Q9Y194 (SGPL_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine-1-phosphate lyase
Short name=S1PL
Short name=SP-lyase
Short name=SPL
EC=4.1.2.27

Alternative name(s):
Sphingosine-1-phosphate aldolase

Gene names

Name:	spl-1
ORF Names:	Y66H1B.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine By similarity. Essential for normal development, growth and reproduction.
Catalytic activity	Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Tissue specificity	High expression found in the intestinal cells and faint expression in the head.
Developmental stage	Found in the majority of L1 larvae and in all L2, L3 and L4 larvae and adults showing medium to high expression. Highest levels found in older adults.
Sequence similarities	Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carboxylic acid metabolic process Inferred from electronic annotation. Source: InterPro determination of adult lifespan Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro sphinganine-1-phosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Sphingosine-1-phosphate lyase		PRO_0000147015
Regions
	Topological domain	1 – 31	31	Lumenal Potential
	Transmembrane	32 – 52	21	Helical; Signal-anchor for type III membrane protein; Potential
	Topological domain	53 – 552	500	Cytoplasmic Potential
Amino acid modifications
	Modified residue	347	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y194 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D12FFE7EBE9D3CFC	FASTA	552	61,097
	10 20 30 40 50 60 MDSVKHTTEI IVDLTKMHYH MINDRLSRYD PVVLVLAAFG GTLVYTKVVH LYRKSEDPIL 70 80 90 100 110 120 KRMGAYVFSL LRKLPAVRDK IEKELAAEKP KLIESIHKDD KDKQFISTLP IAPLSQDSIM 130 140 150 160 170 180 ELAKKYEDYN TFNIDGGRVS GAVYTDRHAE HINLLGKIYE KYAFSNPLHP DVFPGARKME 190 200 210 220 230 240 AELIRMVLNL YNGPEDSSGS VTSGGTESII MACFSYRNRA HSLGIEHPVI LACKTAHAAF 250 260 270 280 290 300 DKAAHLCGMR LRHVPVDSDN RVDLKEMERL IDSNVCMLVG SAPNFPSGTI DPIPEIAKLG 310 320 330 340 350 360 KKYGIPVHVD ACLGGFMIPF MNDAGYLIPV FDFRNPGVTS ISCDTHKYGC TPKGSSIVMY 370 380 390 400 410 420 RSKELHHFQY FSVADWCGGI YATPTIAGSR AGANTAVAWA TLLSFGRDEY VRRCAQIVKH 430 440 450 460 470 480 TRMLAEKIEK IKWIKPYGKS DVSLVAFSGN GVNIYEVSDK MMKLGWNLNT LQNPAAIHIC 490 500 510 520 530 540 LTINQANEEV VNAFAVDLEK ICEELAAKGE QKADSGMAAM YGMAAQVPKS VVDEVIALYI 550 DATYSAPPST SN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sphingosine phosphate lyase expression is essential for normal development in Caenorhabditis elegans." Mendel J., Heinecke K., Fyrst H., Saba J.D. J. Biol. Chem. 278:22341-22349(2003) [PubMed: 12682045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF144639 mRNA. Translation: AAD44756.1. FO081175 Genomic DNA. Translation: CCD69676.1.
PIR	T33760.
RefSeq	NP_499913.1. NM_067512.4.
UniGene	Cel.5572.
3D structure databases
ProteinModelPortal	Q9Y194.
SMR	Q9Y194. Positions 105-545.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y194. 1 interaction.
STRING	Q9Y194.
Proteomic databases
PRIDE	Q9Y194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y66H1B.4; Y66H1B.4; Y66H1B.4.
GeneID	176857.
KEGG	cel:Y66H1B.4.
UCSC	Y66H1B.4. c. elegans.
Organism-specific databases
CTD	176857.
WormBase	Y66H1B.4; CE20348; WBGene00004981; spl-1.
Phylogenomic databases
eggNOG	meNOG04015.
GeneTree	EMGT00050000011766.
HOGENOM	HBG657947.
InParanoid	Q9Y194.
OMA	IIAACWA.
PhylomeDB	Q9Y194.
Gene expression databases
ArrayExpress	Q9Y194.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
KO	K01634.
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	894318.

Entry information

Entry name

SGPL_CAEEL

Accession

Primary (citable) accession number: Q9Y194

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	November 1, 1999
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project