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Protein

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4

Gene

PIN4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.1 Publication
Isoform 2 binds to double-stranded DNA.1 Publication

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  • bent DNA binding Source: UniProtKB
  • DNA binding Source: UniProtKB-KW
  • double-stranded DNA binding Source: UniProtKB
  • peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • rRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS02381-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC:5.2.1.8)
Alternative name(s):
Parvulin-14
Short name:
Par14
Short name:
hPar14
Parvulin-17
Short name:
Par17
Short name:
hPar17
Peptidyl-prolyl cis-trans isomerase Pin4
Short name:
PPIase Pin4
Peptidyl-prolyl cis/trans isomerase EPVH
Short name:
hEPVH
Rotamase Pin4
Gene namesi
Name:PIN4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:8992. PIN4.

Subcellular locationi

Isoform 1 :
Isoform 2 :
  • Mitochondrion
  • Mitochondrion matrix

  • Note: Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • mitochondrial matrix Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleus Source: UniProtKB
  • preribosome Source: UniProtKB
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19S → A: Abolishes phosphorylation and reduces strongly nuclear localization. 1 Publication1
Mutagenesisi19S → E: Does not abolish nuclear localization and reduces DNA-binding ability. 1 Publication1

Organism-specific databases

DisGeNETi5303.
OpenTargetsiENSG00000102309.
PharmGKBiPA33324.

Chemistry databases

ChEMBLiCHEMBL4923.

Polymorphism and mutation databases

DMDMi20139299.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001934381 – 131Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4Add BLAST131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphoserine; by CK21 Publication1

Post-translational modificationi

Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.2 Publications
Isoform 2 is sumoylated with SUMO2 and SUMO3.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y237.
PaxDbiQ9Y237.
PeptideAtlasiQ9Y237.
PRIDEiQ9Y237.
TopDownProteomicsiQ9Y237-1. [Q9Y237-1]
Q9Y237-2. [Q9Y237-2]

PTM databases

iPTMnetiQ9Y237.
PhosphoSitePlusiQ9Y237.

Expressioni

Tissue specificityi

Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.3 Publications

Gene expression databases

BgeeiENSG00000102309.
CleanExiHS_PIN4.
ExpressionAtlasiQ9Y237. baseline and differential.
GenevisibleiQ9Y237. HS.

Organism-specific databases

HPAiHPA054483.
HPA064504.

Interactioni

Subunit structurei

Isoform 1 is found in pre-ribosomal ribonucleoprotein (pre-rRNP) complexes.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP72Q9P2093EBI-714599,EBI-739498
FAM9BQ8IZU03EBI-714599,EBI-10175124
FATE1Q969F03EBI-714599,EBI-743099
MAGEA11P43364-23EBI-714599,EBI-10178634
PNMA1Q8ND903EBI-714599,EBI-302345
ZBTB9Q96C003EBI-714599,EBI-395708

Protein-protein interaction databases

BioGridi111320. 33 interactors.
DIPiDIP-50838N.
IntActiQ9Y237. 16 interactors.
STRINGi9606.ENSP00000362773.

Chemistry databases

BindingDBiQ9Y237.

Structurei

Secondary structure

1131
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 47Combined sources11
Helixi48 – 59Combined sources12
Helixi64 – 71Combined sources8
Beta strandi73 – 75Combined sources3
Helixi76 – 78Combined sources3
Beta strandi81 – 86Combined sources6
Helixi92 – 99Combined sources8
Turni108 – 110Combined sources3
Beta strandi116 – 118Combined sources3
Beta strandi121 – 130Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EQ3NMR-A36-131[»]
1FJDNMR-A28-131[»]
3UI4X-ray0.80A36-131[»]
3UI5X-ray1.40A36-131[»]
3UI6X-ray0.89A36-131[»]
ProteinModelPortaliQ9Y237.
SMRiQ9Y237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y237.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 129PpiCPROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 41Necessary for association with the pre-rRNP complexesAdd BLAST41
Regioni1 – 25Necessary for nuclear localization and DNA-bindingAdd BLAST25

Domaini

The PPIase domain enhances mitochondrial targeting.

