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Protein

Superoxide-generating NADPH oxidase heavy chain subunit A

Gene

noxA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior (By similarity).By similarity

Cofactori

FADCurated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron (heme axial ligand)By similarity
Metal bindingi115 – 1151Iron (heme axial ligand)By similarity
Metal bindingi190 – 1901Iron (heme axial ligand)By similarity
Metal bindingi203 – 2031Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2897FADSequence analysis

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: dictyBase
  • heme binding Source: dictyBase
  • metal ion binding Source: UniProtKB-KW
  • superoxide-generating NADPH oxidase activity Source: dictyBase

GO - Biological processi

  • sorocarp development Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • superoxide anion generation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Protein family/group databases

PeroxiBasei6101. DdNOx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide-generating NADPH oxidase heavy chain subunit A (EC:1.-.-.-)
Alternative name(s):
NADPH oxidase A
Superoxide-generating NADPH oxidase flavocytochrome A
Gene namesi
Name:noxA
ORF Names:DDB_G0289653
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289653. noxA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence analysisAdd
BLAST
Transmembranei20 – 4021HelicalSequence analysisAdd
BLAST
Topological domaini41 – 6222ExtracellularSequence analysisAdd
BLAST
Transmembranei63 – 8321HelicalSequence analysisAdd
BLAST
Topological domaini84 – 9714CytoplasmicSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 14931ExtracellularSequence analysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence analysisAdd
BLAST
Topological domaini171 – 18414CytoplasmicSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Topological domaini206 – 2061ExtracellularSequence analysis
Transmembranei207 – 22721HelicalSequence analysisAdd
BLAST
Topological domaini228 – 517290CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: dictyBase
  • NADPH oxidase complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype on vegetative growth, but prevents formation of fruiting bodies.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Superoxide-generating NADPH oxidase heavy chain subunit APRO_0000361527Add
BLAST

Proteomic databases

PaxDbiQ9XYS3.

Expressioni

Developmental stagei

Highly expressed during vegetative stage. Expression decreases during development and is very low in fruiting bodies.1 Publication

Interactioni

Subunit structurei

Composed of a heavy chain and a light chain.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191274.

Structurei

3D structure databases

ProteinModelPortaliQ9XYS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 201144Ferric oxidoreductaseAdd
BLAST
Domaini229 – 349121FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ9XYS3.
KOiK08008.
OMAiTITHKIG.
PhylomeDBiQ9XYS3.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XYS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLPTKEEIQ RYWVNEGNKL ILVILYTLGN IAAFVYTFVH YYNSPAFEVV
60 70 80 90 100
GYGVCFARGC AQLLKLNCAL ILVPVLRNLL SFLRGTFLNN YVPFDKNIVF
110 120 130 140 150
HKLIAWVICF ATFGHVMAHF NNFRLYQDIT PQEYKRILGI DYPNLTPIKY
160 170 180 190 200
AFATLAGWTG HVVCIVMVLM YTSAVESIRR PMFEGFWYTH HLFVVFFGLL
210 220 230 240 250
VVHGLHSILE PTSFWKWVIG PCALYIVERL IRLLRSKKTT MLIQARIHPS
260 270 280 290 300
RVIEVRMKTE RFKYKPGQYL FLNCPTIAQN EWHPFTITSA PEEDFVSCHI
310 320 330 340 350
NVVGNWTGKL STLLNPDKKM GIVQENVLKS PDGKPILRID GPFGAASEEV
360 370 380 390 400
FKYKQVILVG AGIGVTPFAS ILKHIKYQMA RTYNTTPLID KVHFYWICRD
410 420 430 440 450
RNSFEWFSGL IGELEMENHN NFLEIHPYLT GALSAQEIRD VMYGDEEKDL
460 470 480 490 500
ITGFTTPTQF GRPKWDEIFA DHALRYAEKD VGVFFCGPKL LSKSLYKAST
510
HYTKTTTCRF HYNKENF
Length:517
Mass (Da):59,752
Last modified:November 1, 1999 - v1
Checksum:i36A2CD07408F1E46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123275 Genomic DNA. Translation: AAD22057.1.
AAFI02000148 Genomic DNA. Translation: EAL62538.1.
RefSeqiXP_636064.1. XM_630972.1.

Genome annotation databases

EnsemblProtistsiDDB0191274; DDB0191274; DDB_G0289653.
GeneIDi8627274.
KEGGiddi:DDB_G0289653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123275 Genomic DNA. Translation: AAD22057.1.
AAFI02000148 Genomic DNA. Translation: EAL62538.1.
RefSeqiXP_636064.1. XM_630972.1.

3D structure databases

ProteinModelPortaliQ9XYS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191274.

Protein family/group databases

PeroxiBasei6101. DdNOx01.

Proteomic databases

PaxDbiQ9XYS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191274; DDB0191274; DDB_G0289653.
GeneIDi8627274.
KEGGiddi:DDB_G0289653.

Organism-specific databases

dictyBaseiDDB_G0289653. noxA.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ9XYS3.
KOiK08008.
OMAiTITHKIG.
PhylomeDBiQ9XYS3.

Miscellaneous databases

PROiQ9XYS3.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOXA_DICDI
AccessioniPrimary (citable) accession number: Q9XYS3
Secondary accession number(s): Q54H54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: November 1, 1999
Last modified: December 9, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.