Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CAAX prenyl protease 2

Gene

RCE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proteolytically removes the C-terminal three residues of farnesylated and geranylated proteins. Seems to be able to process K-Ras, N-Ras, H-Ras, RAP1B and G-gamma-1.

Enzyme regulationi

Deubiquitination by USP17L2/USP17 negatively regulates the proteolytic activity toward Ras GTPases.1 Publication

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: UniProtKB
  • metalloendopeptidase activity Source: ProtInc

GO - Biological processi

  • CAAX-box protein processing Source: UniProtKB
  • proteolysis Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.4.99.B1. 2681.

Protein family/group databases

MEROPSiM79.002.

Names & Taxonomyi

Protein namesi
Recommended name:
CAAX prenyl protease 2 (EC:3.4.22.-)
Alternative name(s):
Farnesylated proteins-converting enzyme 2
Short name:
FACE-2
Prenyl protein-specific endoprotease 2
RCE1 homolog
Short name:
hRCE1
Gene namesi
Name:RCE1
Synonyms:FACE2, RCE1A, RCE1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:13721. RCE1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521HelicalSequence AnalysisAdd
BLAST
Transmembranei75 – 9521HelicalSequence AnalysisAdd
BLAST
Transmembranei112 – 13221HelicalSequence AnalysisAdd
BLAST
Transmembranei186 – 20621HelicalSequence AnalysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence AnalysisAdd
BLAST
Transmembranei254 – 27421HelicalSequence AnalysisAdd
BLAST
Transmembranei283 – 30321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34302.

Polymorphism and mutation databases

BioMutaiRCE1.
DMDMi13431529.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 329328CAAX prenyl protease 2PRO_0000194830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Post-translational modificationi

Ubiquitinated. Undergoes 'Lys-48'-and 'Lys-63'-linked ubiquitination. 'Lys-48' ubiquitination induces its degradation. Deubiquitinated by USP17L2/USP17 that cleaves 'Lys-63'-linked ubiquitin chains.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

MaxQBiQ9Y256.
PaxDbiQ9Y256.
PRIDEiQ9Y256.

PTM databases

PhosphoSiteiQ9Y256.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiQ9Y256.
CleanExiHS_RCE1.
ExpressionAtlasiQ9Y256. baseline.
GenevisibleiQ9Y256. HS.

Interactioni

Protein-protein interaction databases

BioGridi115307. 1 interaction.
STRINGi9606.ENSP00000309163.

Structurei

3D structure databases

ProteinModelPortaliQ9Y256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase U48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG264307.
GeneTreeiENSGT00390000004124.
HOGENOMiHOG000006037.
HOVERGENiHBG002541.
InParanoidiQ9Y256.
KOiK08658.
OMAiYSAFLFA.
OrthoDBiEOG7F24TC.
PhylomeDBiQ9Y256.
TreeFamiTF313800.

Family and domain databases

InterProiIPR003675. CAAX_protease.
[Graphical view]
PfamiPF02517. Abi. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALGGDGLR LLSVSRPERP PESAALGGLG PGLCCWVSVF SCLSLACSYV
60 70 80 90 100
GSLYVWKSEL PRDHPAVIKR RFTSVLVVSS LSPLCVLLWR ELTGIQPGTS
110 120 130 140 150
LLTLMGFRLE GIFPAALLPL LLTMILFLGP LMQLSMDCPC DLADGLKVVL
160 170 180 190 200
APRSWARCLT DMRWLRNQVI APLTEELVFR ACMLPMLAPC MGLGPAVFTC
210 220 230 240 250
PLFFGVAHFH HIIEQLRFRQ SSVGNIFLSA AFQFSYTAVF GAYTAFLFIR
260 270 280 290 300
TGHLIGPVLC HSFCNYMGFP AVCAALEHPQ RRPLLAGYAL GVGLFLLLLQ
310 320
PLTDPKLYGS LPLCVLLERA GDSEAPLCS
Length:329
Mass (Da):35,833
Last modified:November 1, 1999 - v1
Checksum:iBA8F764651172BFA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti326 – 3261P → A in a breast cancer sample; somatic mutation. 1 Publication
VAR_036407