Sequence similaritiesi

Contains 1 PpiC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3258. Eukaryota.
COG0760. LUCA.
GeneTreeiENSGT00510000047029.
HOVERGENiHBG019150.
InParanoidiQ9Y237.
KOiK09579.
OMAiCEKEAKL.
OrthoDBiEOG091G0Z6N.
PhylomeDBiQ9Y237.
TreeFamiTF101102.

Family and domain databases

InterProiIPR000297. PPIase_PpiC.
[Graphical view]
PROSITEiPS50198. PPIC_PPIASE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: Q9Y237-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPKGKSGSG KAGKGGAASG SDSADKKAQG PKGGGNAVKV RHILCEKHGK
60 70 80 90 100
IMEAMEKLKS GMRFNEVAAQ YSEDKARQGG DLGWMTRGSM VGPFQEAAFA
110 120 130
LPVSGMDKPV FTDPPVKTKF GYHIIMVEGR K
Length:131
Mass (Da):13,810
Last modified:November 1, 1999 - v1
Checksum:i787C15BDB0701258
GO
Isoform 2 (identifier: Q9Y237-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPMAGLLKGLVRQLERFSVQQQASKM

Note: The first 25 amino acids are sufficient for mitochondrial targeting.2 Publications
Show »
Length:156
Mass (Da):16,608
Checksum:i8D4668D3ADB24740
GO
Isoform 3 (identifier: Q9Y237-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPMAGLLKGLVRQLERFSVQQQASKM
     80-131: GDLGWMTRGS...YHIIMVEGRK → IPSLQQHAGHHRDLRSTLISLVSYLQTTP

Note: No experimental confirmation available.
Show »
Length:133
Mass (Da):14,179
Checksum:iB217F63257D92D65
GO

Sequence cautioni

The sequence AAH05234 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH70288 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI04654 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI11395 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAG54532 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q9Y237-2)
Natural varianti16R → Q.2 PublicationsCorresponds to variant rs6525589dbSNPEnsembl.1
Natural varianti18S → R.2 PublicationsCorresponds to variant rs7058353dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0377541M → MPMAGLLKGLVRQLERFSVQ QQASKM in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_04612280 – 131GDLGW…VEGRK → IPSLQQHAGHHRDLRSTLIS LVSYLQTTP in isoform 3. 1 PublicationAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143096 mRNA. Translation: AAD27893.1.
AB009690 mRNA. Translation: BAA82320.1.
BX119917, AL135749 Genomic DNA. Translation: CAI39856.1.
BC005234 mRNA. Translation: AAH05234.2. Different initiation.
BC070288 mRNA. Translation: AAH70288.1. Different initiation.
BC093700 mRNA. Translation: AAH93700.1.
BC104653 mRNA. Translation: AAI04654.1. Different initiation.
BC111394 mRNA. Translation: AAI11395.1. Different initiation.
BC112281 mRNA. Translation: AAI12282.1.
AM420633 Genomic DNA. Translation: CAM12362.1.
AK127605 mRNA. Translation: BAG54532.1. Different initiation.
CCDSiCCDS14417.1. [Q9Y237-2]
CCDS55447.1. [Q9Y237-3]
RefSeqiNP_001164218.1. NM_001170747.1. [Q9Y237-3]
NP_006214.2. NM_006223.3. [Q9Y237-2]
UniGeneiHs.655623.

