##gff-version 3 Q9Y289 UniProtKB Chain 1 635 . . . ID=PRO_0000105386;Note=Sodium-dependent multivitamin transporter Q9Y289 UniProtKB Transmembrane 24 44 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 68 88 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 101 121 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 143 163 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 176 196 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 199 219 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 256 276 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 297 317 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 336 356 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 396 416 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 428 448 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 456 476 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Transmembrane 528 548 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Glycosylation 138 138 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Glycosylation 489 489 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Glycosylation 498 498 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255 Q9Y289 UniProtKB Natural variant 94 635 . . . ID=VAR_084535;Note=In SMVTD and COMNB%3B reduced membrane localization%3B impaired biotin transport. Missing;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:27904971,ECO:0000269|PubMed:35013551;Dbxref=dbSNP:rs994218778,PMID:27904971,PMID:35013551 Q9Y289 UniProtKB Natural variant 123 123 . . . ID=VAR_084536;Note=In SMVTD%3B reduced membrane localization%3B impaired biotin transport. R->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27904971;Dbxref=PMID:27904971 Q9Y289 UniProtKB Natural variant 162 162 . . . ID=VAR_087446;Note=In COMNB%3B no effect on membrane localization. Y->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:35013551;Dbxref=PMID:35013551 Q9Y289 UniProtKB Natural variant 400 400 . . . ID=VAR_084537;Note=In SMVTD%3B impaired biotin transport. R->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31754459;Dbxref=dbSNP:rs370950187,PMID:31754459 Q9Y289 UniProtKB Natural variant 429 429 . . . ID=VAR_087447;Note=In COMNB%3B no effect on membrane localization. S->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:35013551;Dbxref=PMID:35013551 Q9Y289 UniProtKB Natural variant 481 481 . . . ID=VAR_052491;Note=S->F;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10329687,ECO:0000269|PubMed:10334869,ECO:0000269|PubMed:14702039,ECO:0000269|Ref.5;Dbxref=dbSNP:rs1395,PMID:10329687,PMID:10334869,PMID:14702039 Q9Y289 UniProtKB Natural variant 492 492 . . . ID=VAR_052492;Note=S->N;Dbxref=dbSNP:rs1064845 Q9Y289 UniProtKB Mutagenesis 68 68 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 78 78 . . . Note=Reduced membrane localization. Decrease in biotin transport. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28052864;Dbxref=PMID:28052864 Q9Y289 UniProtKB Mutagenesis 104 104 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 128 128 . . . Note=No effect on biotin transport. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28052864;Dbxref=PMID:28052864 Q9Y289 UniProtKB Mutagenesis 138 138 . . . Note=Reduced protein levels. Decrease in biotin transport. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21570947;Dbxref=PMID:21570947 Q9Y289 UniProtKB Mutagenesis 144 144 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 187 187 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 242 242 . . . Note=No effect on biotin transport. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28052864;Dbxref=PMID:28052864 Q9Y289 UniProtKB Mutagenesis 283 283 . . . Note=No effect on protein levels or membrane localization. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21570947;Dbxref=PMID:21570947 Q9Y289 UniProtKB Mutagenesis 286 286 . . . Note=Resistant to phorbol 12-myristate 13-acetate (PMA)-induced inhibition of biotin transport. No effect on protein levels or membrane localization. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21570947;Dbxref=PMID:21570947 Q9Y289 UniProtKB Mutagenesis 294 294 . . . Note=Reduced membrane localization. Decrease in biotin transport (decreased Vmax%2C no change in Km). C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 294 294 . . . Note=Decrease in biotin transport. C->S%2CM;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 309 309 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 358 358 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 366 366 . . . Note=No effect on biotin transport. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28052864;Dbxref=PMID:28052864 Q9Y289 UniProtKB Mutagenesis 410 410 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 443 443 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 450 450 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 489 489 . . . Note=Slight decrease in protein levels. Decrease in biotin transport. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21570947;Dbxref=PMID:21570947 Q9Y289 UniProtKB Mutagenesis 498 498 . . . Note=No effect on biotin transport. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21570947;Dbxref=PMID:21570947 Q9Y289 UniProtKB Mutagenesis 534 534 . . . Note=No effect on biotin transport. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21570947;Dbxref=PMID:21570947 Q9Y289 UniProtKB Mutagenesis 567 635 . . . Note=Loss of biotin transport. Loss of membrane localization. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 570 635 . . . Note=Loss of biotin transport. Loss of membrane localization. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 575 635 . . . Note=Partial loss (75%25) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 577 577 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 583 583 . . . Note=No effect on biotin transport. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 584 635 . . . Note=Partial loss (75%25) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 600 635 . . . Note=Partial loss (75%25) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 612 635 . . . Note=Partial loss (75%25) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 616 635 . . . Note=Loss of apical membrane localization in polarized cells. Basolateral localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 620 635 . . . Note=Partial loss (25%25) of biotine transport. No change in apical membrane localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 624 635 . . . Note=Partial loss (25%25) of biotine transport. No change in apical membrane localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916 Q9Y289 UniProtKB Mutagenesis 627 627 . . . Note=No effect on biotin transport. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28052864;Dbxref=PMID:28052864 Q9Y289 UniProtKB Mutagenesis 628 628 . . . Note=Decrease in biotin transport. C->A%2CS;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22015582;Dbxref=PMID:22015582 Q9Y289 UniProtKB Mutagenesis 632 635 . . . Note=Partial loss (25%25) of biotine transport. No change in apical membrane localization in polarized cells. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19211916;Dbxref=PMID:19211916