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Q9XZI6

- PICAL_CAEEL

UniProt

Q9XZI6 - PICAL_CAEEL

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Protein

Phosphatidylinositol-binding clathrin assembly protein unc-11

Gene

unc-11

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Required for the efficient targeting of the synaptic vesicle protein synaptobrevin.1 Publication

GO - Molecular functioni

  1. 1-phosphatidylinositol binding Source: UniProtKB
  2. clathrin binding Source: WormBase

GO - Biological processi

  1. clathrin coat assembly Source: InterPro
  2. locomotion Source: WormBase
  3. positive regulation of necrotic cell death Source: WormBase
  4. post-embryonic development Source: WormBase
  5. receptor-mediated endocytosis Source: UniProtKB
  6. synaptic vesicle endocytosis Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-binding clathrin assembly protein unc-11
Alternative name(s):
AP180-like adaptor protein
Uncoordinated protein 11
Gene namesi
Name:unc-11
ORF Names:C32E8.10
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiC32E8.10a; CE23562; WBGene00006751; unc-11.
C32E8.10b; CE27812; WBGene00006751; unc-11.
C32E8.10c; CE24822; WBGene00006751; unc-11.
C32E8.10d; CE23564; WBGene00006751; unc-11.
C32E8.10e; CE23565; WBGene00006751; unc-11.
C32E8.10f; CE23566; WBGene00006751; unc-11.
C32E8.10h; CE27813; WBGene00006751; unc-11.

Subcellular locationi

Membraneclathrin-coated pit 1 Publication. Golgi apparatus 1 Publication. Cytoplasmic vesicleclathrin-coated vesicle 1 Publication
Note: Colocalized with clathrin in the Golgi area.

GO - Cellular componenti

  1. clathrin coat Source: InterPro
  2. coated pit Source: UniProtKB
  3. cytoplasmic vesicle Source: UniProtKB-KW
  4. Golgi apparatus Source: UniProtKB-KW
  5. presynaptic membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586Phosphatidylinositol-binding clathrin assembly protein unc-11PRO_0000187065Add
BLAST

Proteomic databases

PaxDbiQ9XZI6.
PRIDEiQ9XZI6.

Expressioni

Tissue specificityi

High levels in the nervous system and at lower levels in other tissues. In neurons, enriched at presynaptic terminals and is also present in cell bodies.1 Publication

Interactioni

Subunit structurei

Binds clathrin and phosphatidylinositol 4,5-bisphosphate.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
eya-1O176704EBI-311866,EBI-311862
itsn-1Q9U2T93EBI-311866,EBI-2414252
magu-1Q95XW53EBI-311866,EBI-2317194

Protein-protein interaction databases

BioGridi37426. 3 interactions.
DIPiDIP-25315N.
IntActiQ9XZI6. 7 interactions.
MINTiMINT-1053765.

Structurei

3D structure databases

ProteinModelPortaliQ9XZI6.
SMRiQ9XZI6. Positions 37-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 161133ENTHCuratedPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG267212.
GeneTreeiENSGT00390000008805.
InParanoidiQ9XZI6.
OMAiITIHNIM.
OrthoDBiEOG76QFM8.
PhylomeDBiQ9XZI6.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
IPR001026. Epsin_dom_N.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (9)i

Sequence statusi: Complete.

This entry describes 9 isoformsi produced by alternative splicing. Align

Isoform A1 Publication (identifier: Q9XZI6) [UniParc]FASTAAdd to Basket

Also known as: aCurated

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTIEKALHQ PMPFTTGGQT ISDRLTAAKH SLAGSQLGKT ICKATTEEVM
60 70 80 90 100
APKKKHLDYL LHCTNEPNVS IPSMANLLIE RTQNPNWTVV YKALITIHNI
110 120 130 140 150
MCYGNERFSQ YLASCNTTFN LTAFVDKVGG AGGYDMSTHV RRYAKYIGEK
160 170 180 190 200
INTYRMCAFD FCKVKRGRED GLLRTMHTDK LLKTIPILQN QIDALLEFSV
210 220 230 240 250
TTSELNNGVI NCSFILLFRD LIRLFACYND GIINVLEKYF DMNKKQCRDA
260 270 280 290 300
LDTYKSFLTR LDKVAEFLRV AESVGIDRGE IPDLTRAPAS LLEALEAHLI
310 320 330 340 350
HLEGGKAPPP TQQHVAPHQF TTGFAFSQQP QPALGDAERQ RYIELEQERL
360 370 380 390 400
RQFEDQKKSI NSANPFANDV ASAAPAPATS AAQPDLLDMF QSSAAPAPQT
410 420 430 440 450
ADVTNPFGNF AAPSAFPTNV PPPAAHSAPF GVQPAPQHSA APFYANLHQA
460 470 480 490 500
PPMQSQAPNG HQAAPFGYPN AHPDDLARMT AQMSLNQQGA PAGWNTTTSA
510 520 530 540 550
VSNNPFGATS APQPMYTAPM GMYQQPFGAQ PMWNPAMAAY GQQYGYGQPV
560 570 580
PPQQQHQIQL VHAAMAAKNA AQAQQAQAAS ADPFGL
Length:586
Mass (Da):64,064
Last modified:November 1, 1999 - v1
Checksum:i6E337A81D302DD81
GO
Isoform B1 Publication (identifier: Q9XZI6-2) [UniParc]FASTAAdd to Basket

