Q9Y295 (DRG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Developmentally-regulated GTP-binding protein 1
Short name=DRG-1

Alternative name(s):
Neural precursor cell expressed developmentally down-regulated protein 3
Short name=NEDD-3

Gene names

Name:	DRG1
Synonyms:	NEDD3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Critical regulator of cell growth under specific conditions. Implicated in differentiation and cell cycle arrest.
Subunit structure	Interacts (via its C-terminal) with TAL1. Interacts with ZC3H15; the interaction forms a polysomal DRG1-DFRP1/ZC3H15 complex which provides protein stability to DRG1 possibly by blocking poly-ubiquitination. Interacts with STK16; the interaction phosphorylates STK16 By similarity. Ref.1 Ref.9 Ref.12
Subcellular location	Cytoplasm. Note: The DRG1-DFRP2/ZC3H15 complex associates with polysomes. Ref.9 Ref.12
Tissue specificity	High levels in skeletal muscle, heart, and kidney. Intermediate levels in liver, placenta and brain. Low levels in colon, thymus, spleen, small intestine, lung and leukocytes. Ref.2
Induction	By androgens.
Post-translational modification	Sumoylated by UBE2I in response to MEKK1-mediated stimuli. Ref.10
Sequence similarities	Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Traceable author statement PubMed 1280421. Source: ProtInc transcription, DNA-dependent Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA intermediate filament cytoskeleton Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA polysome Inferred from direct assay Ref.12. Source: UniProtKB
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW transcription factor binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RWDD1	Q9H446	2	EBI-719554,EBI-748952

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

Developmentally-regulated GTP-binding protein 1

PRO_0000205424

Regions

Domain

76 – 169

94

G

Nucleotide binding

71 – 78

8

GTP By similarity

Nucleotide binding

117 – 121

5

GTP By similarity

Nucleotide binding

248 – 251

4

GTP By similarity

Region

1 – 16

16

Required for interaction with STK16

Compositional bias

47 – 56

10

Poly-Gly

Amino acid modifications

Modified residue

100

1

Phosphothreonine; by STK16 Ref.11

Experimental info

Sequence conflict

91

1

V → A in AAH20803. Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

367

Helix Strand Turn

Details...

