UniProtKB - Q9XYU1 (MCM3_DROME)
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Protein
DNA replication licensing factor Mcm3
Gene
Mcm3
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Acts as component of the Mcm2-7 complex (Mcm complex) (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.2 Publications
Catalytic activityi
ATP + H2O = ADP + phosphate.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 340 – 347 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- helicase activity Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- DNA replication initiation Source: InterPro
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-176187. Activation of ATR in response to replication stress. R-DME-68867. Assembly of the pre-replicative complex. R-DME-68949. Orc1 removal from chromatin. R-DME-68962. Activation of the pre-replicative complex. R-DME-69052. Switching of origins to a post-replicative state. R-DME-69300. Removal of licensing factors from origins. |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor Mcm3 (EC:3.6.4.12)Alternative name(s): Minichromosome maintenance 3 protein Short name: DmMCM3 |
Gene namesi | Name:Mcm3 Synonyms:Mcm3-RA ORF Names:CG4206 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0284442. Mcm3. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 346 | K → A: Greatly reduces complex helicase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000406421 | 1 – 819 | DNA replication licensing factor Mcm3Add BLAST | 819 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 522 | Phosphoserine1 Publication | 1 | |
Modified residuei | 538 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 664 | Phosphoserine1 Publication | 1 | |
Modified residuei | 666 | Phosphoserine1 Publication | 1 | |
Modified residuei | 680 | Phosphoserine1 Publication | 1 | |
Modified residuei | 682 | Phosphoserine1 Publication | 1 | |
Modified residuei | 690 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 692 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 697 | Phosphoserine2 Publications | 1 | |
Modified residuei | 735 | Phosphoserine1 Publication | 1 | |
Modified residuei | 739 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9XYU1. |
PRIDEi | Q9XYU1. |
PTM databases
iPTMneti | Q9XYU1. |
Interactioni
Subunit structurei
Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Mcm5 | Q9VGW6 | 7 | EBI-103930,EBI-83298 |
Protein-protein interaction databases
BioGridi | 57959. 8 interactors. |
IntActi | Q9XYU1. 11 interactors. |
STRINGi | 7227.FBpp0070729. |
Structurei
3D structure databases
ProteinModelPortali | Q9XYU1. |
SMRi | Q9XYU1. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 290 – 496 | MCMAdd BLAST | 207 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 472 – 475 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0479. Eukaryota. COG1241. LUCA. |
GeneTreei | ENSGT00900000141082. |
InParanoidi | Q9XYU1. |
KOi | K02541. |
OMAi | YQSKVRE. |
OrthoDBi | EOG091G02B0. |
PhylomeDBi | Q9XYU1. |
Family and domain databases
InterProi | View protein in InterPro IPR003593. AAA+_ATPase. IPR031327. MCM. IPR008046. Mcm3. IPR018525. MCM_CS. IPR001208. MCM_dom. IPR027925. MCM_N. IPR033762. MCM_OB. IPR012340. NA-bd_OB-fold. IPR027417. P-loop_NTPase. |
PANTHERi | PTHR11630. PTHR11630. 1 hit. |
Pfami | View protein in Pfam PF00493. MCM. 1 hit. PF14551. MCM_N. 1 hit. PF17207. MCM_OB. 1 hit. |
PRINTSi | PR01657. MCMFAMILY. PR01659. MCMPROTEIN3. |
SMARTi | View protein in SMART SM00382. AAA. 1 hit. SM00350. MCM. 1 hit. |
SUPFAMi | SSF50249. SSF50249. 1 hit. SSF52540. SSF52540. 2 hits. |
PROSITEi | View protein in PROSITE PS00847. MCM_1. 1 hit. PS50051. MCM_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q9XYU1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAHEGEQFIK DIQREYVDFL DDEEDQGIYA GHVKDMIAEK SKRLIVNVND
60 70 80 90 100
LKRKNPQRAL GLLSNAADEQ LAFGRALKEY ASTVDPGYAK MHEDLFVGFE
110 120 130 140 150
GCFGNRHVTP RSLTSIYLGN MVCVEGIVTK VSLIRPKVVR SVHYCPNTRK
160 170 180 190 200
VMERKYTDLT SFEAVPSGAA YPTKDEDGNL LETEYGLSVY KDHQTLTIQE
210 220 230 240 250
MPEKAPAGQL PRSVDIVCDD DLVDRCKPGD RVQIVGSYRC LPGKRGGYTS
260 270 280 290 300
GTFRTVLLAN NISLLSKESN LDISREDIML CKKLAKNNDI FELLSKSLAP
310 320 330 340 350
SIHGHAYVKQ AILCLLLGGV EKILPNGTRL RGDINVLLIG DPSVAKSQLL
360 370 380 390 400
RYVLNTAPRA IPTTGRGSSG VGLTAAVTTD QETGERRLEA GAMVLADRGV
410 420 430 440 450
VCIDEFDKMS DIDRTAIHEV MEQGRVTISK AGIHASLNAR CSVLAAANPV
460 470 480 490 500
YGRYDQYKTP MENIGLQDSL LSRFDLLFVM LDVIDSDVDQ MISDHVVRMH
510 520 530 540 550
RYRNPKEADG EPLSMGSSYA DSLSFVSSSE EKKDTEVYEK YDALLHGKSR
560 570 580 590 600
QRHEKILSVE FMRKYIHIAK CMKPKLGEQA CEAIANEYSR LRSQEAVETD
610 620 630 640 650
VARTQPITAR TLETLIRLST AHARARMSKS VTIDDAHAAI ELVQFAYFKK
660 670 680 690 700
VLDKDRPSKR RRNSGSDAED DNGEASSQRS PSRRSKRTRT ATVGADSDEE
710 720 730 740 750
DIEPPQPDAG DLTRRETRRS LPARSVAMLM ASPSSEEQSV ATSTTEPAII
760 770 780 790 800
SDARLGEFKN NLQRLFREAR EQSLALARIT TAINVGSQEP FTAGEIEAAV
810
HRMTEDNQIM VADDIVFLI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 141 | S → C in BAA34731 (PubMed:9795205).Curated | 1 | |
Sequence conflicti | 193 | H → D in BAA34731 (PubMed:9795205).Curated | 1 | |
Sequence conflicti | 264 | L → Q in BAA34731 (PubMed:9795205).Curated | 1 | |
Sequence conflicti | 551 | Q → L in BAA34731 (PubMed:9795205).Curated | 1 | |
Sequence conflicti | 597 | V → D in BAA34731 (PubMed:9795205).Curated | 1 | |
Sequence conflicti | 720 | S → L in BAA34731 (PubMed:9795205).Curated | 1 | |
Sequence conflicti | 795 – 796 | EI → AL in BAA34731 (PubMed:9795205).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB010107 mRNA. Translation: BAA34731.1. AF124745 mRNA. Translation: AAD32859.1. AE014298 Genomic DNA. Translation: AAF46023.1. BT046156 mRNA. Translation: ACI46544.1. |
RefSeqi | NP_511048.2. NM_078493.4. |
UniGenei | Dm.2020. |
Genome annotation databases
EnsemblMetazoai | FBtr0070762; FBpp0070729; FBgn0284442. |
GeneIDi | 31449. |
KEGGi | dme:Dmel_CG4206. |
UCSCi | CG4206-RA. d. melanogaster. |
Similar proteinsi
Entry informationi
Entry namei | MCM3_DROME | |
Accessioni | Q9XYU1Primary (citable) accession number: Q9XYU1 Secondary accession number(s): P91675 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 5, 2011 |
Last sequence update: | November 1, 1999 | |
Last modified: | March 28, 2018 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |