Q9Y256 (FACE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CAAX prenyl protease 2
EC=3.4.22.-

Alternative name(s):
Farnesylated proteins-converting enzyme 2
Short name=FACE-2
Prenyl protein-specific endoprotease 2
RCE1 homolog
Short name=hRCE1

Gene names

Name:	RCE1
Synonyms:	FACE2, RCE1A, RCE1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Proteolytically removes the C-terminal three residues of farnesylated and geranylated proteins. Seems to be able to process K-Ras, N-Ras, H-Ras, RAP1B and G-gamma-1.
Enzyme regulation	Deubiquitination by USP17L2/USP17 negatively regulates the proteolytic activity toward Ras GTPases. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.
Tissue specificity	Ubiquitous.
Post-translational modification	Ubiquitinated. Undergoes 'Lys-48'-and 'Lys-63'-linked ubiquitination. 'Lys-48' ubiquitination induces its degradation. Deubiquitinated by USP17L2/USP17 that cleaves 'Lys-63'-linked ubiquitin chains. Ref.5
Sequence similarities	Belongs to the peptidase U48 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CAAX-box protein processing Inferred from mutant phenotype Ref.5. Source: UniProtKB proteolysis Traceable author statement Ref.2. Source: ProtInc
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.5. Source: UniProtKB integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	cysteine-type endopeptidase activity Inferred from direct assay Ref.5. Source: UniProtKB metalloendopeptidase activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	CAAX prenyl protease 2		PRO_0000194830
Regions
	Transmembrane	25 – 45	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	112 – 132	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Transmembrane	254 – 274	21	Helical; Potential
	Transmembrane	283 – 303	21	Helical; Potential
Natural variations
	Natural variant	326	1	P → A in a breast cancer sample; somatic mutation. Ref.6		VAR_036407

Sequences

	Sequence		Length	Mass (Da)
	Q9Y256 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: BA8F764651172BFA	FASTA	329	35,833
	10 20 30 40 50 60 MAALGGDGLR LLSVSRPERP PESAALGGLG PGLCCWVSVF SCLSLACSYV GSLYVWKSEL 70 80 90 100 110 120 PRDHPAVIKR RFTSVLVVSS LSPLCVLLWR ELTGIQPGTS LLTLMGFRLE GIFPAALLPL 130 140 150 160 170 180 LLTMILFLGP LMQLSMDCPC DLADGLKVVL APRSWARCLT DMRWLRNQVI APLTEELVFR 190 200 210 220 230 240 ACMLPMLAPC MGLGPAVFTC PLFFGVAHFH HIIEQLRFRQ SSVGNIFLSA AFQFSYTAVF 250 260 270 280 290 300 GAYTAFLFIR TGHLIGPVLC HSFCNYMGFP AVCAALEHPQ RRPLLAGYAL GVGLFLLLLQ 310 320 PLTDPKLYGS LPLCVLLERA GDSEAPLCS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and chromosomal location of two human genes encoding enzymes potentially involved in proteolytic maturation of farnesylated proteins." Freije J.M.P., Blay P., Pendas A.M., Cadinanos J., Crespo P., Lopez-Otin C. Genomics 58:270-280(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"Cloning and characterization of a mammalian prenyl protein-specific protease." Otto J.C., Kim E., Young S.G., Casey P.J. J. Biol. Chem. 274:8379-8382(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Human ras-converting enzyme (hRCE1) endoproteolytic activity on K-ras-derived peptides." Hollander I., Frommer E., Mallon R. Anal. Biochem. 286:129-137(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"USP17 regulates Ras activation and cell proliferation by blocking RCE1 activity." Burrows J.F., Kelvin A.A., McFarlane C., Burden R.E., McGrattan M.J., De la Vega M., Govender U., Quinn D.J., Dib K., Gadina M., Scott C.J., Johnston J.A. J. Biol. Chem. 284:9587-9595(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION, DEUBIQUITINATION BY USP17L2, SUBCELLULAR LOCATION, ENZYME REGULATION.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-326.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13835 mRNA. Translation: CAB46278.1. AF121951 mRNA. Translation: AAD22632.1. BC093726 mRNA. Translation: AAH93726.1. BC093728 mRNA. Translation: AAH93728.1.
IPI	IPI00031755.
RefSeq	NP_001027450.1. NM_001032279.1. NP_005124.1. NM_005133.2.
UniGene	Hs.654972.
3D structure databases
ProteinModelPortal	Q9Y256.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000309163.
Protein family/group databases
MEROPS	M79.002.
PTM databases
PhosphoSite	Q9Y256.
Polymorphism databases
DMDM	13431529.
Proteomic databases
PaxDb	Q9Y256.
PRIDE	Q9Y256.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309657; ENSP00000309163; ENSG00000173653.
GeneID	9986.
KEGG	hsa:9986.
UCSC	uc001ojk.1. human.
Organism-specific databases
CTD	9986.
GeneCards	GC11P066611.
HGNC	HGNC:13721. RCE1.
MIM	605385. gene.
neXtProt	NX_Q9Y256.
PharmGKB	PA34302.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG264307.
HOGENOM	HOG000006037.
HOVERGEN	HBG002541.
InParanoid	Q9Y256.
KO	K08658.
OMA	CNHMGLP.
OrthoDB	EOG4KWJTR.
PhylomeDB	Q9Y256.
Enzyme and pathway databases
BRENDA	3.4.22.22. 2681.
Gene expression databases
ArrayExpress	Q9Y256.
Bgee	Q9Y256.
CleanEx	HS_RCE1.
Genevestigator	Q9Y256.
GermOnline	ENSG00000173653. Homo sapiens.
Family and domain databases
InterPro	IPR003675. CAAX_protease. [Graphical view]
Pfam	PF02517. Abi. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9Y256.
ChEMBL	CHEMBL3411.
GenomeRNAi	9986.
NextBio	37706.
SOURCE	Search...

Entry information

Entry name

FACE2_HUMAN

Accession

Primary (citable) accession number: Q9Y256
Secondary accession number(s): Q52LZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.