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Protein

Ubiquitin carboxyl-terminal hydrolase

Gene

Uch-L5

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86NucleophileUniRule annotation1
Active sitei162Proton donorUniRule annotation1
Sitei177Important for enzyme activityUniRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein deubiquitination Source: FlyBase

Keywordsi

Molecular functionHydrolase, Protease, Thiol proteaseUniRule annotation
Biological processUbl conjugation pathwayUniRule annotation

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-209360. Ubiquitination and proteolysis of phosphorylated CI.
R-DME-209406. Degradation of NF-kappa-B inhibitor, CACT.
R-DME-209461. Ubiquitination and degradation of phosphorylated ARM.
R-DME-216167. Nuclear CI is degraded.
R-DME-432395. Degradation of TIM.
R-DME-432524. Degradation of PER.
R-DME-538848. Degradation of CLK.
R-DME-538864. Degradation of CRY.
R-DME-5689603. UCH proteinases.

Protein family/group databases

MEROPSiC12.A05.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolaseUniRule annotation (EC:3.4.19.12UniRule annotation)
Gene namesi
Name:Uch-L5Imported
Synonyms:BcDNA.LD24440Imported, BcDNA:LD24440Imported, Dmel\CG3431Imported, dUCH37Imported, l(3)j2B8Imported, p37AImported, Q9XZ61Imported, UCHImported, UCH 37Imported, Uch-L3Imported, uch-L3Imported, uch-l5Imported, Uch37Imported, Uchl3Imported, uchl3Imported
ORF Names:CG3431Imported, Dmel_CG3431Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0011327. Uch-L5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

ProteasomeImported

Expressioni

Gene expression databases

BgeeiFBgn0011327.

Interactioni

Protein-protein interaction databases

MINTiMINT-1767249.
STRINGi7227.FBpp0076200.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 208UCH_1InterPro annotationAdd BLAST201

Sequence similaritiesi

Belongs to the peptidase C12 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2778. Eukaryota.
ENOG410XP0P. LUCA.
GeneTreeiENSGT00510000046560.
HOGENOMiHOG000266301.
KOiK05610.
OMAiKDEDVYH.
OrthoDBiEOG091G0EO2.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiView protein in InterPro
IPR001578. Peptidase_C12_UCH.
IPR036959. Peptidase_C12_UCH_sf.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiView protein in Pfam
PF01088. Peptidase_C12. 1 hit.
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

Q9XZ61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDGAGNWCL IESDPGVFTE LIREFGCDGA QVEEIWSLDS ESFKNLEPIH
60 70 80 90 100
GLIFLFKWVQ EDEPAGKVVL DRENIFFAKQ VINNACATQA ILSLLMNLDH
110 120 130 140 150
EDIKLGETLT NFKEFCQCFD PYNKGLTLSN ASQIRTVHNS FARPTLFELD
160 170 180 190 200
TKNQKKDDDV YHFVGYMPIG GRLYELDGLR EGPIDLGEIK SEQNWIDVVR
210 220 230 240 250
PIIEKRMQRY SDGEIHFNLM ALISDRQRIY EQQIEKLLNP APNAMDTEED
260 270 280 290 300
RQAEISSLRT YIEYEIQKKK RYKVENVRRK HNYLPFIVEL LKILGENGQL
310 320
MPIYEKAKQR ALEREQAQRK KDTD
Length:324
Mass (Da):37,673
Last modified:November 1, 1999 - v1
Checksum:i7355CC51596CA298
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132567 mRNA. Translation: AAD27866.1.
AF145312 mRNA. Translation: AAF08393.1.
AE014296 Genomic DNA. Translation: AAF50257.1.
BT001294 mRNA. Translation: AAN71049.1.
AE014296 Genomic DNA. Translation: AGB94327.1.
RefSeqiNP_001261632.1. NM_001274703.1.
NP_524003.1. NM_079279.3.
UniGeneiDm.2640.

Genome annotation databases

EnsemblMetazoaiFBtr0076472; FBpp0076200; FBgn0011327.
FBtr0333867; FBpp0306000; FBgn0011327.
GeneIDi39102.
KEGGidme:Dmel_CG3431.
UCSCiCG3431-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiQ9XZ61_DROME
AccessioniPrimary (citable) accession number: Q9XZ61
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1999
Last sequence update: November 1, 1999
Last modified: November 22, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported