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Protein

Exostosin-3

Gene

botv

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Plays a central role in diffusion of morphogens hedgehog (hh), wingless (wg) and Decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of Hh, Dpp and wg morphogens.3 Publications

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei748SubstrateBy similarity1
Metal bindingi797Manganese; catalyticBy similarity1
Binding sitei825SubstrateBy similarity1
Active sitei886By similarity1

GO - Molecular functioni

GO - Biological processi

  • decapentaplegic signaling pathway Source: UniProtKB
  • glycosaminoglycan biosynthetic process Source: FlyBase
  • heparan sulfate proteoglycan biosynthetic process Source: FlyBase
  • heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: FlyBase
  • heparan sulfate proteoglycan metabolic process Source: FlyBase
  • heparin biosynthetic process Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • N-acetylglucosamine metabolic process Source: UniProtKB
  • smoothened signaling pathway Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.223. 1994.
2.4.1.224. 1994.
SignaLinkiQ9XZ08.
UniPathwayiUPA00756.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-3 (EC:2.4.1.223)
Alternative name(s):
Protein brother of tout-velu
Gene namesi
Name:botv
Synonyms:DEXT3
ORF Names:CG15110
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0027535. botv.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicSequence analysisAdd BLAST55
Transmembranei56 – 76Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini77 – 972LumenalSequence analysisAdd BLAST896

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi669P → L in botv510; induces defects in wing patterning due to impaired movement of morphogens. 1 Publication1
Mutagenesisi707G → D in botv8; induces defects in wing patterning due to impaired movement of morphogens. 1 Publication1
Mutagenesisi821G → D in botv514; induces defects in wing patterning due to impaired movement of morphogens. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496661 – 972Exostosin-3Add BLAST972

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi218N-linked (GlcNAc...)Sequence analysis1
Glycosylationi606N-linked (GlcNAc...)Sequence analysis1
Glycosylationi643N-linked (GlcNAc...)Sequence analysis1
Glycosylationi843N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi884 ↔ 932By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9XZ08.
PRIDEiQ9XZ08.

Expressioni

Tissue specificityi

In wing imaginal disk, it is ubiquitously expressed.1 Publication

Developmental stagei

Expressed throughout the developmental stages in both head and body from males and females.1 Publication

Gene expression databases

BgeeiFBgn0027535.
GenevisibleiQ9XZ08. DM.

Interactioni

Subunit structurei

Interacts with sau.1 Publication

Protein-protein interaction databases

BioGridi62863. 1 interactor.
IntActiQ9XZ08. 1 interactor.
STRINGi7227.FBpp0085763.

Structurei

3D structure databases

ProteinModelPortaliQ9XZ08.
SMRiQ9XZ08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni774 – 779Substrate bindingBy similarity6
Regioni795 – 797Substrate bindingBy similarity3
Regioni882 – 886Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2264. Eukaryota.
ENOG410XNPM. LUCA.
GeneTreeiENSGT00550000074496.
InParanoidiQ9XZ08.
KOiK02370.
OMAiRKSDTQN.
OrthoDBiEOG091G03XP.
PhylomeDBiQ9XZ08.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027674. EXO3/EXOL3.
IPR004263. Exostosin.
IPR015338. EXT_C.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062:SF73. PTHR11062:SF73. 2 hits.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XZ08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPAYDLGHS GEAYQPLDTG SGGGNEACAP NSSSAQIRHS MGFRTSWMRQ
60 70 80 90 100
FRRYKLPMVL LMLLFLVSCL AYRILSVEQD APPLDLHRSS PLLDAYEDFS
110 120 130 140 150
AMRAGDLKMR IEEMVRIKST VSVELRELES RRQKLQSDIS QYNQKIEELK
160 170 180 190 200
QELLREQTEL ERLKISVEQA QVAQREAVQR NTPDLALPRS LLPNTLPRKS
210 220 230 240 250
NPITGGMAAS CEMHNCFNHS RCSLTSGFPV YLYDPDEHSV QRKGYDIDGF
260 270 280 290 300
LKTTLKQTLG YNAHIVKDPK HACIYLVLVG EALLEQDLLR NNRYAAQEAE
310 320 330 340 350
HQQPSTPTLE NDCPVDMEKL YSLPYWGGDG RNHVLLNLAR RDLTSHRTNP
360 370 380 390 400
LYRQNTMRAI VVQSAFEREQ FRPGYDLIVP PILGPPGGDV WQECAEMVPA
410 420 430 440 450
RRKYLLTYQG ELRPKQSSLN PLDAFILEHL ADMAKGATQD QFVLQFQCVP
460 470 480 490 500
ATEQQEGDSL PDWTLCGSDS SRRQLLKDST FSLILPPLNG RVSSTLMLAR
510 520 530 540 550
IYEALRSGAV PVILGADELR LPYAETVDWR RTALLLPKAR ITELHFLLRA
560 570 580 590 600
VQDADLLLLR RQGRLIWERY LSSVQATVDT VIASLRDRLG IPPRPVPSVI
610 620 630 640 650
AQSVFNSTFI PLKSDPPVGL DTEPEESLGP IEPPYPSPAF RRNYTILRMQ
660 670 680 690 700
AKEAWNDWLD PFYLYPQLPF DPALPSEAKF MGSHTGFRPI GKGLGGAGKE
710 720 730 740 750
FGESLGGNYP REQFTIVMLT YEREQVLMDS LGRLYGLPYL HKVVVVWNSP
760 770 780 790 800
KPPLDDLRWP DIGVPVAVLR APRNSLNNRF LPFDVIETEA VLSVDDDAHL
810 820 830 840 850
RHDEILFGFR VWREHRDRVV GFPGRYHAWD LGNPNGQWHY NSNYSCELSM
860 870 880 890 900
VLTGAAFVHK YYLYLYTYHL PQAIRDKVDE YMNCEDIAMN FLVSHITRKP
910 920 930 940 950
PVKVTSRWTF RCPGCPVSLS EDDTHFQERH KCINFFSRVF GYTPLLNTQY
960 970
RADSILFKTR IPHDKQKCFK YI
Length:972
Mass (Da):111,000
Last modified:November 1, 1999 - v1
Checksum:i2A111BD6912FFCCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077850 mRNA. Translation: BAB89399.1.
AE013599 Genomic DNA. Translation: AAF57601.1.
AF132161 mRNA. Translation: AAD34749.1.
RefSeqiNP_523790.1. NM_079066.3.
UniGeneiDm.3207.

Genome annotation databases

EnsemblMetazoaiFBtr0086579; FBpp0085763; FBgn0027535.
GeneIDi37198.
KEGGidme:Dmel_CG15110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077850 mRNA. Translation: BAB89399.1.
AE013599 Genomic DNA. Translation: AAF57601.1.
AF132161 mRNA. Translation: AAD34749.1.
RefSeqiNP_523790.1. NM_079066.3.
UniGeneiDm.3207.

3D structure databases

ProteinModelPortaliQ9XZ08.
SMRiQ9XZ08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62863. 1 interactor.
IntActiQ9XZ08. 1 interactor.
STRINGi7227.FBpp0085763.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Proteomic databases

PaxDbiQ9XZ08.
PRIDEiQ9XZ08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086579; FBpp0085763; FBgn0027535.
GeneIDi37198.
KEGGidme:Dmel_CG15110.

Organism-specific databases

CTDi37198.
FlyBaseiFBgn0027535. botv.

Phylogenomic databases

eggNOGiKOG2264. Eukaryota.
ENOG410XNPM. LUCA.
GeneTreeiENSGT00550000074496.
InParanoidiQ9XZ08.
KOiK02370.
OMAiRKSDTQN.
OrthoDBiEOG091G03XP.
PhylomeDBiQ9XZ08.

Enzyme and pathway databases

UniPathwayiUPA00756.
BRENDAi2.4.1.223. 1994.
2.4.1.224. 1994.
SignaLinkiQ9XZ08.

Miscellaneous databases

GenomeRNAii37198.
PROiQ9XZ08.

Gene expression databases

BgeeiFBgn0027535.
GenevisibleiQ9XZ08. DM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027674. EXO3/EXOL3.
IPR004263. Exostosin.
IPR015338. EXT_C.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062:SF73. PTHR11062:SF73. 2 hits.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEXT3_DROME
AccessioniPrimary (citable) accession number: Q9XZ08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.