Q9Y228 (T3JAM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TRAF3-interacting JNK-activating modulator

Alternative name(s):
TRAF3-interacting protein 3

Gene names

Name:	TRAF3IP3
Synonyms:	T3JAM

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as an adapter molecule that regulates TRAF3-mediated JNK activation By similarity.
Subunit structure	Binds to the isoleucine zipper of TRAF3 via its coiled-coil domain By similarity.
Subcellular location	Membrane; Single-pass type IV membrane protein Potential.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MOB4	Q9Y3A3	2	EBI-765817,EBI-713935
SIKE1	Q9BRV8	2	EBI-765817,EBI-1773646
STK24	Q9Y6E0	2	EBI-765817,EBI-740175
STRIP1	Q5VSL9	2	EBI-765817,EBI-1773588
STRN	O43815	2	EBI-765817,EBI-1046642
STRN3	Q13033	2	EBI-765817,EBI-1053857

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y228-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q9Y228-2)

The sequence of this isoform differs from the canonical sequence as follows:
     95-114: Missing.

Isoform 3 (identifier: Q9Y228-3)

The sequence of this isoform differs from the canonical sequence as follows:
     352-353: GA → IN
     354-551: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	TRAF3-interacting JNK-activating modulator		PRO_0000072403
Regions
	Topological domain	1 – 526	526	Cytoplasmic Potential
	Transmembrane	527 – 544	18	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	545 – 551	7	Extracellular Potential
	Coiled coil	240 – 436	197	Potential
	Coiled coil	464 – 506	43	Potential
Amino acid modifications
	Cross-link	267		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5
Natural variations
	Alternative sequence	95 – 114	20	Missing in isoform 2.		VSP_017271
	Alternative sequence	352 – 353	2	GA → IN in isoform 3.		VSP_017272
	Alternative sequence	354 – 551	198	Missing in isoform 3.		VSP_017273
	Natural variant	373	1	Q → E. Ref.3 Ref.4 Corresponds to variant rs669694 [ dbSNP | Ensembl ].		VAR_024283
	Natural variant	529	1	P → S in a colorectal cancer sample; somatic mutation. Ref.6		VAR_035664

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: EA2DFF45A8D6ED19	FASTA	551	63,626
	10 20 30 40 50 60 MISPDPRPSP GLARWAESYE AKCERRQEIR ESRRCRPNVT TCRQVGKTLR IQQREQLQRA 70 80 90 100 110 120 RLQQFFRRRN LELEEKGKAQ HPQAREQGPS RRPGQVTVLK EPLSCARRIS SPREQVTGTS 130 140 150 160 170 180 SEVFPAQHPP PSGICRDLSD HLSSQAGGLP PQDTPIKKPP KHHRGTQTKA EGPTIKNDAS 190 200 210 220 230 240 QQTNYGVAVL DKEIIQLSDY LKEALQRELV LKQKMVILQD LLSTLIQASD SSWKGQLNED 250 260 270 280 290 300 KLKGKLRSLE NQLYTCTQKY SPWGMKKVLL EMEDQKNSYE QKAKESLQKV LEEKMNAEQQ 310 320 330 340 350 360 LQSTQRSLAL AEQKCEEWRS QYEALKEDWR TLGTQHRELE SQLHVLQSKL QGADSRDLQM 370 380 390 400 410 420 NQALRFLENE HQQLQAKIEC LQGDRDLCSL DTQDLQDQLK RSEAEKLTLV TRVQQLQGLL 430 440 450 460 470 480 QNQSLQLQEQ EKLLTKKDQA LPVWSPKSFP NEVEPEGTGK EKDWDLRDQL QKKTLQLQAK 490 500 510 520 530 540 EKECRELHSE LDNLSDEYLS CLRKLQHCRE ELNQSQQLPP RRQCGRWLPV LMVVIAAALA 550 VFLANKDNLM I
	Isoform 2 [UniParc]. Checksum: 3ABAB20EEB018E30	FASTA	531	61,375
	10 20 30 40 50 60 MISPDPRPSP GLARWAESYE AKCERRQEIR ESRRCRPNVT TCRQVGKTLR IQQREQLQRA 70 80 90 100 110 120 RLQQFFRRRN LELEEKGKAQ HPQAREQGPS RRPGQVTGTS SEVFPAQHPP PSGICRDLSD 130 140 150 160 170 180 HLSSQAGGLP PQDTPIKKPP KHHRGTQTKA EGPTIKNDAS QQTNYGVAVL DKEIIQLSDY 190 200 210 220 230 240 LKEALQRELV LKQKMVILQD LLSTLIQASD SSWKGQLNED KLKGKLRSLE NQLYTCTQKY 250 260 270 280 290 300 SPWGMKKVLL EMEDQKNSYE QKAKESLQKV LEEKMNAEQQ LQSTQRSLAL AEQKCEEWRS 310 320 330 340 350 360 QYEALKEDWR TLGTQHRELE SQLHVLQSKL QGADSRDLQM NQALRFLENE HQQLQAKIEC 370 380 390 400 410 420 LQGDRDLCSL DTQDLQDQLK RSEAEKLTLV TRVQQLQGLL QNQSLQLQEQ EKLLTKKDQA 430 440 450 460 470 480 LPVWSPKSFP NEVEPEGTGK EKDWDLRDQL QKKTLQLQAK EKECRELHSE LDNLSDEYLS 490 500 510 520 530 CLRKLQHCRE ELNQSQQLPP RRQCGRWLPV LMVVIAAALA VFLANKDNLM I
	Isoform 3 [UniParc]. Checksum: 5405FE9F07EE18F7	FASTA	353	40,674
	10 20 30 40 50 60 MISPDPRPSP GLARWAESYE AKCERRQEIR ESRRCRPNVT TCRQVGKTLR IQQREQLQRA 70 80 90 100 110 120 RLQQFFRRRN LELEEKGKAQ HPQAREQGPS RRPGQVTVLK EPLSCARRIS SPREQVTGTS 130 140 150 160 170 180 SEVFPAQHPP PSGICRDLSD HLSSQAGGLP PQDTPIKKPP KHHRGTQTKA EGPTIKNDAS 190 200 210 220 230 240 QQTNYGVAVL DKEIIQLSDY LKEALQRELV LKQKMVILQD LLSTLIQASD SSWKGQLNED 250 260 270 280 290 300 KLKGKLRSLE NQLYTCTQKY SPWGMKKVLL EMEDQKNSYE QKAKESLQKV LEEKMNAEQQ 310 320 330 340 350 LQSTQRSLAL AEQKCEEWRS QYEALKEDWR TLGTQHRELE SQLHVLQSKL QIN

