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Protein

3-phosphoinositide-dependent protein kinase 1

Gene

pdk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the daf-2/insulin receptor-like transduction pathway, which controls longevity and prevents developmental arrest at the dauer stage (PubMed:10364160). Phosphorylates and activates sgk-1, akt-1 and akt-2 (PubMed:15068796).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98ATPBy similarity1
Binding sitei158ATPBy similarity1
Active sitei197Proton acceptorPROSITE-ProRule annotation1
Binding sitei201ATP; via carbonyl oxygenBy similarity1
Binding sitei215ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi79 – 81ATPBy similarity3
Nucleotide bindingi152 – 154ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • dauer larval development Source: WormBase
  • intracellular signal transduction Source: GO_Central
  • learning or memory Source: WormBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein phosphorylation Source: WormBase
  • regulation of chemotaxis Source: WormBase
  • regulation of synaptic growth at neuromuscular junction Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-1257604. PIP3 activates AKT signaling.
R-CEL-165158. Activation of AKT2.
R-CEL-202424. Downstream TCR signaling.
R-CEL-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-CEL-389357. CD28 dependent PI3K/Akt signaling.
R-CEL-389513. CTLA4 inhibitory signaling.
R-CEL-392451. G beta:gamma signalling through PI3Kgamma.
R-CEL-444257. RSK activation.
R-CEL-5218920. VEGFR2 mediated vascular permeability.
R-CEL-5218921. VEGFR2 mediated cell proliferation.
R-CEL-5625740. RHO GTPases activate PKNs.
R-CEL-6804757. Regulation of TP53 Degradation.
SignaLinkiQ9Y1J3.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoinositide-dependent protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
Pdk-class protein kinase 1
Gene namesi
Name:pdk-1
ORF Names:H42K12.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiH42K12.1a; CE28739; WBGene00003965; pdk-1.
H42K12.1b; CE28740; WBGene00003965; pdk-1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi297G → R in sa680; developmental arrest at dauer stage, extended life span, increased body size, low fertility, and defects in egg-laying and social behavior. Fails to avoid NaCl after exposure to NaCl under starvation conditions. Restores normal AIY interneuron neurite outgrowth in a daf-18 (mg198) background mutant. 3 Publications1
Mutagenesisi305A → V in mg142; no obvious phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865031 – 6363-phosphoinositide-dependent protein kinase 1Add BLAST636

Proteomic databases

EPDiQ9Y1J3.
PaxDbiQ9Y1J3.
PeptideAtlasiQ9Y1J3.

PTM databases

iPTMnetiQ9Y1J3.

Expressioni

Developmental stagei

Expressed in late stage embryos and throughout life. At L1, expressed in neurons, intestinal cells and hypodermal cells. In adults, expressed in the somatic gonad.1 Publication

Gene expression databases

BgeeiWBGene00003965.

Interactioni

Subunit structurei

Interacts directly with sgk-1, akt-1 and akt-2.1 Publication

Protein-protein interaction databases

BioGridi45427. 2 interactors.
DIPiDIP-27322N.
IntActiQ9Y1J3. 3 interactors.
MINTiMINT-1039329.
STRINGi6239.H42K12.1b.

Structurei

3D structure databases

ProteinModelPortaliQ9Y1J3.
SMRiQ9Y1J3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 364Protein kinasePROSITE-ProRule annotationAdd BLAST296

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 149PIF-pocketBy similarityAdd BLAST50

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili550 – 631Sequence analysisAdd BLAST82

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0592. Eukaryota.
ENOG410XRT8. LUCA.
GeneTreeiENSGT00550000074819.
HOGENOMiHOG000233026.
InParanoidiQ9Y1J3.
KOiK06276.
OMAiHAYIPAT.
OrthoDBiEOG091G06CX.
PhylomeDBiQ9Y1J3.

Family and domain databases

CDDicd01262. PH_PDK1. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR033931. PDK1-typ_PH.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF14593. PH_3. 1 hit.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF50729. SSF50729. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9Y1J3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDLTPTNTS LDTTTTNNDT TSDREAAPTT LNLTPTASES ENSLSPVTAE
60 70 80 90 100
DLIAKSIKEG CPKRTSNDFM FLQSMGEGAY SQVFRCREVA TDAMFAVKVL
110 120 130 140 150
QKSYLNRHQK MDAIIREKNI LTYLSQECGG HPFVTQLYTH FHDQARIYFV
160 170 180 190 200
IGLVENGDLG ESLCHFGSFD MLTSKFFASE ILTGLQFLHD NKIVHRDMKP
210 220 230 240 250
DNVLIQKDGH ILITDFGSAQ AFGGLQLSQE GFTDANQASS RSSDSGSPPP
260 270 280 290 300
TRFYSDEEVP EENTARRTTF VGTALYVSPE MLADGDVGPQ TDIWGLGCIL
310 320 330 340 350
FQCLAGQPPF RAVNQYHLLK RIQELDFSFP EGFPEEASEI IAKILVRDPS
360 370 380 390 400
TRITSQELMA HKFFENVDWV NIANIKPPVL HAYIPATFGE PEYYSNIGPV
410 420 430 440 450
EPGLDDRALF RLMNLGNDAS ASQPSTFRPS NVEHRGDPFV SEIAPRANSE
460 470 480 490 500
AEKNRAARAQ KLEEQRVKNP FHIFTNNSLI LKQGYLEKKR GLFARRRMFL
510 520 530 540 550
LTEGPHLLYI DVPNLVLKGE VPWTPCMQVE LKNSGTFFIH TPNRVYYLFD
560 570 580 590 600
LEKKADEWCK AINDVRKRYS VTIEKTFNSA MRDGTFGSIY GKKKSRKEMM
610 620 630
REQKALRRKQ EKEEKKALKA EQVSKKLSMQ MDKKSP
Length:636
Mass (Da):71,917
Last modified:November 1, 1999 - v1
Checksum:i5885237039D80AF2
GO
Isoform b (identifier: Q9Y1J3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-260: Missing.
     427-428: Missing.

Show »
Length:632
Mass (Da):71,417
Checksum:iCF302D403142176A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017049259 – 260Missing in isoform b. 1 Publication2
Alternative sequenceiVSP_017050427 – 428Missing in isoform b. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130406 mRNA. Translation: AAD42307.1.
AF130407 mRNA. Translation: AAD42308.1.
FO080926 Genomic DNA. Translation: CCD67851.1.
FO080926 Genomic DNA. Translation: CCD67852.1.
RefSeqiNP_001024742.1. NM_001029571.2. [Q9Y1J3-2]
NP_001024743.1. NM_001029572.2. [Q9Y1J3-1]
UniGeneiCel.8028.

Genome annotation databases

EnsemblMetazoaiH42K12.1b; H42K12.1b; WBGene00003965. [Q9Y1J3-1]
GeneIDi180475.
KEGGicel:CELE_H42K12.1.
UCSCiH42K12.1b. c. elegans. [Q9Y1J3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDPK1_CAEEL
AccessioniPrimary (citable) accession number: Q9Y1J3
Secondary accession number(s): Q9UA36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1999
Last modified: October 25, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families