Reviewed,
UniProtKB/Swiss-Prot Q9Y169 (EXT2_DROME)
Last modified
January 19, 2010.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Exostosin-2 EC=2.4.1.224 EC=2.4.1.225 Alternative name(s): Protein sister of tout-velu | ||||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||||
| Taxonomic identifier | 7227 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 717 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Plays a central role in diffusion of morphogens hedgehog (hh), wingless (wg) and Decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of Hh, Dpp and wg morphogens. Ref.5 Ref.6 Ref.7 |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan. |
| Pathway | Protein modification; protein glycosylation. |
| Subunit structure | Forms a homo/hetero-oligomeric complex with ttv Probable. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. |
| Tissue specificity | In wing imaginal disk, it is ubiquitously expressed. Ref.5 |
| Developmental stage | Expressed both maternally and zygotically. Ref.7 |
| Sequence similarities | Belongs to the glycosyltransferase 47 family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Q9VMY9 | 1 | EBI-142791,EBI-151773 | ||
| ttv | Q9V730 | 1 | EBI-142791,EBI-166374 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 717 | 717 | Exostosin-2 | PRO_0000149665 | |||||
Regions | |||||||||
| Topological domain | 1 – 25 | 25 | Cytoplasmic Potential | ||||||
| Transmembrane | 26 – 46 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 47 – 717 | 671 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 91 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 574 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 636 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE013599 Genomic DNA. Translation: AAF58055.2. AF145598 mRNA. Translation: AAD38573.1. BT003544 mRNA. Translation: AAO39548.1. |
| RefSeq | NP_725536.1. NP_725537.2. |
| UniGene | Dm.20855 |
3D structure databases | |
| SMR | Q9Y169. Positions 453-700. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9Y169. 3 interactions. |
| STRING | Q9Y169. |
Protein family/group databases | |
| CAZy | GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64. |
Proteomic databases | |
| PRIDE | Q9Y169. |
Genome annotation databases | |
| Ensembl | FBtr0100512; FBpp0099952; FBgn0029175; Drosophila melanogaster. [Genome view] FBtr0100513; FBpp0099953; FBgn0029175; Drosophila melanogaster. [Genome view] |
| GeneID | 3772101. |
| KEGG | dme:Dmel_CG8433. |
Organism-specific databases | |
| CTD | 3772101. |
| FlyBase | FBgn0029175. Ext2. |
Phylogenomic databases | |
| eggNOG | inNOG07969. |
| InParanoid | Q9Y169. |
| OMA | QMALHPE. |
| OrthoDB | EOG94QT6V. |
| PhylomeDB | Q9Y169. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.224. 48. 2.4.1.225. 48. |
Gene expression databases | |
| GermOnline | CG8433. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view] |
| Pfam | PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 852446. |
Entry information
| Entry name | EXT2_DROME | ||||||||
| Accession | Primary (citable) accession number: Q9Y169 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