Beta strand

291 – 296

6

Beta strand

305 – 307

3

Beta strand

309 – 312

4

Helix

318 – 325

8

Turn

330 – 332

3

Beta strand

333 – 340

8

Beta strand

343 – 350

8

Beta strand

358 – 362

5

Beta strand

364 – 366

3

Sequences

	Sequence		Length	Mass (Da)
	Q9Y295 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4A177EA7C8E23005	FASTA	367	40,542
	10 20 30 40 50 60 MSSTLAKIAE IEAEMARTQK NKATAHHLGL LKARLAKLRR ELITPKGGGG GGPGEGFDVA 70 80 90 100 110 120 KTGDARIGFV GFPSVGKSTL LSNLAGVYSE VAAYEFTTLT TVPGVIRYKG AKIQLLDLPG 130 140 150 160 170 180 IIEGAKDGKG RGRQVIAVAR TCNLILIVLD VLKPLGHKKI IENELEGFGI RLNSKPPNIG 190 200 210 220 230 240 FKKKDKGGIN LTATCPQSEL DAETVKSILA EYKIHNADVT LRSDATADDL IDVVEGNRVY 250 260 270 280 290 300 IPCIYVLNKI DQISIEELDI IYKVPHCVPI SAHHRWNFDD LLEKIWDYLK LVRIYTKPKG 310 320 330 340 350 360 QLPDYTSPVV LPYSRTTVED FCMKIHKNLI KEFKYALVWG LSVKHNPQKV GKDHTLEDED VIQIVKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SCL binds the human homologue of DRG in vivo." Zhao X.-F., Aplan P.D. Biochim. Biophys. Acta 1448:109-114(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TAL1.
[2]	"DRG represents a family of two closely related GTP-binding proteins." Li B., Trueeb B. Biochim. Biophys. Acta 1491:196-204(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Prostate.
[9]	"Identification of DRG family regulatory proteins (DFRPs): specific regulation of DRG1 and DRG2." Ishikawa K., Azuma S., Ikawa S., Semba K., Inoue J. Genes Cells 10:139-150(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZC3H15, SUBCELLULAR LOCATION.
[10]	"Ubc9 fusion-directed SUMOylation identifies constitutive and inducible SUMOylation." Jakobs A., Himstedt F., Funk M., Korn B., Gaestel M., Niedenthal R. Nucleic Acids Res. 35:E109-E109(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUMOYLATION.
[11]	"Structure of the human protein kinase MPSK1 reveals an atypical activation loop architecture." Eswaran J., Bernad A., Ligos J.M., Guinea B., Debreczeni J.E., Sobott F., Parker S.A., Najmanovich R., Turk B.E., Knapp S. Structure 16:115-124(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-100, MASS SPECTROMETRY.
[12]	"Independent stabilizations of polysomal Drg1/Dfrp1 complex and non-polysomal Drg2/Dfrp2 complex in mammalian cells." Ishikawa K., Akiyama T., Ito K., Semba K., Inoue J. Biochem. Biophys. Res. Commun. 390:552-556(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZC3H15 IN THE DRG1-DFRP1/ZC3H15 COMPLEX, SUBCELLULAR LOCATION.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"Solution structures of the TGS domain of human developmentally-regulated GTP-binding protein 1." RIKEN structural genomics initiative (RSGI) Submitted (SEP-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 288-367.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF078103 mRNA. Translation: AAD12240.1.
AJ005940 mRNA. Translation: CAA06775.1.
CR542059 mRNA. Translation: CAG46856.1.
CR456488 mRNA. Translation: CAG30374.1.
BT007237 mRNA. Translation: AAP35901.1.
AK315659 mRNA. Translation: BAG38025.1.
AL096701, AL096702 Genomic DNA. Translation: CAI12876.1.
AL096702, AL096701 Genomic DNA. Translation: CAH71744.1.
CH471095 Genomic DNA. Translation: EAW59976.1.
BC019285 mRNA. Translation: AAH19285.1.
BC020803 mRNA. Translation: AAH20803.1.

IPI

IPI00031836.

RefSeq

NP_004138.1. NM_004147.3.

UniGene

Hs.115242.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EKI	NMR	-	A	288-367	[»]

ProteinModelPortal

Q9Y295.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y295. 6 interactions.

MINT

MINT-5006718.

STRING

9606.ENSP00000329715.

PTM databases

PhosphoSite

Q9Y295.

Polymorphism databases

DMDM

6685390.

Proteomic databases

PaxDb

Q9Y295.

PeptideAtlas

Q9Y295.

PRIDE

Q9Y295.

Protocols and materials databases

DNASU

4733.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000331457; ENSP00000329715; ENSG00000185721.

GeneID

4733.

KEGG

hsa:4733.

UCSC

uc003aku.3. human.

Organism-specific databases

CTD

4733.

GeneCards

GC22P031795.

HGNC

HGNC:3029. DRG1.

HPA

HPA001218.

MIM

603952. gene.

neXtProt

NX_Q9Y295.

PharmGKB

PA27483.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1163.

HOGENOM

HOG000112078.

HOVERGEN

HBG000948.

InParanoid

Q9Y295.

OMA

FDVARTG.

OrthoDB

EOG4VHK6R.

PhylomeDB

Q9Y295.

Gene expression databases

ArrayExpress

Q9Y295.

Bgee

Q9Y295.

CleanEx

HS_DRG1.

Genevestigator

Q9Y295.

GermOnline

ENSG00000185721. Homo sapiens.

Family and domain databases

InterPro

IPR006074. GTP1-OBG_CS.
IPR006073. GTP_binding_domain.
IPR005225. Small_GTP-bd_dom.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]

Pfam

PF01926. MMR_HSR1. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]

PRINTS

PR00326. GTP1OBG.

SUPFAM

SSF81271. TGS-like. 1 hit.

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS00905. GTP1_OBG. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

DRG1. human.

EvolutionaryTrace

Q9Y295.

GenomeRNAi

4733.

NextBio

18246.

SOURCE

Search...

Entry information

Entry name

DRG1_HUMAN

Accession

Primary (citable) accession number: Q9Y295
Secondary accession number(s): B2RDS8 , Q6FGP8, Q8WW69, Q9UGF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.