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Protein

Exostosin-2

Gene

Ext2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Plays a central role in diffusion of morphogens hedgehog (hh), wingless (wg) and Decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of Hh, Dpp and wg morphogens.3 Publications

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Pathwayi: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Pathwayi: heparin biosynthesis

This protein is involved in the pathway heparin biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparin biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei489SubstrateBy similarity1
Metal bindingi539Manganese; catalyticBy similarity1
Binding sitei568SubstrateBy similarity1
Active sitei627By similarity1

GO - Molecular functioni

GO - Biological processi

  • chondroitin sulfate biosynthetic process Source: FlyBase
  • decapentaplegic signaling pathway Source: FlyBase
  • glycosaminoglycan biosynthetic process Source: FlyBase
  • heparan sulfate proteoglycan biosynthetic process Source: FlyBase
  • heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: FlyBase
  • heparin biosynthetic process Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • N-acetylglucosamine metabolic process Source: UniProtKB
  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • positive regulation of decapentaplegic signaling pathway Source: UniProtKB
  • positive regulation of smoothened signaling pathway Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
Biological processWnt signaling pathway
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.225 1994
ReactomeiR-DME-2022928 HS-GAG biosynthesis
SignaLinkiQ9Y169
UniPathwayiUPA00378
UPA00756
UPA00862

Protein family/group databases

CAZyiGT47 Glycosyltransferase Family 47
GT64 Glycosyltransferase Family 64

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-2 (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Protein sister of tout-velu
Gene namesi
Name:Ext2
Synonyms:DEXT2, sotv
ORF Names:CG33038
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0029175 Ext2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini47 – 717LumenalSequence analysisAdd BLAST671

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496651 – 717Exostosin-2Add BLAST717

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi574N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi625 ↔ 675By similarity
Glycosylationi636N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Y169
PRIDEiQ9Y169

Expressioni

Tissue specificityi

In wing imaginal disk, it is ubiquitously expressed.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0029175
GenevisibleiQ9Y169 DM

Interactioni

Subunit structurei

Interacts with sau.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ttvQ9V7303EBI-142791,EBI-166374

Protein-protein interaction databases

BioGridi533698, 3 interactors
IntActiQ9Y169, 3 interactors
STRINGi7227.FBpp0099952

Structurei

3D structure databases

ProteinModelPortaliQ9Y169
SMRiQ9Y169
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni516 – 521Substrate bindingBy similarity6
Regioni537 – 539Substrate bindingBy similarity3
Regioni623 – 627Substrate bindingBy similarity5
Regioni661 – 672Substrate bindingBy similarityAdd BLAST12

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1021 Eukaryota
ENOG410XTFH LUCA
GeneTreeiENSGT00550000074496
InParanoidiQ9Y169
KOiK02367
OMAiSKLLVVW
OrthoDBiEOG091G02GW
PhylomeDBiQ9Y169

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004263 Exostosin
IPR027673 Exostosin-2
IPR015338 EXT_C
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR11062:SF128 PTHR11062:SF128, 1 hit
PfamiView protein in Pfam
PF03016 Exostosin, 1 hit
PF09258 Glyco_transf_64, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Y169-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKIKNLLSF VTQSRAISHT NPREHILNCL TYGLLVIVAL CAGFLLWDLS
60 70 80 90 100
SSPRDGFFHG KRDSHTLILD LEHIQELAVN PEAEQRARNV NCTFWDCLNI
110 120 130 140 150
YKCEHDRLKV YIYPLQEFVD EQSDKTATTL SSEYFQILEA VLKSRYYTSN
160 170 180 190 200
PNEACLFLPS LDLLNQNVFD KHLAGAALAS LDFWDRGANH IIFNMLPGGA
210 220 230 240 250
PSYNTVLDVN TDNAIIFGGG FDSWSYRPGF DVAIPVWSPR LVRQHAHATA
260 270 280 290 300
QRKFLLVVAQ LNILPRFVRT LRELSLAHSE QLLLLGACEN LDLTMRCPLS
310 320 330 340 350
QHHKSLEYPR LLSRGKFCLL GRSLRMGQPD LVEIMSQHCI PVIAVDNYVL
360 370 380 390 400
PFEDVIDWSL ASVRIRENEL HSVMQKLKAI SSVKIVEMQK QVQWLFSKYF
410 420 430 440 450
KDLKTVTLTA LEVLESRIFP LRARSSRQWN TIDTNARSTF NPLFLPSLAP
460 470 480 490 500
KSQGFTAVIL TYDRVESLFL LIQKLAVVPS LQSILVIWNN QKKSPPHLST
510 520 530 540 550
FPSISKPLKI RQTKENKLSN RFYPYPEIET EAILTIDDDI IMLTTDELDF
560 570 580 590 600
GYEVWREFPD HIVGFPSRIH VWENVTMRWH YESEWTNQIS MVLTGAAFHH
610 620 630 640 650
KYWSHMYTHA MPGDIKDWVD EHMNCEDIAM NFLVANITNN PPIKVTPRKK
660 670 680 690 700
FKCPECTNTE MLSADLNHMR ERSACIDRFS KIYGRMPLRT VEFRADPVLF
710
RDNFPDKLKR YNDIGSL
Length:717
Mass (Da):82,726
Last modified:November 1, 1999 - v1
Checksum:iA3894493C16EF7DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA Translation: AAO41379.1
AF145598 mRNA Translation: AAD38573.1
BT003544 mRNA Translation: AAO39548.1
RefSeqiNP_725536.1, NM_166150.3
UniGeneiDm.20855

Genome annotation databases

EnsemblMetazoaiFBtr0100513; FBpp0099953; FBgn0029175
GeneIDi3772101
KEGGidme:Dmel_CG8433
UCSCiCG8433-RB d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiEXT2_DROME
AccessioniPrimary (citable) accession number: Q9Y169
Secondary accession number(s): A4UZJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health