Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable voltage-gated potassium channel subunit kvs-4

Gene

kvs-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Names & Taxonomyi

Protein namesi
Recommended name:
Probable voltage-gated potassium channel subunit kvs-4Curated
Gene namesi
Name:kvs-4Imported
ORF Names:Y48A6B.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY48A6B.6a; CE36457; WBGene00012967; kvs-4.
Y48A6B.6b; CE36458; WBGene00012967; kvs-4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 231CytoplasmicCuratedAdd BLAST231
Transmembranei232 – 252Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini253 – 275ExtracellularCuratedAdd BLAST23
Transmembranei276 – 296Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini297 – 313CytoplasmicCuratedAdd BLAST17
Transmembranei314 – 334Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini335 – 346ExtracellularCuratedAdd BLAST12
Transmembranei347 – 366Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST20
Topological domaini367 – 383CytoplasmicCuratedAdd BLAST17
Transmembranei384 – 404Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini405 – 417ExtracellularCuratedAdd BLAST13
Intramembranei418 – 429Helical; Name=Pore helixBy similarityAdd BLAST12
Intramembranei430 – 434By similarity5
Topological domaini435 – 445ExtracellularCuratedAdd BLAST11
Transmembranei446 – 466Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini467 – 503CytoplasmicCuratedAdd BLAST37

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi217 – 221WNIIE → AAAAA: Disrupts dendritic localization. 1 Publication5
Mutagenesisi217 – 219WNI → ANA: Disrupts dendritic localization. 1 Publication3
Mutagenesisi219I → A: Disrupts dendritic localization. 1 Publication1
Mutagenesisi496 – 500EQMIL → AAAAA: Disrupts dendritic localization. 1 Publication5
Mutagenesisi497 – 498QM → AA: Disrupts dendritic localization. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004408981 – 503Probable voltage-gated potassium channel subunit kvs-4CuratedAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi264N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6EUU0.

Expressioni

Tissue specificityi

Expressed in the cholinergic motor neuron DA9, mechanosensory neurons ALM and PLM, and the interneuron PVPL.1 Publication

Gene expression databases

BgeeiWBGene00012967.
ExpressionAtlasiQ9XXD1. baseline.

Interactioni

Subunit structurei

Homotetramer or heterotetramer (Probable). Interacts with unc-101 (via N-terminus); which targets kvs-4 to dendrites (PubMed:26762178).Curated1 Publication

Protein-protein interaction databases

IntActiQ9XXD1. 1 interactor.
STRINGi6239.Y48A6B.6b.

Structurei

3D structure databases

ProteinModelPortaliQ9XXD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 383S4-S5 linkerBy similarityAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi217 – 219Required for dendritic localization1 Publication3
Motifi430 – 435Selectivity filterBy similarity6
Motifi496 – 500Required for dendritic localization1 Publication5

Domaini

The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity
The EQMIL and WNIIE motifs are required for dendritic localization.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00890000139669.
HOGENOMiHOG000272628.
InParanoidiQ6EUU0.
OMAiNHFHIPT.
OrthoDBiEOG091G0FP3.

Family and domain databases

InterProiView protein in InterPro
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR011333. SKP1/BTB/POZ_sf.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiView protein in Pfam
PF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: Q9XXD1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSAIMQGAA MATSHGIARL NRHNAQRNHF HIPTANRLFY QLQTPCTSTE
60 70 80 90 100
EERRTVQTDD VAEIGMQRPD YQMYCGEEEI SEQFVKLNVG GQRFMLRKDT
110 120 130 140 150
IRRRGVGRLL DLINKPVADS NADAFFSSTS EFYFERPPSL FHIVYQFYLN
160 170 180 190 200
GVIHQPSNLC PVDIIEELEY WRIIPDQYLA SCCCAQQIDD DDEEVEEQDK
210 220 230 240 250
PNLFKTLRFG EIRRCVWNII EEPASSGKAQ AFAVCSVVFV LISISGLVLG
260 270 280 290 300
SLPELQVATK QRNNLTGEEF TEMEPMPILG YIEYVCIVWF TMEYGLKMLV
310 320 330 340 350
SAERSKTFRQ LLNIIDLLAI LPFIIEMLLL IFGISTEQLR DLKGAFLVIR
360 370 380 390 400
ILRVLRVIRV LKLGRYSSGL QMFGKTLKAS FRQLGMMAMV VMTGVIFFST
410 420 430 440 450
LVYFLEKDEP ASKFHSIPAA CWWCIVTMTT VGYGDLTPVT VPGKLVATGA
460 470 480 490 500
IACGVLVLAL PITIIVDNFM KVAETERPAG GNRYRTSQYP KATKSEQMIL

KVT
Length:503
Mass (Da):56,966
Last modified:September 13, 2004 - v3
Checksum:i1611A5E0417E016C
GO
Isoform bImported (identifier: Q9XXD1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: MNSAIMQGAA...LFYQLQTPCT → MSHQASHFGV...DMVSQSSLDR
     452-478: Missing.

Show »
Length:512
Mass (Da):57,965
Checksum:iFEA4D7F9F8548D79
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0590071 – 47MNSAI…QTPCT → MSHQASHFGVKKKWKANGSS GGGGLSAFARMKPVLDPSAD RIWTIGQIIYQRHQAFNRNS AIKSQDNGSFDYIDMVSQSS LDR in isoform b. Add BLAST47
Alternative sequenceiVSP_059008452 – 478Missing in isoform b. Add BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA. Translation: CAA19530.3.
BX284603 Genomic DNA. Translation: CAH04760.1.
PIRiT26983.
RefSeqiNP_001022888.1. NM_001027717.2. [Q9XXD1-1]
NP_001022889.1. NM_001027718.3. [Q9XXD1-2]
UniGeneiCel.9760.

Genome annotation databases

EnsemblMetazoaiY48A6B.6a; Y48A6B.6a; WBGene00012967. [Q9XXD1-1]
Y48A6B.6b; Y48A6B.6b; WBGene00012967. [Q9XXD1-2]
GeneIDi190011.
KEGGicel:CELE_Y48A6B.6.
UCSCiY48A6B.6b. c. elegans. [Q9XXD1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKVS4_CAEEL
AccessioniPrimary (citable) accession number: Q9XXD1
Secondary accession number(s): Q6EUU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2017
Last sequence update: September 13, 2004
Last modified: November 22, 2017
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families