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Q9XVR6

- OTUBL_CAEEL

UniProt

Q9XVR6 - OTUBL_CAEEL

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Protein

Ubiquitin thioesterase otubain-like

Gene

otub-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Specifically cleaves 'Lys-48'-linked polyubiquitin.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851By similarity
Active sitei88 – 881NucleophileBy similarity
Active sitei267 – 2671By similarity

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
  2. ubiquitin-specific protease activity Source: WormBase

GO - Biological processi

  1. cellular amino acid metabolic process Source: UniProtKB
  2. protein K48-linked deubiquitination Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC65.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase otubain-like (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme otubain-like
Ubiquitin-specific-processing protease otubain-like
Gene namesi
Name:otub-1
ORF Names:C25D7.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiC25D7.8; CE08394; WBGene00007718; otub-1.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Ubiquitin thioesterase otubain-likePRO_0000221013Add
BLAST

Proteomic databases

PaxDbiQ9XVR6.

Interactioni

Protein-protein interaction databases

BioGridi533138. 2 interactions.
DIPiDIP-26135N.
IntActiQ9XVR6. 2 interactions.
MINTiMINT-1069399.
STRINGi6239.C25D7.8.

Structurei

Secondary structure

1
284
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 378
Helixi38 – 403
Helixi50 – 556
Turni59 – 613
Helixi63 – 7311
Beta strandi76 – 805
Helixi88 – 10316
Helixi105 – 12420
Helixi129 – 14719
Beta strandi149 – 1513
Helixi153 – 1619
Helixi163 – 18321
Helixi185 – 1884
Helixi189 – 1913
Helixi198 – 2058
Helixi215 – 22410
Beta strandi229 – 2357
Helixi236 – 2383
Beta strandi242 – 2498
Beta strandi258 – 2647
Beta strandi267 – 2737

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DHIX-ray1.80B1-284[»]
4DHJX-ray2.35A/E/I/L1-284[»]
4DHZX-ray3.11A42-284[»]
4LDTX-ray1.90A42-284[»]
ProteinModelPortaliQ9XVR6.
SMRiQ9XVR6. Positions 28-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 274198OTUPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C65 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000006979.
HOGENOMiHOG000019496.
InParanoidiQ9XVR6.
KOiK09602.
OMAiVEPMYKE.
PhylomeDBiQ9XVR6.

Family and domain databases

InterProiIPR003323. OTU.
IPR019400. Peptidase_C65_otubain.
IPR016615. Ubiquitin_thioesterase_Otubain.
[Graphical view]
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XVR6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANEPQKSDD NGQAAEAVVT DDEIVLQDQQ LKTIEDEQKS VPLVATLAPF
60 70 80 90 100
SILCAEYDNE TSAAFLSKAT ELSEVYGEIR YIRGDGNCFY RAILVGLIEI
110 120 130 140 150
MLKDRARLEK FIASSRDWTR TLVELGFPDW TCTDFCDFFI EFLEKIHSGV
160 170 180 190 200
HTEEAVYTIL NDDGSANYIL MFFRLITSAF LKQNSEEYAP FIDEGMTVAQ
210 220 230 240 250
YCEQEIEPMW KDADHLAINS LIKAAGTRVR IEYMDRTAAP NGGWHYDIPS
260 270 280
DDQQIAPEIT LLYRPGHYDV IYKKDSTEAS EIEN
Length:284
Mass (Da):32,293
Last modified:November 1, 1999 - v1
Checksum:i262500E41F8FA23C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z81039 Genomic DNA. Translation: CAB02772.1.
PIRiT19448.
RefSeqiNP_506709.1. NM_074308.4.
UniGeneiCel.4680.

Genome annotation databases

EnsemblMetazoaiC25D7.8; C25D7.8; WBGene00007718.
GeneIDi3565820.
KEGGicel:CELE_C25D7.8.
UCSCiC25D7.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z81039 Genomic DNA. Translation: CAB02772.1 .
PIRi T19448.
RefSeqi NP_506709.1. NM_074308.4.
UniGenei Cel.4680.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DHI X-ray 1.80 B 1-284 [» ]
4DHJ X-ray 2.35 A/E/I/L 1-284 [» ]
4DHZ X-ray 3.11 A 42-284 [» ]
4LDT X-ray 1.90 A 42-284 [» ]
ProteinModelPortali Q9XVR6.
SMRi Q9XVR6. Positions 28-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 533138. 2 interactions.
DIPi DIP-26135N.
IntActi Q9XVR6. 2 interactions.
MINTi MINT-1069399.
STRINGi 6239.C25D7.8.

Protein family/group databases

MEROPSi C65.A01.

Proteomic databases

PaxDbi Q9XVR6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C25D7.8 ; C25D7.8 ; WBGene00007718 .
GeneIDi 3565820.
KEGGi cel:CELE_C25D7.8.
UCSCi C25D7.8. c. elegans.

Organism-specific databases

CTDi 3565820.
WormBasei C25D7.8 ; CE08394 ; WBGene00007718 ; otub-1.

Phylogenomic databases

eggNOGi COG5539.
GeneTreei ENSGT00390000006979.
HOGENOMi HOG000019496.
InParanoidi Q9XVR6.
KOi K09602.
OMAi VEPMYKE.
PhylomeDBi Q9XVR6.

Miscellaneous databases

NextBioi 957977.
PROi Q9XVR6.

Family and domain databases

InterProi IPR003323. OTU.
IPR019400. Peptidase_C65_otubain.
IPR016615. Ubiquitin_thioesterase_Otubain.
[Graphical view ]
Pfami PF10275. Peptidase_C65. 1 hit.
[Graphical view ]
PIRSFi PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1."
    Wang T., Yin L., Cooper E.M., Lai M.-Y., Dickey S., Pickart C.M., Fushman D., Wilkinson K.D., Cohen R.E., Wolberger C.
    J. Mol. Biol. 386:1011-1023(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiOTUBL_CAEEL
AccessioniPrimary (citable) accession number: Q9XVR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: November 1, 1999
Last modified: October 1, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3