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Q9Y0B7

- PP4C_DICDI

UniProt

Q9Y0B7 - PP4C_DICDI

Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

ppp4c

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Required for development, chemotaxis and the expression of numerous genes.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi52 – 521Manganese 1By similarity
    Metal bindingi54 – 541Manganese 1By similarity
    Metal bindingi80 – 801Manganese 1By similarity
    Metal bindingi80 – 801Manganese 2By similarity
    Metal bindingi112 – 1121Manganese 2By similarity
    Active sitei113 – 1131Proton donorBy similarity
    Metal bindingi162 – 1621Manganese 2By similarity
    Metal bindingi236 – 2361Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: dictyBase

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. regulation of chemotaxis Source: dictyBase
    3. regulation of sorocarp development Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
    Short name:
    PP4C
    Gene namesi
    Name:ppp4c
    Synonyms:pppC
    ORF Names:DDB_G0272116
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0272116. ppp4c.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Translocated to the nucleus in aggregation-competent cells, probably by smkA.

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. nucleus Source: UniProtKB-SubCell
    3. protein phosphatase 4 complex Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000327847Add
    BLAST

    Proteomic databases

    PRIDEiQ9Y0B7.

    Interactioni

    Subunit structurei

    Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Probably part of a PP4 complex containing ppp4c and ppp4r2. Interacts with smkA.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    smkAQ54I182EBI-2015876,EBI-2015890

    Protein-protein interaction databases

    IntActiQ9Y0B7. 1 interaction.
    STRINGi44689.DDB_0185222.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y0B7.
    SMRiQ9Y0B7. Positions 2-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    KOiK15423.
    OMAiGSNAGPN.
    PhylomeDBiQ9Y0B7.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Y0B7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSDLDRQIE QLKRCEIIKE SEVRALCSKA REILLEEGNV QRVDSPVTIC    50
    GDIHGQFYDL KELFKVGGDC PQTNYLFMGD FVDRGFYSVE TFLLLLALKV 100
    RYPDRITLIR GNHESRQITQ VYGFYEECVR KYGSVTVWKY CTEIFDYLSL 150
    SALVDGKIFC VHGGLSPSIN TLDQIRAIDR KQEVPHEGPM CDLMWSDPED 200
    IPGWNGSPRG AGFLFGEDVV QKFNHDNNLE FICRAHQLVM EGFKYMFNET 250
    LVTVWSAPNY CYRCGNVAAI LQLDENLKKN FAIFEAAPQE SRGAPAKKPA 300
    PEYFL 305
    Length:305
    Mass (Da):34,862
    Last modified:November 1, 1999 - v1
    Checksum:iC0903104ADA93D41
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF161253 mRNA. Translation: AAD43137.1.
    AAFI02000008 Genomic DNA. Translation: EAL71210.1.
    RefSeqiXP_645186.1. XM_640094.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185222; DDB0185222; DDB_G0272116.
    GeneIDi8618358.
    KEGGiddi:DDB_G0272116.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF161253 mRNA. Translation: AAD43137.1 .
    AAFI02000008 Genomic DNA. Translation: EAL71210.1 .
    RefSeqi XP_645186.1. XM_640094.1.

    3D structure databases

    ProteinModelPortali Q9Y0B7.
    SMRi Q9Y0B7. Positions 2-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Y0B7. 1 interaction.
    STRINGi 44689.DDB_0185222.

    Proteomic databases

    PRIDEi Q9Y0B7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185222 ; DDB0185222 ; DDB_G0272116 .
    GeneIDi 8618358.
    KEGGi ddi:DDB_G0272116.

    Organism-specific databases

    dictyBasei DDB_G0272116. ppp4c.

    Phylogenomic databases

    eggNOGi COG0639.
    KOi K15423.
    OMAi GSNAGPN.
    PhylomeDBi Q9Y0B7.

    Miscellaneous databases

    PROi Q9Y0B7.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis."
      Mendoza M.C., Booth E.O., Shaulsky G., Firtel R.A.
      Mol. Cell. Biol. 27:3817-3827(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMKA AND PPP4R2.

    Entry informationi

    Entry nameiPP4C_DICDI
    AccessioniPrimary (citable) accession number: Q9Y0B7
    Secondary accession number(s): Q559Z8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3