Also known as: hCurated

The sequence of this isoform differs from the canonical sequence as follows:
     419-458: Missing.
     489-489: G → GYR

Show »
Length:548
Mass (Da):60,273
Checksum:i8641691EF297D717
GO
Isoform C1 Publication (identifier: Q9XZI6-3) [UniParc]FASTAAdd to Basket

Also known as: bCurated

The sequence of this isoform differs from the canonical sequence as follows:
     419-458: Missing.

Show »
Length:546
Mass (Da):59,954
Checksum:i69D424C56BAF517A
GO
Isoform c (identifier: Q9XZI6-7) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-459: N → NEPENPFITAPAAPQMHNAPPVPPPPASQGAPAPINPFADPSATAASSAQPFGDPDDFKFEQKNVKIKILIP

Note: No experimental confirmation available.

Show »
Length:657
Mass (Da):71,443
Checksum:i891BA9FB3525AAD2
GO
Isoform D1 Publication (identifier: Q9XZI6-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-449: Missing.
     489-489: G → GYR

Show »
Length:535
Mass (Da):58,978
Checksum:i7EB8FC25A2896AA5
GO
Isoform d (identifier: Q9XZI6-8) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-408: APQTADVTNPFG → LLQCNRKLQMVS
     409-460: Missing.

Note: No experimental confirmation available.

Show »
Length:534
Mass (Da):58,994
Checksum:i4FDEE1FAEDBA4847
GO
Isoform E1 Publication (identifier: Q9XZI6-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-512: Missing.

Show »
Length:456
Mass (Da):50,727
Checksum:i44EDE64136212A9E
GO
Isoform e (identifier: Q9XZI6-9) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-467: QPDLLDMFQS...PNGHQAAPFG → HQCIRLQWEC...LVVYFSLSLS
     468-586: Missing.

Note: No experimental confirmation available.

Show »
Length:467
Mass (Da):53,131
Checksum:i42042675FC806DA9
GO
Isoform fCurated (identifier: Q9XZI6-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-388: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:198
Mass (Da):20,632
Checksum:i2F38F15C93F0F0C1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 388388Missing in isoform f. CuratedVSP_050679Add
BLAST
Alternative sequencei383 – 512130Missing in isoform E. 1 PublicationVSP_050680Add
BLAST
Alternative sequencei383 – 46785QPDLL…AAPFG → HQCIRLQWECINNHLVLSQC GILRWQHTVNNMDTDNLFHH NSNTKFNWFMQRWLQRTLHK LNRPRPPQPIHLDYSLVVYF SLSLS in isoform e. 1 PublicationVSP_020098Add
BLAST
Alternative sequencei397 – 44953Missing in isoform D. 1 PublicationVSP_050681Add
BLAST
Alternative sequencei397 – 40812APQTA…TNPFG → LLQCNRKLQMVS in isoform d. 1 PublicationVSP_020099Add
BLAST
Alternative sequencei409 – 46052Missing in isoform d. 1 PublicationVSP_020100Add
BLAST
Alternative sequencei419 – 45840Missing in isoform C and isoform B. 1 PublicationVSP_050682Add
BLAST
Alternative sequencei459 – 4591N → NEPENPFITAPAAPQMHNAP PVPPPPASQGAPAPINPFAD PSATAASSAQPFGDPDDFKF EQKNVKIKILIP in isoform c. 1 PublicationVSP_020101
Alternative sequencei468 – 586119Missing in isoform e. 1 PublicationVSP_020102Add
BLAST
Alternative sequencei489 – 4891G → GYR in isoform B and isoform D. 1 PublicationVSP_050683

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF144257 mRNA. Translation: AAD37365.1.
AF144258 mRNA. Translation: AAD37366.1.
AF144259 mRNA. Translation: AAD37367.1.
AF144260 mRNA. Translation: AAD37368.1.
AF144261 mRNA. Translation: AAD37369.1.
AF144262 mRNA. Translation: AAD37370.1.
FO080749 Genomic DNA. Translation: CCD66409.1.
FO080749 Genomic DNA. Translation: CCD66410.1.
FO080749 Genomic DNA. Translation: CCD66411.1.
FO080749 Genomic DNA. Translation: CCD66412.1.
FO080749 Genomic DNA. Translation: CCD66413.1.
FO080749 Genomic DNA. Translation: CCD66414.1.
FO080749 Genomic DNA. Translation: CCD66415.1.
RefSeqiNP_001021014.1. NM_001025843.2. [Q9XZI6-3]
NP_001021015.1. NM_001025844.1. [Q9XZI6-9]
NP_001021016.1. NM_001025845.2. [Q9XZI6-2]
NP_491227.1. NM_058826.1.
NP_491228.1. NM_058827.3. [Q9XZI6-7]
NP_491229.1. NM_058828.1. [Q9XZI6-8]
NP_491230.1. NM_058829.3. [Q9XZI6-6]
UniGeneiCel.16650.

