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Protein

Superoxide-generating NADPH oxidase heavy chain subunit A

Gene

noxA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior (By similarity).By similarity

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron (heme axial ligand)By similarity1
Metal bindingi115Iron (heme axial ligand)By similarity1
Metal bindingi190Iron (heme axial ligand)By similarity1
Metal bindingi203Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 289FADSequence analysis7

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: dictyBase
  • heme binding Source: dictyBase
  • metal ion binding Source: UniProtKB-KW
  • superoxide-generating NADPH oxidase activity Source: dictyBase

GO - Biological processi

  • defense response to bacterium Source: dictyBase
  • sorocarp development Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • superoxide anion generation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-DDI-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei6101. DdNOx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide-generating NADPH oxidase heavy chain subunit A (EC:1.-.-.-)
Alternative name(s):
NADPH oxidase A
Superoxide-generating NADPH oxidase flavocytochrome A
Gene namesi
Name:noxA
ORF Names:DDB_G0289653
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289653. noxA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicSequence analysisAdd BLAST19
Transmembranei20 – 40HelicalSequence analysisAdd BLAST21
Topological domaini41 – 62ExtracellularSequence analysisAdd BLAST22
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Topological domaini84 – 97CytoplasmicSequence analysisAdd BLAST14
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 149ExtracellularSequence analysisAdd BLAST31
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171 – 184CytoplasmicSequence analysisAdd BLAST14
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206ExtracellularSequence analysis1
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 517CytoplasmicSequence analysisAdd BLAST290

GO - Cellular componenti

  • integral component of membrane Source: dictyBase
  • NADPH oxidase complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype on vegetative growth, but prevents formation of fruiting bodies.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003615271 – 517Superoxide-generating NADPH oxidase heavy chain subunit AAdd BLAST517

Proteomic databases

PaxDbiQ9XYS3.

Expressioni

Developmental stagei

Highly expressed during vegetative stage. Expression decreases during development and is very low in fruiting bodies.1 Publication

Interactioni

Subunit structurei

Composed of a heavy chain and a light chain.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191274.

Structurei

3D structure databases

ProteinModelPortaliQ9XYS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 201Ferric oxidoreductaseAdd BLAST144
Domaini229 – 349FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST121

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ9XYS3.
KOiK08008.
OMAiTITHKIG.
PhylomeDBiQ9XYS3.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XYS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLPTKEEIQ RYWVNEGNKL ILVILYTLGN IAAFVYTFVH YYNSPAFEVV
60 70 80 90 100
GYGVCFARGC AQLLKLNCAL ILVPVLRNLL SFLRGTFLNN YVPFDKNIVF
110 120 130 140 150
HKLIAWVICF ATFGHVMAHF NNFRLYQDIT PQEYKRILGI DYPNLTPIKY
160 170 180 190 200
AFATLAGWTG HVVCIVMVLM YTSAVESIRR PMFEGFWYTH HLFVVFFGLL
210 220 230 240 250
VVHGLHSILE PTSFWKWVIG PCALYIVERL IRLLRSKKTT MLIQARIHPS
260 270 280 290 300
RVIEVRMKTE RFKYKPGQYL FLNCPTIAQN EWHPFTITSA PEEDFVSCHI
310 320 330 340 350
NVVGNWTGKL STLLNPDKKM GIVQENVLKS PDGKPILRID GPFGAASEEV
360 370 380 390 400
FKYKQVILVG AGIGVTPFAS ILKHIKYQMA RTYNTTPLID KVHFYWICRD
410 420 430 440 450
RNSFEWFSGL IGELEMENHN NFLEIHPYLT GALSAQEIRD VMYGDEEKDL
460 470 480 490 500
ITGFTTPTQF GRPKWDEIFA DHALRYAEKD VGVFFCGPKL LSKSLYKAST
510
HYTKTTTCRF HYNKENF
Length:517
Mass (Da):59,752
Last modified:November 1, 1999 - v1
Checksum:i36A2CD07408F1E46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123275 Genomic DNA. Translation: AAD22057.1.
AAFI02000148 Genomic DNA. Translation: EAL62538.1.
RefSeqiXP_636064.1. XM_630972.1.

Genome annotation databases

EnsemblProtistsiEAL62538; EAL62538; DDB_G0289653.
GeneIDi8627274.
KEGGiddi:DDB_G0289653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123275 Genomic DNA. Translation: AAD22057.1.
AAFI02000148 Genomic DNA. Translation: EAL62538.1.
RefSeqiXP_636064.1. XM_630972.1.

3D structure databases

ProteinModelPortaliQ9XYS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191274.

Protein family/group databases

PeroxiBasei6101. DdNOx01.

Proteomic databases

PaxDbiQ9XYS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL62538; EAL62538; DDB_G0289653.
GeneIDi8627274.
KEGGiddi:DDB_G0289653.

Organism-specific databases

dictyBaseiDDB_G0289653. noxA.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ9XYS3.
KOiK08008.
OMAiTITHKIG.
PhylomeDBiQ9XYS3.

Enzyme and pathway databases

ReactomeiR-DDI-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9XYS3.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOXA_DICDI
AccessioniPrimary (citable) accession number: Q9XYS3
Secondary accession number(s): Q54H54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.