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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

FARSA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei329L-phenylalanineCombined sources1 Publication1
Binding sitei435L-phenylalanineCombined sources1 Publication1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phenylalanine-tRNA ligase activity Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • tRNA binding Source: InterPro

GO - Biological processi

  • phenylalanyl-tRNA aminoacylation Source: UniProtKB
  • protein heterotetramerization Source: UniProtKB
  • tRNA aminoacylation for protein translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00249-MONOMER.
BRENDAi6.1.1.20. 2681.
ReactomeiR-HSA-379716. Cytosolic tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.201 Publication)
Alternative name(s):
CML33
Phenylalanyl-tRNA synthetase alpha subunit
Short name:
PheRS
Gene namesi
Name:FARSA
Synonyms:FARS, FARSL, FARSLA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:3592. FARSA.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • cytosol Source: Reactome
  • membrane Source: UniProtKB
  • phenylalanine-tRNA ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2193.
OpenTargetsiENSG00000179115.
PharmGKBiPA28005.

Chemistry databases

DrugBankiDB00120. L-Phenylalanine.

Polymorphism and mutation databases

BioMutaiFARSA.
DMDMi12643946.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001268242 – 508Phenylalanine--tRNA ligase alpha subunitAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei190PhosphothreonineCombined sources1
Modified residuei193PhosphoserineCombined sources1
Modified residuei301PhosphoserineBy similarity1
Modified residuei311N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y285.
PaxDbiQ9Y285.
PeptideAtlasiQ9Y285.
PRIDEiQ9Y285.

PTM databases

iPTMnetiQ9Y285.
PhosphoSitePlusiQ9Y285.

Expressioni

Gene expression databases

BgeeiENSG00000179115.
CleanExiHS_FARSA.
ExpressionAtlasiQ9Y285. baseline and differential.
GenevisibleiQ9Y285. HS.

Organism-specific databases

HPAiHPA001911.

Interactioni

Subunit structurei

Heterotetramer; dimer of two heterodimers formed by FARSA and FARSB.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABI3Q9P2A43EBI-725361,EBI-742038
ECSITQ9BQ952EBI-725361,EBI-712452

Protein-protein interaction databases

BioGridi108487. 38 interactors.
DIPiDIP-53610N.
IntActiQ9Y285. 24 interactors.
MINTiMINT-1378519.
STRINGi9606.ENSP00000320309.

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13
Beta strandi17 – 19Combined sources3
Helixi25 – 28Combined sources4
Turni29 – 31Combined sources3
Helixi37 – 41Combined sources5
Helixi43 – 46Combined sources4
Beta strandi53 – 61Combined sources9
Helixi65 – 73Combined sources9
Helixi76 – 83Combined sources8
Helixi91 – 94Combined sources4
Helixi98 – 108Combined sources11
Turni109 – 111Combined sources3
Beta strandi112 – 114Combined sources3
Beta strandi119 – 121Combined sources3
Beta strandi126 – 128Combined sources3
Helixi135 – 143Combined sources9
Turni144 – 149Combined sources6
Helixi153 – 161Combined sources9
Beta strandi167 – 175Combined sources9
Turni194 – 198Combined sources5
Turni201 – 204Combined sources4
Beta strandi212 – 214Combined sources3
Helixi226 – 240Combined sources15
Beta strandi251 – 254Combined sources4
Helixi255 – 258Combined sources4
Helixi260 – 262Combined sources3
Beta strandi266 – 268Combined sources3
Turni273 – 275Combined sources3
Beta strandi278 – 280Combined sources3
Helixi290 – 301Combined sources12
Helixi316 – 319Combined sources4
Beta strandi321 – 324Combined sources4
Helixi329 – 340Combined sources12
Beta strandi342 – 344Combined sources3
Beta strandi348 – 357Combined sources10
Beta strandi364 – 366Combined sources3
Beta strandi368 – 381Combined sources14
Helixi384 – 396Combined sources13
Turni397 – 399Combined sources3
Beta strandi404 – 407Combined sources4
Beta strandi414 – 421Combined sources8
Beta strandi430 – 436Combined sources7
Helixi440 – 443Combined sources4
Helixi444 – 446Combined sources3
Beta strandi452 – 461Combined sources10
Helixi462 – 465Combined sources4
Turni466 – 470Combined sources5
Helixi474 – 476Combined sources3
Helixi484 – 489Combined sources6
Turni497 – 499Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L4GX-ray3.30A/C/E/G/I/K/M/O1-508[»]
ProteinModelPortaliQ9Y285.
SMRiQ9Y285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni372 – 374L-phenylalanine bindingCombined sources1 Publication3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2784. Eukaryota.
COG0016. LUCA.
GeneTreeiENSGT00390000006387.
HOGENOMiHOG000230294.
HOVERGENiHBG068046.
InParanoidiQ9Y285.
KOiK01889.
PhylomeDBiQ9Y285.
TreeFamiTF300647.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y285-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADGQVAELL LRRLEASDGG LDSAELAAEL GMEHQAVVGA VKSLQALGEV
60 70 80 90 100
IEAELRSTKH WELTAEGEEI AREGSHEARV FRSIPPEGLA QSELMRLPSG
110 120 130 140 150
KVGFSKAMSN KWIRVDKSAA DGPRVFRVVD SMEDEVQRRL QLVRGGQAEK
160 170 180 190 200
LGEKERSELR KRKLLAEVTL KTYWVSKGSA FSTSISKQET ELSPEMISSG
210 220 230 240 250
SWRDRPFKPY NFLAHGVLPD SGHLHPLLKV RSQFRQIFLE MGFTEMPTDN
260 270 280 290 300
FIESSFWNFD ALFQPQQHPA RDQHDTFFLR DPAEALQLPM DYVQRVKRTH
310 320 330 340 350
SQGGYGSQGY KYNWKLDEAR KNLLRTHTTS ASARALYRLA QKKPFTPVKY
360 370 380 390 400
FSIDRVFRNE TLDATHLAEF HQIEGVVADH GLTLGHLMGV LREFFTKLGI
410 420 430 440 450
TQLRFKPAYN PYTEPSMEVF SYHQGLKKWV EVGNSGVFRP EMLLPMGLPE
460 470 480 490 500
NVSVIAWGLS LERPTMIKYG INNIRELVGH KVNLQMVYDS PLCRLDAEPR

