Reviewed,
UniProtKB/Swiss-Prot Q9Y266 (NUDC_HUMAN)
Last modified
July 7, 2009.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nuclear migration protein nudC Alternative name(s): Nuclear distribution protein C homolog | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 331 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays a role in neurogenesis and neuronal migration By similarity. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation. |
| Subunit structure | Binds PLK1. Binds PAFAH1B1 By similarity. Part of a complex containing PLK1, NUDC, dynein and dynactin. |
| Subcellular location | Cytoplasm › cytoskeleton. Nucleus. Note: In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. Ref.1 Ref.10 |
| Tissue specificity | Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart. Ref.1 Ref.2 |
| Induction | Up-regulated in actively dividing hematopoietic precursor cells. Up-regulated in cultured erythroleukemia TF-1 cells by granulocyte-macrophage colony-stimulating factor. Strongly down-regulated during maturation of erythroid precursor cells. Ref.1 |
| Post-translational modification | Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases. Ref.9 Ref.11 Ref.12 Ref.13 |
| Sequence similarities | Belongs to the nudC family. Contains 1 CS domain. |
| Sequence caution | The sequence BAA76628.1 differs from that shown. Reason: Frameshift at positions 140, 231 and 273. The sequence CAI13560.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI13563.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell cycle Cell division Mitosis |
| Cellular component | Cytoplasm Cytoskeleton Microtubule Nucleus |
| Domain | Coiled coil |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell division Inferred from electronic annotation. Source: UniProtKB-KW cell proliferation Ref.1Traceable author statement. Source: ProtInc mitosisInferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | cytoplasm Inferred from direct assay. Source: LIFEdb microtubuleInferred from electronic annotation. Source: UniProtKB-KW nucleusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 331 | 331 | Nuclear migration protein nudC | PRO_0000057990 | |||||
Regions | |||||||||
| Domain | 167 – 258 | 92 | CS | ||||||
| Coiled coil | 60 – 134 | 75 | Potential | ||||||
| Motif | 68 – 74 | 7 | Nuclear localization signal Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 139 | 1 | Phosphoserine Ref.11 Ref.12 Ref.13 | ||||||
| Modified residue | 145 | 1 | Phosphothreonine Ref.11 Ref.12 Ref.13 | ||||||
| Modified residue | 274 | 1 | Phosphoserine; by PLK1 Ref.9 | ||||||
| Modified residue | 285 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 326 | 1 | Phosphoserine; by PLK1 Ref.9 | ||||||
Experimental info | |||||||||
| Mutagenesis | 274 | 1 | S → A: Abolishes phosphorylation by PLK1; when associated with A-326. Ref.9 | ||||||
| Mutagenesis | 326 | 1 | S → A: Abolishes phosphorylation by PLK1; when associated with A-274. Ref.9 | ||||||
| Sequence conflict | 19 | 1 | Q → H in BAA76628. Ref.4 | ||||||
| Sequence conflict | 69 | 1 | T → N in CAB66659. Ref.6 | ||||||
| Sequence conflict | 74 | 1 | R → I in BAA76628. Ref.4 | ||||||
| Sequence conflict | 111 | 1 | E → K in BAA76628. Ref.4 | ||||||
| Sequence conflict | 114 | 1 | R → K in BAA76628. Ref.4 | ||||||
| Sequence conflict | 128 | 1 | H → P in BAA76628. Ref.4 | ||||||
| Sequence conflict | 149 | 1 | E → V in CAB66659. Ref.6 | ||||||
| Sequence conflict | 169 | 1 | L → P in CAB66659. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A homolog of the fungal nuclear migration gene nudC is involved in normal and malignant human hematopoiesis." Miller B.A., Zhang M.-Y., Gocke C.D., De Souza C., Osmani A.H., Lynch C., Davies J., Bell L., Osmani S.A. Exp. Hematol. 27:742-750(1999) [PubMed: 10210332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Heart. |
| [2] | "Molecular cloning and characterization of the human NUDC gene." Matsumoto N., Ledbetter D.H. Hum. Genet. 104:498-504(1999) [PubMed: 10453739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. |
