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Q9XZE5

- PP2AA_DICDI

UniProt

Q9XZE5 - PP2AA_DICDI

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Protein

Serine/threonine-protein phosphatase 2A catalytic subunit A

Gene
pho2a, PP2A, PP2Ac, DDB_G0290263
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in activating the myosin contractile function. Dephosphorylates threonine at 'Thr-1823', 'Thr-1833' and 'Thr-2029' in the C-terminal tail region of myosin II heavy chain (mhcA). Drives the assembly of dephosphorylated myosin II filaments to allow myosin recruitment into the cytoskeleton.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Manganese 1 By similarity
Metal bindingi56 – 561Manganese 1 By similarity
Metal bindingi82 – 821Manganese 1 By similarity
Metal bindingi82 – 821Manganese 2 By similarity
Metal bindingi114 – 1141Manganese 2 By similarity
Active sitei115 – 1151Proton donor By similarity
Metal bindingi164 – 1641Manganese 2 By similarity
Metal bindingi238 – 2381Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. myosin phosphatase activity Source: dictyBase
  3. protein serine/threonine phosphatase activity Source: dictyBase

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. myosin filament assembly Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A catalytic subunit A (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A 37 kDa catalytic subunit
Gene namesi
Name:pho2a
Synonyms:PP2A, PP2Ac
ORF Names:DDB_G0290263
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290263. pho2a.

Subcellular locationi

Cytoplasmcytosol. Nucleus speckle 3 Publications

GO - Cellular componenti

  1. cytosol Source: dictyBase
  2. nuclear speck Source: UniProtKB-SubCell
  3. protein phosphatase type 2A complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Serine/threonine-protein phosphatase 2A catalytic subunit APRO_0000368206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei306 – 3061Leucine methyl ester By similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-306 by leucine carboxyl methyltransferase 1 (LCMT) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

PP2A consists of a trimeric holoenzyme, composed of a 37 kDa catalytic subunit (C subunit) and a 65 kDa constant regulatory subunit (A subunit), that associates with a variety of regulatory subunits (B subunit) such as phr2AB (B55) and psrA (B56 homolog). The trimer may partially dissociates into a core 'AC' dimer equally active compared to the trimer.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0191299.

Structurei

3D structure databases

ProteinModelPortaliQ9XZE5.
SMRiQ9XZE5. Positions 5-306.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ9XZE5.
KOiK04382.
OMAiQVKTLCD.
PhylomeDBiQ9XZE5.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XZE5-1 [UniParc]FASTAAdd to Basket

« Hide

MGEFQDVDKY ISILKECKPL SESEVRDLCE KAREILSKES NVQPVRCPVT    50
VCGDIHGQFH DLMELFKIGG NCPDTNYLFM GDYVDRGFYS VETVTLLVAL 100
KVRYKDRVTI LRGNHESRQI TQVYGFYDEC LRKYGNPNVW KLFTDLFDYL 150
PLTALIENQV FCLHGGLSPS IDTLDHIENL DRVQEVPHEG AMCDLLWSDP 200
DDRLGFGYSP RGAGYTFGKD ISEQFNHNNG LTLVARAHQL VMEGYNWCHD 250
QNVVTIFSAP NYCYRCGNLA AIMEIDEKMK HTFLQFDPAP RRGEPHVTRR 300
TPDYFL 306
Length:306
Mass (Da):35,186
Last modified:November 1, 1999 - v1
Checksum:i1DC85DC05E292FEE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF138278 mRNA. Translation: AAD29693.1.
AAFI02000162 Genomic DNA. Translation: EAL62258.1.
RefSeqiXP_635791.1. XM_630699.1.

Genome annotation databases

EnsemblProtistsiDDB0191299; DDB0191299; DDB_G0290263.
GeneIDi8627596.
KEGGiddi:DDB_G0290263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF138278 mRNA. Translation: AAD29693.1 .
AAFI02000162 Genomic DNA. Translation: EAL62258.1 .
RefSeqi XP_635791.1. XM_630699.1.

3D structure databases

ProteinModelPortali Q9XZE5.
SMRi Q9XZE5. Positions 5-306.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191299.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191299 ; DDB0191299 ; DDB_G0290263 .
GeneIDi 8627596.
KEGGi ddi:DDB_G0290263.

Organism-specific databases

dictyBasei DDB_G0290263. pho2a.

Phylogenomic databases

eggNOGi COG0639.
InParanoidi Q9XZE5.
KOi K04382.
OMAi QVKTLCD.
PhylomeDBi Q9XZE5.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization and immunolocalization of Dictyostelium discoideum protein phosphatase 2A."
    Murphy M.B., Levi S.K., Egelhoff T.T.
    FEBS Lett. 456:7-12(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Biochemical characterization of a Dictyostelium myosin II heavy-chain phosphatase that promotes filament assembly."
    Murphy M.B., Egelhoff T.T.
    Eur. J. Biochem. 264:582-590(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  4. "The function of PP2A/B56 in non-metazoan multicellular development."
    Lee N.-S., Veeranki S., Kim B., Kim L.
    Differentiation 76:1104-1110(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPP2AA_DICDI
AccessioniPrimary (citable) accession number: Q9XZE5
Secondary accession number(s): Q54G92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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