Mcm3

Functionⁱ

Acts as component of the Mcm2-7 complex (Mcm complex) (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.2 Publications

Catalytic activityⁱ

ATP + H₂O = ADP + phosphate.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	340 – 347	ATPSequence analysis		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
DNA binding Source: UniProtKB-KW
helicase activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
DNA replication initiation Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Helicase, Hydrolase
Biological process	Cell cycle, DNA replication
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-DME-176187 Activation of ATR in response to replication stress R-DME-68867 Assembly of the pre-replicative complex R-DME-68949 Orc1 removal from chromatin R-DME-68962 Activation of the pre-replicative complex R-DME-69052 Switching of origins to a post-replicative state

Reactomeⁱ

R-DME-176187 Activation of ATR in response to replication stress
R-DME-68867 Assembly of the pre-replicative complex
R-DME-68949 Orc1 removal from chromatin
R-DME-68962 Activation of the pre-replicative complex
R-DME-69052 Switching of origins to a post-replicative state

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA replication licensing factor Mcm3 (EC:3.6.4.12) Alternative name(s): Minichromosome maintenance 3 protein Short name: DmMCM3
Gene namesⁱ	Name:Mcm3 Synonyms:Mcm3-RA ORF Names:CG4206
Organismⁱ	Drosophila melanogaster (Fruit fly)
Taxonomic identifierⁱ	7227 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophila › Sophophora › melanogaster group › melanogaster subgroup
Proteomesⁱ	UP000000803 Componentⁱ: Chromosome X

Organism-specific databases

Drosophila genome database More...FlyBaseⁱ	FBgn0284442 Mcm3

FlyBaseⁱ

FBgn0284442 Mcm3

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	346	K → A: Greatly reduces complex helicase activity. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000406421	1 – 819	DNA replication licensing factor Mcm3Add BLAST		819

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	522	Phosphoserine1 Publication	1
Modified residueⁱ	538	Phosphotyrosine1 Publication	1
Modified residueⁱ	664	Phosphoserine1 Publication	1
Modified residueⁱ	666	Phosphoserine1 Publication	1
Modified residueⁱ	680	Phosphoserine1 Publication	1
Modified residueⁱ	682	Phosphoserine1 Publication	1
Modified residueⁱ	690	Phosphothreonine1 Publication	1
Modified residueⁱ	692	Phosphothreonine1 Publication	1
Modified residueⁱ	697	Phosphoserine2 Publications	1
Modified residueⁱ	735	Phosphoserine1 Publication	1
Modified residueⁱ	739	Phosphoserine1 Publication	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9XYU1
PRIDEⁱ	Q9XYU1

PTM databases

iPTMnetⁱ	Q9XYU1

iPTMnetⁱ

Q9XYU1

Expressionⁱ

Gene expression databases

Bgeeⁱ	FBgn0024332
ExpressionAtlasⁱ	Q9XYU1 differential
Genevisibleⁱ	Q9XYU1 DM

Interactionⁱ

Subunit structureⁱ

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Mcm5	Q9VGW6	7	EBI-103930,EBI-83298

With

Entry

#Exp.

IntAct

Notes

Mcm5

Q9VGW6

7

EBI-103930,EBI-83298

Protein-protein interaction databases

BioGridⁱ	57959, 8 interactors
IntActⁱ	Q9XYU1, 11 interactors
STRINGⁱ	7227.FBpp0070729

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9XYU1
SMRⁱ	Q9XYU1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	290 – 496	MCMAdd BLAST		207

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	472 – 475	Arginine finger		4

Sequence similaritiesⁱ

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0479 Eukaryota COG1241 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000141082
InParanoidⁱ	Q9XYU1
KEGG Orthology (KO) More...KOⁱ	K02541
OMAⁱ	YQSKVRE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02B0
PhylomeDBⁱ	Q9XYU1

Family and domain databases

InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR031327 MCM IPR008046 Mcm3 IPR018525 MCM_CS IPR001208 MCM_dom IPR027925 MCM_N IPR033762 MCM_OB IPR012340 NA-bd_OB-fold IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR11630 PTHR11630, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00493 MCM, 1 hit PF14551 MCM_N, 1 hit PF17207 MCM_OB, 1 hit
PRINTSⁱ	PR01657 MCMFAMILY PR01659 MCMPROTEIN3
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit SM00350 MCM, 1 hit
SUPFAMⁱ	SSF50249 SSF50249, 1 hit SSF52540 SSF52540, 2 hits
PROSITEⁱ	View protein in PROSITE PS00847 MCM_1, 1 hit PS50051 MCM_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9XYU1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAHEGEQFIK DIQREYVDFL DDEEDQGIYA GHVKDMIAEK SKRLIVNVND 
        60         70         80         90        100
LKRKNPQRAL GLLSNAADEQ LAFGRALKEY ASTVDPGYAK MHEDLFVGFE 
       110        120        130        140        150
GCFGNRHVTP RSLTSIYLGN MVCVEGIVTK VSLIRPKVVR SVHYCPNTRK 
       160        170        180        190        200
VMERKYTDLT SFEAVPSGAA YPTKDEDGNL LETEYGLSVY KDHQTLTIQE 
       210        220        230        240        250
MPEKAPAGQL PRSVDIVCDD DLVDRCKPGD RVQIVGSYRC LPGKRGGYTS 
       260        270        280        290        300
GTFRTVLLAN NISLLSKESN LDISREDIML CKKLAKNNDI FELLSKSLAP 
       310        320        330        340        350
SIHGHAYVKQ AILCLLLGGV EKILPNGTRL RGDINVLLIG DPSVAKSQLL 
       360        370        380        390        400
RYVLNTAPRA IPTTGRGSSG VGLTAAVTTD QETGERRLEA GAMVLADRGV 
       410        420        430        440        450
VCIDEFDKMS DIDRTAIHEV MEQGRVTISK AGIHASLNAR CSVLAAANPV 
       460        470        480        490        500
YGRYDQYKTP MENIGLQDSL LSRFDLLFVM LDVIDSDVDQ MISDHVVRMH 
       510        520        530        540        550
RYRNPKEADG EPLSMGSSYA DSLSFVSSSE EKKDTEVYEK YDALLHGKSR 
       560        570        580        590        600
QRHEKILSVE FMRKYIHIAK CMKPKLGEQA CEAIANEYSR LRSQEAVETD 
       610        620        630        640        650
VARTQPITAR TLETLIRLST AHARARMSKS VTIDDAHAAI ELVQFAYFKK 
       660        670        680        690        700
VLDKDRPSKR RRNSGSDAED DNGEASSQRS PSRRSKRTRT ATVGADSDEE 
       710        720        730        740        750
DIEPPQPDAG DLTRRETRRS LPARSVAMLM ASPSSEEQSV ATSTTEPAII 
       760        770        780        790        800
SDARLGEFKN NLQRLFREAR EQSLALARIT TAINVGSQEP FTAGEIEAAV 
       810 
HRMTEDNQIM VADDIVFLI

Length:819

Mass (Da):90,918

Last modified:November 1, 1999 - v1

Checksum:ⁱCE4C14FE89BA8113

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	141	S → C in BAA34731 (PubMed:9795205).Curated	1
Sequence conflictⁱ	193	H → D in BAA34731 (PubMed:9795205).Curated	1
Sequence conflictⁱ	264	L → Q in BAA34731 (PubMed:9795205).Curated	1
Sequence conflictⁱ	551	Q → L in BAA34731 (PubMed:9795205).Curated	1
Sequence conflictⁱ	597	V → D in BAA34731 (PubMed:9795205).Curated	1
Sequence conflictⁱ	720	S → L in BAA34731 (PubMed:9795205).Curated	1
Sequence conflictⁱ	795 – 796	EI → AL in BAA34731 (PubMed:9795205).Curated	2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB010107 mRNA Translation: BAA34731.1 AF124745 mRNA Translation: AAD32859.1 AE014298 Genomic DNA Translation: AAF46023.1 BT046156 mRNA Translation: ACI46544.1
NCBI Reference Sequences More...RefSeqⁱ	NP_511048.2, NM_078493.4
UniGeneⁱ	Dm.2020

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0070762; FBpp0070729; FBgn0284442
GeneIDⁱ	31449
KEGGⁱ	dme:Dmel_CG4206
UCSC genome browser More...UCSCⁱ	CG4206-RA d. melanogaster

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q9XYU1	UPI000B9FC02E		DROOB		832
UPI0007E77F3D		DROMI		827
DNA helicase		DROPS		826
UPI0007E7280E		DROTK		824
UPI0007E36600		DROSZ		822
+15

Protein	Similar proteins		Species	Score	Length
Q9XYU1	UPI000B9FC02E		DROOB		832
UPI0007E77F3D		DROMI		827
DNA helicase		DROPS		826
UPI0007E7280E		DROTK		824
UPI0007E36600		DROSZ		822
+162

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB010107 mRNA Translation: BAA34731.1 AF124745 mRNA Translation: AAD32859.1 AE014298 Genomic DNA Translation: AAF46023.1 BT046156 mRNA Translation: ACI46544.1
NCBI Reference Sequences More...RefSeqⁱ	NP_511048.2, NM_078493.4
UniGeneⁱ	Dm.2020

3D structure databases

ProteinModelPortalⁱ	Q9XYU1
SMRⁱ	Q9XYU1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	57959, 8 interactors
IntActⁱ	Q9XYU1, 11 interactors
STRINGⁱ	7227.FBpp0070729

PTM databases

iPTMnetⁱ	Q9XYU1

iPTMnetⁱ

Q9XYU1

Proteomic databases

PaxDbⁱ	Q9XYU1
PRIDEⁱ	Q9XYU1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0070762; FBpp0070729; FBgn0284442
GeneIDⁱ	31449
KEGGⁱ	dme:Dmel_CG4206
UCSC genome browser More...UCSCⁱ	CG4206-RA d. melanogaster

Organism-specific databases

CTDⁱ	4172
Drosophila genome database More...FlyBaseⁱ	FBgn0284442 Mcm3

Phylogenomic databases

eggNOGⁱ	KOG0479 Eukaryota COG1241 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000141082
InParanoidⁱ	Q9XYU1
KEGG Orthology (KO) More...KOⁱ	K02541
OMAⁱ	YQSKVRE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02B0
PhylomeDBⁱ	Q9XYU1

Enzyme and pathway databases

Reactomeⁱ	R-DME-176187 Activation of ATR in response to replication stress R-DME-68867 Assembly of the pre-replicative complex R-DME-68949 Orc1 removal from chromatin R-DME-68962 Activation of the pre-replicative complex R-DME-69052 Switching of origins to a post-replicative state

Reactomeⁱ

R-DME-176187 Activation of ATR in response to replication stress
R-DME-68867 Assembly of the pre-replicative complex
R-DME-68949 Orc1 removal from chromatin
R-DME-68962 Activation of the pre-replicative complex
R-DME-69052 Switching of origins to a post-replicative state

Miscellaneous databases

GenomeRNAiⁱ	31449
Protein Ontology More...PROⁱ	PR:Q9XYU1

Gene expression databases

Bgeeⁱ	FBgn0024332
ExpressionAtlasⁱ	Q9XYU1 differential
Genevisibleⁱ	Q9XYU1 DM

Family and domain databases

InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR031327 MCM IPR008046 Mcm3 IPR018525 MCM_CS IPR001208 MCM_dom IPR027925 MCM_N IPR033762 MCM_OB IPR012340 NA-bd_OB-fold IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR11630 PTHR11630, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00493 MCM, 1 hit PF14551 MCM_N, 1 hit PF17207 MCM_OB, 1 hit
PRINTSⁱ	PR01657 MCMFAMILY PR01659 MCMPROTEIN3
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit SM00350 MCM, 1 hit
SUPFAMⁱ	SSF50249 SSF50249, 1 hit SSF52540 SSF52540, 2 hits
PROSITEⁱ	View protein in PROSITE PS00847 MCM_1, 1 hit PS50051 MCM_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MCM3_DROME
Accessionⁱ	Q9XYU1Primary (citable) accession number: Q9XYU1 Secondary accession number(s): P91675
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
	Last sequence update:	November 1, 1999
	Last modified:	May 23, 2018
	This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Drosophila annotation project

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9XYU1 (MCM3_DROME)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

DNA replication licensing factor Mcm3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