| [3] | "Human MNUDC protein gene." Song H., Peng Y., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary. |
| [4] | "Unique genes expressed in fibroblasts of periodontal ligament." Yamamoto T., Takashiba S., Myokai F., Washio N., Nishimura F., Arai H., Murayama Y. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Periodontal ligament. |
| [5] | "Cloning and characterization of a novel human cDNA homology to murine SIG-92 mRNA." Li N.G., Yu L., Tu Q., Fu Q., Wang X.K., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [6] | "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A.Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
| [7] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [8] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye, Kidney, Lung and Skin. |
| [9] | "A role for Plk1 phosphorylation of NudC in cytokinesis." Zhou T., Aumais J.P., Liu X., Yu-Lee L.-Y., Erikson R.L. Dev. Cell 5:127-138(2003) [PubMed: 12852857] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLK1, IDENTIFICATION IN A COMPLEX WITH DYNACTIN AND DYNEIN, MUTAGENESIS OF SER-274 AND SER-326, PHOSPHORYLATION AT SER-274 AND SER-326. |
| [10] | "Role for NudC, a dynein-associated nuclear movement protein, in mitosis and cytokinesis." Aumais J.P., Williams S.N., Luo W., Nishino M., Caldwell K.A., Caldwell G.A., Lin S.-H., Yu-Lee L.-Y. J. Cell Sci. 116:1991-2003(2003) [PubMed: 12679384] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [11] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND THR-145, MASS SPECTROMETRY. Tissue: Epithelium. |
| [12] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139; THR-145 AND SER-285, MASS SPECTROMETRY. |
| [13] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND THR-145, MASS SPECTROMETRY. |
| [14] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| AF130736 mRNA. Translation: AAD30517.1. AF125465 mRNA. Translation: AAD39921.1. AF100760 mRNA. Translation: AAD43024.1. AB019408 mRNA. Translation: BAA76628.1. Frameshift. AF086922 mRNA. Translation: AAP97152.1. AL136725 mRNA. Translation: CAB66659.1. AL356390 Genomic DNA. Translation: CAI13560.1. Sequence problems. AL356390 Genomic DNA. Translation: CAI13561.1. AL356390 Genomic DNA. Translation: CAI13563.1. Sequence problems. BC002399 mRNA. Translation: AAH02399.1. BC003132 mRNA. Translation: AAH03132.1. BC006147 mRNA. Translation: AAH06147.1. BC007280 mRNA. Translation: AAH07280.1. BC015153 mRNA. Translation: AAH15153.1. BC021139 mRNA. Translation: AAH21139.1. | |
| IPI | IPI00550746. |
| RefSeq | NP_006591.1. |
| UniGene | Hs.263812 |
3D structure databases | |
| SMR | Q9Y266. Positions 165-287. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9Y266. 18 interactions. |
PTM databases | |
| PhosphoSite | Q9Y266. |
Proteomic databases | |
| PeptideAtlas | Q9Y266. |
| PRIDE | Q9Y266. |
Genome annotation databases | |
| Ensembl | ENSG00000090273. Homo sapiens. [Contig view] |
| GeneID | 10726. |
| KEGG | hsa:10726. |
| UCSC | uc001bng.1. human. |
Organism-specific databases | |
| GeneCards | GC01P027120. |
| H-InvDB | HIX0000323. |
| HGNC | HGNC:8045. NUDC. |
| MIM | 610325. gene. |
| PharmGKB | PA31827. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9Y266. |
| HOVERGEN | Q9Y266. |
| OMA | Q9Y266. QPAIIDG. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | lis1pathway. Lissencephaly gene (LIS1) in neuronal migration and development. |
| Reactome | REACT_152. Cell Cycle, Mitotic. |
Gene expression databases | |
| ArrayExpress | Q9Y266. |
| Bgee | Q9Y266. |
| CleanEx | HS_NUDC. |
| GermOnline | ENSG00000090273. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR017447. CS. IPR007052. CS_domain. [Graphical view] |
| Pfam | PF04969. CS. 1 hit. [Graphical view] |
| PROSITE | PS51203. CS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 40723. |
| SOURCE | Search... |
Entry information
| Entry name | NUDC_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9Y266 Secondary accession number(s): Q5QP31 Q9Y2B6 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


