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Q9Y0I1

- EAF3_DROME

UniProt

Q9Y0I1 - EAF3_DROME

Protein

NuA4 complex subunit EAF3 homolog

Gene

MRG15

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Part of the Tip60 chromatin-remodeling complex which is involved in DNA repair. Upon induction of DNA double-strand breaks, this complex acetylates phosphorylated H2AV in nucleosomes and exchanges it with unmodified H2AV.1 Publication

    GO - Molecular functioni

    1. methylated histone binding Source: FlyBase

    GO - Biological processi

    1. chromatin silencing Source: FlyBase
    2. DNA repair Source: UniProtKB-KW
    3. histone acetylation Source: UniProtKB
    4. histone exchange Source: UniProtKB
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NuA4 complex subunit EAF3 homolog
    Alternative name(s):
    Protein MRG15
    Gene namesi
    Name:MRG15
    ORF Names:CG6363
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0027378. MRG15.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. histone acetyltransferase complex Source: FlyBase
    2. NuA4 histone acetyltransferase complex Source: UniProtKB
    3. nucleus Source: FlyBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424NuA4 complex subunit EAF3 homologPRO_0000088771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei119 – 1191Phosphoserine1 Publication
    Modified residuei175 – 1751Phosphothreonine1 Publication
    Modified residuei183 – 1831Phosphothreonine1 Publication
    Modified residuei196 – 1961Phosphothreonine1 Publication
    Modified residuei197 – 1971Phosphothreonine1 Publication
    Modified residuei211 – 2111Phosphoserine1 Publication
    Modified residuei235 – 2351Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9Y0I1.
    PRIDEiQ9Y0I1.

    Expressioni

    Gene expression databases

    BgeeiQ9Y0I1.

    Interactioni

    Subunit structurei

    Component of the Tip60 chromatin-remodeling complex which contains the catalytic subunit Tip60 and the subunits Domino, Tra1, Brd8, E(Pc), DMAP1, Pontin, Reptin, Ing3, Act87E, BAP55, Mrg15, MrgBP, Gas41 and YL-1.1 Publication

    Protein-protein interaction databases

    BioGridi66914. 32 interactions.
    DIPiDIP-20132N.
    IntActiQ9Y0I1. 2 interactions.
    MINTiMINT-838509.

    Structurei

    Secondary structure

    1
    424
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi19 – 213
    Beta strandi27 – 315
    Beta strandi33 – 4412
    Beta strandi47 – 504
    Beta strandi52 – 576
    Helixi62 – 643
    Beta strandi66 – 694
    Helixi70 – 723
    Helixi78 – 9114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LRQNMR-A11-94[»]
    ProteinModelPortaliQ9Y0I1.
    SMRiQ9Y0I1. Positions 11-94, 256-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini252 – 424173MRGPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MRG domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG238705.
    GeneTreeiENSGT00530000063018.
    InParanoidiQ9Y0I1.
    KOiK11339.
    OrthoDBiEOG7ZPNK7.
    PhylomeDBiQ9Y0I1.

