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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 2

Gene

HS3ST2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in GlcA2S-GlcNS. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei205PAPSBy similarity1
Binding sitei213PAPSBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 128PAPSBy similarity5
Nucleotide bindingi330 – 334PAPSBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS04557-MONOMER.
ZFISH:HS04557-MONOMER.
BRENDAi2.8.2.29. 2681.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 2 (EC:2.8.2.29)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2
Short name:
3-OST-2
Short name:
Heparan sulfate 3-O-sulfotransferase 2
Short name:
h3-OST-2
Gene namesi
Name:HS3ST2
Synonyms:3OST2
ORF Names:UNQ2442/PRO5004
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:5195. HS3ST2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicSequence analysisAdd BLAST19
Transmembranei20 – 39Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini40 – 367LumenalSequence analysisAdd BLAST328

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9956.
OpenTargetsiENSG00000122254.
PharmGKBiPA29468.

Polymorphism and mutation databases

BioMutaiHS3ST2.
DMDMi61214416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852141 – 367Heparan sulfate glucosamine 3-O-sulfotransferase 2Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi102N-linked (GlcNAc...)Sequence analysis1
Glycosylationi193N-linked (GlcNAc...)Sequence analysis1
Glycosylationi235N-linked (GlcNAc...)Sequence analysis1
Glycosylationi306N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi313 ↔ 325By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Y278.
PRIDEiQ9Y278.

PTM databases

PhosphoSitePlusiQ9Y278.

Expressioni

Tissue specificityi

Highly expressed in the brain and weakly expressed in the heart, placenta, lung and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000122254.
CleanExiHS_HS3ST2.
ExpressionAtlasiQ9Y278. baseline and differential.
GenevisibleiQ9Y278. HS.

Organism-specific databases

HPAiHPA017736.

Interactioni

Protein-protein interaction databases

BioGridi115281. 11 interactors.
STRINGi9606.ENSP00000261374.

Structurei

3D structure databases

ProteinModelPortaliQ9Y278.
SMRiQ9Y278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni146 – 152Substrate bindingBy similarity7
Regioni177 – 180Substrate bindingBy similarity4
Regioni245 – 246Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9Y278.
KOiK07808.
OMAiCYSLLCC.
OrthoDBiEOG091G0CS5.
PhylomeDBiQ9Y278.
TreeFamiTF350755.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRVLGRAG PPQPRRARRL LFAFTLSLSC TYLCYSFLCC CDDLGRSRLL
60 70 80 90 100
GAPRCLRGPS AGGQKLLQKS RPCDPSGPTP SEPSAPSAPA AAVPAPRLSG
110 120 130 140 150
SNHSGSPKLG TKRLPQALIV GVKKGGTRAV LEFIRVHPDV RALGTEPHFF
160 170 180 190 200
DRNYGRGLDW YRSLMPRTLE SQITLEKTPS YFVTQEAPRR IFNMSRDTKL
210 220 230 240 250
IVVVRNPVTR AISDYTQTLS KKPDIPTFEG LSFRNRTLGL VDVSWNAIRI
260 270 280 290 300
GMYVLHLESW LQYFPLAQIH FVSGERLITD PAGEMGRVQD FLGIKRFITD
310 320 330 340 350
KHFYFNKTKG FPCLKKTESS LLPRCLGKSK GRTHVQIDPE VIDQLREFYR
360
PYNIKFYETV GQDFRWE
Length:367
Mass (Da):41,501
Last modified:November 1, 1999 - v1
Checksum:iF63EACDD4721607C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052530339P → A.Corresponds to variant rs17725080dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105374 mRNA. Translation: AAD30206.1.
AF105375 mRNA. Translation: AAD30207.1.
AY359095 mRNA. Translation: AAQ89453.1.
BC093734 mRNA. Translation: AAH93734.1.
BC093736 mRNA. Translation: AAH93736.1.
CCDSiCCDS10606.1.
RefSeqiNP_006034.1. NM_006043.1.
UniGeneiHs.115830.
Hs.733610.

Genome annotation databases

EnsembliENST00000261374; ENSP00000261374; ENSG00000122254.
GeneIDi9956.
KEGGihsa:9956.
UCSCiuc002dli.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105374 mRNA. Translation: AAD30206.1.
AF105375 mRNA. Translation: AAD30207.1.
AY359095 mRNA. Translation: AAQ89453.1.
BC093734 mRNA. Translation: AAH93734.1.
BC093736 mRNA. Translation: AAH93736.1.
CCDSiCCDS10606.1.
RefSeqiNP_006034.1. NM_006043.1.
UniGeneiHs.115830.
Hs.733610.

3D structure databases

ProteinModelPortaliQ9Y278.
SMRiQ9Y278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115281. 11 interactors.
STRINGi9606.ENSP00000261374.

PTM databases

PhosphoSitePlusiQ9Y278.

Polymorphism and mutation databases

BioMutaiHS3ST2.
DMDMi61214416.

Proteomic databases

PaxDbiQ9Y278.
PRIDEiQ9Y278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261374; ENSP00000261374; ENSG00000122254.
GeneIDi9956.
KEGGihsa:9956.
UCSCiuc002dli.4. human.

Organism-specific databases

CTDi9956.
DisGeNETi9956.
GeneCardsiHS3ST2.
HGNCiHGNC:5195. HS3ST2.
HPAiHPA017736.
MIMi604056. gene.
neXtProtiNX_Q9Y278.
OpenTargetsiENSG00000122254.
PharmGKBiPA29468.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9Y278.
KOiK07808.
OMAiCYSLLCC.
OrthoDBiEOG091G0CS5.
PhylomeDBiQ9Y278.
TreeFamiTF350755.

Enzyme and pathway databases

BioCyciMetaCyc:HS04557-MONOMER.
ZFISH:HS04557-MONOMER.
BRENDAi2.8.2.29. 2681.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Miscellaneous databases

GeneWikiiHS3ST2.
GenomeRNAii9956.
PROiQ9Y278.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000122254.
CleanExiHS_HS3ST2.
ExpressionAtlasiQ9Y278. baseline and differential.
GenevisibleiQ9Y278. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHS3S2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y278
Secondary accession number(s): Q52LZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.