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Protein

ATP synthase subunit alpha, mitochondrial

Gene

H28O16.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei398 – 3981Required for activitySequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2048ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit alpha, mitochondrial
Gene namesi
ORF Names:H28O16.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiH28O16.1a; CE18826; WBGene00010419.
H28O16.1b; CE34194; WBGene00010419.
H28O16.1c; CE34195; WBGene00010419.
H28O16.1d; CE36263; WBGene00010419.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 538ATP synthase subunit alpha, mitochondrialPRO_0000239935
Transit peptidei1 – ?MitochondrionSequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9XXK1.
PaxDbiQ9XXK1.
PRIDEiQ9XXK1.

2D gel databases

World-2DPAGE0020:Q9XXK1.

PTM databases

iPTMnetiQ9XXK1.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.Curated

Protein-protein interaction databases

BioGridi38531. 3 interactions.
DIPiDIP-25446N.
IntActiQ9XXK1. 2 interactions.
MINTiMINT-213652.
STRINGi6239.H28O16.1a.

Structurei

3D structure databases

ProteinModelPortaliQ9XXK1.
SMRiQ9XXK1. Positions 51-529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Sequence analysis

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1353. Eukaryota.
COG0056. LUCA.
HOGENOMiHOG000130111.
InParanoidiQ9XXK1.
KOiK02132.
OMAiQPMSISE.
PhylomeDBiQ9XXK1.

Family and domain databases

Gene3Di2.40.30.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01346. ATP_synth_alpha_bact.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR023366. ATPase_asu-like.
IPR005294. ATPase_F1-cplx_asu.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR15184:SF3. PTHR15184:SF3. 1 hit.
PfamiPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039088. F_ATPase_subunit_alpha. 1 hit.
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00962. atpA. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: Q9XXK1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSKRIVTAL NTAVKVQNAG IATTARGMAG ASGSEVSKIL EERILGTETG
60 70 80 90 100
INLEETGKVL SIGDGIARVY GLKNIQAEEM VEFDSGIKGM AMNLDVDNVG
110 120 130 140 150
VVVFGNDKVI REGDIVKRTG AIVDVPVGDG LLGRVVDALG NPIDGKGPIA
160 170 180 190 200
NARRSRVEVK APGIIPRLSV REPMVTGVKA VDSLVPIGRG QRELIIGDRQ
210 220 230 240 250
TGKTAIAIDT IINQKRFNDA GDDKKKLFCI YVAVGQKRST VAQIVKRLTD
260 270 280 290 300
AGAMDYTIVV SATASDAAPL QFLAPYSGCA MGEHFRDNGK HALIIFDDLS
310 320 330 340 350
KQAVAYRQMS LLLRRPPGRE AYPGDVFYLH SRLLERAAKM NNSLGGGSLT
360 370 380 390 400
ALPVIETQAG DVSAYIPTNV ISITDGQIFL ETELFYKGVR PAINVGLSVS
410 420 430 440 450
RVGSAAQTKA MKQVAGSMKL ELAQYREVAA FAQFGSDLDA STQQLLNRGV
460 470 480 490 500
RLTELLKQGQ YVPMGIEEQV GVIYAGVKGY LDKVDPSAIT KFEKEFLAHL
510 520 530
RSSQQALLKT IREEGQISPQ TDAQLKDVVV NFLATFKP
Note: No experimental confirmation available.Curated
Length:538
Mass (Da):57,788
Last modified:November 1, 1999 - v1
Checksum:iCC0358C33A4D66D2
GO
Isoform b1 Publication (identifier: Q9XXK1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-146: VIREGDIVKR...DALGNPIDGK → ISCRSQGPRN...FSCANWTWTT
     147-538: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:146
Mass (Da):15,715
Checksum:i6643B1A839996967
GO
Isoform c1 Publication (identifier: Q9XXK1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-138: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:503
Mass (Da):54,154
Checksum:iCDA695624BDD81EB
GO
Isoform d1 Publication (identifier: Q9XXK1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:511
Mass (Da):55,020
Checksum:iB37181C9E8947140
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2727Missing in isoform d. 1 PublicationVSP_052061Add
BLAST
Alternative sequencei104 – 13835Missing in isoform c. 1 PublicationVSP_052062Add
BLAST
Alternative sequencei109 – 14638VIREG…PIDGK → ISCRSQGPRNHPTFVCARTN GYWSQGRRFSCANWTWTT in isoform b. 1 PublicationVSP_052063Add
BLAST
Alternative sequencei147 – 538392Missing in isoform b. 1 PublicationVSP_052064Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023815 Genomic DNA. Translation: CAA19429.1.
AL023815 Genomic DNA. Translation: CAD92383.1.
AL023815 Genomic DNA. Translation: CAD92384.1.
AL023815 Genomic DNA. Translation: CAF31480.1.
PIRiT23128.
RefSeqiNP_001021526.1. NM_001026355.3. [Q9XXK1-1]
NP_001021527.1. NM_001026356.2. [Q9XXK1-2]
NP_001021528.1. NM_001026357.6. [Q9XXK1-3]
NP_001021529.1. NM_001026358.4. [Q9XXK1-4]
UniGeneiCel.22874.

