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Q9Y0B7 (PP4C_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit

Short name=PP4C
EC=3.1.3.16
Gene names
Name:ppp4c
Synonyms:pppC
ORF Names:DDB_G0272116
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for development, chemotaxis and the expression of numerous genes. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Probably part of a PP4 complex containing ppp4c and ppp4r2. Interacts with smkA. Ref.4

Subcellular location

Cytoplasm. Nucleus. Note: Translocated to the nucleus in aggregation-competent cells, probably by smkA. Ref.4

Sequence similarities

Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

smkAQ54I182EBI-2015876,EBI-2015890

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Serine/threonine-protein phosphatase 4 catalytic subunit
PRO_0000327847

Sites

Active site1131Proton donor By similarity
Metal binding521Manganese 1 By similarity
Metal binding541Manganese 1 By similarity
Metal binding801Manganese 1 By similarity
Metal binding801Manganese 2 By similarity
Metal binding1121Manganese 2 By similarity
Metal binding1621Manganese 2 By similarity
Metal binding2361Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9Y0B7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: C0903104ADA93D41

FASTA30534,862
        10         20         30         40         50         60 
MSSDLDRQIE QLKRCEIIKE SEVRALCSKA REILLEEGNV QRVDSPVTIC GDIHGQFYDL 

        70         80         90        100        110        120 
KELFKVGGDC PQTNYLFMGD FVDRGFYSVE TFLLLLALKV RYPDRITLIR GNHESRQITQ 

       130        140        150        160        170        180 
VYGFYEECVR KYGSVTVWKY CTEIFDYLSL SALVDGKIFC VHGGLSPSIN TLDQIRAIDR 

       190        200        210        220        230        240 
KQEVPHEGPM CDLMWSDPED IPGWNGSPRG AGFLFGEDVV QKFNHDNNLE FICRAHQLVM 

       250        260        270        280        290        300 
EGFKYMFNET LVTVWSAPNY CYRCGNVAAI LQLDENLKKN FAIFEAAPQE SRGAPAKKPA 


PEYFL 

« Hide

References

« Hide 'large scale' references
[1]"Searching for the role of protein phosphatases in eukaryotic microorganisms."
da-Silva A.M., Zapella P.D.A., Andrioli L.P.M., Campanha R.B., Fiorini L.C., Etchebehere L.C., da-Costa-Maia J.C., Terenzi H.F.
Braz. J. Med. Biol. Res. 32:835-839(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX4.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis."
Mendoza M.C., Booth E.O., Shaulsky G., Firtel R.A.
Mol. Cell. Biol. 27:3817-3827(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMKA AND PPP4R2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF161253 mRNA. Translation: AAD43137.1.
AAFI02000008 Genomic DNA. Translation: EAL71210.1.
RefSeqXP_645186.1. XM_640094.1.

3D structure databases

ProteinModelPortalQ9Y0B7.
SMRQ9Y0B7. Positions 2-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9Y0B7. 1 interaction.
STRING44689.DDB_0185222.

Proteomic databases

PRIDEQ9Y0B7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185222; DDB0185222; DDB_G0272116.
GeneID8618358.
KEGGddi:DDB_G0272116.

Organism-specific databases

dictyBaseDDB_G0272116. ppp4c.

Phylogenomic databases

eggNOGCOG0639.
OMAGSNAGPN.
PhylomeDBQ9Y0B7.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9Y0B7.

Entry information

Entry namePP4C_DICDI
AccessionPrimary (citable) accession number: Q9Y0B7
Secondary accession number(s): Q559Z8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase