205842.2. [Q9Y2A7-2]
UniGeneiHs.603732.
Hs.708541.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P8CX-ray2.29B1-1128[»]
4N78X-ray2.43B1-1128[»]
ProteinModelPortaliQ9Y2A7.
SMRiQ9Y2A7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116003. 48 interactors.
DIPiDIP-31119N.
IntActiQ9Y2A7. 21 interactors.
MINTiMINT-244824.
STRINGi9606.ENSP00000354251.

PTM databases

iPTMnetiQ9Y2A7.
PhosphoSitePlusiQ9Y2A7.
SwissPalmiQ9Y2A7.

Polymorphism and mutation databases

DMDMi12643947.

Proteomic databases

EPDiQ9Y2A7.
MaxQBiQ9Y2A7.
PaxDbiQ9Y2A7.
PeptideAtlasiQ9Y2A7.
PRIDEiQ9Y2A7.

Protocols and materials databases

DNASUi10787.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360982; ENSP00000354251; ENSG00000061676. [Q9Y2A7-2]
ENST00000361354; ENSP00000355348; ENSG00000061676. [Q9Y2A7-1]
GeneIDi10787.
KEGGihsa:10787.
UCSCiuc002upb.5. human. [Q9Y2A7-1]

Organism-specific databases

CTDi10787.
DisGeNETi10787.
GeneCardsiNCKAP1.
HGNCiHGNC:7666. NCKAP1.
HPAiHPA020449.
MIMi604891. gene.
neXtProtiNX_Q9Y2A7.
OpenTargetsiENSG00000061676.
PharmGKBiPA31468.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1917. Eukaryota.
ENOG410XPQI. LUCA.
GeneTreeiENSGT00390000016619.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiQ9Y2A7.
KOiK05750.
OMAiYNAAYEL.
OrthoDBiEOG091G03SF.
PhylomeDBiQ9Y2A7.
TreeFamiTF313683.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000061676-MONOMER.
ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

ChiTaRSiNCKAP1. human.
EvolutionaryTraceiQ9Y2A7.
GeneWikiiNCKAP1.
GenomeRNAii10787.
PROiQ9Y2A7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000061676.
CleanExiHS_NCKAP1.
GenevisibleiQ9Y2A7. HS.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCKP1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2A7
Secondary accession number(s): O60329
, Q53QN5, Q53S94, Q53Y35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.