Reviewed,
UniProtKB/Swiss-Prot Q9Y230 (RUVB2_HUMAN)
Last modified
July 7, 2009.
Version 105.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
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Names and origin
| Protein names | Recommended name: RuvB-like 2 EC=3.6.1.- Alternative name(s): 48 kDa TATA box-binding protein-interacting protein Short name=48 kDa TBP-interacting protein TIP49b Repressing pontin 52 Short name=Reptin 52 51 kDa erythrocyte cytosolic protein Short name=ECP-51 TIP60-associated protein 54-beta Short name=TAP54-beta INO80 complex subunit J | ||||||
| Gene names |
| ||||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 463 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. |
| Subunit structure | Forms a multimeric complex with RUVBL1. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and EAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Ref.2 Ref.5 Ref.15 Ref.19 |
| Subcellular location | Nucleus matrix. Nucleus › nucleoplasm. Cytoplasm. Membrane. Note: Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. |
| Tissue specificity | Ubiquitously expressed. Highly expressed in testis and thymus. |
| Domain | The C-terminal domain is required for association with ATF2. |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.18 |
| Sequence similarities | Belongs to the ruvB family. |
| Sequence caution | The sequence AAD34041.1 differs from that shown. Reason: Frameshift at position 401. The sequence AAH08355.1 differs from that shown. Reason: Frameshift at position 191. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| BCAS1 | O75363 | 1 | EBI-352939,EBI-1042966 | |
| C2orf44 | Q9H6R7 | 1 | EBI-352939,EBI-346906 | |
| DNASE2 | O00115 | 1 | EBI-352939,EBI-1051412 | |
| DPCD | Q9BVM2 | 1 | EBI-352939,EBI-749988 | |
| EXOSC10 | Q01780 | 1 | EBI-352939,EBI-358236 | |
| IKBKE | Q14164 | 1 | EBI-352939,EBI-307369 | |
| NDRG1 | Q92597 | 1 | EBI-352939,EBI-716486 | |
| NEK6 | Q9HC98 | 1 | EBI-352939,EBI-740364 | |
| NME2 | P22392 | 1 | EBI-352939,EBI-713693 | |
| PLOD2 | O00469 | 1 | EBI-352939,EBI-1047459 | |
| RGS20 | O76081 | 1 | EBI-352939,EBI-1052678 | |
| RIOK3 | O14730 | 1 | EBI-352939,EBI-1047061 | |
| RPS11 | P62280 | 1 | EBI-352939,EBI-1047710 | |
| RUVBL1 | Q9Y265 | 4 | EBI-352939,EBI-353675 | |
| SSSCA1 | O60232 | 1 | EBI-352939,EBI-741415 | |
| UQCRC1 | P31930 | 1 | EBI-352939,EBI-1052596 | |
| USP7 | Q93009 | 1 | EBI-352939,EBI-302474 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||
Molecule processing | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.11 Ref.12 | ||||||||||||||||||||
| Chain | 2 – 463 | 462 | RuvB-like 2 | PRO_0000165644 | |||||||||||||||||||
Regions | |||||||||||||||||||||||
| Nucleotide binding | 77 – 84 | 8 | ATP | ||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.12 | ||||||||||||||||||||
| Modified residue | 220 | 1 | Phosphoserine Ref.18 | ||||||||||||||||||||
Experimental info | |||||||||||||||||||||||
| Sequence conflict | 214 | 1 | D → N in AAD34041. Ref.6 | ||||||||||||||||||||
| Sequence conflict | 257 – 258 | 2 | FL → YV AA sequence Ref.5 | ||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||
| Beta strand | 135 – 147 | 13 | |||||||||||||||||||||
| Beta strand | 151 – 155 | 5 | |||||||||||||||||||||
| Beta strand | 157 – 163 | 7 | |||||||||||||||||||||
| Beta strand | 165 – 173 | 9 | |||||||||||||||||||||
| Helix | 176 – 183 | 8 | |||||||||||||||||||||
| Beta strand | 190 – 195 | 6 | |||||||||||||||||||||
| Turn | 196 – 199 | 4 | |||||||||||||||||||||
| Beta strand | 200 – 205 | 6 | |||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins." Salzer U., Kubicek M., Prohaska R. Biochim. Biophys. Acta 1446:365-370(1999) [PubMed: 10524211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 72-83; 131-144; 212-223; 369-372 AND 440-457. Tissue: Bone marrow. |
| [2] | "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a." Kanemaki M., Kurokawa Y., Matsu-ura T., Makino Y., Masani A., Okazaki K., Morishita T., Tamura T.-A. J. Biol. Chem. 274:22437-22444(1999) [PubMed: 10428817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, INTERACTION WITH RUVBL1. Tissue: Liver. |
| [3] | "Human TIP49b/RUVBL2 gene: genomic structure, expression pattern, physical link to the human CGB/LHB gene cluster on chromosome 19q13.3." Parfait B., Giovangrandi Y., Asheuer M., Laurendeau I., Olivi M., Vodovar N., Vidaud D., Vidaud M., Bieche I. Ann. Genet. 43:69-74(2000) [PubMed: 10998447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland. |
| [4] | "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity." Bauer A., Chauvet S., Huber O., Usseglio F., Rothbaecher U., Aragnol D., Kemler R., Pradel J. EMBO J. 19:6121-6130(2000) [PubMed: 11080158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver. |
| [5] | "An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc." Wood M.A., McMahon S.B., Cole M.D. Mol. Cell 5:321-330(2000) [PubMed: 10882073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 73-83; 116-124; 237-253 AND 254-269, INTERACTION WITH MYC. |
| [6] | "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [7] | "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A.Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis. |
