Q9Y282 (ERGI3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoplasmic reticulum-Golgi intermediate compartment protein 3

Alternative name(s):
Serologically defined breast cancer antigen NY-BR-84

Gene names

Name:	ERGIC3
Synonyms:	C20orf47, ERV46, SDBCAG84
ORF Names:	CGI-54, PRO0989

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in transport between endoplasmic reticulum and Golgi By similarity.
Subunit structure	Interacts with ERGIC1/ERGIC32. Ref.8
Subcellular location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus › cis-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Cycles between the endoplasmic reticulum and the Golgi. Ref.8
Sequence similarities	Belongs to the ERGIC family.
Sequence caution	The sequence AAF71038.1 differs from that shown. Reason: Erroneous initiation. The sequence AAG48265.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum-Golgi intermediate compartment membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERGIC1	Q969X5	1	EBI-781551,EBI-781527

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y282-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y282-2)

The sequence of this isoform differs from the canonical sequence as follows:
     229-230: VH → GE
     231-383: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9Y282-3)

The sequence of this isoform differs from the canonical sequence as follows:
     229-229: V → VHAVEI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Endoplasmic reticulum-Golgi intermediate compartment protein 3		PRO_0000097640
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Helical; Potential
	Topological domain	47 – 341	295	Lumenal Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Topological domain	363 – 383	21	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	241	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	229 – 230	2	VH → GE in isoform 2.		VSP_008652
	Alternative sequence	229	1	V → VHAVEI in isoform 3.		VSP_019208
	Alternative sequence	231 – 383	153	Missing in isoform 2.		VSP_008653
	Natural variant	113	1	I → L. Corresponds to variant rs35505616 [ dbSNP | Ensembl ].		VAR_048939
	Natural variant	297	1	T → K in a colorectal cancer sample; somatic mutation. Ref.11		VAR_036553
Experimental info
	Sequence conflict	239	1	N → D in BAC11054. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8370FB3067AD95CE	FASTA	383	43,222
	10 20 30 40 50 60 MEALGKLKQF DAYPKTLEDF RVKTCGGATV TIVSGLLMLL LFLSELQYYL TTEVHPELYV 70 80 90 100 110 120 DKSRGDKLKI NIDVLFPHMP CAYLSIDAMD VAGEQQLDVE HNLFKQRLDK DGIPVSSEAE 130 140 150 160 170 180 RHELGKVEVT VFDPDSLDPD RCESCYGAEA EDIKCCNTCE DVREAYRRRG WAFKNPDTIE 190 200 210 220 230 240 QCRREGFSQK MQEQKNEGCQ VYGFLEVNKV AGNFHFAPGK SFQQSHVHVH DLQSFGLDNI 250 260 270 280 290 300 NMTHYIQHLS FGEDYPGIVN PLDHTNVTAP QASMMFQYFV KVVPTVYMKV DGEVLRTNQF 310 320 330 340 350 360 SVTRHEKVAN GLLGDQGLPG VFVLYELSPM MVKLTEKHRS FTHFLTGVCA IIGGMFTVAG 370 380 LIDSLIYHSA RAIQKKIDLG KTT
	Isoform 2 [UniParc]. Checksum: 11727EE72A9A2587	FASTA	230	26,155
	10 20 30 40 50 60 MEALGKLKQF DAYPKTLEDF RVKTCGGATV TIVSGLLMLL LFLSELQYYL TTEVHPELYV 70 80 90 100 110 120 DKSRGDKLKI NIDVLFPHMP CAYLSIDAMD VAGEQQLDVE HNLFKQRLDK DGIPVSSEAE 130 140 150 160 170 180 RHELGKVEVT VFDPDSLDPD RCESCYGAEA EDIKCCNTCE DVREAYRRRG WAFKNPDTIE 190 200 210 220 230 QCRREGFSQK MQEQKNEGCQ VYGFLEVNKV AGNFHFAPGK SFQQSHVHGE
	Isoform 3 [UniParc]. Checksum: 54AE001D4A66DAF4	FASTA	388	43,772
	10 20 30 40 50 60 MEALGKLKQF DAYPKTLEDF RVKTCGGATV TIVSGLLMLL LFLSELQYYL TTEVHPELYV 70 80 90 100 110 120 DKSRGDKLKI NIDVLFPHMP CAYLSIDAMD VAGEQQLDVE HNLFKQRLDK DGIPVSSEAE 130 140 150 160 170 180 RHELGKVEVT VFDPDSLDPD RCESCYGAEA EDIKCCNTCE DVREAYRRRG WAFKNPDTIE 190 200 210 220 230 240 QCRREGFSQK MQEQKNEGCQ VYGFLEVNKV AGNFHFAPGK SFQQSHVHVH AVEIHDLQSF 250 260 270 280 290 300 GLDNINMTHY IQHLSFGEDY PGIVNPLDHT NVTAPQASMM FQYFVKVVPT VYMKVDGEVL 310 320 330 340 350 360 RTNQFSVTRH EKVANGLLGD QGLPGVFVLY ELSPMMVKLT EKHRSFTHFL TGVCAIIGGM 370 380 FTVAGLIDSL IYHSARAIQK KIDLGKTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression." Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J. Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Mammary gland.
[2]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Embryo.
[4]	"Human hypothetical 43.2 Kd protein." Zhang J., Liu T., Ye M., Zhang Q., Fu G., Zhou J., Wu J., Shen Y., Yu M., Chen S., Mao M., Chen Z. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Skin.
[7]	"Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver." Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-383 (ISOFORM 1). Tissue: Fetal liver.
[8]	"Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46." Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W., Hauri H.-P. J. Biol. Chem. 279:47242-47253(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ERGIC1.
[9]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-241, MASS SPECTROMETRY. Tissue: Liver.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-297.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF308298 mRNA. Translation: AAG48265.1. Different initiation. AF151812 mRNA. Translation: AAD34049.1. AK074550 mRNA. Translation: BAC11054.1. AF077030 mRNA. Translation: AAD27763.1. AL121586 Genomic DNA. Translation: CAB89412.1. AL121586 Genomic DNA. Translation: CAI42842.1. BC009765 mRNA. Translation: AAH09765.1. BC014014 mRNA. Translation: AAH14014.1. AF116614 mRNA. Translation: AAF71038.1. Different initiation.
IPI	IPI00006438. IPI00446875. IPI00552115.
RefSeq	NP_057050.1. NM_015966.2. NP_938408.1. NM_198398.1.
UniGene	Hs.472558.
3D structure databases
ProteinModelPortal	Q9Y282.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y282. 2 interactions.
STRING	9606.ENSP00000349970.
PTM databases
PhosphoSite	Q9Y282.
Polymorphism databases
DMDM	37999823.
Proteomic databases
PaxDb	Q9Y282.
PRIDE	Q9Y282.
Protocols and materials databases
DNASU	51614.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000348547; ENSP00000341358; ENSG00000125991. ENST00000357394; ENSP00000349970; ENSG00000125991.
GeneID	51614.
KEGG	hsa:51614.
UCSC	uc002xcq.1. human. uc002xcs.3. human. uc002xct.3. human.
Organism-specific databases
CTD	51614.
GeneCards	GC20P034129.
HGNC	HGNC:15927. ERGIC3.
HPA	HPA015242. HPA015968.
neXtProt	NX_Q9Y282.
PharmGKB	PA38050.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG260258.
HOGENOM	HOG000210954.
HOVERGEN	HBG105706.
OrthoDB	EOG4CC41G.
Gene expression databases
ArrayExpress	Q9Y282.
Bgee	Q9Y282.
CleanEx	HS_ERGIC3.
Genevestigator	Q9Y282.
GermOnline	ENSG00000125991. Homo sapiens.
Family and domain databases
InterPro	IPR012936. Erv_C. [Graphical view]
Pfam	PF07970. COPIIcoated_ERV. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ERGIC3. human.
GenomeRNAi	51614.
NextBio	55518.

Entry information

Entry name

ERGI3_HUMAN

Accession

Primary (citable) accession number: Q9Y282
Secondary accession number(s): Q5JWS3 , Q6ZWP7, Q9H276, Q9P1L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.