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Protein

Probable 5-formyltetrahydrofolate cyclo-ligase

Gene

Y106G6E.4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-CHO-H4PteGlu) to yield 5,10-methenyltetrahydrofolate (By similarity).By similarity

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATPBy similarity
Binding sitei54 – 541Substrate; via carbonyl oxygenBy similarity
Binding sitei59 – 591SubstrateBy similarity
Metal bindingi152 – 1521MagnesiumBy similarity
Metal bindingi188 – 1881MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 125ATPBy similarity
Nucleotide bindingi143 – 1519ATPBy similarity

GO - Molecular functioni

  1. 5-formyltetrahydrofolate cyclo-ligase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Alternative name(s):
5,10-methenyl-tetrahydrofolate synthetase
Short name:
MTHFS
Short name:
Methenyl-THF synthetase
Gene namesi
ORF Names:Y106G6E.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiY106G6E.4; CE20408; WBGene00013708.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Probable 5-formyltetrahydrofolate cyclo-ligasePRO_0000200278Add
BLAST

Proteomic databases

PaxDbiQ9XWE6.
PRIDEiQ9XWE6.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi6239.Y106G6E.4.

Structurei

3D structure databases

ProteinModelPortaliQ9XWE6.
SMRiQ9XWE6. Positions 2-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni146 – 1505Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0212.
GeneTreeiENSGT00390000017791.
HOGENOMiHOG000007299.
InParanoidiQ9XWE6.
KOiK01934.
OMAiSCYLSMP.
OrthoDBiEOG73806H.
PhylomeDBiQ9XWE6.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XWE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAIKEVKSE LRQFMKTLLG KISKEETQRQ TEAVFEKIIE SKWFQESKRL
60 70 80 90 100
SVYVSTSGEI QTDSIIQKAL EMGKEVFIPQ FTKGSTAMDM VRVPDQTAFD
110 120 130 140 150
NLPSTLWGIR QPEPKWKWQS YHETGPLDLI LAPGVAFSPY GLRCGHGKGY
160 170 180 190 200
YDRFFSTHHK HFPENSPKKI GLALREQIIG TIPISETDVE LDEVIYEGET

IIFDTI
Length:206
Mass (Da):23,561
Last modified:November 1, 1999 - v1
Checksum:iCAA2EF7A31F13039
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL032656 Genomic DNA. Translation: CAA21728.1.
PIRiT26418.
RefSeqiNP_492692.1. NM_060291.5.
UniGeneiCel.8713.

Genome annotation databases

EnsemblMetazoaiY106G6E.4; Y106G6E.4; WBGene00013708.
GeneIDi190922.
KEGGicel:CELE_Y106G6E.4.
UCSCiY106G6E.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL032656 Genomic DNA. Translation: CAA21728.1.
PIRiT26418.
RefSeqiNP_492692.1. NM_060291.5.
UniGeneiCel.8713.

3D structure databases

ProteinModelPortaliQ9XWE6.
SMRiQ9XWE6. Positions 2-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.Y106G6E.4.

Proteomic databases

PaxDbiQ9XWE6.
PRIDEiQ9XWE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY106G6E.4; Y106G6E.4; WBGene00013708.
GeneIDi190922.
KEGGicel:CELE_Y106G6E.4.
UCSCiY106G6E.4. c. elegans.

Organism-specific databases

CTDi190922.
WormBaseiY106G6E.4; CE20408; WBGene00013708.

Phylogenomic databases

eggNOGiCOG0212.
GeneTreeiENSGT00390000017791.
HOGENOMiHOG000007299.
InParanoidiQ9XWE6.
KOiK01934.
OMAiSCYLSMP.
OrthoDBiEOG73806H.
PhylomeDBiQ9XWE6.

Miscellaneous databases

NextBioi947390.
PROiQ9XWE6.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiMTHFS_CAEEL
AccessioniPrimary (citable) accession number: Q9XWE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.