Q9XW79 (PP4C1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit 1
Short name=PP4C-1
EC=3.1.3.16

Gene names

Name:	pph-4.1
ORF Names:	Y75B8A.30

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein phosphatase which is essential for embryogenesis. Is required for centrosome maturation and is involved in gametogenesis. Required for meiotic spindle formation during spermatogenesis and for chiasmata formation during oogenesis. However, is apparently dispensable for female meiotic divisions, which do not depend on centrosomes. Ref.1
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits By similarity.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Note: Localizes at centrosomes from prophase to telophase but dot during interphase. Locolaized to cytoplasm throughout the cell cycle. Ref.1
Sequence similarities	Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
Sequence caution	The sequence BAB63947.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ppfr-4	Q9N4E9	3	EBI-331742,EBI-331766

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Serine/threonine-protein phosphatase 4 catalytic subunit 1		PRO_0000353210
Sites
	Active site	140	1	Proton donor By similarity
	Metal binding	79	1	Iron By similarity
	Metal binding	81	1	Iron By similarity
	Metal binding	107	1	Iron By similarity
	Metal binding	107	1	Manganese By similarity
	Metal binding	139	1	Manganese By similarity
	Metal binding	189	1	Manganese By similarity
	Metal binding	264	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XW79 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 7C7EA2177E45AA52	FASTA	333	37,359
	10 20 30 40 50 60 MALACTDSAN STFSRVDSPT SGPSDQLTTH DLDRHIEKLM RCELIAEQDV KTLCAKAREI 70 80 90 100 110 120 LAEEGNVQVI DSPVTICGDI HGQFYDLMEL FKVGGPVPNT NYLFLGDFVD RGFYSVETFL 130 140 150 160 170 180 LLLALKARYP DRMMLIRGNH ESRQITQVYG FYDECLRKYG NASVWKHCTE VFDYLSLAAV 190 200 210 220 230 240 IDGKVFCVHG GLSPSISTMD QIRVIDRKQE VPHDGPMCDL LWSDPEEGNV GWGLSPRGAG 250 260 270 280 290 300 YLFGADASKT FCETNGVDLI CRAHQLVMEG YKWHFNEKVL TVWSAPNYCY RCGNVAAILE 310 320 330 LDENLNKEFT IFEAAPQENR GAPAKKPHAD YFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein phosphatase 4 is required for centrosome maturation in mitosis and sperm meiosis in C. elegans." Sumiyoshi E., Sugimoto A., Yamamoto M. J. Cell Sci. 115:1403-1410(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB070573 mRNA. Translation: BAB63947.1. Different initiation. AL033514 Genomic DNA. Translation: CAA22090.1.
PIR	T27390.
RefSeq	NP_499603.1. NM_067202.5.
UniGene	Cel.19352.
3D structure databases
ProteinModelPortal	Q9XW79.
SMR	Q9XW79. Positions 29-316.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24807N.
IntAct	Q9XW79. 7 interactions.
MINT	MINT-1045242.
Proteomic databases
PaxDb	Q9XW79.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y75B8A.30; Y75B8A.30; Y75B8A.30.
GeneID	176657.
KEGG	cel:CELE_Y75B8A.30.
UCSC	Y75B8A.30. c. elegans.
Organism-specific databases
CTD	176657.
WormBase	Y75B8A.30; CE23041; WBGene00004085; pph-4.1.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00550000074618.
HOGENOM	HOG000172696.
InParanoid	Q9XW79.
KO	K15423.
OMA	GFKWHFN.
Enzyme and pathway databases
SignaLink	Q9XW79.
Gene expression databases
ArrayExpress	Q9XW79.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	893488.

Entry information

Entry name

PP4C1_CAEEL

Accession

Primary (citable) accession number: Q9XW79
Secondary accession number(s): Q966Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	November 1, 1999
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project