Q9XZE5 (PP2AA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A catalytic subunit A
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2A 37 kDa catalytic subunit

Gene names

Name:	pho2a
Synonyms:	PP2A, PP2Ac
ORF Names:	DDB_G0290263

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in activating the myosin contractile function. Dephosphorylates threonine at 'Thr-1823', 'Thr-1833' and 'Thr-2029' in the C-terminal tail region of myosin II heavy chain (mhcA). Drives the assembly of dephosphorylated myosin II filaments to allow myosin recruitment into the cytoskeleton. Ref.3
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	PP2A consists of a trimeric holoenzyme, composed of a 37 kDa catalytic subunit (C subunit) and a 65 kDa constant regulatory subunit (A subunit), that associates with a variety of regulatory subunits (B subunit) such as phr2AB (B55) and psrA (B56 homolog). The trimer may partially dissociates into a core 'AC' dimer equally active compared to the trimer. Ref.3
Subcellular location	Cytoplasm › cytosol. Nucleus speckle Ref.1 Ref.3 Ref.4.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	myosin filament assembly Inferred from direct assay Ref.3. Source: dictyBase
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: dictyBase nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell protein phosphatase type 2A complex Inferred from direct assay Ref.3. Source: dictyBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW myosin phosphatase activity Inferred from direct assay Ref.3. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Serine/threonine-protein phosphatase 2A catalytic subunit A		PRO_0000368206
Sites
	Active site	115	1	Proton donor By similarity
	Metal binding	54	1	Iron By similarity
	Metal binding	56	1	Iron By similarity
	Metal binding	82	1	Iron By similarity
	Metal binding	82	1	Manganese By similarity
	Metal binding	114	1	Manganese By similarity
	Metal binding	164	1	Manganese By similarity
	Metal binding	238	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XZE5 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1DC85DC05E292FEE	FASTA	306	35,186
	10 20 30 40 50 60 MGEFQDVDKY ISILKECKPL SESEVRDLCE KAREILSKES NVQPVRCPVT VCGDIHGQFH 70 80 90 100 110 120 DLMELFKIGG NCPDTNYLFM GDYVDRGFYS VETVTLLVAL KVRYKDRVTI LRGNHESRQI 130 140 150 160 170 180 TQVYGFYDEC LRKYGNPNVW KLFTDLFDYL PLTALIENQV FCLHGGLSPS IDTLDHIENL 190 200 210 220 230 240 DRVQEVPHEG AMCDLLWSDP DDRLGFGYSP RGAGYTFGKD ISEQFNHNNG LTLVARAHQL 250 260 270 280 290 300 VMEGYNWCHD QNVVTIFSAP NYCYRCGNLA AIMEIDEKMK HTFLQFDPAP RRGEPHVTRR TPDYFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and immunolocalization of Dictyostelium discoideum protein phosphatase 2A." Murphy M.B., Levi S.K., Egelhoff T.T. FEBS Lett. 456:7-12(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Biochemical characterization of a Dictyostelium myosin II heavy-chain phosphatase that promotes filament assembly." Murphy M.B., Egelhoff T.T. Eur. J. Biochem. 264:582-590(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[4]	"The function of PP2A/B56 in non-metazoan multicellular development." Lee N.-S., Veeranki S., Kim B., Kim L. Differentiation 76:1104-1110(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF138278 mRNA. Translation: AAD29693.1. AAFI02000162 Genomic DNA. Translation: EAL62258.1.
RefSeq	XP_635791.1. XM_630699.1.
3D structure databases
HSSP	HSSP built from PDB template 1IT6 based on UniProtKB P36873.
ProteinModelPortal	Q9XZE5.
SMR	Q9XZE5. Positions 5-306.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191299.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191299; DDB0191299; DDB_G0290263.
GeneID	8627596.
KEGG	ddi:DDB_G0290263.
Organism-specific databases
dictyBase	DDB_G0290263. pho2a.
Phylogenomic databases
eggNOG	COG0639.
InParanoid	Q9XZE5.
KO	K04382.
OMA	TFNHANR.
ProtClustDB	CLSZ2429685.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2AA_DICDI

Accession

Primary (citable) accession number: Q9XZE5
Secondary accession number(s): Q54G92

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)