Reviewed, UniProtKB/Swiss-Prot Q9Y275 (TN13B_HUMAN)

Names and origin

Protein names

Recommended name:
    Tumor necrosis factor ligand superfamily member 13B
Alternative name(s):
    TNF- and APOL-related leukocyte expressed ligand 1
      Short name=TALL-1
    B lymphocyte stimulator
      Short name=BLyS
    B cell-activating factor
    BAFF
    Dendritic cell-derived TNF-like molecule
    CD_antigen=CD257
Cleaved into the following 2 chains:
    1- Recommended name:
            Tumor necrosis factor ligand superfamily member 13b, membrane form
    2- Recommended name:
            Tumor necrosis factor ligand superfamily member 13b, soluble form

Gene names

Name:	TNFSF13B
Synonyms:	BAFF, BLYS, TALL1, TNFSF20, ZTNF4
ORF Names:	UNQ401/PRO738

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Subunit structure	Homotrimer.
Subcellular location	Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted.
Tissue specificity	Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Induction	Up-regulated by exposure to interferon-gamma. Down-regulated by phorbol myristate acetate/ionomycin treatment.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing. N-glycosylated.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Cytokine
PTM	Cleavage on pair of basic residues Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	positive regulation of cell proliferation Ref.2 Traceable author statement. Source: ProtInc signal transduction Traceable author statement. Source: ProtInc
Cellular component	soluble fraction Traceable author statement. Source: ProtInc
Molecular function	receptor binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TNFRSF13B	O14836	4	EBI-519169,EBI-519160
TNFRSF17	Q02223	1	EBI-519169,EBI-519945

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Tumor necrosis factor ligand superfamily member 13b, membrane form

PRO_0000034528

Chain

134 – 285

152

Tumor necrosis factor ligand superfamily member 13b, soluble form

PRO_0000034529

Regions

Topological domain

1 – 46

46

Cytoplasmic Potential

Transmembrane

47 – 67

21

Signal-anchor for type II membrane protein Potential

Topological domain

68 – 285

218

Extracellular Potential

Sites

Site

133 – 134

2

Cleavage

Amino acid modifications

Glycosylation

124

1

N-linked (GlcNAc...)

Glycosylation

242

1

N-linked (GlcNAc...) (high mannose)

Disulfide bond

232 ↔ 245

Natural variations

Natural variant

105

1

A → T

VAR_013483

Secondary structure

1

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285

Helix Strand Turn

Details...