| [8] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo. |
| [9] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [10] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Lung. |
| [11] | "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Tissue: Platelet. |
| [12] | Bienvenut W.V., Heiserich L., Gottlieb E. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-18; 30-53; 55-64; 72-83; 116-124; 165-177; 237-269; 335-368; 393-400 AND 418-438, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Colon carcinoma. |
| [13] | "Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis." Ikura T., Ogryzko V.V., Grigoriev M., Groisman R., Wang J., Horikoshi M., Scully R., Qin J., Nakatani Y. Cell 102:463-473(2000) [PubMed: 10966108] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-40; 185-197; 354-365 AND 417-427, IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY. |
| [14] | "Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex." Cai Y., Jin J., Tomomori-Sato C., Sato S., Sorokina I., Parmely T.J., Conaway R.C., Conaway J.W. J. Biol. Chem. 278:42733-42736(2003) [PubMed: 12963728] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-40; 72-83; 165-177; 187-197; 254-269; 354-365; 418-438 AND 445-463, IDENTIFICATION IN NUA4 COMPLEX, MASS SPECTROMETRY. |
| [15] | "TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage." Cho S.-G., Bhoumik A., Broday L., Ivanov V., Rosenstein B., Ronai Z. Mol. Cell. Biol. 21:8398-8413(2001) [PubMed: 11713276] [Abstract] Cited for: INTERACTION WITH ATF2. |
| [16] | "Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans." Doyon Y., Selleck W., Lane W.S., Tan S., Cote J. Mol. Cell. Biol. 24:1884-1896(2004) [PubMed: 14966270] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN NUA4 COMPLEX, IDENTIFICATION IN A NUA4-RELATED SRCAP-CONTAINING COMPLEX. |
| [17] | "A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex." Jin J., Cai Y., Yao T., Gottschalk A.J., Florens L., Swanson S.K., Gutierrez J.L., Coleman M.K., Workman J.L., Mushegian A., Washburn M.P., Conaway R.C., Conaway J.W. J. Biol. Chem. 280:41207-41212(2005) [PubMed: 16230350] [Abstract] Cited for: IDENTIFICATION IN INO80 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY. |
| [18] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, MASS SPECTROMETRY. |
| [19] | "Transcriptional activation of histone genes requires NPAT-dependent recruitment of TRRAP-Tip60 complex to histone promoters during the G1/S phase transition." DeRan M., Pulvino M., Greene E., Su C., Zhao J. Mol. Cell. Biol. 28:435-447(2008) [PubMed: 17967892] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH NPAT. |
| [20] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [21] | "Solution structure of RSGI ruh-039, a fragment of C-terminal domain of RuvB-like 2 from human cDNA." Mycobacterium tuberculosis structural genomics consortium (TB) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 132-213. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Y18417 mRNA. Translation: CAB46270.1. AB024301 mRNA. Translation: BAA76708.1. AF155138 mRNA. Translation: AAD38073.1. AF124607 mRNA. Translation: AAF87087.1. AF151804 mRNA. Translation: AAD34041.1. Frameshift. AL136743 mRNA. Translation: CAB66677.1. AK074542 mRNA. Translation: BAC11048.1. CR533507 mRNA. Translation: CAG38538.1. BC000428 mRNA. Translation: AAH00428.1. BC004531 mRNA. Translation: AAH04531.1. BC008355 mRNA. Translation: AAH08355.1. Frameshift. | |||||||||||||
| IPI | IPI00009104. | ||||||||||||
| PIR | T46313. | ||||||||||||
| RefSeq | NP_006657.1. | ||||||||||||
| UniGene | Hs.515846 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:28153N. | ||||||||||||
| IntAct | Q9Y230. 74 interactions. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q9Y230. | ||||||||||||
2-D gel databases | |||||||||||||
| REPRODUCTION-2DPAGE | IPI00009104. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | Q9Y230. | ||||||||||||
| PRIDE | Q9Y230. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000183207. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 10856. | ||||||||||||
| KEGG | hsa:10856. | ||||||||||||
| UCSC | uc002plr.1. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| GeneCards | GC19P054188. | ||||||||||||
| H-InvDB | HIX0015312. | ||||||||||||
| HGNC | HGNC:10475. RUVBL2. | ||||||||||||
| HPA | CAB012432. | ||||||||||||
| MIM | 604788. gene. | ||||||||||||
| PharmGKB | PA34888. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q9Y230. | ||||||||||||
| HOVERGEN | Q9Y230. | ||||||||||||
| OMA | Q9Y230. TTDMETI. | ||||||||||||
Gene expression databases | |||||||||||||
| Bgee | Q9Y230. | ||||||||||||
| CleanEx | HS_RUVBL2. | ||||||||||||
| GermOnline | ENSG00000183207. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR004504. DNA_repair_RadA. IPR010339. TIP49_C. [Graphical view] | ||||||||||||
| Pfam | PF00004. AAA. 1 hit. PF06068. TIP49. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR01874. DNAREPAIRADA. | ||||||||||||
| SMART | SM00382. AAA. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 41211. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | RUVB2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9Y230 Secondary accession number(s): Q6FIB9, Q6PK27, Q9Y361 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


