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Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

ppp4c

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for development, chemotaxis and the expression of numerous genes.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Manganese 1By similarity
Metal bindingi54 – 541Manganese 1By similarity
Metal bindingi80 – 801Manganese 1By similarity
Metal bindingi80 – 801Manganese 2By similarity
Metal bindingi112 – 1121Manganese 2By similarity
Active sitei113 – 1131Proton donorBy similarity
Metal bindingi162 – 1621Manganese 2By similarity
Metal bindingi236 – 2361Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: dictyBase

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. regulation of chemotaxis Source: dictyBase
  3. regulation of sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Gene namesi
Name:ppp4c
Synonyms:pppC
ORF Names:DDB_G0272116
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272116. ppp4c.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Translocated to the nucleus in aggregation-competent cells, probably by smkA.

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
  2. nucleus Source: UniProtKB-SubCell
  3. protein phosphatase 4 complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000327847Add
BLAST

Proteomic databases

PRIDEiQ9Y0B7.

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Probably part of a PP4 complex containing ppp4c and ppp4r2. Interacts with smkA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
smkAQ54I182EBI-2015876,EBI-2015890

Protein-protein interaction databases

IntActiQ9Y0B7. 1 interaction.
STRINGi44689.DDB_0185222.

Structurei

3D structure databases

ProteinModelPortaliQ9Y0B7.
SMRiQ9Y0B7. Positions 2-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ9Y0B7.
KOiK15423.
OMAiQFNYKND.
PhylomeDBiQ9Y0B7.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y0B7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSDLDRQIE QLKRCEIIKE SEVRALCSKA REILLEEGNV QRVDSPVTIC
60 70 80 90 100
GDIHGQFYDL KELFKVGGDC PQTNYLFMGD FVDRGFYSVE TFLLLLALKV
110 120 130 140 150
RYPDRITLIR GNHESRQITQ VYGFYEECVR KYGSVTVWKY CTEIFDYLSL
160 170 180 190 200
SALVDGKIFC VHGGLSPSIN TLDQIRAIDR KQEVPHEGPM CDLMWSDPED
210 220 230 240 250
IPGWNGSPRG AGFLFGEDVV QKFNHDNNLE FICRAHQLVM EGFKYMFNET
260 270 280 290 300
LVTVWSAPNY CYRCGNVAAI LQLDENLKKN FAIFEAAPQE SRGAPAKKPA

PEYFL
Length:305
Mass (Da):34,862
Last modified:November 1, 1999 - v1
Checksum:iC0903104ADA93D41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161253 mRNA. Translation: AAD43137.1.
AAFI02000008 Genomic DNA. Translation: EAL71210.1.
RefSeqiXP_645186.1. XM_640094.1.

Genome annotation databases

EnsemblProtistsiDDB0185222; DDB0185222; DDB_G0272116.
GeneIDi8618358.
KEGGiddi:DDB_G0272116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161253 mRNA. Translation: AAD43137.1.
AAFI02000008 Genomic DNA. Translation: EAL71210.1.
RefSeqiXP_645186.1. XM_640094.1.

3D structure databases

ProteinModelPortaliQ9Y0B7.
SMRiQ9Y0B7. Positions 2-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Y0B7. 1 interaction.
STRINGi44689.DDB_0185222.

Proteomic databases

PRIDEiQ9Y0B7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0185222; DDB0185222; DDB_G0272116.
GeneIDi8618358.
KEGGiddi:DDB_G0272116.

Organism-specific databases

dictyBaseiDDB_G0272116. ppp4c.

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ9Y0B7.
KOiK15423.
OMAiQFNYKND.
PhylomeDBiQ9Y0B7.

Miscellaneous databases

PROiQ9Y0B7.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis."
    Mendoza M.C., Booth E.O., Shaulsky G., Firtel R.A.
    Mol. Cell. Biol. 27:3817-3827(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMKA AND PPP4R2.

Entry informationi

Entry nameiPP4C_DICDI
AccessioniPrimary (citable) accession number: Q9Y0B7
Secondary accession number(s): Q559Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1999
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.