Beta strand

146 – 151

6

Beta strand

158 – 160

3

Beta strand

163 – 165

3

Beta strand

168 – 181

14

Beta strand

184 – 187

4

Beta strand

191 – 201

11

Beta strand

205 – 215

11

Beta strand

225 – 235

11

Beta strand

238 – 240

3

Beta strand

243 – 253

11

Beta strand

258 – 265

8

Turn

274 – 276

3

Beta strand

277 – 283

7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y275-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 48ED0D7AB38C8867	FASTA	285	31,223
	10 20 30 40 50 60 MDDSTEREQS RLTSCLKKRE EMKLKECVSI LPRKESPSVR SSKDGKLLAA TLLLALLSCC 70 80 90 100 110 120 LTVVSFYQVA ALQGDLASLR AELQGHHAEK LPAGAGAPKA GLEEAPAVTA GLKIFEPPAP 130 140 150 160 170 180 GEGNSSQNSR NKRAVQGPEE TVTQDCLQLI ADSETPTIQK GSYTFVPWLL SFKRGSALEE 190 200 210 220 230 240 KENKILVKET GYFFIYGQVL YTDKTYAMGH LIQRKKVHVF GDELSLVTLF RCIQNMPETL 250 260 270 280 PNNSCYSAGI AKLEEGDELQ LAIPRENAQI SLDGDVTFFG ALKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TALL-1 is a novel member of the TNF family that is down-regulated by mitogens." Shu H.-B., Hu W.-H., Johnson H. J. Leukoc. Biol. 65:680-683(1999) [PubMed: 10331498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth." Schneider P., MacKay F., Steiner V., Hofmann K., Bodmer J.-L., Holler N., Ambrose C., Lawton P., Bixler S., Acha-Orbea H., Valmori D., Romero P., Werner-Favre C., Zubler R.H., Browning J.L., Tschopp J. J. Exp. Med. 189:1747-1756(1999) [PubMed: 10359578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 134-148.
[3]	"BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator." Moore P.A., Belvedere O., Orr A., Pieri K., LaFleur D.W., Feng P., Soppet D., Charters M., Gentz R., Parmelee D., Li Y., Galperina O., Giri J., Roschke V., Nardelli B., Carrell J., Sosnovtseva S., Greenfield W. , Ruben S.M., Olsen H.S., Fikes J., Hilbert D.M. Science 285:260-263(1999) [PubMed: 10398604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Monocyte and Neutrophil.
[4]	"Homo sapiens homolog of tumor necrosis factor." Farrah T., Gross J., Piddington C., O'Hara P. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[5]	"A novel dendritic cell-derived TNF-like molecule." Zhang W., Wan T., Yu Y., Cao X. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Dendritic cell.
[6]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[8]	"New polymorphisms of human BLyS gene." Kawasaki A., Tsuchiya N., Fukazawa T., Hashimoto H., Tokunaga K. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-135, VARIANT THR-105.
[9]	"APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity." Yu G., Boone T., Delaney J., Hawkins N., Kelley M.J., Ramakrishnan M., McCabe S., Qiu W.R., Kornuc M., Xia X.-Z., Guo J., Stolina M., Boyle W.J., Sarosi I., Hsu H., Senaldi G., Theill L.E. Nat. Immunol. 1:252-256(2000) [PubMed: 10973284] [Abstract] Cited for: FUNCTION.
[10]	"Crystal structure of sTALL-1 reveals a virus-like assembly of TNF family ligands." Liu Y., Xu L., Opalka N., Kappler J., Shu H.-B., Zhang G. Cell 108:383-394(2002) [PubMed: 11853672] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 142-285.
[11]	"Crystal structure of extracellular human BAFF, a TNF family member that stimulates B lymphocytes." Karpusas M., Cachero T.G., Qian F., Boriack-Sjodin A., Mullen C., Strauch K., Hsu Y.-M., Kalled S.L. J. Mol. Biol. 315:1145-1154(2002) [PubMed: 11827482] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 136-285.
[12]	"Structural basis of BLyS receptor recognition." Oren D.A., Li Y., Volovik Y., Morris T.S., Dharia C., Das K., Galperina O., Gentz R., Arnold E. Nat. Struct. Biol. 9:288-292(2002) [PubMed: 11862220] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 134-285.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Proteic grace - Issue 77 of December 2006

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF136293 mRNA. Translation: AAD29421.1.
AF116456 mRNA. Translation: AAD25356.1.
AF132600 mRNA. Translation: AAD21092.1.
AF186114 mRNA. Translation: AAF01432.1.
AF134715 mRNA. Translation: AAF60219.1.
AB073225 Genomic DNA. Translation: BAB90856.1.
AY358881 mRNA. Translation: AAQ89240.1.
BC020674 mRNA. Translation: AAH20674.1.

RefSeq

NP_006564.1.

UniGene

Hs.525157

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JH5	X-ray	3.00	A/B/C/D/E/F/G/H/I/J	142-285	[»]
1KD7	X-ray	2.80	A/B/C/K/L/M	136-285	[»]
1KXG	X-ray	2.00	A/B/C/D/E/F	134-285	[»]
1OQD	X-ray	2.60	A/B/C/D/E/F/G/H/I/J	142-285	[»]
1OQE	X-ray	2.50	A/B/C/D/E/F/G/H/I/J	142-285	[»]
1OSG	X-ray	3.00	A/B/C/D/E/F	82-285	[»]
1OTZ	X-ray	3.30	0/1/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x	138-285	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y275.

Genome annotation databases

Ensembl

ENSG00000102524. Homo sapiens. [Contig view]

GeneID

10673.

KEGG

hsa:10673.

Organism-specific databases

H-InvDB

HIX0011450.

HGNC

HGNC:11929. TNFSF13B.

HPA

CAB009188.

MIM

603969. gene.

PharmGKB

PA434.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q9Y275.

HOVERGEN

Q9Y275.

Gene expression databases

ArrayExpress

Q9Y275.

CleanEx

HS_TNFSF13B.

GermOnline

ENSG00000102524. Homo sapiens.

Family and domain databases

InterPro

IPR006052. TNF_family.
IPR008983. Tumour_necrosis_fac-like.
[Graphical view]

Gene3D

G3DSA:2.60.120.40. Tumour_necrosis_fac-like. 1 hit.

Pfam

PF00229. TNF. 1 hit.
[Graphical view]

SMART

SM00207. TNF. 1 hit.
[Graphical view]

PROSITE

PS00251. TNF_1. False negative.
PS50049. TNF_2. 1 hit.
[Graphical view]

BLOCKS

Search...

ProtoNet

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Other Resources

NextBio

40587.

SOURCE

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Entry information

Entry name

TN13B_HUMAN

Accession

Primary (citable) accession number: Q9Y275

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)