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Q9Y257 (KCNK6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel subfamily K member 6
Alternative name(s):
Inward rectifying potassium channel protein TWIK-2
TWIK-originated similarity sequence
Gene names
Name:KCNK6
Synonyms:TOSS, TWIK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exhibits outward rectification in a physiological K+ gradient and mild inward rectification in symmetrical K+ conditions.

Subunit structure

Homodimer Potential.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Widespread expression, detected in all tissues tested except for skeletal muscle. Strongest expression in placenta, pancreas, heart, colon and spleen, lower levels detected in peripheral blood leukocytes, lung, liver, kidney and thymus. Lowest expression detected in brain.

Miscellaneous

Inhibited by internal acidification and, to a small degree, by zinc. Not inhibited by quinine, quinidine or barium.

Sequence similarities

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y257-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y257-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Potassium channel subfamily K member 6
PRO_0000101750

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2521Helical; Potential
Intramembrane90 – 11526Pore-forming; Name=Pore-forming 1; Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 17231Cytoplasmic Potential
Transmembrane173 – 19321Helical; Potential
Intramembrane199 – 22325Pore-forming; Name=Pore-forming 2; Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 31357Cytoplasmic Potential

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation851N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 134134Missing in isoform 2.
VSP_006692
Natural variant1501T → I.
Corresponds to variant rs35762773 [ dbSNP | Ensembl ].
VAR_052426
Natural variant2401V → I.
Corresponds to variant rs35496032 [ dbSNP | Ensembl ].
VAR_059842
Natural variant2591V → M.
Corresponds to variant rs34989303 [ dbSNP | Ensembl ].
VAR_052427

Experimental info

Mutagenesis531C → A: No channel activity. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 1379382DFB0575DE

FASTA31333,747
        10         20         30         40         50         60 
MRRGALLAGA LAAYAAYLVL GALLVARLEG PHEARLRAEL ETLRAQLLQR SPCVAAPALD 

        70         80         90        100        110        120 
AFVERVLAAG RLGRVVLANA SGSANASDPA WDFASALFFA STLITTVGYG YTTPLTDAGK 

       130        140        150        160        170        180 
AFSIAFALLG VPTTMLLLTA SAQRLSLLLT HVPLSWLSMR WGWDPRRAAC WHLVALLGVV 

       190        200        210        220        230        240 
VTVCFLVPAV IFAHLEEAWS FLDAFYFCFI SLSTIGLGDY VPGEAPGQPY RALYKVLVTV 

       250        260        270        280        290        300 
YLFLGLVAMV LVLQTFRHVS DLHGLTELIL LPPPCPASFN ADEDDRVDIL GPQPESHQQL 

       310 
SASSHTDYAS IPR 

« Hide

Isoform 2 [UniParc].

Checksum: 21805E679C6A83F6
Show »

FASTA17919,903

References

« Hide 'large scale' references
[1]"Identification and cloning of TWIK-originated similarity sequence (TOSS): a novel human 2-pore K+ channel principal subunit."
Pountney D.J., Gulkarov I., Vega-Saenz de Miera E., Holmes D., Saganich M., Rudy B., Artman M., Coetzee W.A.
FEBS Lett. 450:191-196(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"TWIK-2, a new weak inward rectifying member of the tandem pore domain potassium channel family."
Chavez R.A., Gray A.T., Zhao B.B., Kindler C.H., Mazurek M.J., Mehta Y., Forsayeth J.R., Yost C.S.
J. Biol. Chem. 274:7887-7892(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS OF CYS-53.
Tissue: Brain.
[3]Erratum
Chavez R.A., Gray A.T., Zhao B.B., Kindler C.H., Mazurek M.J., Mehta Y., Forsayeth J.R., Yost C.S.
J. Biol. Chem. 274:24440-24440(1999)
[4]"TWIK-2, an inactivating 2P domain K+ channel."
Patel A.J., Maingret F., Magnone V., Fosset M., Lazdunski M., Honore E.
J. Biol. Chem. 275:28722-28730(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION.
[5]"Genomic structure and mutation screening of the TWIK-2 gene."
Chen A.F., Gray A.T., Chen A.H., Kindler C.H., Mhatre A.N., Yost C.S., Lalwani A.K., Smith R.J.H.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF134149 mRNA. Translation: AAD22980.1.
AF117708 mRNA. Translation: AAD24000.1.
AF281302 mRNA. Translation: AAG10506.1.
AF281303 mRNA. Translation: AAG10507.1.
AJ297404, AJ297405 Genomic DNA. Translation: CAC15489.1.
BC004367 mRNA. Translation: AAH04367.1.
CCDSCCDS12513.1. [Q9Y257-1]
RefSeqNP_004814.1. NM_004823.1. [Q9Y257-1]
XP_005259488.1. XM_005259431.2. [Q9Y257-2]
XP_006723572.1. XM_006723509.1. [Q9Y257-1]
XP_006723573.1. XM_006723510.1. [Q9Y257-2]
UniGeneHs.240395.

3D structure databases

ProteinModelPortalQ9Y257.
SMRQ9Y257. Positions 20-262.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000263372.

Chemistry

DrugBankDB00308. Ibutilide.
DB00908. Quinidine.

Protein family/group databases

TCDB1.A.1.8.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteQ9Y257.

Polymorphism databases

DMDM13124108.

Proteomic databases

PaxDbQ9Y257.
PRIDEQ9Y257.

Protocols and materials databases

DNASU9424.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263372; ENSP00000263372; ENSG00000099337. [Q9Y257-1]
GeneID9424.
KEGGhsa:9424.
UCSCuc002oic.3. human. [Q9Y257-1]

Organism-specific databases

CTD9424.
GeneCardsGC19P038810.
H-InvDBHIX0202814.
HGNCHGNC:6281. KCNK6.
HPACAB022637.
HPA040184.
MIM603939. gene.
neXtProtNX_Q9Y257.
PharmGKBPA30063.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG290832.
HOGENOMHOG000286014.
HOVERGENHBG052237.
InParanoidQ9Y257.
KOK04917.
OMAPAVIFAH.
OrthoDBEOG7TTQ85.
PhylomeDBQ9Y257.
TreeFamTF313947.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

ArrayExpressQ9Y257.
BgeeQ9Y257.
CleanExHS_KCNK6.
GenevestigatorQ9Y257.

Family and domain databases

InterProIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR005408. 2pore_dom_K_chnl_TWIK.
IPR005409. 2pore_dom_K_chnl_TWIK2.
[Graphical view]
PfamPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSPR01333. 2POREKCHANEL.
PR01587. TWIK2CHANNEL.
PR01586. TWIKCHANNEL.
ProtoNetSearch...

Other

GeneWikiKCNK6.
GenomeRNAi9424.
NextBio35302.
PROQ9Y257.
SOURCESearch...

Entry information

Entry nameKCNK6_HUMAN
AccessionPrimary (citable) accession number: Q9Y257
Secondary accession number(s): Q9HB47
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM