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Q9XZE5

- PP2AA_DICDI

UniProt

Q9XZE5 - PP2AA_DICDI

Protein

Serine/threonine-protein phosphatase 2A catalytic subunit A

Gene

pho2a

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Plays a role in activating the myosin contractile function. Dephosphorylates threonine at 'Thr-1823', 'Thr-1833' and 'Thr-2029' in the C-terminal tail region of myosin II heavy chain (mhcA). Drives the assembly of dephosphorylated myosin II filaments to allow myosin recruitment into the cytoskeleton.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Manganese 1By similarity
    Metal bindingi56 – 561Manganese 1By similarity
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi114 – 1141Manganese 2By similarity
    Active sitei115 – 1151Proton donorBy similarity
    Metal bindingi164 – 1641Manganese 2By similarity
    Metal bindingi238 – 2381Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. myosin phosphatase activity Source: dictyBase
    3. protein serine/threonine phosphatase activity Source: dictyBase

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. myosin filament assembly Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A catalytic subunit A (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 2A 37 kDa catalytic subunit
    Gene namesi
    Name:pho2a
    Synonyms:PP2A, PP2Ac
    ORF Names:DDB_G0290263
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0290263. pho2a.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: dictyBase
    2. nuclear speck Source: UniProtKB-SubCell
    3. protein phosphatase type 2A complex Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306Serine/threonine-protein phosphatase 2A catalytic subunit APRO_0000368206Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei306 – 3061Leucine methyl esterBy similarity

    Post-translational modificationi

    Reversibly methyl esterified on Leu-306 by leucine carboxyl methyltransferase 1 (LCMT) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.By similarity

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    PP2A consists of a trimeric holoenzyme, composed of a 37 kDa catalytic subunit (C subunit) and a 65 kDa constant regulatory subunit (A subunit), that associates with a variety of regulatory subunits (B subunit) such as phr2AB (B55) and psrA (B56 homolog). The trimer may partially dissociates into a core 'AC' dimer equally active compared to the trimer.1 Publication

    Protein-protein interaction databases

    STRINGi44689.DDB_0191299.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XZE5.
    SMRiQ9XZE5. Positions 5-306.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    InParanoidiQ9XZE5.
    KOiK04382.
    OMAiQVKTLCD.
    PhylomeDBiQ9XZE5.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XZE5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEFQDVDKY ISILKECKPL SESEVRDLCE KAREILSKES NVQPVRCPVT    50
    VCGDIHGQFH DLMELFKIGG NCPDTNYLFM GDYVDRGFYS VETVTLLVAL 100
    KVRYKDRVTI LRGNHESRQI TQVYGFYDEC LRKYGNPNVW KLFTDLFDYL 150
    PLTALIENQV FCLHGGLSPS IDTLDHIENL DRVQEVPHEG AMCDLLWSDP 200
    DDRLGFGYSP RGAGYTFGKD ISEQFNHNNG LTLVARAHQL VMEGYNWCHD 250
    QNVVTIFSAP NYCYRCGNLA AIMEIDEKMK HTFLQFDPAP RRGEPHVTRR 300
    TPDYFL 306
    Length:306
    Mass (Da):35,186
    Last modified:November 1, 1999 - v1
    Checksum:i1DC85DC05E292FEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF138278 mRNA. Translation: AAD29693.1.
    AAFI02000162 Genomic DNA. Translation: EAL62258.1.
    RefSeqiXP_635791.1. XM_630699.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191299; DDB0191299; DDB_G0290263.
    GeneIDi8627596.
    KEGGiddi:DDB_G0290263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF138278 mRNA. Translation: AAD29693.1 .
    AAFI02000162 Genomic DNA. Translation: EAL62258.1 .
    RefSeqi XP_635791.1. XM_630699.1.

    3D structure databases

    ProteinModelPortali Q9XZE5.
    SMRi Q9XZE5. Positions 5-306.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191299 ; DDB0191299 ; DDB_G0290263 .
    GeneIDi 8627596.
    KEGGi ddi:DDB_G0290263.

    Organism-specific databases

    dictyBasei DDB_G0290263. pho2a.

    Phylogenomic databases

    eggNOGi COG0639.
    InParanoidi Q9XZE5.
    KOi K04382.
    OMAi QVKTLCD.
    PhylomeDBi Q9XZE5.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization and immunolocalization of Dictyostelium discoideum protein phosphatase 2A."
      Murphy M.B., Levi S.K., Egelhoff T.T.
      FEBS Lett. 456:7-12(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Biochemical characterization of a Dictyostelium myosin II heavy-chain phosphatase that promotes filament assembly."
      Murphy M.B., Egelhoff T.T.
      Eur. J. Biochem. 264:582-590(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    4. "The function of PP2A/B56 in non-metazoan multicellular development."
      Lee N.-S., Veeranki S., Kim B., Kim L.
      Differentiation 76:1104-1110(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPP2AA_DICDI
    AccessioniPrimary (citable) accession number: Q9XZE5
    Secondary accession number(s): Q54G92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3