Genome annotation databases

EnsembliENST00000373669; ENSP00000362773; ENSG00000102309. [Q9Y237-2]
ENST00000423432; ENSP00000409154; ENSG00000102309. [Q9Y237-3]
GeneIDi5303.
KEGGihsa:5303.
UCSCiuc004eam.4. human. [Q9Y237-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143096 mRNA. Translation: AAD27893.1.
AB009690 mRNA. Translation: BAA82320.1.
BX119917, AL135749 Genomic DNA. Translation: CAI39856.1.
BC005234 mRNA. Translation: AAH05234.2. Different initiation.
BC070288 mRNA. Translation: AAH70288.1. Different initiation.
BC093700 mRNA. Translation: AAH93700.1.
BC104653 mRNA. Translation: AAI04654.1. Different initiation.
BC111394 mRNA. Translation: AAI11395.1. Different initiation.
BC112281 mRNA. Translation: AAI12282.1.
AM420633 Genomic DNA. Translation: CAM12362.1.
AK127605 mRNA. Translation: BAG54532.1. Different initiation.
CCDSiCCDS14417.1. [Q9Y237-2]
CCDS55447.1. [Q9Y237-3]
RefSeqiNP_001164218.1. NM_001170747.1. [Q9Y237-3]
NP_006214.2. NM_006223.3. [Q9Y237-2]
UniGeneiHs.655623.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EQ3NMR-A36-131[»]
1FJDNMR-A28-131[»]
3UI4X-ray0.80A36-131[»]
3UI5X-ray1.40A36-131[»]
3UI6X-ray0.89A36-131[»]
ProteinModelPortaliQ9Y237.
SMRiQ9Y237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111320. 33 interactors.
DIPiDIP-50838N.
IntActiQ9Y237. 16 interactors.
STRINGi9606.ENSP00000362773.

Chemistry databases

BindingDBiQ9Y237.
ChEMBLiCHEMBL4923.

PTM databases

iPTMnetiQ9Y237.
PhosphoSitePlusiQ9Y237.

Polymorphism and mutation databases

DMDMi20139299.

Proteomic databases

EPDiQ9Y237.
PaxDbiQ9Y237.
PeptideAtlasiQ9Y237.
PRIDEiQ9Y237.
TopDownProteomicsiQ9Y237-1. [Q9Y237-1]
Q9Y237-2. [Q9Y237-2]

Protocols and materials databases

DNASUi5303.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373669; ENSP00000362773; ENSG00000102309. [Q9Y237-2]
ENST00000423432; ENSP00000409154; ENSG00000102309. [Q9Y237-3]
GeneIDi5303.
KEGGihsa:5303.
UCSCiuc004eam.4. human. [Q9Y237-1]

Organism-specific databases

CTDi5303.
DisGeNETi5303.
GeneCardsiPIN4.
H-InvDBHIX0016866.
HGNCiHGNC:8992. PIN4.
HPAiHPA054483.
HPA064504.
MIMi300252. gene.
neXtProtiNX_Q9Y237.
OpenTargetsiENSG00000102309.
PharmGKBiPA33324.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3258. Eukaryota.
COG0760. LUCA.
GeneTreeiENSGT00510000047029.
HOVERGENiHBG019150.
InParanoidiQ9Y237.
KOiK09579.
OMAiCEKEAKL.
OrthoDBiEOG091G0Z6N.
PhylomeDBiQ9Y237.
TreeFamiTF101102.

Enzyme and pathway databases

BioCyciZFISH:HS02381-MONOMER.

Miscellaneous databases

ChiTaRSiPIN4. human.
EvolutionaryTraceiQ9Y237.
GeneWikiiPIN4.
GenomeRNAii5303.
PROiQ9Y237.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102309.
CleanExiHS_PIN4.
ExpressionAtlasiQ9Y237. baseline and differential.
GenevisibleiQ9Y237. HS.

Family and domain databases

InterProiIPR000297. PPIase_PpiC.
[Graphical view]
PROSITEiPS50198. PPIC_PPIASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIN4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y237
Secondary accession number(s): A8E0G6
, B3KXM0, F5H1P5, Q0D2H3, Q3MHV0, Q52M21, Q5HYW6, Q6IRW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.