    Family and domain databases

    InterProiIPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR008676. MRG.
    IPR026541. MRG_dom.
    IPR025995. Tudor-knot.
    [Graphical view]
    PANTHERiPTHR10880. PTHR10880. 1 hit.
    PfamiPF05712. MRG. 1 hit.
    PF11717. Tudor-knot. 1 hit.
    [Graphical view]
    SMARTiSM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF54160. SSF54160. 2 hits.
    PROSITEiPS51640. MRG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Y0I1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEVKPAKVE NYSTGTDANT LFVDGERVLC FHGPLIYEAK VLKTKPDATP    50
    VEYYIHYAGW SKNWDEWVPE NRVLKYNDDN VKRRQELARQ CGERSKKDNK 100
    KGSAKAKKME QMRNESRAST PSKDSNTSQS TASSTPTTSA GPGSKSEAGS 150
    TGTTTTNSTA NSTTSRAHRK STQSTPSTAR PGTPSDKKED PAAAETTEEE 200
    GPVAPKKKRM SEQRPSLTGS DVAEKPLPPT TTPSTPTTEP APCVESEEAY 250
    AAKVEVKIKI PDELKHYLTD DWYAVVREHK LLELPAKVTV QQISEQYLAH 300
    KKSVKSTSAS KEVAINDVLD GIVEYFNVML GSQLLYKFER TQYADVMQKH 350
    PDTPLSELYG SFHLLRLFVR LGSMLSYSAL DQQSMQNLLT HVQDFLKFLV 400
    KNSSIFFSMS NFINVDPEYV RNAQ 424
    Length:424
    Mass (Da):47,194
    Last modified:November 1, 1999 - v1
    Checksum:iB0E1F615252D8EDD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF152245 mRNA. Translation: AAD38047.1.
    AE014297 Genomic DNA. Translation: AAF55161.1.
    AY051679 mRNA. Translation: AAK93103.1.
    RefSeqiNP_650442.1. NM_142185.2.
    UniGeneiDm.3453.

    Genome annotation databases

    EnsemblMetazoaiFBtr0083125; FBpp0082579; FBgn0027378.
    GeneIDi41850.
    KEGGidme:Dmel_CG6363.
    UCSCiCG6363-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF152245 mRNA. Translation: AAD38047.1 .
    AE014297 Genomic DNA. Translation: AAF55161.1 .
    AY051679 mRNA. Translation: AAK93103.1 .
    RefSeqi NP_650442.1. NM_142185.2.
    UniGenei Dm.3453.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LRQ NMR - A 11-94 [» ]
    ProteinModelPortali Q9Y0I1.
    SMRi Q9Y0I1. Positions 11-94, 256-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 66914. 32 interactions.
    DIPi DIP-20132N.
    IntActi Q9Y0I1. 2 interactions.
    MINTi MINT-838509.

    Proteomic databases

    PaxDbi Q9Y0I1.
    PRIDEi Q9Y0I1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0083125 ; FBpp0082579 ; FBgn0027378 .
    GeneIDi 41850.
    KEGGi dme:Dmel_CG6363.
    UCSCi CG6363-RA. d. melanogaster.

    Organism-specific databases

    CTDi 84045.
    FlyBasei FBgn0027378. MRG15.

    Phylogenomic databases

    eggNOGi NOG238705.
    GeneTreei ENSGT00530000063018.
    InParanoidi Q9Y0I1.
    KOi K11339.
    OrthoDBi EOG7ZPNK7.
    PhylomeDBi Q9Y0I1.

    Miscellaneous databases

    GenomeRNAii 41850.
    NextBioi 825893.
    PROi Q9Y0I1.

    Gene expression databases

    Bgeei Q9Y0I1.

    Family and domain databases

    InterProi IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR008676. MRG.
    IPR026541. MRG_dom.
    IPR025995. Tudor-knot.
    [Graphical view ]
    PANTHERi PTHR10880. PTHR10880. 1 hit.
    Pfami PF05712. MRG. 1 hit.
    PF11717. Tudor-knot. 1 hit.
    [Graphical view ]
    SMARTi SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54160. SSF54160. 2 hits.
    PROSITEi PS51640. MRG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conservation of the MORF4 related gene family: identification of a new chromo domain subfamily and novel protein motif."
      Bertram M.J., Pereira-Smith O.M.
      Gene 266:111-121(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    5. "Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions."
      Kusch T., Florens L., Macdonald W.H., Swanson S.K., Glaser R.L., Yates J.R. III, Abmayr S.M., Washburn M.P., Workman J.L.
      Science 306:2084-2087(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TIP60 COMPLEX, FUNCTION.
    6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119; THR-175; THR-183; THR-196; THR-197; SER-211 AND THR-235, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiEAF3_DROME
    AccessioniPrimary (citable) accession number: Q9Y0I1
    Secondary accession number(s): Q9VF99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3