Q9Y263 (PLAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase A-2-activating protein
Short name=PLA2P
Short name=PLAP

Gene names

Name:	PLAA
Synonyms:	PLAP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the maintenance of ubiquitin levels By similarity.
Subunit structure	Interacts with ubiquitin. Interacts with VCP. Ref.12
Domain	The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus. The PFU domain mediates interaction with ubiquitin.
Sequence similarities	Belongs to the WD repeat PLAP family. Contains 6 ARM repeats. Contains 1 PFU domain. Contains 1 PUL domain. Contains 7 WD repeats.
Sequence caution	The sequence AAD03030.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD42075.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD42075.1 differs from that shown. Reason: Frameshift at position 698. The sequence BAA92105.1 differs from that shown. Reason: Erroneous termination at position 545. Translated as Gln. The sequence BAD97264.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAB42881.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAH72641.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat WD repeat
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid metabolic process Traceable author statement. Source: ProtInc signal transduction Traceable author statement. Source: ProtInc
Molecular function	phospholipase A2 activator activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 795

795

Phospholipase A-2-activating protein

PRO_0000051130

Regions

Repeat

17 – 56

40

WD 1

Repeat

63 – 107

45

WD 2

Repeat

110 – 148

39

WD 3

Repeat

149 – 188

40

WD 4

Repeat

190 – 227

38

WD 5

Repeat

229 – 268

40

WD 6

Repeat

270 – 307

38

WD 7

Domain

366 – 465

100

PFU

Domain

533 – 794

262

PUL

Repeat

546 – 588

43

ARM 1

Repeat

589 – 620

32

ARM 2

Repeat

621 – 669

49

ARM 3

Repeat

670 – 715

46

ARM 4

Repeat

716 – 755

40

ARM 5

Repeat

756 – 795

40

ARM 6

Amino acid modifications

Modified residue

50

1

Phosphoserine Ref.9

Modified residue

529

1

N6-acetyllysine Ref.10

Experimental info

Sequence conflict

14

1

L → F in AAD42075. Ref.5

Sequence conflict

57

1

E → D in AAD42075. Ref.5

Sequence conflict

86

1

A → F in AAD42075. Ref.5

Sequence conflict

97

1

F → L in AAD42075. Ref.5

Sequence conflict

172

1

D → G in BAA91803. Ref.1

Sequence conflict

363

1

E → K in BAD97264. Ref.6

Sequence conflict

422

1

T → A in AAD42075. Ref.5

Sequence conflict

520

1

N → S in BAA92105. Ref.1

Sequence conflict

531

1

M → L in AAD42075. Ref.5

Sequence conflict

541

1

V → L in AAD42075. Ref.5

Sequence conflict

746

1

L → P in AAD42075. Ref.5

Secondary structure

1

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795

Helix Strand Turn

Details...

