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Protein

Caspase Dronc

Gene

Dronc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with Diap1 is required to suppress Dronc-mediated cell death; via Diap1-mediated ubiquitination of Dronc. Rate-limiting caspase in rpr and hid death pathway.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei271By similarity1
Active sitei318By similarity1

GO - Molecular functioni

  • CARD domain binding Source: FlyBase
  • cysteine-type endopeptidase activity Source: FlyBase
  • cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: FlyBase
  • endopeptidase activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
  • apoptotic process Source: FlyBase
  • autophagic cell death Source: FlyBase
  • central nervous system development Source: FlyBase
  • compound eye development Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • ectopic germ cell programmed cell death Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • head involution Source: FlyBase
  • hemocyte development Source: FlyBase
  • melanization defense response Source: FlyBase
  • metamorphosis Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • neuron remodeling Source: FlyBase
  • nurse cell apoptotic process Source: FlyBase
  • positive regulation of compound eye retinal cell programmed cell death Source: FlyBase
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: FlyBase
  • programmed cell death Source: FlyBase
  • programmed cell death involved in cell development Source: FlyBase
  • protein autoprocessing Source: FlyBase
  • protein heterooligomerization Source: FlyBase
  • regulation of autophagy Source: FlyBase
  • regulation of cell death Source: FlyBase
  • regulation of retinal cell programmed cell death Source: FlyBase
  • salivary gland cell autophagic cell death Source: FlyBase
  • salivary gland histolysis Source: FlyBase
  • sensory organ development Source: FlyBase
  • sperm individualization Source: FlyBase
  • zymogen activation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-DME-198323. AKT phosphorylates targets in the cytosol.
R-DME-448706. Interleukin-1 processing.

Protein family/group databases

MEROPSiC14.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase Dronc (EC:3.4.22.-)
Alternative name(s):
NEDD2-like caspase
Cleaved into the following 2 chains:
Gene namesi
Name:DroncImported
Synonyms:NcImported
ORF Names:CG8091Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0026404. Dronc.

Subcellular locationi

GO - Cellular componenti

  • apoptosome Source: FlyBase
  • cytoplasm Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118F → E: Disrupts interaction with Diap1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000046701 – 1341 PublicationAdd BLAST134
ChainiPRO_0000004671135 – 320Caspase Nc subunit 1Add BLAST186
PropeptideiPRO_0000004672321 – 3241 Publication4
ChainiPRO_0000004673325 – 450Caspase Nc subunit 2Add BLAST126

Post-translational modificationi

Ubiquitinated by Diap1, leading to its subsequent degradation.1 Publication

Keywords - PTMi

Ubl conjugation, Zymogen

Proteomic databases

PaxDbiQ9XYF4.
PRIDEiQ9XYF4.

Expressioni

Tissue specificityi

Ubiquitously expressed in embryos during early stages of development. In late third instar larvae, dramatic up-regulation in salivary glands and midgut before histolysis of these tissues.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Inductioni

By ecdysone; exposure of salivary glands and midgut isolated from second instar larvae results in a massive increase in levels.1 Publication

Gene expression databases

BgeeiFBgn0026404.
GenevisibleiQ9XYF4. DM.

Interactioni

Subunit structurei

Interacts with Diap1; residues 114-125 interact with the second BIR domain of Diap1. Can form a stable complex with Drice. Rpr can out-compete Dronc for binding Diap1, therefore removing Diap1-mediated ubiquitination.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Diap1Q243069EBI-108311,EBI-456419
DriceO013823EBI-108311,EBI-91422

GO - Molecular functioni

  • CARD domain binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi64560. 19 interactors.
DIPiDIP-31484N.
IntActiQ9XYF4. 4 interactors.
MINTiMINT-8048660.
STRINGi7227.FBpp0076076.

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi195 – 202Combined sources8
Helixi216 – 229Combined sources14
Beta strandi232 – 237Combined sources6
Helixi242 – 253Combined sources12
Helixi256 – 260Combined sources5
Beta strandi264 – 270Combined sources7
Beta strandi280 – 282Combined sources3
Beta strandi288 – 290Combined sources3
Helixi291 – 296Combined sources6
Turni300 – 302Combined sources3
Helixi304 – 306Combined sources3
Beta strandi311 – 316Combined sources6
Beta strandi366 – 371Combined sources6
Turni380 – 382Combined sources3
Helixi385 – 400Combined sources16
Turni401 – 403Combined sources3
Helixi406 – 418Combined sources13
Beta strandi419 – 423Combined sources5
Beta strandi425 – 427Combined sources3
Beta strandi431 – 436Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q4QX-ray2.10K/L/M/N/O/P/Q/R/S/T114-125[»]
2FP3X-ray2.50A136-450[»]
3J9Kelectron microscopy4.10B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f1-450[»]
ProteinModelPortaliQ9XYF4.
SMRiQ9XYF4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9XYF4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 109CARDSequence analysisAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni114 – 125Required for binding thAdd BLAST12

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated
Contains 1 CARD domain.Sequence analysis

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
InParanoidiQ9XYF4.
KOiK20009.
OMAiVMVLMTH.
OrthoDBiEOG091G0XCA.
PhylomeDBiQ9XYF4.

