Reviewed, UniProtKB/Swiss-Prot Q9XVS1 (MCES_CAEEL)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase

Gene names

Name:	tag-72
ORF Names:	C25A1.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB mRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	mRNA cap guanine-N7 methyltransferase		PRO_0000248329
Regions
	Region	33 – 34	2	mRNA cap binding By similarity
Sites
	Binding site	37	1	S-adenosyl-L-methionine By similarity
	Binding site	62	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	65	1	mRNA cap By similarity
	Binding site	71	1	mRNA cap By similarity
	Binding site	84	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	mRNA cap By similarity
	Binding site	117	1	S-adenosyl-L-methionine By similarity
	Binding site	139	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	143	1	mRNA cap By similarity
	Binding site	230	1	mRNA cap By similarity
	Binding site	325	1	mRNA cap By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XVS1-1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: B6613B76804EB533	FASTA	380	43,312
	10 20 30 40 50 60 MSSSEVASHY NKVLQVGIEG RKESRIFFMR NMNNWVKSQL INDAKQRVND NGVNNPRVLD 70 80 90 100 110 120 LACGKGGDLK KWDIAGAKDV VMADVADVSI QQAEERYKQM FGYKKNNIFT VQFIVADCTK 130 140 150 160 170 180 ENLEDRIENK DPFDLVSCQF ALHYSFVDEA SARIFLKNAV GMLKPGGVFI GTLPDADRIV 190 200 210 220 230 240 WSMRNGENGQ FANEVCKITY ENVEELAEGK VPLFGAKFHF SLDEQVNCPE FLAYFPLVKH 250 260 270 280 290 300 LLEELDMELL FVHNFAEAIN KWLEPGRRLL ESMTGLETYP NEKLSGKSDD EYLEAKAKLD 310 320 330 340 350 360 AFPEDERIKT MGTLSKSEWE AICMYLVFGF RKKKSEAEKT EEEPATTKPV AESESEQKEV 370 380 TESEEKEDQE DCEHQEAQTN

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z81038 Genomic DNA. Translation: CAB02758.2.
PIR	T19434.
RefSeq	NP_492674.2.
UniGene	Cel.19202
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XVS1.
Genome annotation databases
Ensembl	C25A1.3; C25A1.3; C25A1.3; Caenorhabditis elegans. [Genome view]
GeneID	182875.
KEGG	cel:C25A1.3.
NMPDR	fig|6239.3.peg.2715.
UCSC	C25A1.3. c. elegans.
Organism-specific databases
CTD	182875.
WormBase	WBGene00006447. tag-72.
WormPep	C25A1.3. CE36918. [WorfDB]
Phylogenomic databases
OMA	FPARLIC.
Enzyme and pathway databases
BRENDA	2.1.1.56. 672.
Gene expression databases
ArrayExpress	Q9XVS1.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	919146.

Entry information

Entry name

MCES_CAEEL

Accession

Primary (citable) accession number: Q9XVS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 13, 2004
Last modified:	November 3, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project