Genome annotation databases

EnsemblMetazoaiC32E8.10a; C32E8.10a; WBGene00006751. [Q9XZI6-1]
GeneIDi171952.
KEGGicel:CELE_C32E8.10.
UCSCiC32E8.10f. c. elegans. [Q9XZI6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF144257 mRNA. Translation: AAD37365.1 .
AF144258 mRNA. Translation: AAD37366.1 .
AF144259 mRNA. Translation: AAD37367.1 .
AF144260 mRNA. Translation: AAD37368.1 .
AF144261 mRNA. Translation: AAD37369.1 .
AF144262 mRNA. Translation: AAD37370.1 .
FO080749 Genomic DNA. Translation: CCD66409.1 .
FO080749 Genomic DNA. Translation: CCD66410.1 .
FO080749 Genomic DNA. Translation: CCD66411.1 .
FO080749 Genomic DNA. Translation: CCD66412.1 .
FO080749 Genomic DNA. Translation: CCD66413.1 .
FO080749 Genomic DNA. Translation: CCD66414.1 .
FO080749 Genomic DNA. Translation: CCD66415.1 .
RefSeqi NP_001021014.1. NM_001025843.2. [Q9XZI6-3 ]
NP_001021015.1. NM_001025844.1. [Q9XZI6-9 ]
NP_001021016.1. NM_001025845.2. [Q9XZI6-2 ]
NP_491227.1. NM_058826.1.
NP_491228.1. NM_058827.3. [Q9XZI6-7 ]
NP_491229.1. NM_058828.1. [Q9XZI6-8 ]
NP_491230.1. NM_058829.3. [Q9XZI6-6 ]
UniGenei Cel.16650.

3D structure databases

ProteinModelPortali Q9XZI6.
SMRi Q9XZI6. Positions 37-302.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 37426. 3 interactions.
DIPi DIP-25315N.
IntActi Q9XZI6. 7 interactions.
MINTi MINT-1053765.

Proteomic databases

PaxDbi Q9XZI6.
PRIDEi Q9XZI6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C32E8.10a ; C32E8.10a ; WBGene00006751 . [Q9XZI6-1 ]
GeneIDi 171952.
KEGGi cel:CELE_C32E8.10.
UCSCi C32E8.10f. c. elegans. [Q9XZI6-1 ]

Organism-specific databases

CTDi 171952.
WormBasei C32E8.10a ; CE23562 ; WBGene00006751 ; unc-11.
C32E8.10b ; CE27812 ; WBGene00006751 ; unc-11.
C32E8.10c ; CE24822 ; WBGene00006751 ; unc-11.
C32E8.10d ; CE23564 ; WBGene00006751 ; unc-11.
C32E8.10e ; CE23565 ; WBGene00006751 ; unc-11.
C32E8.10f ; CE23566 ; WBGene00006751 ; unc-11.
C32E8.10h ; CE27813 ; WBGene00006751 ; unc-11.

Phylogenomic databases

eggNOGi NOG267212.
GeneTreei ENSGT00390000008805.
InParanoidi Q9XZI6.
OMAi ITIHNIM.
OrthoDBi EOG76QFM8.
PhylomeDBi Q9XZI6.

Miscellaneous databases

NextBioi 873385.

Family and domain databases

Gene3Di 1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProi IPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
IPR001026. Epsin_dom_N.
[Graphical view ]
Pfami PF01417. ENTH. 1 hit.
[Graphical view ]
SMARTi SM00273. ENTH. 1 hit.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
PROSITEi PS50942. ENTH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles."
    Nonet M.L., Holgado A.M., Brewer F., Serpe C.J., Norbeck B.A., Holleran J., Wei L., Hartwieg E., Jorgensen E.M., Alfonso A.
    Mol. Biol. Cell 10:2343-2360(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), FUNCTION, SUBCELLULAR LOCATION.
    Strain: Bristol N21 Publication.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiPICAL_CAEEL
AccessioniPrimary (citable) accession number: Q9XZI6
Secondary accession number(s): Q95Q72
, Q95Q73, Q95Q74, Q95Q75, Q9UA04, Q9UA05, Q9XZI7, Q9XZI8, Q9XZI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3