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Q9Y248

- PSF2_HUMAN

UniProt

Q9Y248 - PSF2_HUMAN

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Protein

DNA replication complex GINS protein PSF2

Gene
GINS2, PSF2, CGI-122, DC5, HSPC037
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. DNA strand elongation involved in DNA replication Source: Reactome
  2. mitotic cell cycle Source: Reactome
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_6776. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein PSF2
Alternative name(s):
GINS complex subunit 2
Gene namesi
Name:GINS2
Synonyms:PSF2
ORF Names:CGI-122, DC5, HSPC037
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:24575. GINS2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA145008313.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185DNA replication complex GINS protein PSF2PRO_0000194813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei180 – 1801Phosphothreonine1 Publication
Modified residuei182 – 1821Phosphoserine4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9Y248.
PaxDbiQ9Y248.
PRIDEiQ9Y248.

PTM databases

PhosphoSiteiQ9Y248.

Expressioni

Gene expression databases

ArrayExpressiQ9Y248.
BgeeiQ9Y248.
CleanExiHS_GINS2.
GenevestigatoriQ9Y248.

Organism-specific databases

HPAiHPA057285.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS3. GINS complex interacts with DNA primase in vitro.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CHEK2O960172EBI-747491,EBI-1180783
MCMBPQ9BTE32EBI-747491,EBI-749378

Protein-protein interaction databases

BioGridi119664. 18 interactions.
DIPiDIP-29332N.
IntActiQ9Y248. 3 interactions.
MINTiMINT-1469655.
STRINGi9606.ENSP00000253462.

Structurei

Secondary structure

1
185
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 108
Beta strandi13 – 219
Beta strandi26 – 283
Beta strandi31 – 333
Beta strandi42 – 454
Helixi46 – 549
Beta strandi57 – 604
Helixi68 – 8013
Beta strandi81 – 833
Helixi92 – 10312
Helixi104 – 1063
Beta strandi107 – 1093
Helixi110 – 13728
Beta strandi141 – 1444
Helixi150 – 17021
Turni172 – 1743

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30B/F1-185[»]
2EHOX-ray3.00C/G/K1-185[»]
2Q9QX-ray2.36A/E1-185[»]
ProteinModelPortaliQ9Y248.
SMRiQ9Y248. Positions 1-175.

Miscellaneous databases

EvolutionaryTraceiQ9Y248.

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS2/PSF2 family.

Phylogenomic databases

eggNOGiCOG5093.
HOGENOMiHOG000246603.
HOVERGENiHBG044983.
InParanoidiQ9Y248.
KOiK10733.
OMAiVPPEWME.
OrthoDBiEOG7GQXXJ.
PhylomeDBiQ9Y248.
TreeFamiTF314359.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR007257. GINS_Psf2.
IPR016906. GINS_Psf2_subgr.
[Graphical view]
PANTHERiPTHR12772. PTHR12772. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]
PIRSFiPIRSF028998. GINS_Psf2_subgr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y248-1 [UniParc]FASTAAdd to Basket

« Hide

MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI    50
NLKQRQKCRL LPPEWMDVEK LEKMRDHERK EETFTPMPSP YYMELTKLLL 100
NHASDNIPKA DEIRTLVKDM WDTRIAKLRV SADSFVRQQE AHAKLDNLTL 150
MEINTSGTFL TQALNHMYKL RTNLQPLEST QSQDF 185
Length:185
Mass (Da):21,428
Last modified:November 1, 1999 - v1
Checksum:i4F6A18B1ED76F93C
GO

Mass spectrometryi

Molecular mass is 98373±13 Da from positions 1 - 185. Determined by ESI. This is the measured mass for the GINS complex.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF125098 mRNA. Translation: AAD39915.1.
AF151880 mRNA. Translation: AAD34117.1.
AF201939 mRNA. Translation: AAF86875.1.
AK001275 mRNA. Translation: BAA91595.1.
CR457186 mRNA. Translation: CAG33467.1.
CH471114 Genomic DNA. Translation: EAW95443.1.
CH471114 Genomic DNA. Translation: EAW95444.1.
BC003186 mRNA. Translation: AAH03186.1.
BC010164 mRNA. Translation: AAH10164.1.
BC062444 mRNA. Translation: AAH62444.1.
CCDSiCCDS10953.1.
RefSeqiNP_057179.1. NM_016095.2.
UniGeneiHs.433180.

Genome annotation databases

EnsembliENST00000253462; ENSP00000253462; ENSG00000131153.
GeneIDi51659.
KEGGihsa:51659.
UCSCiuc002fja.3. human.

Polymorphism databases

DMDMi37999822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF125098 mRNA. Translation: AAD39915.1 .
AF151880 mRNA. Translation: AAD34117.1 .
AF201939 mRNA. Translation: AAF86875.1 .
AK001275 mRNA. Translation: BAA91595.1 .
CR457186 mRNA. Translation: CAG33467.1 .
CH471114 Genomic DNA. Translation: EAW95443.1 .
CH471114 Genomic DNA. Translation: EAW95444.1 .
BC003186 mRNA. Translation: AAH03186.1 .
BC010164 mRNA. Translation: AAH10164.1 .
BC062444 mRNA. Translation: AAH62444.1 .
CCDSi CCDS10953.1.
RefSeqi NP_057179.1. NM_016095.2.
UniGenei Hs.433180.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E9X X-ray 2.30 B/F 1-185 [» ]
2EHO X-ray 3.00 C/G/K 1-185 [» ]
2Q9Q X-ray 2.36 A/E 1-185 [» ]
ProteinModelPortali Q9Y248.
SMRi Q9Y248. Positions 1-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119664. 18 interactions.
DIPi DIP-29332N.
IntActi Q9Y248. 3 interactions.
MINTi MINT-1469655.
STRINGi 9606.ENSP00000253462.

PTM databases

PhosphoSitei Q9Y248.

Polymorphism databases

DMDMi 37999822.

Proteomic databases

MaxQBi Q9Y248.
PaxDbi Q9Y248.
PRIDEi Q9Y248.

Protocols and materials databases

DNASUi 51659.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000253462 ; ENSP00000253462 ; ENSG00000131153 .
GeneIDi 51659.
KEGGi hsa:51659.
UCSCi uc002fja.3. human.

Organism-specific databases

CTDi 51659.
GeneCardsi GC16M085709.
HGNCi HGNC:24575. GINS2.
HPAi HPA057285.
MIMi 610609. gene.
neXtProti NX_Q9Y248.
PharmGKBi PA145008313.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5093.
HOGENOMi HOG000246603.
HOVERGENi HBG044983.
InParanoidi Q9Y248.
KOi K10733.
OMAi VPPEWME.
OrthoDBi EOG7GQXXJ.
PhylomeDBi Q9Y248.
TreeFami TF314359.

Enzyme and pathway databases

Reactomei REACT_6776. Unwinding of DNA.

Miscellaneous databases

ChiTaRSi GINS2. human.
EvolutionaryTracei Q9Y248.
GeneWikii GINS2.
GenomeRNAii 51659.
NextBioi 55634.
PROi Q9Y248.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Y248.
Bgeei Q9Y248.
CleanExi HS_GINS2.
Genevestigatori Q9Y248.

Family and domain databases

InterProi IPR021151. GINS_complex.
IPR007257. GINS_Psf2.
IPR016906. GINS_Psf2_subgr.
[Graphical view ]
PANTHERi PTHR12772. PTHR12772. 1 hit.
Pfami PF05916. Sld5. 1 hit.
[Graphical view ]
PIRSFi PIRSF028998. GINS_Psf2_subgr. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Novel genes expressed in human dendritic cells."
    Li Y., Peng Y., Li N., Gu W., Han Z., Fu G., Chen Z.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Dendritic cell.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-180 AND SER-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (33 ANGSTROMS) IN COMPLEX WITH PSF1; PSF3 AND GINS4, SUBUNIT, MASS SPECTROMETRY OF GINS COMPLEX.
  16. "Crystal structure of the human GINS complex."
    Choi J.M., Lim H.S., Kim J.J., Song O.K., Cho Y.
    Genes Dev. 21:1316-1321(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH GINS1; GINS3 AND GINS4, SUBUNIT.
  17. "Structure of the human GINS complex and its assembly and functional interface in replication initiation."
    Kamada K., Kubota Y., Arata T., Shindo Y., Hanaoka F.
    Nat. Struct. Mol. Biol. 14:388-396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH GINS1; GINS3 AND GINS4, SUBUNIT.
  18. "Crystal structure of the GINS complex and functional insights into its role in DNA replication."
    Chang Y.P., Wang G., Bermudez V., Hurwitz J., Chen X.S.
    Proc. Natl. Acad. Sci. U.S.A. 104:12685-12690(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.36 ANGSTROMS) IN COMPLEX WITH GINS1; GINS3 AND GINS4, SUBUNIT, REGION.

Entry informationi

Entry nameiPSF2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y248
Secondary accession number(s): D3DUM5, Q6IAG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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