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Protein

E3 ubiquitin-protein ligase Su(dx)

Gene

Su(dx)

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch/N signaling pathway, probably by promoting Notch ubiquitination, endocytosis and degradation. Involved in wing growth and leg joint formation.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei917Glycyl thioester intermediateCurated1

GO - Molecular functioni

  • Notch binding Source: UniProtKB
  • protein domain specific binding Source: FlyBase
  • ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  • imaginal disc-derived leg joint morphogenesis Source: UniProtKB
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: UniProtKB
  • lipid storage Source: FlyBase
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • Notch signaling pathway Source: FlyBase
  • protein ubiquitination Source: FlyBase
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • pupal development Source: UniProtKB
  • receptor internalization Source: UniProtKB
  • regulation of protein localization Source: FlyBase
  • wing disc dorsal/ventral pattern formation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Notch signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DME-1253288. Downregulation of ERBB4 signaling.
R-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
SignaLinkiQ9Y0H4.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Su(dx) (EC:2.3.2.26)
Alternative name(s):
HECT-type E3 ubiquitin transferase Su(dx)
Protein suppressor of deltex
Gene namesi
Name:Su(dx)
ORF Names:CG4244
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003557. Su(dx).

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: FlyBase
  • cytoplasm Source: FlyBase
  • nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203311 – 949E3 ubiquitin-protein ligase Su(dx)Add BLAST949

Proteomic databases

PaxDbiQ9Y0H4.
PRIDEiQ9Y0H4.

Expressioni

Tissue specificityi

Expressed in pupal wings, at the boundary between vein and intervein territories.1 Publication

Gene expression databases

BgeeiFBgn0003557.
ExpressionAtlasiQ9Y0H4. baseline.
GenevisibleiQ9Y0H4. DM.

Interactioni

Subunit structurei

Interacts with N.1 Publication

GO - Molecular functioni

  • Notch binding Source: UniProtKB
  • protein domain specific binding Source: FlyBase

Protein-protein interaction databases

BioGridi59611. 16 interactors.
IntActiQ9Y0H4. 6 interactors.
MINTiMINT-1015939.
STRINGi7227.FBpp0077495.

Structurei

Secondary structure

1949
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi477 – 482Combined sources6
Beta strandi485 – 488Combined sources4
Turni489 – 491Combined sources3
Beta strandi492 – 497Combined sources6
Turni498 – 501Combined sources4
Beta strandi502 – 507Combined sources6
Turni511 – 513Combined sources3
Helixi516 – 519Combined sources4
Beta strandi528 – 532Combined sources5
Turni533 – 535Combined sources3
Beta strandi536 – 541Combined sources6
Turni542 – 545Combined sources4
Beta strandi546 – 552Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TK7NMR-A474-554[»]
2JMFNMR-A515-557[»]
ProteinModelPortaliQ9Y0H4.
SMRiQ9Y0H4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y0H4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 129C2PROSITE-ProRule annotationAdd BLAST94
Domaini363 – 396WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini395 – 428WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini477 – 510WW 3PROSITE-ProRule annotationAdd BLAST34
Domaini521 – 554WW 4PROSITE-ProRule annotationAdd BLAST34
Domaini615 – 949HECTPROSITE-ProRule annotationAdd BLAST335

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi261 – 271Poly-GlnAdd BLAST11
Compositional biasi456 – 460Poly-Gln5

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
Contains 4 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0940. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
InParanoidiQ9Y0H4.
KOiK05633.
OMAiWEIRKDG.
OrthoDBiEOG091G0SS8.
PhylomeDBiQ9Y0H4.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT_dom.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF00397. WW. 4 hits.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 4 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 4 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 3 hits.
PS50020. WW_DOMAIN_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y0H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGNGLPAG AASGGMEAGQ TVNGAGSASP TPTSSSGAGA SGSANQGYHQ
60 70 80 90 100
LSVTIEEASL RNNGFLKPNP YVELLIDSKS KRKTDLVKNS YLPKWNEEFT
110 120 130 140 150
VLITPNSTLH FKVLDHSSFR KDAMLGERII NLAHILQHYN GRCEFLELTI
160 170 180 190 200
DLFVTSKSDN RQTKSGELVA ILNGLKLDMS KLQIQPVAGQ QNGNPPVQAV
210 220 230 240 250
NPSVVSDAAA GRSCMIYGGV RARMRLRSSS GNSNGGETRS PLPNGGGDHR
260 270 280 290 300
RSTQAPPVWE QQQQQSQNQQ QPLRMVNGSG AAVPQTAPYP QQPPAPALAR
310 320 330 340 350
PLTQVYGALP ENTQPAAVYL PAGGGAAVGP PGVAGPPIEQ PGVGLPVSQS
360 370 380 390 400
TDPQLQTQPA DDEPLPAGWE IRLDQYGRRY YVDHNTRSTY WEKPTPLPPG
410 420 430 440 450
WEIRKDGRGR VYYVDHNTRK TTWQRPNSER LMHFQHWQGQ RAHVVSQGNQ
460 470 480 490 500
RYLYSQQQQQ PTAVTAQVTQ DDEDALGPLP DGWEKKIQSD NRVYFVNHKN
510 520 530 540 550
RTTQWEDPRT QGQEVSLINE GPLPPGWEIR YTAAGERFFV DHNTRRTTFE
560 570 580 590 600
DPRPGAPKGA KGVYGVPRAY ERSFRWKLSQ FRYLCQSNAL PSHIKITVTR
610 620 630 640 650
QTLFEDSYHQ IMRLPAYELR RRLYIIFRGE EGLDYGGVSR EWFFLLSHEV
660 670 680 690 700
LNPMYCLFEY ANKNNYSLQI NPASYVNPDH LQYFKFIGRF IAMALYHGRF
710 720 730 740 750
IYSGFTMPFY KRMLNKKLTI KDIETIDPEF YNSLIWVKDN NIDECGLELW
760 770 780 790 800
FSVDFEVLGQ IIHHELKENG EKERVTEENK EEYITLMTEW RMTRGIEQQT
810 820 830 840 850
KTFLEGFNEV VPLEWLKYFD ERELELILCG MQDVDVEDWQ RNTIYRHYNR
860 870 880 890 900
NSKQVVWFWQ FVRETDNEKR ARLLQFVTGT CRVPVGGFAE LMGSNGPQRF
910 920 930 940
CIEKVGKETW LPRSHTCFNR LDLPPYKSYD QLVEKLTFAI EETEGFCQE
Length:949
Mass (Da):107,966
Last modified:November 1, 1999 - v1
Checksum:i74B17A8B05AC6E6B
GO

Sequence cautioni

The sequence AAL39551 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152865 mRNA. Translation: AAD38975.1.
AE014134 Genomic DNA. Translation: AAF51311.1.
AE014134 Genomic DNA. Translation: AAN10440.1.
BT021390 mRNA. Translation: AAX33538.1.
AY069406 mRNA. Translation: AAL39551.1. Different initiation.
RefSeqiNP_001259898.1. NM_001272969.1.
NP_001259899.1. NM_001272970.1.
NP_476753.1. NM_057405.3.
NP_722753.1. NM_164448.2.
NP_722754.1. NM_164449.2.
UniGeneiDm.2146.

Genome annotation databases

EnsemblMetazoaiFBtr0077822; FBpp0077495; FBgn0003557.
FBtr0077823; FBpp0077496; FBgn0003557.
FBtr0077824; FBpp0077497; FBgn0003557.
FBtr0334778; FBpp0306832; FBgn0003557.
FBtr0339529; FBpp0308612; FBgn0003557.
GeneIDi33379.
KEGGidme:Dmel_CG4244.
UCSCiCG4244-RC. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152865 mRNA. Translation: AAD38975.1.
AE014134 Genomic DNA. Translation: AAF51311.1.
AE014134 Genomic DNA. Translation: AAN10440.1.
BT021390 mRNA. Translation: AAX33538.1.
AY069406 mRNA. Translation: AAL39551.1. Different initiation.
RefSeqiNP_001259898.1. NM_001272969.1.
NP_001259899.1. NM_001272970.1.
NP_476753.1. NM_057405.3.
NP_722753.1. NM_164448.2.
NP_722754.1. NM_164449.2.
UniGeneiDm.2146.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TK7NMR-A474-554[»]
2JMFNMR-A515-557[»]
ProteinModelPortaliQ9Y0H4.
SMRiQ9Y0H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59611. 16 interactors.
IntActiQ9Y0H4. 6 interactors.
MINTiMINT-1015939.
STRINGi7227.FBpp0077495.

Proteomic databases

PaxDbiQ9Y0H4.
PRIDEiQ9Y0H4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077822; FBpp0077495; FBgn0003557.
FBtr0077823; FBpp0077496; FBgn0003557.
FBtr0077824; FBpp0077497; FBgn0003557.
FBtr0334778; FBpp0306832; FBgn0003557.
FBtr0339529; FBpp0308612; FBgn0003557.
GeneIDi33379.
KEGGidme:Dmel_CG4244.
UCSCiCG4244-RC. d. melanogaster.

Organism-specific databases

CTDi33379.
FlyBaseiFBgn0003557. Su(dx).

Phylogenomic databases

eggNOGiKOG0940. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
InParanoidiQ9Y0H4.
KOiK05633.
OMAiWEIRKDG.
OrthoDBiEOG091G0SS8.
PhylomeDBiQ9Y0H4.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DME-1253288. Downregulation of ERBB4 signaling.
R-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
SignaLinkiQ9Y0H4.

Miscellaneous databases

EvolutionaryTraceiQ9Y0H4.
GenomeRNAii33379.
PROiQ9Y0H4.

Gene expression databases

BgeeiFBgn0003557.
ExpressionAtlasiQ9Y0H4. baseline.
GenevisibleiQ9Y0H4. DM.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT_dom.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF00397. WW. 4 hits.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 4 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 4 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 3 hits.
PS50020. WW_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUDX_DROME
AccessioniPrimary (citable) accession number: Q9Y0H4
Secondary accession number(s): A4UZZ3, Q0E8U5, Q8T0C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.