Helix

548 – 551

4

Helix

555 – 558

4

Helix

564 – 566

3

Helix

570 – 584

15

Helix

593 – 603

11

Turn

607 – 609

3

Helix

611 – 620

10

Helix

624 – 631

8

Helix

638 – 645

8

Helix

653 – 665

13

Helix

674 – 678

5

Helix

680 – 688

9

Helix

689 – 691

3

Helix

696 – 715

20

Helix

724 – 733

10

Helix

739 – 748

10

Helix

757 – 765

9

Helix

771 – 777

7

Helix

782 – 792

11

Sequences

	Sequence		Length	Mass (Da)
	Q9Y263 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: D6E7330AC9891637	FASTA	795	87,157
	10 20 30 40 50 60 MTSGATRYRL SCSLRGHELD VRGLVCCAYP PGAFVSVSRD RTTRLWAPDS PNRSFTEMHC 70 80 90 100 110 120 MSGHSNFVSC VCIIPSSDIY PHGLIATGGN DHNICIFSLD SPMPLYILKG HKNTVCSLSS 130 140 150 160 170 180 GKFGTLLSGS WDTTAKVWLN DKCMMTLQGH TAAVWAVKIL PEQGLMLTGS ADKTVKLWKA 190 200 210 220 230 240 GRCERTFSGH EDCVRGLAIL SETEFLSCAN DASIRRWQIT GECLEVYYGH TNYIYSISVF 250 260 270 280 290 300 PNCRDFVTTA EDRSLRIWKH GECAQTIRLP AQSIWCCCVL DNGDIVVGAS DGIIRVFTES 310 320 330 340 350 360 EDRTASAEEI KAFEKELSHA TIDSKTGDLG DINAEQLPGR EHLNEPGTRE GQTRLIRDGE 370 380 390 400 410 420 KVEAYQWSVS EGRWIKIGDV VGSSGANQQT SGKVLYEGKE FDYVFSIDVN EGGPSYKLPY 430 440 450 460 470 480 NTSDDPWLTA YNFLQKNDLN PMFLDQVAKF IIDNTKGQML GLGNPSFSDP FTGGGRYVPG 490 500 510 520 530 540 SSGSSNTLPT ADPFTGAGRY VPGSASMGTT MAGVDPFTGN SAYRSAASKT MNIYFPKKEA 550 560 570 580 590 600 VTFDQANPTQ ILGKLKELNG TAPEEKKLTE DDLILLEKIL SLICNSSSEK PTVQQLQILW 610 620 630 640 650 660 KAINCPEDIV FPALDILRLS IKHPSVNENF CNEKEGAQFS SHLINLLNPK GKPANQLLAL 670 680 690 700 710 720 RTFCNCFVGQ AGQKLMMSQR ESLMSHAIEL KSGSNKNIHI ALATLALNYS VCFHKDHNIE 730 740 750 760 770 780 GKAQCLSLIS TILEVVQDLE ATFRLLVALG TLISDDSNAV QLAKSLGVDS QIKKYSSVSE 790 PAKVSECCRF ILNLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	"Chromosomal localization of phospholipase A2 activating protein, an ets2 target gene, to 9p21." Beatty B., Qi S., Pienkowska M., Scherer S.W., Testa J.R., Cheng J.Q., Herbrick J.-A., Scheidl T., Zhang Z., Kola I., Seth A. Genomics 62:529-532(1999) [PubMed: 10644453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-795.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 48-795. Tissue: Kidney.
[7]	"Cloning of the human phospholipase A2 activating protein (hPLAP) gene on the chromosome 9p21 melanoma deleted region." Ruiz A., Nadal M., Puig S., Estivill X. Gene 239:155-161(1999) [PubMed: 10571045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-795. Tissue: Fetal brain.
[8]	"Molecular characterization of cDNA for phospholipase A2-activating protein." Chopra A.K., Ribardo D.A., Wood T.G., Prusak D.J., Xu X.-J., Peterson J.W. Biochim. Biophys. Acta 1444:125-130(1999) [PubMed: 9931468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-795.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-529, MASS SPECTROMETRY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein." Fu Q.-S., Zhou C.-J., Gao H.-C., Jiang Y.-J., Zhou Z.-R., Hong J., Yao W.-M., Song A.-X., Lin D.-H., Hu H.-Y. J. Biol. Chem. 284:19043-19052(2009) [PubMed: 19423704] [Abstract] Cited for: STRUCTURE BY NMR OF 386-465, INTERACTION WITH UBIQUITIN.
[13]	"Structure and function of the PLAA/Ufd3-p97/Cdc48 complex." Qiu L., Pashkova N., Walker J.R., Winistorfer S., Allali-Hassani A., Akutsu M., Piper R., Dhe-Paganon S. J. Biol. Chem. 285:365-372(2010) [PubMed: 19887378] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 511-795 IN COMPLEX WITH VCP, ARM REPEATS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK001642 mRNA. Translation: BAA91803.1.
AK002143 mRNA. Translation: BAA92105.1. Sequence problems.
AL133608 mRNA. Translation: CAB63739.1.
AL356133 Genomic DNA. Translation: CAH72641.1. Sequence problems.
BC032551 mRNA. Translation: AAH32551.1.
AF145020 mRNA. Translation: AAD42075.1. Sequence problems.
AK223544 mRNA. Translation: BAD97264.1. Different initiation.
AJ238243 mRNA. Translation: CAB42881.1. Different initiation.
AF083395 mRNA. Translation: AAD03030.1. Different initiation.

IPI

IPI00218465.

PIR

T43447.

RefSeq

NP_001026859.1. NM_001031689.2.

UniGene

Hs.27182.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K89	NMR	-	A	386-465	[»]
2K8A	NMR	-	A	386-465	[»]
2K8B	NMR	-	B	386-465	[»]
2K8C	NMR	-	B	386-465	[»]
3EBB	X-ray	1.90	A/B/C/D	511-795	[»]

ProteinModelPortal

Q9Y263.

SMR

Q9Y263. Positions 5-306, 386-795.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y263. 10 interactions.

STRING

Q9Y263.

PTM databases

PhosphoSite

Q9Y263.

Polymorphism databases

DMDM

108935868.

Proteomic databases

PRIDE

Q9Y263.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000397292; ENSP00000380460; ENSG00000137055.

GeneID

9373.

KEGG

hsa:9373.

UCSC

uc003zqd.1. human.

Organism-specific databases

CTD

9373.

GeneCards

GC09M026903.

H-InvDB

HIX0007960.

HGNC

HGNC:9043. PLAA.

HPA

CAB005035.
HPA020994.
HPA020996.

MIM

603873. gene.

neXtProt

NX_Q9Y263.

PharmGKB

PA33370.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08100.

GeneTree

ENSGT00550000074944.

HOVERGEN

HBG008204.

InParanoid

Q9Y263.

OMA

RSLRIWK.

OrthoDB

EOG4DJJVW.

PhylomeDB

Q9Y263.

Gene expression databases

ArrayExpress

Q9Y263.

Bgee

Q9Y263.

CleanEx

HS_PLAA.

Genevestigator

Q9Y263.

GermOnline

ENSG00000137055. Homo sapiens.

Family and domain databases

InterPro

IPR015155. PLAA_fam_Ub-bd_PFU.
IPR013535. PUL.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

KO

K14018.

Pfam

PF09070. PFU. 1 hit.
PF08324. PUL. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS50176. ARM_REPEAT. False negative.
PS51394. PFU. 1 hit.
PS51396. PUL. 1 hit.
PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

35110.

SOURCE

Search...

Entry information

Entry name

PLAP_HUMAN

Accession

Primary (citable) accession number: Q9Y263
Secondary accession number(s): Q53EU5 , Q5VY33, Q9NUL8, Q9NVE9, Q9UF53, Q9Y5L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.