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13835 mRNA. Translation: CAB46278.1.
AF121951 mRNA. Translation: AAD22632.1.
BC093726 mRNA. Translation: AAH93726.1.
BC093728 mRNA. Translation: AAH93728.1.
CCDSiCCDS8151.1.
RefSeqiNP_001027450.1. NM_001032279.1.
NP_005124.1. NM_005133.2.
UniGeneiHs.654972.

Genome annotation databases

EnsembliENST00000309657; ENSP00000309163; ENSG00000173653.
GeneIDi9986.
KEGGihsa:9986.
UCSCiuc001ojk.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13835 mRNA. Translation: CAB46278.1.
AF121951 mRNA. Translation: AAD22632.1.
BC093726 mRNA. Translation: AAH93726.1.
BC093728 mRNA. Translation: AAH93728.1.
CCDSiCCDS8151.1.
RefSeqiNP_001027450.1. NM_001032279.1.
NP_005124.1. NM_005133.2.
UniGeneiHs.654972.

3D structure databases

ProteinModelPortaliQ9Y256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115307. 1 interaction.
STRINGi9606.ENSP00000309163.

Chemistry

BindingDBiQ9Y256.
ChEMBLiCHEMBL3411.

Protein family/group databases

MEROPSiM79.002.

PTM databases

PhosphoSiteiQ9Y256.

Polymorphism and mutation databases

BioMutaiRCE1.
DMDMi13431529.

Proteomic databases

MaxQBiQ9Y256.
PaxDbiQ9Y256.
PRIDEiQ9Y256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309657; ENSP00000309163; ENSG00000173653.
GeneIDi9986.
KEGGihsa:9986.
UCSCiuc001ojk.1. human.

Organism-specific databases

CTDi9986.
GeneCardsiGC11P066611.
HGNCiHGNC:13721. RCE1.
MIMi605385. gene.
neXtProtiNX_Q9Y256.
PharmGKBiPA34302.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG264307.
GeneTreeiENSGT00390000004124.
HOGENOMiHOG000006037.
HOVERGENiHBG002541.
InParanoidiQ9Y256.
KOiK08658.
OMAiYSAFLFA.
OrthoDBiEOG7F24TC.
PhylomeDBiQ9Y256.
TreeFamiTF313800.

Enzyme and pathway databases

BRENDAi3.4.99.B1. 2681.

Miscellaneous databases

GeneWikiiRCE1.
GenomeRNAii9986.
NextBioi37706.
PROiQ9Y256.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y256.
CleanExiHS_RCE1.
ExpressionAtlasiQ9Y256. baseline.
GenevisibleiQ9Y256. HS.

Family and domain databases

InterProiIPR003675. CAAX_protease.
[Graphical view]
PfamiPF02517. Abi. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and chromosomal location of two human genes encoding enzymes potentially involved in proteolytic maturation of farnesylated proteins."
    Freije J.M.P., Blay P., Pendas A.M., Cadinanos J., Crespo P., Lopez-Otin C.
    Genomics 58:270-280(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. "Cloning and characterization of a mammalian prenyl protein-specific protease."
    Otto J.C., Kim E., Young S.G., Casey P.J.
    J. Biol. Chem. 274:8379-8382(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Human ras-converting enzyme (hRCE1) endoproteolytic activity on K-ras-derived peptides."
    Hollander I., Frommer E., Mallon R.
    Anal. Biochem. 286:129-137(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: UBIQUITINATION, DEUBIQUITINATION BY USP17L2, SUBCELLULAR LOCATION, ENZYME REGULATION.
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-326.

Entry informationi

Entry nameiFACE2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y256
Secondary accession number(s): Q52LZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: June 24, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.