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Protein

DNA replication licensing factor Mcm7

Gene

Mcm7

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 388ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-176187 Activation of ATR in response to replication stress
R-DME-68867 Assembly of the pre-replicative complex
R-DME-68949 Orc1 removal from chromatin
R-DME-68962 Activation of the pre-replicative complex
R-DME-69052 Switching of origins to a post-replicative state
R-DME-69300 Removal of licensing factors from origins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor Mcm7 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 7 protein
Short name:
DmMCM3
Gene namesi
Name:Mcm7
ORF Names:CG4978
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0020633 Mcm7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi387K → A: Reduces complex helicase activity. 1 Publication1
Mutagenesisi514R → A: Reduces complex helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004064231 – 720DNA replication licensing factor Mcm7Add BLAST720

Proteomic databases

PaxDbiQ9XYU0
PRIDEiQ9XYU0

Expressioni

Gene expression databases

BgeeiFBgn0020633
ExpressionAtlasiQ9XYU0 differential
GenevisibleiQ9XYU0 DM

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication

Protein-protein interaction databases

BioGridi64419, 13 interactors
DIPiDIP-59081N
IntActiQ9XYU0, 7 interactors
STRINGi7227.FBpp0076312

Structurei

3D structure databases

ProteinModelPortaliQ9XYU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini332 – 538MCMAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi513 – 516Arginine finger4

Phylogenomic databases

eggNOGiKOG0482 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00900000141136
InParanoidiQ9XYU0
KOiK02210
OMAiVKIQEMA
OrthoDBiEOG091G02LB
PhylomeDBiQ9XYU0

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008050 MCM7
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF26 PTHR11630:SF26, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01663 MCMPROTEIN7
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9XYU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRDYAQDR ESIKTFLSEF CKCDDDGKKE FVYGSQLVKL AHREQVLITI
60 70 80 90 100
DLDDLAEFNE SLAEAVVDNC RRYTSIFSDV IAELLPSYKQ QEVHAKDALD
110 120 130 140 150
VYIEHRLMME SRTRNPMEQR DERNSFPSEL MKRFEVGFKP LSTEKAHSIR
160 170 180 190 200
EVKAQHIGKL VTVRGIVTRC TEVKPMMVVA TYTCDRCGSE TYQPVNSLSF
210 220 230 240 250
TPVHDCPSDD CRVNKAGGRL YLQTRGSKFV KFQEVKMQEH SDQVPVGHIP
260 270 280 290 300
RSMTIMCRGE VTRMAQPGDH IVVSGVFLPL MRTGFAQMIQ GLLSETFLQA
310 320 330 340 350
HRIICINKND EISDKDAELT PEELEELAQD DFYERLATSL APEIYGHLDV
360 370 380 390 400
KKALLLLLVG GVDKRPDGMK IRGNINICLM GDPGVAKSQL LGYISRLAVR
410 420 430 440 450
SQYTTGRGSS GVGLTAAVMK DPLTGEMTLE GGALVLADQG VCCIDEFDKM
460 470 480 490 500
ADQDRTAIHE VMEQQTISIA KAGIMTTLNA RVSILAAANP AFGRYNPRRT
510 520 530 540 550
VEQNIQLPAA LLSRFDLLWL IQDKPDRDND LRLAKHITYV HSHSKQPPTR
560 570 580 590 600
VKALDMNLMR RYINLCKRKN PTIPDELTDY IVGAYVELRR EARNQKDMTF
610 620 630 640 650
TSARNLLGIL RLSTALARLR LSDSVEKDDV AEALRLLEMS KDSLNQIHEH
660 670 680 690 700
QKGHVPNTSD RIFAIVRELA GSGKAVKISD IMDRCTTKGF KPDQVDKCID
710 720
DYEELNVWQV NMGRTKITFM
Length:720
Mass (Da):81,284
Last modified:November 1, 1999 - v1
Checksum:i09286B4D5E406756
GO

Sequence cautioni

The sequence BAA34733 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti506Q → E in BAA34733 (PubMed:9795205).Curated1
Sequence conflicti618R → W in BAA34733 (PubMed:9795205).Curated1
Sequence conflicti624S → T in BAA34733 (PubMed:9795205).Curated1
Sequence conflicti659S → T in BAA34733 (PubMed:9795205).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010109 mRNA Translation: BAA34733.1 Sequence problems.
AF124743 mRNA Translation: AAD32857.1
AE014296 Genomic DNA Translation: AAF50357.1
BT001526 mRNA Translation: AAN71281.1
RefSeqiNP_523984.1, NM_079260.3
UniGeneiDm.7221

Genome annotation databases

EnsemblMetazoaiFBtr0076585; FBpp0076312; FBgn0020633
GeneIDi39014
KEGGidme:Dmel_CG4978
UCSCiCG4978-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiMCM7_DROME
AccessioniPrimary (citable) accession number: Q9XYU0
Secondary accession number(s): P91674
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health