Genome annotation databases

EnsemblMetazoaiH28O16.1a.1; H28O16.1a.1; WBGene00010419. [Q9XXK1-1]
H28O16.1a.2; H28O16.1a.2; WBGene00010419. [Q9XXK1-1]
H28O16.1a.3; H28O16.1a.3; WBGene00010419. [Q9XXK1-1]
H28O16.1a.4; H28O16.1a.4; WBGene00010419. [Q9XXK1-1]
H28O16.1a.5; H28O16.1a.5; WBGene00010419. [Q9XXK1-1]
GeneIDi173134.
KEGGicel:CELE_H28O16.1.
UCSCiH28O16.1a. c. elegans. [Q9XXK1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023815 Genomic DNA. Translation: CAA19429.1.
AL023815 Genomic DNA. Translation: CAD92383.1.
AL023815 Genomic DNA. Translation: CAD92384.1.
AL023815 Genomic DNA. Translation: CAF31480.1.
PIRiT23128.
RefSeqiNP_001021526.1. NM_001026355.3. [Q9XXK1-1]
NP_001021527.1. NM_001026356.2. [Q9XXK1-2]
NP_001021528.1. NM_001026357.6. [Q9XXK1-3]
NP_001021529.1. NM_001026358.4. [Q9XXK1-4]
UniGeneiCel.22874.

3D structure databases

ProteinModelPortaliQ9XXK1.
SMRiQ9XXK1. Positions 51-529.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38531. 3 interactions.
DIPiDIP-25446N.
IntActiQ9XXK1. 2 interactions.
MINTiMINT-213652.
STRINGi6239.H28O16.1a.

PTM databases

iPTMnetiQ9XXK1.

2D gel databases

World-2DPAGE0020:Q9XXK1.

Proteomic databases

EPDiQ9XXK1.
PaxDbiQ9XXK1.
PRIDEiQ9XXK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiH28O16.1a.1; H28O16.1a.1; WBGene00010419. [Q9XXK1-1]
H28O16.1a.2; H28O16.1a.2; WBGene00010419. [Q9XXK1-1]
H28O16.1a.3; H28O16.1a.3; WBGene00010419. [Q9XXK1-1]
H28O16.1a.4; H28O16.1a.4; WBGene00010419. [Q9XXK1-1]
H28O16.1a.5; H28O16.1a.5; WBGene00010419. [Q9XXK1-1]
GeneIDi173134.
KEGGicel:CELE_H28O16.1.
UCSCiH28O16.1a. c. elegans. [Q9XXK1-1]

Organism-specific databases

CTDi173134.
WormBaseiH28O16.1a; CE18826; WBGene00010419.
H28O16.1b; CE34194; WBGene00010419.
H28O16.1c; CE34195; WBGene00010419.
H28O16.1d; CE36263; WBGene00010419.

Phylogenomic databases

eggNOGiKOG1353. Eukaryota.
COG0056. LUCA.
HOGENOMiHOG000130111.
InParanoidiQ9XXK1.
KOiK02132.
OMAiQPMSISE.
PhylomeDBiQ9XXK1.

Miscellaneous databases

PROiQ9XXK1.

Family and domain databases

Gene3Di2.40.30.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01346. ATP_synth_alpha_bact.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR023366. ATPase_asu-like.
IPR005294. ATPase_F1-cplx_asu.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR15184:SF3. PTHR15184:SF3. 1 hit.
PfamiPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039088. F_ATPase_subunit_alpha. 1 hit.
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00962. atpA. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. Bienvenut W.V.
    Submitted (MAR-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 59-68; 119-134; 168-189; 193-199 AND 204-215 (ISOFORMS A/D), IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiATPA_CAEEL
AccessioniPrimary (citable) accession number: Q9XXK1
Secondary accession number(s): Q7K7K6, Q7YTU5, Q7YTU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.