PPPTQEAA
Length:508
Mass (Da):57,564
Last modified:January 23, 2007 - v3
Checksum:i99BC12E1F3C5FCFD
GO
Isoform 2 (identifier: Q9Y285-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-199: Missing.

Note: No experimental confirmation available.
Show »
Length:477
Mass (Da):54,158
Checksum:i156A50E73126F137
GO

Sequence cautioni

The sequence AAB51175 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179S → G in BAA95666 (Ref. 3) Curated1
Sequence conflicti376V → L in BAA95666 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052641341Q → R.Corresponds to variant rs35087277dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056196169 – 199Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07424 mRNA. Translation: AAB61694.1.
AF042347 mRNA. Translation: AAD02221.1.
D84471 mRNA. Translation: BAA95666.1.
BT007198 mRNA. Translation: AAP35862.1.
AK304587 mRNA. Translation: BAG65375.1.
AD000092 Genomic DNA. Translation: AAB51175.1. Sequence problems.
BC006495 mRNA. Translation: AAH06495.1.
BC043565 mRNA. Translation: AAH43565.1.
CCDSiCCDS12287.1. [Q9Y285-1]
PIRiT45074.
RefSeqiNP_004452.1. NM_004461.2. [Q9Y285-1]
UniGeneiHs.23111.

Genome annotation databases

EnsembliENST00000314606; ENSP00000320309; ENSG00000179115. [Q9Y285-1]
ENST00000423140; ENSP00000396548; ENSG00000179115. [Q9Y285-2]
GeneIDi2193.
KEGGihsa:2193.
UCSCiuc002mvs.3. human. [Q9Y285-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07424 mRNA. Translation: AAB61694.1.
AF042347 mRNA. Translation: AAD02221.1.
D84471 mRNA. Translation: BAA95666.1.
BT007198 mRNA. Translation: AAP35862.1.
AK304587 mRNA. Translation: BAG65375.1.
AD000092 Genomic DNA. Translation: AAB51175.1. Sequence problems.
BC006495 mRNA. Translation: AAH06495.1.
BC043565 mRNA. Translation: AAH43565.1.
CCDSiCCDS12287.1. [Q9Y285-1]
PIRiT45074.
RefSeqiNP_004452.1. NM_004461.2. [Q9Y285-1]
UniGeneiHs.23111.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L4GX-ray3.30A/C/E/G/I/K/M/O1-508[»]
ProteinModelPortaliQ9Y285.
SMRiQ9Y285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108487. 38 interactors.
DIPiDIP-53610N.
IntActiQ9Y285. 24 interactors.
MINTiMINT-1378519.
STRINGi9606.ENSP00000320309.

Chemistry databases

DrugBankiDB00120. L-Phenylalanine.

PTM databases

iPTMnetiQ9Y285.
PhosphoSitePlusiQ9Y285.

Polymorphism and mutation databases

BioMutaiFARSA.
DMDMi12643946.

Proteomic databases

EPDiQ9Y285.
PaxDbiQ9Y285.
PeptideAtlasiQ9Y285.
PRIDEiQ9Y285.

Protocols and materials databases

DNASUi2193.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314606; ENSP00000320309; ENSG00000179115. [Q9Y285-1]
ENST00000423140; ENSP00000396548; ENSG00000179115. [Q9Y285-2]
GeneIDi2193.
KEGGihsa:2193.
UCSCiuc002mvs.3. human. [Q9Y285-1]

Organism-specific databases

CTDi2193.
DisGeNETi2193.
GeneCardsiFARSA.
HGNCiHGNC:3592. FARSA.
HPAiHPA001911.
MIMi602918. gene.
neXtProtiNX_Q9Y285.
OpenTargetsiENSG00000179115.
PharmGKBiPA28005.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2784. Eukaryota.
COG0016. LUCA.
GeneTreeiENSGT00390000006387.
HOGENOMiHOG000230294.
HOVERGENiHBG068046.
InParanoidiQ9Y285.
KOiK01889.
PhylomeDBiQ9Y285.
TreeFamiTF300647.

Enzyme and pathway databases

BioCyciZFISH:HS00249-MONOMER.
BRENDAi6.1.1.20. 2681.
ReactomeiR-HSA-379716. Cytosolic tRNA aminoacylation.

Miscellaneous databases

GeneWikiiFARSA_(gene).
GenomeRNAii2193.
PROiQ9Y285.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000179115.
CleanExiHS_FARSA.
ExpressionAtlasiQ9Y285. baseline and differential.
GenevisibleiQ9Y285. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFA_HUMAN
AccessioniPrimary (citable) accession number: Q9Y285
Secondary accession number(s): B4E363, Q9NSD8, Q9Y4W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 168 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.