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Protein

Caspase Dronc

Gene

Dronc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with Diap1 is required to suppress Dronc-mediated cell death; via Diap1-mediated ubiquitination of Dronc. Rate-limiting caspase in rpr and hid death pathway.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei271 – 2711By similarity
Active sitei318 – 3181By similarity

GO - Molecular functioni

  • CARD domain binding Source: FlyBase
  • cysteine-type endopeptidase activity Source: FlyBase
  • cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: FlyBase
  • endopeptidase activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
  • apoptotic process Source: FlyBase
  • autophagic cell death Source: FlyBase
  • central nervous system development Source: FlyBase
  • compound eye development Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • ectopic germ cell programmed cell death Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • head involution Source: FlyBase
  • hemocyte development Source: FlyBase
  • melanization defense response Source: FlyBase
  • metamorphosis Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • neuron remodeling Source: FlyBase
  • nurse cell apoptotic process Source: FlyBase
  • positive regulation of compound eye retinal cell programmed cell death Source: FlyBase
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: FlyBase
  • programmed cell death Source: FlyBase
  • programmed cell death involved in cell development Source: FlyBase
  • protein autoprocessing Source: FlyBase
  • protein heterooligomerization Source: FlyBase
  • regulation of autophagy Source: FlyBase
  • regulation of cell death Source: FlyBase
  • regulation of retinal cell programmed cell death Source: FlyBase
  • salivary gland cell autophagic cell death Source: FlyBase
  • salivary gland histolysis Source: FlyBase
  • sensory organ development Source: FlyBase
  • sperm individualization Source: FlyBase
  • zymogen activation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-DME-111458. Formation of apoptosome.
R-DME-111459. Activation of caspases through apoptosome-mediated cleavage.
R-DME-198323. AKT phosphorylates targets in the cytosol.
R-DME-448706. Interleukin-1 processing.

Protein family/group databases

MEROPSiC14.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase Dronc (EC:3.4.22.-)
Alternative name(s):
NEDD2-like caspase
Cleaved into the following 2 chains:
Gene namesi
Name:DroncImported
Synonyms:NcImported
ORF Names:CG8091Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0026404. Dronc.

Subcellular locationi

GO - Cellular componenti

  • apoptosome Source: FlyBase
  • cytoplasm Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181F → E: Disrupts interaction with Diap1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1341341 PublicationPRO_0000004670Add
BLAST
Chaini135 – 320186Caspase Nc subunit 1PRO_0000004671Add
BLAST
Propeptidei321 – 32441 PublicationPRO_0000004672
Chaini325 – 450126Caspase Nc subunit 2PRO_0000004673Add
BLAST

Post-translational modificationi

Ubiquitinated by Diap1, leading to its subsequent degradation.1 Publication

Keywords - PTMi

Ubl conjugation, Zymogen

Proteomic databases

PaxDbiQ9XYF4.
PRIDEiQ9XYF4.

Expressioni

Tissue specificityi

Ubiquitously expressed in embryos during early stages of development. In late third instar larvae, dramatic up-regulation in salivary glands and midgut before histolysis of these tissues.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Inductioni

By ecdysone; exposure of salivary glands and midgut isolated from second instar larvae results in a massive increase in levels.1 Publication

Gene expression databases

BgeeiFBgn0026404.
GenevisibleiQ9XYF4. DM.

Interactioni

Subunit structurei

Interacts with Diap1; residues 114-125 interact with the second BIR domain of Diap1. Can form a stable complex with Drice. Rpr can out-compete Dronc for binding Diap1, therefore removing Diap1-mediated ubiquitination.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Diap1Q243069EBI-108311,EBI-456419
DriceO013823EBI-108311,EBI-91422

GO - Molecular functioni

  • CARD domain binding Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi64560. 19 interactions.
DIPiDIP-31484N.
IntActiQ9XYF4. 4 interactions.
MINTiMINT-8048660.
STRINGi7227.FBpp0076076.

Structurei

Secondary structure

1
450
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi195 – 2028Combined sources
Helixi216 – 22914Combined sources
Beta strandi232 – 2376Combined sources
Helixi242 – 25312Combined sources
Helixi256 – 2605Combined sources
Beta strandi264 – 2707Combined sources
Beta strandi280 – 2823Combined sources
Beta strandi288 – 2903Combined sources
Helixi291 – 2966Combined sources
Turni300 – 3023Combined sources
Helixi304 – 3063Combined sources
Beta strandi311 – 3166Combined sources
Beta strandi366 – 3716Combined sources
Turni380 – 3823Combined sources
Helixi385 – 40016Combined sources
Turni401 – 4033Combined sources
Helixi406 – 41813Combined sources
Beta strandi419 – 4235Combined sources
Beta strandi425 – 4273Combined sources
Beta strandi431 – 4366Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q4QX-ray2.10K/L/M/N/O/P/Q/R/S/T114-125[»]
2FP3X-ray2.50A136-450[»]
3J9Kelectron microscopy4.10B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f1-450[»]
ProteinModelPortaliQ9XYF4.
SMRiQ9XYF4. Positions 157-449.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9XYF4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 10946CARDSequence analysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 12512Required for binding thAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated
Contains 1 CARD domain.Sequence analysis