Family and domain databases

CDDicd00032. CASc. 1 hit.
Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR011029. DEATH-like_dom.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
IPR017350. Pept_C14A_CASP1-typ.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XYF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPPELEIGM PKRHREHIRK NLNILVEWTN YERLAMECVQ QGILTVQMLR
60 70 80 90 100
NTQDLNGKPF NMDEKDVRVE QHRRLLLKIT QRGPTAYNLL INALRNINCL
110 120 130 140 150
DAAVLLESVD ESDSRPPFIS LNERRTSRKS ADIVDTPSPE ASEGPCVSKL
160 170 180 190 200
RNEPLGALTP YVGVVDGPEV KKSKKIHGGD SAILGTYKMQ SRFNRGVLLM
210 220 230 240 250
VNIMDYPDQN RRRIGAEKDS KSLIHLFQEL NFTIFPYGNV NQDQFFKLLT
260 270 280 290 300
MVTSSSYVQN TECFVMVLMT HGNSVEGKEK VEFCDGSVVD MQKIKDHFQT
310 320 330 340 350
AKCPYLVNKP KVLMFPFCRG DEYDLGHPKN QGNLMEPVYT AQEEKWPDTQ
360 370 380 390 400
TEGIPSPSTN VPSLADTLVC YANTPGYVTH RDLDTGSWYI QKFCQVMADH
410 420 430 440 450
AHDTDLEDIL KKTSEAVGNK RTKKGSMQTG AYDNLGFNKK LYFNPGFFNE
Length:450
Mass (Da):51,141
Last modified:November 1, 1999 - v1
Checksum:i640A9725202A7E65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104357 mRNA. Translation: AAD26625.1.
AJ242796 mRNA. Translation: CAB53565.1.
AE014296 Genomic DNA. Translation: AAF50180.1.
AY058747 mRNA. Translation: AAL13976.1.
RefSeqiNP_524017.1. NM_079293.4.
UniGeneiDm.3230.

Genome annotation databases

EnsemblMetazoaiFBtr0076347; FBpp0076076; FBgn0026404.
GeneIDi39173.
KEGGidme:Dmel_CG8091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104357 mRNA. Translation: AAD26625.1.
AJ242796 mRNA. Translation: CAB53565.1.
AE014296 Genomic DNA. Translation: AAF50180.1.
AY058747 mRNA. Translation: AAL13976.1.
RefSeqiNP_524017.1. NM_079293.4.
UniGeneiDm.3230.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q4QX-ray2.10K/L/M/N/O/P/Q/R/S/T114-125[»]
2FP3X-ray2.50A136-450[»]
3J9Kelectron microscopy4.10B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f1-450[»]
ProteinModelPortaliQ9XYF4.
SMRiQ9XYF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64560. 19 interactors.
DIPiDIP-31484N.
IntActiQ9XYF4. 4 interactors.
MINTiMINT-8048660.
STRINGi7227.FBpp0076076.

Protein family/group databases

MEROPSiC14.019.

Proteomic databases

PaxDbiQ9XYF4.
PRIDEiQ9XYF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076347; FBpp0076076; FBgn0026404.
GeneIDi39173.
KEGGidme:Dmel_CG8091.

Organism-specific databases

CTDi39173.
FlyBaseiFBgn0026404. Dronc.

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
InParanoidiQ9XYF4.
KOiK20009.
OMAiVMVLMTH.
OrthoDBiEOG091G0XCA.
PhylomeDBiQ9XYF4.

Enzyme and pathway databases

ReactomeiR-DME-198323. AKT phosphorylates targets in the cytosol.
R-DME-448706. Interleukin-1 processing.

Miscellaneous databases

EvolutionaryTraceiQ9XYF4.
GenomeRNAii39173.
PROiQ9XYF4.

Gene expression databases

BgeeiFBgn0026404.
GenevisibleiQ9XYF4. DM.

Family and domain databases

CDDicd00032. CASc. 1 hit.
Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR011029. DEATH-like_dom.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
IPR017350. Pept_C14A_CASP1-typ.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDRONC_DROME
AccessioniPrimary (citable) accession number: Q9XYF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

The promiscuous caspase inhibitor p35 is neither cleaved by Dronc in vitro nor blocks Dronc activity in vivo.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.