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Q9XZI6

- PICAL_CAEEL

UniProt

Q9XZI6 - PICAL_CAEEL

Protein

Phosphatidylinositol-binding clathrin assembly protein unc-11

Gene

unc-11

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Required for the efficient targeting of the synaptic vesicle protein synaptobrevin.1 Publication

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: UniProtKB
    2. clathrin binding Source: WormBase
    3. protein binding Source: IntAct

    GO - Biological processi

    1. clathrin coat assembly Source: InterPro
    2. locomotion Source: WormBase
    3. positive regulation of necrotic cell death Source: WormBase
    4. post-embryonic development Source: WormBase
    5. receptor-mediated endocytosis Source: UniProtKB
    6. synaptic vesicle endocytosis Source: WormBase

    Keywords - Biological processi

    Endocytosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol-binding clathrin assembly protein unc-11
    Alternative name(s):
    AP180-like adaptor protein
    Uncoordinated protein 11
    Gene namesi
    Name:unc-11
    ORF Names:C32E8.10
    OrganismiCaenorhabditis elegansImported
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiC32E8.10a; CE23562; WBGene00006751; unc-11.
    C32E8.10b; CE27812; WBGene00006751; unc-11.
    C32E8.10c; CE24822; WBGene00006751; unc-11.
    C32E8.10d; CE23564; WBGene00006751; unc-11.
    C32E8.10e; CE23565; WBGene00006751; unc-11.
    C32E8.10f; CE23566; WBGene00006751; unc-11.
    C32E8.10h; CE27813; WBGene00006751; unc-11.

    Subcellular locationi

    Membraneclathrin-coated pit 1 Publication. Golgi apparatus 1 Publication. Cytoplasmic vesicleclathrin-coated vesicle 1 Publication
    Note: Colocalized with clathrin in the Golgi area.

    GO - Cellular componenti

    1. clathrin coat Source: InterPro
    2. clathrin-coated vesicle Source: UniProtKB-SubCell
    3. coated pit Source: UniProtKB
    4. Golgi apparatus Source: UniProtKB-SubCell
    5. presynaptic membrane Source: WormBase

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 586586Phosphatidylinositol-binding clathrin assembly protein unc-11PRO_0000187065Add
    BLAST

    Proteomic databases

    PaxDbiQ9XZI6.
    PRIDEiQ9XZI6.

    Expressioni

    Tissue specificityi

    High levels in the nervous system and at lower levels in other tissues. In neurons, enriched at presynaptic terminals and is also present in cell bodies.1 Publication

    Interactioni

    Subunit structurei

    Binds clathrin and phosphatidylinositol 4,5-bisphosphate.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    eya-1O176704EBI-311866,EBI-311862
    itsn-1Q9U2T93EBI-311866,EBI-2414252
    magu-1Q95XW53EBI-311866,EBI-2317194

    Protein-protein interaction databases

    BioGridi37426. 3 interactions.
    DIPiDIP-25315N.
    IntActiQ9XZI6. 7 interactions.
    MINTiMINT-1053765.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XZI6.
    SMRiQ9XZI6. Positions 37-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 161133ENTHCuratedPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PICALM/SNAP91 family.Curated
    Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG267212.
    GeneTreeiENSGT00390000008805.
    InParanoidiQ9XZI6.
    OMAiITIHNIM.
    OrthoDBiEOG76QFM8.
    PhylomeDBiQ9XZI6.

    Family and domain databases

    Gene3Di1.20.58.150. 1 hit.
    1.25.40.90. 1 hit.
    InterProiIPR014712. Clathrin_Pinositid-bd_GAT-like.
    IPR008942. ENTH_VHS.
    IPR013809. Epsin-like_N.
    IPR001026. Epsin_dom_N.
    [Graphical view]
    PfamiPF01417. ENTH. 1 hit.
    [Graphical view]
    SMARTiSM00273. ENTH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48464. SSF48464. 1 hit.
    PROSITEiPS50942. ENTH. 1 hit.
    [Graphical view]

    Sequences (9)i

    Sequence statusi: Complete.

    This entry describes 9 isoformsi produced by alternative splicing. Align

    Isoform A1 Publication (identifier: Q9XZI6-1) [UniParc]FASTAAdd to Basket

    Also known as: aCurated

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQTIEKALHQ PMPFTTGGQT ISDRLTAAKH SLAGSQLGKT ICKATTEEVM    50
    APKKKHLDYL LHCTNEPNVS IPSMANLLIE RTQNPNWTVV YKALITIHNI 100
    MCYGNERFSQ YLASCNTTFN LTAFVDKVGG AGGYDMSTHV RRYAKYIGEK 150
    INTYRMCAFD FCKVKRGRED GLLRTMHTDK LLKTIPILQN QIDALLEFSV 200
    TTSELNNGVI NCSFILLFRD LIRLFACYND GIINVLEKYF DMNKKQCRDA 250
    LDTYKSFLTR LDKVAEFLRV AESVGIDRGE IPDLTRAPAS LLEALEAHLI 300
    HLEGGKAPPP TQQHVAPHQF TTGFAFSQQP QPALGDAERQ RYIELEQERL 350
    RQFEDQKKSI NSANPFANDV ASAAPAPATS AAQPDLLDMF QSSAAPAPQT 400
    ADVTNPFGNF AAPSAFPTNV PPPAAHSAPF GVQPAPQHSA APFYANLHQA 450
    PPMQSQAPNG HQAAPFGYPN AHPDDLARMT AQMSLNQQGA PAGWNTTTSA 500
    VSNNPFGATS APQPMYTAPM GMYQQPFGAQ PMWNPAMAAY GQQYGYGQPV 550
    PPQQQHQIQL VHAAMAAKNA AQAQQAQAAS ADPFGL 586
    Length:586
    Mass (Da):64,064
    Last modified:November 1, 1999 - v1
    Checksum:i6E337A81D302DD81
    GO
    Isoform B1 Publication (identifier: Q9XZI6-2) [UniParc]FASTAAdd to Basket

    Also known as: hCurated

    The sequence of this isoform differs from the canonical sequence as follows:
         419-458: Missing.
         489-489: G → GYR

    Show »
    Length:548
    Mass (Da):60,273
    Checksum:i8641691EF297D717
    GO
    Isoform C1 Publication (identifier: Q9XZI6-3) [UniParc]FASTAAdd to Basket

    Also known as: bCurated

    The sequence of this isoform differs from the canonical sequence as follows:
         419-458: Missing.

    Show »
    Length:546
    Mass (Da):59,954
    Checksum:i69D424C56BAF517A
    GO
    Isoform c (identifier: Q9XZI6-7) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         459-459: N → NEPENPFITAPAAPQMHNAPPVPPPPASQGAPAPINPFADPSATAASSAQPFGDPDDFKFEQKNVKIKILIP

    Note: No experimental confirmation available.

    Show »
    Length:657
    Mass (Da):71,443
    Checksum:i891BA9FB3525AAD2
    GO
    Isoform D1 Publication (identifier: Q9XZI6-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         397-449: Missing.
         489-489: G → GYR

    Show »
    Length:535
    Mass (Da):58,978
    Checksum:i7EB8FC25A2896AA5
    GO
    Isoform d (identifier: Q9XZI6-8) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         397-408: APQTADVTNPFG → LLQCNRKLQMVS
         409-460: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:534
    Mass (Da):58,994
    Checksum:i4FDEE1FAEDBA4847
    GO
    Isoform E1 Publication (identifier: Q9XZI6-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         383-512: Missing.