References

[1]	Rhodes S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1).
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-373.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT GLU-373. Tissue: Blood and Brain.
[5]	"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D. J. Proteome Res. 6:298-305(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-267, MASS SPECTROMETRY. Tissue: Lung adenocarcinoma.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-529.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL049667 mRNA. Translation: CAB41242.1. AL022398 Genomic DNA. Translation: CAI95687.1. CH471100 Genomic DNA. Translation: EAW93444.1. BC110302 mRNA. Translation: AAI10303.1. BC130417 mRNA. Translation: AAI30418.1. BC144139 mRNA. Translation: AAI44140.1.
IPI	IPI00019248. IPI00645440. IPI00719170.
RefSeq	NP_079504.2. NM_025228.2.
UniGene	Hs.147434.
3D structure databases
ProteinModelPortal	Q9Y228.
SMR	Q9Y228. Positions 477-514.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y228. 28 interactions.
MINT	MINT-6768551.
STRING	9606.ENSP00000010338.
PTM databases
PhosphoSite	Q9Y228.
Polymorphism databases
DMDM	88984949.
Proteomic databases
PaxDb	Q9Y228.
PRIDE	Q9Y228.
Protocols and materials databases
DNASU	80342.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000010338; ENSP00000010338; ENSG00000009790. ENST00000367024; ENSP00000355991; ENSG00000009790. ENST00000367025; ENSP00000355992; ENSG00000009790. ENST00000367026; ENSP00000355993; ENSG00000009790. ENST00000478359; ENSP00000417665; ENSG00000009790.
GeneID	80342.
KEGG	hsa:80342.
UCSC	uc001hhm.2. human. uc001hhn.3. human. uc001hho.3. human.
Organism-specific databases
CTD	80342.
GeneCards	GC01P209929.
H-InvDB	HIX0159943.
HGNC	HGNC:30766. TRAF3IP3.
HPA	HPA040796.
MIM	608255. gene.
neXtProt	NX_Q9Y228.
PharmGKB	PA142670711.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG81914.
HOGENOM	HOG000070108.
HOVERGEN	HBG054704.
InParanoid	Q9Y228.
OMA	CERRQET.
OrthoDB	EOG402WS3.
Gene expression databases
ArrayExpress	Q9Y228.
Bgee	Q9Y228.
CleanEx	HS_TRAF3IP3.
Genevestigator	Q9Y228.
GermOnline	ENSG00000009790. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	TRAF3IP3. human.
GenomeRNAi	80342.
NextBio	70918.
SOURCE	Search...

Entry information

Entry name

T3JAM_HUMAN

Accession

Primary (citable) accession number: Q9Y228
Secondary accession number(s): A1L464 , A6NIU9, Q2YDB5, Q4VY06, Q7Z706

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	February 21, 2006
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.