Reviewed, UniProtKB/Swiss-Prot Q9Y041 (HGD_CAEEL)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisic acid oxidase
    Homogentisate oxygenase
    Homogentisicase

Gene names

Name:	hgo-1
ORF Names:	W06D4.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate.
Cofactor	Iron.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC identical protein binding Inferred from physical interaction. Source: IntAct iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-320396,EBI-320396

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Homogentisate 1,2-dioxygenase		PRO_0000220243
Sites
	Metal binding	336	1	Iron By similarity
	Metal binding	342	1	Iron By similarity
	Metal binding	372	1	Iron By similarity
Experimental info
	Sequence conflict	156	1	D → N in AAD00776. Ref.2
	Sequence conflict	176	1	L → P in AAD00776. Ref.2
	Sequence conflict	193	1	V → G in AAD00776. Ref.2
	Sequence conflict	242	1	V → G in AAD00776. Ref.2
	Sequence conflict	265	1	D → E in AAD00776. Ref.2
	Sequence conflict	433	1	F → Y in AAD00776. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Y041-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: C14E7077C7CF9703	FASTA	437	49,239
	10 20 30 40 50 60 MSEFDELKYL TGFGNEHATS DPRVPDALPV GQNSPQKCSH GLYAEQLSGT AFTAPRSQNQ 70 80 90 100 110 120 RSWLYRIRPS VIHRPFEAMK ENDQHWTNNF SSIPPNPNQY RWNPFPLPTK EGVTFVDNLY 130 140 150 160 170 180 TVCGGGDVIS RTGLAIHQFS CNASMEHTAM YNSDGDFLIV PQQGALEITT EFGRLLVNPQ 190 200 210 220 230 240 EIAVIPQGIR FSVAVRGPSR GYILEVYGTH FQLPDLGPIG ANGLANPRDF EAPVAWFEDL 250 260 270 280 290 300 DVEFTIINKY QGSWFQAKQG HSPFDVVGWH GNYVPYKYDL KKFMVINTVS FDHCDPSIFT 310 320 330 340 350 360 VLTAPSVKHG TAIADFVIFP PRWGCADNTF RPPYYHRNCM SEYMGLITGC YEAKEGGFKP 370 380 390 400 410 420 GGGSLHSMMT PHGPDFNCFE MASNADLKPQ RVAEGTMSFM FESSLNMAIT NWAVYQNVDK 430 DYYKDWQPLK KHFTMPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the homogentisate 1,2-dioxygenase gene in A. thaliana and C. elegans." Schmidt S.R., Werner E., Mueller C.R., Kress W. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bristol N2.
[2]	"Sequence homology of HGO genes in eukaryotic organisms." Schmidt S.R., Werner E., Mueller C.R., Kress W. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2. Tissue: Embryo.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF136150 Genomic DNA. Translation: AAF61419.1. U95181 mRNA. Translation: AAD00776.1. Z93778, AL034364 Genomic DNA. Translation: CAB07848.4. AL034364, Z93778 Genomic DNA. Translation: CAA22255.4.
PIR	T19626. T37469.
RefSeq	NP_492433.1.
UniGene	Cel.19613
3D structure databases
SMR	Q9Y041. Positions 6-434.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y041. 2 interactions.
STRING	Q9Y041.
Genome annotation databases
Ensembl	W06D4.1; W06D4.1; W06D4.1; Caenorhabditis elegans. [Genome view]
GeneID	172726.
KEGG	cel:W06D4.1.
NMPDR	fig|6239.3.peg.2394.
UCSC	W06D4.1. c. elegans.
Organism-specific databases
CTD	172726.
WormBase	WBGene00001843. hgo-1.
WormPep	W06D4.1. CE29602. [WorfDB]
Phylogenomic databases
eggNOG	meNOG05372.
HOGENOM	HBG293508.
InParanoid	Q9Y041.
OMA	RNCMSEF.
PhylomeDB	Q9Y041.
Enzyme and pathway databases
BRENDA	1.13.11.5. 672.
Gene expression databases
ArrayExpress	Q9Y041.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	876753.

Entry information

Entry name

HGD_CAEEL

Accession

Primary (citable) accession number: Q9Y041
Secondary accession number(s): O62087, Q9NJP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 27, 2001
Last modified:	February 9, 2010
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project