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Protein

Inositol-tetrakisphosphate 1-kinase

Gene

ITPK1

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca2+-activated Cl- channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium.

Catalytic activityi

ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate.1 Publication

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171D-myo-inositol 1,3,4-trisphosphate1
Binding sitei571D-myo-inositol 1,3,4-trisphosphate1
Binding sitei94ATP1
Binding sitei136ATP1
Binding sitei1411D-myo-inositol 1,3,4-trisphosphate1
Binding sitei1471D-myo-inositol 1,3,4-trisphosphate1
Binding sitei1791D-myo-inositol 1,3,4-trisphosphate1
Binding sitei194ATP1
Binding sitei210ATP1
Metal bindingi275Magnesium 11
Metal bindingi289Magnesium 11
Metal bindingi289Magnesium 21
Metal bindingi291Magnesium 21
Binding sitei2911D-myo-inositol 1,3,4-trisphosphate1
Binding sitei2951D-myo-inositol 1,3,4-trisphosphate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 179ATPAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.159. 2080.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-tetrakisphosphate 1-kinase (EC:2.7.1.134)
Alternative name(s):
Inositol 1,3,4-trisphosphate 5/6-kinase (EC:2.7.1.159)
Short name:
Inositol-triphosphate 5/6-kinase
Short name:
Ins(1,3,4)P(3) 5/6-kinase
Gene namesi
Name:ITPK1
ORF Names:151.t00008
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
Proteomesi
  • UP000001926 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiAmoebaDB:EHI_100310.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208381 – 319Inositol-tetrakisphosphate 1-kinaseAdd BLAST319

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi5759.rna_EHI_100310-1.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi15 – 21Combined sources7
Beta strandi22 – 24Combined sources3
Beta strandi27 – 33Combined sources7
Beta strandi36 – 45Combined sources10
Beta strandi52 – 56Combined sources5
Beta strandi61 – 63Combined sources3
Helixi64 – 75Combined sources12
Beta strandi79 – 82Combined sources4
Helixi85 – 91Combined sources7
Helixi94 – 103Combined sources10
Beta strandi111 – 116Combined sources6
Helixi117 – 125Combined sources9
Beta strandi131 – 139Combined sources9
Beta strandi141 – 144Combined sources4
Helixi145 – 147Combined sources3
Beta strandi148 – 152Combined sources5
Helixi155 – 158Combined sources4
Beta strandi163 – 169Combined sources7
Beta strandi177 – 183Combined sources7
Beta strandi186 – 192Combined sources7
Beta strandi205 – 209Combined sources5
Helixi213 – 218Combined sources6
Helixi226 – 233Combined sources8
Turni234 – 237Combined sources4
Turni245 – 249Combined sources5
Helixi253 – 267Combined sources15
Beta strandi270 – 277Combined sources8
Helixi279 – 281Combined sources3
Beta strandi286 – 293Combined sources8
Beta strandi298 – 300Combined sources3
Helixi302 – 314Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z2NX-ray1.20X1-319[»]
1Z2OX-ray1.24X1-319[»]
1Z2PX-ray1.22X1-319[»]
ProteinModelPortaliQ9XYQ1.
SMRiQ9XYQ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9XYQ1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 317ATP-graspPROSITE-ProRule annotationAdd BLAST220

Sequence similaritiesi

Belongs to the ITPK1 family.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHA6. Eukaryota.
ENOG4110KIK. LUCA.
InParanoidiQ9XYQ1.
KOiK00913.
OMAiENSANRF.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR008656. Inositol_tetrakis-P_1-kinase.
[Graphical view]
PfamiPF05770. Ins134_P3_kin. 1 hit.
[Graphical view]
PIRSFiPIRSF038186. ITPK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XYQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKQTVSLF IWLPESKQKT LFISTKNHTQ FELNNIIFDV TLSTELPDKE
60 70 80 90 100
PNAIITKRTH PVGKMADEMR KYEKDHPKVL FLESSAIHDM MSSREEINAL
110 120 130 140 150
LIKNNIPIPN SFSVKSKEEV IQLLQSKQLI LPFIVKPENA QGTFNAHQMK
160 170 180 190 200
IVLEQEGIDD IHFPCLCQHY INHNNKIVKV FCIGNTLKWQ TRTSLPNVHR
210 220 230 240 250
CGIKSVDFNN QHLEDILSWP EGVIDKQDII ENSANRFGSK ILEDPILLNL
260 270 280 290 300
TSEAEMRDLA YKVRCALGVQ LCGIDFIKEN EQGNPLVVDV NVFPSYGGKV
310
DFDWFVEKVA LCYTEVAKI
Length:319
Mass (Da):36,480
Last modified:November 1, 1999 - v1
Checksum:iD526DBF2E897305D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118848 Genomic DNA. Translation: AAD22969.1.
DS571201 Genomic DNA. Translation: EAL46318.1.
RefSeqiXP_651704.1. XM_646612.1.

Genome annotation databases

GeneIDi3406022.
KEGGiehi:EHI_100310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118848 Genomic DNA. Translation: AAD22969.1.
DS571201 Genomic DNA. Translation: EAL46318.1.
RefSeqiXP_651704.1. XM_646612.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z2NX-ray1.20X1-319[»]
1Z2OX-ray1.24X1-319[»]
1Z2PX-ray1.22X1-319[»]
ProteinModelPortaliQ9XYQ1.
SMRiQ9XYQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5759.rna_EHI_100310-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3406022.
KEGGiehi:EHI_100310.

Organism-specific databases

EuPathDBiAmoebaDB:EHI_100310.

Phylogenomic databases

eggNOGiENOG410IHA6. Eukaryota.
ENOG4110KIK. LUCA.
InParanoidiQ9XYQ1.
KOiK00913.
OMAiENSANRF.

Enzyme and pathway databases

BRENDAi2.7.1.159. 2080.

Miscellaneous databases

EvolutionaryTraceiQ9XYQ1.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR008656. Inositol_tetrakis-P_1-kinase.
[Graphical view]
PfamiPF05770. Ins134_P3_kin. 1 hit.
[Graphical view]
PIRSFiPIRSF038186. ITPK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITPK1_ENTHI
AccessioniPrimary (citable) accession number: Q9XYQ1
Secondary accession number(s): Q50XK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.