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Q9XVR6 (OTUBL_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase otubain-like

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme otubain-like
Ubiquitin-specific-processing protease otubain-like
Gene names
Name:otub-1
ORF Names:C25D7.8
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Specifically cleaves 'Lys-48'-linked polyubiquitin. Ref.2

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C65 family.

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284Ubiquitin thioesterase otubain-like
PRO_0000221013

Regions

Domain77 – 274198OTU

Sites

Active site851 By similarity
Active site881Nucleophile By similarity
Active site2671 By similarity

Secondary structure

........................................ 284
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9XVR6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 262500E41F8FA23C

FASTA28432,293
        10         20         30         40         50         60 
MANEPQKSDD NGQAAEAVVT DDEIVLQDQQ LKTIEDEQKS VPLVATLAPF SILCAEYDNE 

        70         80         90        100        110        120 
TSAAFLSKAT ELSEVYGEIR YIRGDGNCFY RAILVGLIEI MLKDRARLEK FIASSRDWTR 

       130        140        150        160        170        180 
TLVELGFPDW TCTDFCDFFI EFLEKIHSGV HTEEAVYTIL NDDGSANYIL MFFRLITSAF 

       190        200        210        220        230        240 
LKQNSEEYAP FIDEGMTVAQ YCEQEIEPMW KDADHLAINS LIKAAGTRVR IEYMDRTAAP 

       250        260        270        280 
NGGWHYDIPS DDQQIAPEIT LLYRPGHYDV IYKKDSTEAS EIEN 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1."
Wang T., Yin L., Cooper E.M., Lai M.-Y., Dickey S., Pickart C.M., Fushman D., Wilkinson K.D., Cohen R.E., Wolberger C.
J. Mol. Biol. 386:1011-1023(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z81039 Genomic DNA. Translation: CAB02772.1.
PIRT19448.
RefSeqNP_506709.1. NM_074308.4.
UniGeneCel.4680.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DHIX-ray1.80B1-284[»]
4DHJX-ray2.35A/E/I/L1-284[»]
4DHZX-ray3.11A42-284[»]
4LDTX-ray1.90A42-284[»]
ProteinModelPortalQ9XVR6.
SMRQ9XVR6. Positions 28-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid533138. 2 interactions.
DIPDIP-26135N.
IntActQ9XVR6. 2 interactions.
MINTMINT-1069399.
STRING6239.C25D7.8.

Protein family/group databases

MEROPSC65.A01.

Proteomic databases

PaxDbQ9XVR6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC25D7.8; C25D7.8; WBGene00007718.
GeneID3565820.
KEGGcel:CELE_C25D7.8.
UCSCC25D7.8. c. elegans.

Organism-specific databases

CTD3565820.
WormBaseC25D7.8; CE08394; WBGene00007718; otub-1.

Phylogenomic databases

eggNOGCOG5539.
GeneTreeENSGT00390000006979.
HOGENOMHOG000019496.
InParanoidQ9XVR6.
KOK09602.
OMAVEPMYKE.
PhylomeDBQ9XVR6.

Family and domain databases

InterProIPR003323. OTU.
IPR019400. Peptidase_C65_otubain.
IPR016615. Ubiquitin_thioesterase_Otubain.
[Graphical view]
PfamPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio957977.
PROQ9XVR6.

Entry information

Entry nameOTUBL_CAEEL
AccessionPrimary (citable) accession number: Q9XVR6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase