Q9XYR5 (CONG_CONGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Contulakin-G Alternative name(s): CGX-1160
Organism	Conus geographus (Geography cone) (Nubecula geographus)
Taxonomic identifier	6491 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as an agonist of neurotensin receptors. It binds to human neurotensin type 1 receptor (NTSR1), rat neurotensin types 1 and 2 receptors (NTSR1/NTSR2) and mouse neurotensin type 3 receptor (SORT1). Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Post-translational modification	O-glycosylated. The glycosylation seems to enhance the affinity to the neurotensin receptors.
Pharmaceutical use	Is under phase II clinical trials under the name CGX-1160 by Cognetix Inc. It is efficacious in a wide range of preclinical pain models (acute, post-surgical, inflammatory, and neuropathic), and is particularly efficacious in models of chronic pain.
Miscellaneous	The mature peptide does not contain cysteine residue.
Sequence similarities	Belongs to the conotoxin C superfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	G-protein coupled receptor impairing toxin Neurotoxin Toxin
PTM	Cleavage on pair of basic residues Glycoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing Pharmaceutical
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 50	28			PRO_0000035083
	Peptide	51 – 66	16	Contulakin-G Ref.1		PRO_0000035084
	Propeptide	67 – 76	10			PRO_0000035085
Amino acid modifications
	Modified residue	51	1	Pyrrolidone carboxylic acid Ref.1
	Glycosylation	60	1	O-linked (GalNAc...)		CAR_000164

Sequences

	Sequence		Length	Mass (Da)
	Q9XYR5 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D8094EB30C6AAFD4	FASTA	76	8,261
	10 20 30 40 50 60 MQTAYWVMVM MMVWIAAPLS EGGKLNDVIR GLVPDDITPQ LILGSLISRR QSEEGGSNAT 70 KKPYILRASD QVASGP

References

[1]

"Contulakin-G, an O-glycosylated invertebrate neurotensin." Craig A.G., Norberg T., Griffin D., Hoeger C., Akhtar M., Schmidt K., Low W., Dykert J., Richelson E., Navarro V., Mazella J., Watkins M., Hillyard D.R., Imperial J., Cruz L.J., Olivera B.M. J. Biol. Chem. 274:13752-13759(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-66, FUNCTION, TOXIN TARGET. Tissue: Venom.

[2]

"Powerful antinociceptive effects of the cone snail venom-derived subtype-selective NMDA receptor antagonists conantokins G and T." Malmberg A.B., Gilbert H., McCabe R.T., Basbaum A.I. Pain 101:109-116(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHARMACEUTICAL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF121108 mRNA. Translation: AAD30031.1.
PIR	A59043.
3D structure databases
ModBase	Search...
PTM databases
GlycoSuiteDB	Q9XYR5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ConoServer	1263. contulakin-G precursor.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

CONG_CONGE

Accession

Primary (citable) accession number: Q9XYR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program