    Show »
    Length:456
    Mass (Da):50,727
    Checksum:i44EDE64136212A9E
    GO
    Isoform e (identifier: Q9XZI6-9) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         383-467: QPDLLDMFQS...PNGHQAAPFG → HQCIRLQWEC...LVVYFSLSLS
         468-586: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:467
    Mass (Da):53,131
    Checksum:i42042675FC806DA9
    GO
    Isoform fCurated (identifier: Q9XZI6-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-388: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:198
    Mass (Da):20,632
    Checksum:i2F38F15C93F0F0C1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 388388Missing in isoform f. CuratedVSP_050679Add
    BLAST
    Alternative sequencei383 – 512130Missing in isoform E. 1 PublicationVSP_050680Add
    BLAST
    Alternative sequencei383 – 46785QPDLL…AAPFG → HQCIRLQWECINNHLVLSQC GILRWQHTVNNMDTDNLFHH NSNTKFNWFMQRWLQRTLHK LNRPRPPQPIHLDYSLVVYF SLSLS in isoform e. 1 PublicationVSP_020098Add
    BLAST
    Alternative sequencei397 – 44953Missing in isoform D. 1 PublicationVSP_050681Add
    BLAST
    Alternative sequencei397 – 40812APQTA…TNPFG → LLQCNRKLQMVS in isoform d. 1 PublicationVSP_020099Add
    BLAST
    Alternative sequencei409 – 46052Missing in isoform d. 1 PublicationVSP_020100Add
    BLAST
    Alternative sequencei419 – 45840Missing in isoform C and isoform B. 1 PublicationVSP_050682Add
    BLAST
    Alternative sequencei459 – 4591N → NEPENPFITAPAAPQMHNAP PVPPPPASQGAPAPINPFAD PSATAASSAQPFGDPDDFKF EQKNVKIKILIP in isoform c. 1 PublicationVSP_020101
    Alternative sequencei468 – 586119Missing in isoform e. 1 PublicationVSP_020102Add
    BLAST
    Alternative sequencei489 – 4891G → GYR in isoform B and isoform D. 1 PublicationVSP_050683

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF144257 mRNA. Translation: AAD37365.1.
    AF144258 mRNA. Translation: AAD37366.1.
    AF144259 mRNA. Translation: AAD37367.1.
    AF144260 mRNA. Translation: AAD37368.1.
    AF144261 mRNA. Translation: AAD37369.1.
    AF144262 mRNA. Translation: AAD37370.1.
    FO080749 Genomic DNA. Translation: CCD66409.1.
    FO080749 Genomic DNA. Translation: CCD66410.1.
    FO080749 Genomic DNA. Translation: CCD66411.1.
    FO080749 Genomic DNA. Translation: CCD66412.1.
    FO080749 Genomic DNA. Translation: CCD66413.1.
    FO080749 Genomic DNA. Translation: CCD66414.1.
    FO080749 Genomic DNA. Translation: CCD66415.1.
    RefSeqiNP_001021014.1. NM_001025843.2. [Q9XZI6-3]
    NP_001021015.1. NM_001025844.1. [Q9XZI6-9]
    NP_001021016.1. NM_001025845.2. [Q9XZI6-2]
    NP_491227.1. NM_058826.1.
    NP_491228.1. NM_058827.3. [Q9XZI6-7]
    NP_491229.1. NM_058828.1. [Q9XZI6-8]
    NP_491230.1. NM_058829.3. [Q9XZI6-6]
    UniGeneiCel.16650.

    Genome annotation databases

    EnsemblMetazoaiC32E8.10a; C32E8.10a; WBGene00006751. [Q9XZI6-1]
    GeneIDi171952.
    KEGGicel:CELE_C32E8.10.
    UCSCiC32E8.10f. c. elegans. [Q9XZI6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF144257 mRNA. Translation: AAD37365.1 .
    AF144258 mRNA. Translation: AAD37366.1 .
    AF144259 mRNA. Translation: AAD37367.1 .
    AF144260 mRNA. Translation: AAD37368.1 .
    AF144261 mRNA. Translation: AAD37369.1 .
    AF144262 mRNA. Translation: AAD37370.1 .
    FO080749 Genomic DNA. Translation: CCD66409.1 .
    FO080749 Genomic DNA. Translation: CCD66410.1 .
    FO080749 Genomic DNA. Translation: CCD66411.1 .
    FO080749 Genomic DNA. Translation: CCD66412.1 .
    FO080749 Genomic DNA. Translation: CCD66413.1 .
    FO080749 Genomic DNA. Translation: CCD66414.1 .
    FO080749 Genomic DNA. Translation: CCD66415.1 .
    RefSeqi NP_001021014.1. NM_001025843.2. [Q9XZI6-3 ]
    NP_001021015.1. NM_001025844.1. [Q9XZI6-9 ]
    NP_001021016.1. NM_001025845.2. [Q9XZI6-2 ]
    NP_491227.1. NM_058826.1.
    NP_491228.1. NM_058827.3. [Q9XZI6-7 ]
    NP_491229.1. NM_058828.1. [Q9XZI6-8 ]
    NP_491230.1. NM_058829.3. [Q9XZI6-6 ]
    UniGenei Cel.16650.

    3D structure databases

    ProteinModelPortali Q9XZI6.
    SMRi Q9XZI6. Positions 37-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 37426. 3 interactions.
    DIPi DIP-25315N.
    IntActi Q9XZI6. 7 interactions.
    MINTi MINT-1053765.

    Proteomic databases

    PaxDbi Q9XZI6.
    PRIDEi Q9XZI6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C32E8.10a ; C32E8.10a ; WBGene00006751 . [Q9XZI6-1 ]
    GeneIDi 171952.
    KEGGi cel:CELE_C32E8.10.
    UCSCi C32E8.10f. c. elegans. [Q9XZI6-1 ]

    Organism-specific databases

    CTDi 171952.
    WormBasei C32E8.10a ; CE23562 ; WBGene00006751 ; unc-11.
    C32E8.10b ; CE27812 ; WBGene00006751 ; unc-11.
    C32E8.10c ; CE24822 ; WBGene00006751 ; unc-11.
    C32E8.10d ; CE23564 ; WBGene00006751 ; unc-11.
    C32E8.10e ; CE23565 ; WBGene00006751 ; unc-11.
    C32E8.10f ; CE23566 ; WBGene00006751 ; unc-11.
    C32E8.10h ; CE27813 ; WBGene00006751 ; unc-11.

    Phylogenomic databases

    eggNOGi NOG267212.
    GeneTreei ENSGT00390000008805.
    InParanoidi Q9XZI6.
    OMAi ITIHNIM.
    OrthoDBi EOG76QFM8.
    PhylomeDBi Q9XZI6.

    Miscellaneous databases

    NextBioi 873385.

    Family and domain databases

    Gene3Di 1.20.58.150. 1 hit.
    1.25.40.90. 1 hit.
    InterProi IPR014712. Clathrin_Pinositid-bd_GAT-like.
    IPR008942. ENTH_VHS.
    IPR013809. Epsin-like_N.
    IPR001026. Epsin_dom_N.
    [Graphical view ]
    Pfami PF01417. ENTH. 1 hit.
    [Graphical view ]
    SMARTi SM00273. ENTH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48464. SSF48464. 1 hit.
    PROSITEi PS50942. ENTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles."
      Nonet M.L., Holgado A.M., Brewer F., Serpe C.J., Norbeck B.A., Holleran J., Wei L., Hartwieg E., Jorgensen E.M., Alfonso A.
      Mol. Biol. Cell 10:2343-2360(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E), FUNCTION, SUBCELLULAR LOCATION.
      Strain: Bristol N21 Publication.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiPICAL_CAEEL
    AccessioniPrimary (citable) accession number: Q9XZI6
    Secondary accession number(s): Q95Q72
    , Q95Q73, Q95Q74, Q95Q75, Q9UA04, Q9UA05, Q9XZI7, Q9XZI8, Q9XZI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3