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
InParanoidiQ9XYF4.
KOiK20009.
OMAiVMVLMTH.
OrthoDBiEOG091G0XCA.
PhylomeDBiQ9XYF4.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR011029. DEATH-like_dom.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
IPR017350. Pept_C14A_CASP1-typ.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XYF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPPELEIGM PKRHREHIRK NLNILVEWTN YERLAMECVQ QGILTVQMLR
60 70 80 90 100
NTQDLNGKPF NMDEKDVRVE QHRRLLLKIT QRGPTAYNLL INALRNINCL
110 120 130 140 150
DAAVLLESVD ESDSRPPFIS LNERRTSRKS ADIVDTPSPE ASEGPCVSKL
160 170 180 190 200
RNEPLGALTP YVGVVDGPEV KKSKKIHGGD SAILGTYKMQ SRFNRGVLLM
210 220 230 240 250
VNIMDYPDQN RRRIGAEKDS KSLIHLFQEL NFTIFPYGNV NQDQFFKLLT
260 270 280 290 300
MVTSSSYVQN TECFVMVLMT HGNSVEGKEK VEFCDGSVVD MQKIKDHFQT
310 320 330 340 350
AKCPYLVNKP KVLMFPFCRG DEYDLGHPKN QGNLMEPVYT AQEEKWPDTQ
360 370 380 390 400
TEGIPSPSTN VPSLADTLVC YANTPGYVTH RDLDTGSWYI QKFCQVMADH
410 420 430 440 450
AHDTDLEDIL KKTSEAVGNK RTKKGSMQTG AYDNLGFNKK LYFNPGFFNE
Length:450
Mass (Da):51,141
Last modified:November 1, 1999 - v1
Checksum:i640A9725202A7E65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104357 mRNA. Translation: AAD26625.1.
AJ242796 mRNA. Translation: CAB53565.1.
AE014296 Genomic DNA. Translation: AAF50180.1.
AY058747 mRNA. Translation: AAL13976.1.
RefSeqiNP_524017.1. NM_079293.4.
UniGeneiDm.3230.

Genome annotation databases

EnsemblMetazoaiFBtr0076347; FBpp0076076; FBgn0026404.
GeneIDi39173.
KEGGidme:Dmel_CG8091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104357 mRNA. Translation: AAD26625.1.
AJ242796 mRNA. Translation: CAB53565.1.
AE014296 Genomic DNA. Translation: AAF50180.1.
AY058747 mRNA. Translation: AAL13976.1.
RefSeqiNP_524017.1. NM_079293.4.
UniGeneiDm.3230.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q4QX-ray2.10K/L/M/N/O/P/Q/R/S/T114-125[»]
2FP3X-ray2.50A136-450[»]
3J9Kelectron microscopy4.10B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f1-450[»]
ProteinModelPortaliQ9XYF4.
SMRiQ9XYF4. Positions 157-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64560. 19 interactions.
DIPiDIP-31484N.
IntActiQ9XYF4. 4 interactions.
MINTiMINT-8048660.
STRINGi7227.FBpp0076076.

Protein family/group databases

MEROPSiC14.019.

Proteomic databases

PaxDbiQ9XYF4.
PRIDEiQ9XYF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076347; FBpp0076076; FBgn0026404.
GeneIDi39173.
KEGGidme:Dmel_CG8091.

Organism-specific databases

CTDi39173.
FlyBaseiFBgn0026404. Dronc.

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
InParanoidiQ9XYF4.
KOiK20009.
OMAiVMVLMTH.
OrthoDBiEOG091G0XCA.
PhylomeDBiQ9XYF4.

Enzyme and pathway databases

ReactomeiR-DME-111458. Formation of apoptosome.
R-DME-111459. Activation of caspases through apoptosome-mediated cleavage.
R-DME-198323. AKT phosphorylates targets in the cytosol.
R-DME-448706. Interleukin-1 processing.

Miscellaneous databases

EvolutionaryTraceiQ9XYF4.
GenomeRNAii39173.
PROiQ9XYF4.

Gene expression databases

BgeeiFBgn0026404.
GenevisibleiQ9XYF4. DM.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR011029. DEATH-like_dom.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
IPR017350. Pept_C14A_CASP1-typ.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDRONC_DROME
AccessioniPrimary (citable) accession number: Q9XYF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

The promiscuous caspase inhibitor p35 is neither cleaved by Dronc in vitro nor blocks Dronc activity in vivo.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.