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Protein

Inositol-tetrakisphosphate 1-kinase

Gene

ITPK1

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca2+-activated Cl- channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium.

Catalytic activityi

ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate.1 Publication

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 1711D-myo-inositol 1,3,4-trisphosphate
Binding sitei57 – 5711D-myo-inositol 1,3,4-trisphosphate
Binding sitei94 – 941ATP
Binding sitei136 – 1361ATP
Binding sitei141 – 14111D-myo-inositol 1,3,4-trisphosphate
Binding sitei147 – 14711D-myo-inositol 1,3,4-trisphosphate
Binding sitei179 – 17911D-myo-inositol 1,3,4-trisphosphate
Binding sitei194 – 1941ATP
Binding sitei210 – 2101ATP
Metal bindingi275 – 2751Magnesium 1
Metal bindingi289 – 2891Magnesium 1
Metal bindingi289 – 2891Magnesium 2
Metal bindingi291 – 2911Magnesium 2
Binding sitei291 – 29111D-myo-inositol 1,3,4-trisphosphate
Binding sitei295 – 29511D-myo-inositol 1,3,4-trisphosphate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi168 – 17912ATPAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.159. 2080.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-tetrakisphosphate 1-kinase (EC:2.7.1.134)
Alternative name(s):
Inositol 1,3,4-trisphosphate 5/6-kinase (EC:2.7.1.159)
Short name:
Inositol-triphosphate 5/6-kinase
Short name:
Ins(1,3,4)P(3) 5/6-kinase
Gene namesi
Name:ITPK1
ORF Names:151.t00008
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
Proteomesi
  • UP000001926 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiAmoebaDB:EHI_100310.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Inositol-tetrakisphosphate 1-kinasePRO_0000220838Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi5759.rna_EHI_100310-1.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi15 – 217Combined sources
Beta strandi22 – 243Combined sources
Beta strandi27 – 337Combined sources
Beta strandi36 – 4510Combined sources
Beta strandi52 – 565Combined sources
Beta strandi61 – 633Combined sources
Helixi64 – 7512Combined sources
Beta strandi79 – 824Combined sources
Helixi85 – 917Combined sources
Helixi94 – 10310Combined sources
Beta strandi111 – 1166Combined sources
Helixi117 – 1259Combined sources
Beta strandi131 – 1399Combined sources
Beta strandi141 – 1444Combined sources
Helixi145 – 1473Combined sources
Beta strandi148 – 1525Combined sources
Helixi155 – 1584Combined sources
Beta strandi163 – 1697Combined sources
Beta strandi177 – 1837Combined sources
Beta strandi186 – 1927Combined sources
Beta strandi205 – 2095Combined sources
Helixi213 – 2186Combined sources
Helixi226 – 2338Combined sources
Turni234 – 2374Combined sources
Turni245 – 2495Combined sources
Helixi253 – 26715Combined sources
Beta strandi270 – 2778Combined sources
Helixi279 – 2813Combined sources
Beta strandi286 – 2938Combined sources
Beta strandi298 – 3003Combined sources
Helixi302 – 31413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z2NX-ray1.20X1-319[»]
1Z2OX-ray1.24X1-319[»]
1Z2PX-ray1.22X1-319[»]
ProteinModelPortaliQ9XYQ1.
SMRiQ9XYQ1. Positions 5-315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9XYQ1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 317220ATP-graspPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ITPK1 family.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHA6. Eukaryota.
ENOG4110KIK. LUCA.
InParanoidiQ9XYQ1.
KOiK00913.
OMAiENSANRF.

Family and domain databases

InterProiIPR011761. ATP-grasp.
IPR008656. Inositol_tetrakis-P_1-kinase.
[Graphical view]
PfamiPF05770. Ins134_P3_kin. 1 hit.
[Graphical view]
PIRSFiPIRSF038186. ITPK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XYQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKQTVSLF IWLPESKQKT LFISTKNHTQ FELNNIIFDV TLSTELPDKE
60 70 80 90 100
PNAIITKRTH PVGKMADEMR KYEKDHPKVL FLESSAIHDM MSSREEINAL
110 120 130 140 150
LIKNNIPIPN SFSVKSKEEV IQLLQSKQLI LPFIVKPENA QGTFNAHQMK
160 170 180 190 200
IVLEQEGIDD IHFPCLCQHY INHNNKIVKV FCIGNTLKWQ TRTSLPNVHR
210 220 230 240 250
CGIKSVDFNN QHLEDILSWP EGVIDKQDII ENSANRFGSK ILEDPILLNL
260 270 280 290 300
TSEAEMRDLA YKVRCALGVQ LCGIDFIKEN EQGNPLVVDV NVFPSYGGKV
310
DFDWFVEKVA LCYTEVAKI
Length:319
Mass (Da):36,480
Last modified:November 1, 1999 - v1
Checksum:iD526DBF2E897305D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118848 Genomic DNA. Translation: AAD22969.1.
DS571201 Genomic DNA. Translation: EAL46318.1.
RefSeqiXP_651704.1. XM_646612.1.

Genome annotation databases

GeneIDi3406022.
KEGGiehi:EHI_100310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118848 Genomic DNA. Translation: AAD22969.1.
DS571201 Genomic DNA. Translation: EAL46318.1.
RefSeqiXP_651704.1. XM_646612.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z2NX-ray1.20X1-319[»]
1Z2OX-ray1.24X1-319[»]
1Z2PX-ray1.22X1-319[»]
ProteinModelPortaliQ9XYQ1.
SMRiQ9XYQ1. Positions 5-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5759.rna_EHI_100310-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3406022.
KEGGiehi:EHI_100310.

Organism-specific databases

EuPathDBiAmoebaDB:EHI_100310.

Phylogenomic databases

eggNOGiENOG410IHA6. Eukaryota.
ENOG4110KIK. LUCA.
InParanoidiQ9XYQ1.
KOiK00913.
OMAiENSANRF.

Enzyme and pathway databases

BRENDAi2.7.1.159. 2080.

Miscellaneous databases

EvolutionaryTraceiQ9XYQ1.

Family and domain databases

InterProiIPR011761. ATP-grasp.
IPR008656. Inositol_tetrakis-P_1-kinase.
[Graphical view]
PfamiPF05770. Ins134_P3_kin. 1 hit.
[Graphical view]
PIRSFiPIRSF038186. ITPK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITPK1_ENTHI
AccessioniPrimary (citable) accession number: Q9XYQ1
Secondary accession number(s): Q50XK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.