Q9Y0B7 (PP4C_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit
Short name=PP4C
EC=3.1.3.16

Gene names

Name:	ppp4c
Synonyms:	pppC
ORF Names:	DDB_G0272116

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for development, chemotaxis and the expression of numerous genes. Ref.4
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Probably part of a PP4 complex containing ppp4c and ppp4r2. Interacts with smkA. Ref.4
Subcellular location	Cytoplasm. Nucleus. Note: Translocated to the nucleus in aggregation-competent cells, probably by smkA. Ref.4
Sequence similarities	Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of chemotaxis Inferred from genetic interaction Ref.4. Source: dictyBase regulation of sorocarp development Inferred from mutant phenotype Ref.4. Source: dictyBase
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: dictyBase nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell protein phosphatase 4 complex Inferred from direct assay Ref.4. Source: dictyBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay Ref.4. Source: dictyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
smkA	Q54I18	2	EBI-2015876,EBI-2015890

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Serine/threonine-protein phosphatase 4 catalytic subunit		PRO_0000327847
Sites
	Active site	113	1	Proton donor By similarity
	Metal binding	52	1	Iron By similarity
	Metal binding	54	1	Iron By similarity
	Metal binding	80	1	Iron By similarity
	Metal binding	80	1	Manganese By similarity
	Metal binding	112	1	Manganese By similarity
	Metal binding	162	1	Manganese By similarity
	Metal binding	236	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y0B7 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: C0903104ADA93D41	FASTA	305	34,862
	10 20 30 40 50 60 MSSDLDRQIE QLKRCEIIKE SEVRALCSKA REILLEEGNV QRVDSPVTIC GDIHGQFYDL 70 80 90 100 110 120 KELFKVGGDC PQTNYLFMGD FVDRGFYSVE TFLLLLALKV RYPDRITLIR GNHESRQITQ 130 140 150 160 170 180 VYGFYEECVR KYGSVTVWKY CTEIFDYLSL SALVDGKIFC VHGGLSPSIN TLDQIRAIDR 190 200 210 220 230 240 KQEVPHEGPM CDLMWSDPED IPGWNGSPRG AGFLFGEDVV QKFNHDNNLE FICRAHQLVM 250 260 270 280 290 300 EGFKYMFNET LVTVWSAPNY CYRCGNVAAI LQLDENLKKN FAIFEAAPQE SRGAPAKKPA PEYFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Searching for the role of protein phosphatases in eukaryotic microorganisms." da-Silva A.M., Zapella P.D.A., Andrioli L.P.M., Campanha R.B., Fiorini L.C., Etchebehere L.C., da-Costa-Maia J.C., Terenzi H.F. Braz. J. Med. Biol. Res. 32:835-839(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX4.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis." Mendoza M.C., Booth E.O., Shaulsky G., Firtel R.A. Mol. Cell. Biol. 27:3817-3827(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMKA AND PPP4R2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF161253 mRNA. Translation: AAD43137.1. AAFI02000008 Genomic DNA. Translation: EAL71210.1.
RefSeq	XP_645186.1. XM_640094.1.
3D structure databases
HSSP	HSSP built from PDB template 1FJM based on UniProtKB P62139.
ProteinModelPortal	Q9Y0B7.
SMR	Q9Y0B7. Positions 2-305.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y0B7. 1 interaction.
STRING	44689.DDB_0185222.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185222; DDB0185222; DDB_G0272116.
GeneID	8618358.
KEGG	ddi:DDB_G0272116.
Organism-specific databases
dictyBase	DDB_G0272116. ppp4c.
Phylogenomic databases
eggNOG	COG0639.
KO	K15423.
OMA	GFKWHFN.
ProtClustDB	CLSZ2729244.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP4C_DICDI

Accession

Primary (citable) accession number: Q9Y0B7
Secondary accession number(s): Q559Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)