Reviewed, UniProtKB/Swiss-Prot Q9Y266 (NUDC_HUMAN)

Names and origin

Protein names

Recommended name:
    Nuclear migration protein nudC
Alternative name(s):
    Nuclear distribution protein C homolog

Gene names

Name:

NUDC

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in neurogenesis and neuronal migration By similarity. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
Subunit structure	Binds PLK1. Binds PAFAH1B1 By similarity. Part of a complex containing PLK1, NUDC, dynein and dynactin.
Subcellular location	Cytoplasm › cytoskeleton. Nucleus. Note: In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. Ref.1 Ref.10
Tissue specificity	Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart. Ref.1 Ref.2
Induction	Up-regulated in actively dividing hematopoietic precursor cells. Up-regulated in cultured erythroleukemia TF-1 cells by granulocyte-macrophage colony-stimulating factor. Strongly down-regulated during maturation of erythroid precursor cells. Ref.1
Post-translational modification	Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases. Ref.9 Ref.11 Ref.12 Ref.13
Sequence similarities	Belongs to the nudC family. Contains 1 CS domain.
Sequence caution	The sequence BAA76628.1 differs from that shown. Reason: Frameshift at positions 140, 231 and 273. The sequence CAI13560.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI13563.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW cell proliferation Ref.1 Traceable author statement. Source: ProtInc mitosis Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from direct assay. Source: LIFEdb microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DGKE	P52429	1	EBI-357298,EBI-1057499

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Nuclear migration protein nudC		PRO_0000057990
Regions
	Domain	167 – 258	92	CS
	Coiled coil	60 – 134	75	Potential
	Motif	68 – 74	7	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	139	1	Phosphoserine Ref.11 Ref.12 Ref.13
	Modified residue	145	1	Phosphothreonine Ref.11 Ref.12 Ref.13
	Modified residue	274	1	Phosphoserine; by PLK1 Ref.9
	Modified residue	285	1	Phosphoserine Ref.12
	Modified residue	326	1	Phosphoserine; by PLK1 Ref.9
Experimental info
	Mutagenesis	274	1	S → A: Abolishes phosphorylation by PLK1; when associated with A-326. Ref.9
	Mutagenesis	326	1	S → A: Abolishes phosphorylation by PLK1; when associated with A-274. Ref.9
	Sequence conflict	19	1	Q → H in BAA76628. Ref.4
	Sequence conflict	69	1	T → N in CAB66659. Ref.6
	Sequence conflict	74	1	R → I in BAA76628. Ref.4
	Sequence conflict	111	1	E → K in BAA76628. Ref.4
	Sequence conflict	114	1	R → K in BAA76628. Ref.4
	Sequence conflict	128	1	H → P in BAA76628. Ref.4
	Sequence conflict	149	1	E → V in CAB66659. Ref.6
	Sequence conflict	169	1	L → P in CAB66659. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9Y266-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 34F591170F7594AF	FASTA	331	38,243
	10 20 30 40 50 60 MGGEQEEERF DGMLLAMAQQ HEGGVQELVN TFFSFLRRKT DFFIGGEEGM AEKLITQTFS 70 80 90 100 110 120 HHNQLAQKTR REKRARQEAE RREKAERAAR LAKEAKSETS GPQIKELTDE EAERLQLEID 130 140 150 160 170 180 QKKDAENHEA QLKNGSLDSP GKQDTEEDEE EDEKDKGKLK PNLGNGADLP NYRWTQTLSE 190 200 210 220 230 240 LDLAVPFCVN FRLKGKDMVV DIQRRHLRVG LKGQPAIIDG ELYNEVKVEE SSWLIEDGKV 250 260 270 280 290 300 VTVHLEKINK MEWWSRLVSS DPEINTKKIN PENSKLSDLD SETRSMVEKM MYDQRQKSMG 310 320 330 LPTSDEQKKQ EILKKFMDQH PEMDFSKAKF N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homolog of the fungal nuclear migration gene nudC is involved in normal and malignant human hematopoiesis." Miller B.A., Zhang M.-Y., Gocke C.D., De Souza C., Osmani A.H., Lynch C., Davies J., Bell L., Osmani S.A. Exp. Hematol. 27:742-750(1999) [PubMed: 10210332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Heart.
[2]	"Molecular cloning and characterization of the human NUDC gene." Matsumoto N., Ledbetter D.H. Hum. Genet. 104:498-504(1999) [PubMed: 10453739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"Human MNUDC protein gene." Song H., Peng Y., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[4]	"Unique genes expressed in fibroblasts of periodontal ligament." Yamamoto T., Takashiba S., Myokai F., Washio N., Nishimura F., Arai H., Murayama Y. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Periodontal ligament.
[5]	"Cloning and characterization of a novel human cDNA homology to murine SIG-92 mRNA." Li N.G., Yu L., Tu Q., Fu Q., Wang X.K., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye, Kidney, Lung and Skin.
[9]	"A role for Plk1 phosphorylation of NudC in cytokinesis." Zhou T., Aumais J.P., Liu X., Yu-Lee L.-Y., Erikson R.L. Dev. Cell 5:127-138(2003) [PubMed: 12852857] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLK1, IDENTIFICATION IN A COMPLEX WITH DYNACTIN AND DYNEIN, MUTAGENESIS OF SER-274 AND SER-326, PHOSPHORYLATION AT SER-274 AND SER-326.
[10]	"Role for NudC, a dynein-associated nuclear movement protein, in mitosis and cytokinesis." Aumais J.P., Williams S.N., Luo W., Nishino M., Caldwell K.A., Caldwell G.A., Lin S.-H., Yu-Lee L.-Y. J. Cell Sci. 116:1991-2003(2003) [PubMed: 12679384] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND THR-145, MASS SPECTROMETRY. Tissue: Epithelium.
[12]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139; THR-145 AND SER-285, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND THR-145, MASS SPECTROMETRY.
[14]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF130736 mRNA. Translation: AAD30517.1. AF125465 mRNA. Translation: AAD39921.1. AF100760 mRNA. Translation: AAD43024.1. AB019408 mRNA. Translation: BAA76628.1. Frameshift. AF086922 mRNA. Translation: AAP97152.1. AL136725 mRNA. Translation: CAB66659.1. AL356390 Genomic DNA. Translation: CAI13560.1. Sequence problems. AL356390 Genomic DNA. Translation: CAI13561.1. AL356390 Genomic DNA. Translation: CAI13563.1. Sequence problems. BC002399 mRNA. Translation: AAH02399.1. BC003132 mRNA. Translation: AAH03132.1. BC006147 mRNA. Translation: AAH06147.1. BC007280 mRNA. Translation: AAH07280.1. BC015153 mRNA. Translation: AAH15153.1. BC021139 mRNA. Translation: AAH21139.1.
IPI	IPI00550746.
RefSeq	NP_006591.1.
UniGene	Hs.263812
3D structure databases
SMR	Q9Y266. Positions 165-287.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y266. 18 interactions.
PTM databases
PhosphoSite	Q9Y266.
Proteomic databases
PeptideAtlas	Q9Y266.
PRIDE	Q9Y266.
Genome annotation databases
Ensembl	ENSG00000090273. Homo sapiens. [Contig view]
GeneID	10726.
KEGG	hsa:10726.
UCSC	uc001bng.1. human.
Organism-specific databases
GeneCards	GC01P027120.
H-InvDB	HIX0000323.
HGNC	HGNC:8045. NUDC.
MIM	610325. gene.
PharmGKB	PA31827.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9Y266.
HOVERGEN	Q9Y266.
OMA	Q9Y266. QPAIIDG.
Enzyme and pathway databases
Pathway_Interaction_DB	lis1pathway. Lissencephaly gene (LIS1) in neuronal migration and development.
Reactome	REACT_152. Cell Cycle, Mitotic.
Gene expression databases
ArrayExpress	Q9Y266.
Bgee	Q9Y266.
CleanEx	HS_NUDC.
GermOnline	ENSG00000090273. Homo sapiens.
Family and domain databases
InterPro	IPR017447. CS. IPR007052. CS_domain. [Graphical view]
Pfam	PF04969. CS. 1 hit. [Graphical view]
PROSITE	PS51203. CS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40723.
SOURCE	Search...

Entry information

Entry name

NUDC_HUMAN

Accession

Primary (citable) accession number: Q9Y266
Secondary accession number(s): Q5QP31 , Q5QP35, Q9H0N2, Q9Y2B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 1, 1999
Last modified:	July 7, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)