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UniProtKB - Q9XYU1 (MCM3_DROME)

UniProt

Q9XYU1 - MCM3_DROME

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Protein

DNA replication licensing factor Mcm3

Gene

Mcm3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3478ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA replication initiation Source: InterPro
  • mitotic G2 DNA damage checkpoint Source: FlyBase
  • neurogenesis Source: FlyBase
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor Mcm3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 3 protein
Short name:
DmMCM3
Gene namesi
Name:Mcm3
Synonyms:Mcm3-RA
ORF Names:CG4206
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora ›
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0024332. Mcm3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi346 – 3461K → A: Greatly reduces complex helicase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 819819DNA replication licensing factor Mcm3PRO_0000406421Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei522 – 5221Phosphoserine1 Publication
Modified residuei538 – 5381Phosphotyrosine1 Publication
Modified residuei664 – 6641Phosphoserine1 Publication
Modified residuei666 – 6661Phosphoserine1 Publication
Modified residuei680 – 6801Phosphoserine1 Publication
Modified residuei682 – 6821Phosphoserine1 Publication
Modified residuei690 – 6901Phosphothreonine1 Publication
Modified residuei692 – 6921Phosphothreonine1 Publication
Modified residuei697 – 6971Phosphoserine2 Publications
Modified residuei735 – 7351Phosphoserine1 Publication
Modified residuei739 – 7391Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9XYU1.
PRIDEiQ9XYU1.

PTM databases

iPTMnetiQ9XYU1.

Expressioni

Gene expression databases

BgeeiFBgn0024332.
GenevisibleiQ9XYU1. DM.

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mcm5Q9VGW63EBI-103930,EBI-83298

Protein-protein interaction databases

BioGridi57959. 5 interactions.
IntActiQ9XYU1. 4 interactions.
MINTiMINT-990984.
STRINGi7227.FBpp0070729.

Structurei

3D structure databases

ProteinModelPortaliQ9XYU1.
SMRiQ9XYU1. Positions 43-646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini290 – 496207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi472 – 4754Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000075022.
InParanoidiQ9XYU1.
KOiK02541.
OMAiLGSMVCV.
OrthoDBiEOG091G02B0.
PhylomeDBiQ9XYU1.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XYU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHEGEQFIK DIQREYVDFL DDEEDQGIYA GHVKDMIAEK SKRLIVNVND 
        60         70         80         90        100
LKRKNPQRAL GLLSNAADEQ LAFGRALKEY ASTVDPGYAK MHEDLFVGFE 
       110        120        130        140        150
GCFGNRHVTP RSLTSIYLGN MVCVEGIVTK VSLIRPKVVR SVHYCPNTRK 
       160        170        180        190        200
VMERKYTDLT SFEAVPSGAA YPTKDEDGNL LETEYGLSVY KDHQTLTIQE 
       210        220        230        240        250
MPEKAPAGQL PRSVDIVCDD DLVDRCKPGD RVQIVGSYRC LPGKRGGYTS 
       260        270        280        290        300
GTFRTVLLAN NISLLSKESN LDISREDIML CKKLAKNNDI FELLSKSLAP 
       310        320        330        340        350
SIHGHAYVKQ AILCLLLGGV EKILPNGTRL RGDINVLLIG DPSVAKSQLL 
       360        370        380        390        400
RYVLNTAPRA IPTTGRGSSG VGLTAAVTTD QETGERRLEA GAMVLADRGV 
       410        420        430        440        450
VCIDEFDKMS DIDRTAIHEV MEQGRVTISK AGIHASLNAR CSVLAAANPV 
       460        470        480        490        500
YGRYDQYKTP MENIGLQDSL LSRFDLLFVM LDVIDSDVDQ MISDHVVRMH 
       510        520        530        540        550
RYRNPKEADG EPLSMGSSYA DSLSFVSSSE EKKDTEVYEK YDALLHGKSR 
       560        570        580        590        600
QRHEKILSVE FMRKYIHIAK CMKPKLGEQA CEAIANEYSR LRSQEAVETD 
       610        620        630        640        650
VARTQPITAR TLETLIRLST AHARARMSKS VTIDDAHAAI ELVQFAYFKK 
       660        670        680        690        700
VLDKDRPSKR RRNSGSDAED DNGEASSQRS PSRRSKRTRT ATVGADSDEE 
       710        720        730        740        750
DIEPPQPDAG DLTRRETRRS LPARSVAMLM ASPSSEEQSV ATSTTEPAII 
       760        770        780        790        800
SDARLGEFKN NLQRLFREAR EQSLALARIT TAINVGSQEP FTAGEIEAAV 
       810 
HRMTEDNQIM VADDIVFLI
Length:819
Mass (Da):90,918
Last modified:November 1, 1999 - v1
Checksum:iCE4C14FE89BA8113
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti141 – 1411S → C in BAA34731 (PubMed:9795205).Curated
Sequence conflicti193 – 1931H → D in BAA34731 (PubMed:9795205).Curated
Sequence conflicti264 – 2641L → Q in BAA34731 (PubMed:9795205).Curated
Sequence conflicti551 – 5511Q → L in BAA34731 (PubMed:9795205).Curated
Sequence conflicti597 – 5971V → D in BAA34731 (PubMed:9795205).Curated
Sequence conflicti720 – 7201S → L in BAA34731 (PubMed:9795205).Curated
Sequence conflicti795 – 7962EI → AL in BAA34731 (PubMed:9795205).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010107 mRNA. Translation: BAA34731.1.
AF124745 mRNA. Translation: AAD32859.1.
AE014298 Genomic DNA. Translation: AAF46023.1.
BT046156 mRNA. Translation: ACI46544.1.
RefSeqiNP_511048.2. NM_078493.4.
UniGeneiDm.2020.

Genome annotation databases

EnsemblMetazoaiFBtr0070762; FBpp0070729; FBgn0024332.
GeneIDi31449.
KEGGidme:Dmel_CG4206.
UCSCiCG4206-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010107 mRNA. Translation: BAA34731.1.
AF124745 mRNA. Translation: AAD32859.1.
AE014298 Genomic DNA. Translation: AAF46023.1.
BT046156 mRNA. Translation: ACI46544.1.
RefSeqiNP_511048.2. NM_078493.4.
UniGeneiDm.2020.

3D structure databases

ProteinModelPortaliQ9XYU1.
SMRiQ9XYU1. Positions 43-646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57959. 5 interactions.
IntActiQ9XYU1. 4 interactions.
MINTiMINT-990984.
STRINGi7227.FBpp0070729.

PTM databases

iPTMnetiQ9XYU1.

Proteomic databases

PaxDbiQ9XYU1.
PRIDEiQ9XYU1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070762; FBpp0070729; FBgn0024332.
GeneIDi31449.
KEGGidme:Dmel_CG4206.
UCSCiCG4206-RA. d. melanogaster.

Organism-specific databases

CTDi4172.
FlyBaseiFBgn0024332. Mcm3.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000075022.
InParanoidiQ9XYU1.
KOiK02541.
OMAiLGSMVCV.
OrthoDBiEOG091G02B0.
PhylomeDBiQ9XYU1.

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Miscellaneous databases

GenomeRNAii31449.
PROiQ9XYU1.

Gene expression databases

BgeeiFBgn0024332.
GenevisibleiQ9XYU1. DM.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM3_DROME
AccessioniPrimary (citable) accession number: Q9XYU1
Secondary accession number(s): P91675
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.