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Q9XVR6

- OTUBL_CAEEL

UniProt

Q9XVR6 - OTUBL_CAEEL

Protein

Ubiquitin thioesterase otubain-like

Gene

otub-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Specifically cleaves 'Lys-48'-linked polyubiquitin.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851By similarity
    Active sitei88 – 881NucleophileBy similarity
    Active sitei267 – 2671By similarity

    GO - Molecular functioni

    1. omega peptidase activity Source: InterPro
    2. ubiquitin-specific protease activity Source: WormBase

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB
    2. protein K48-linked deubiquitination Source: WormBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC65.A01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase otubain-like (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme otubain-like
    Ubiquitin-specific-processing protease otubain-like
    Gene namesi
    Name:otub-1
    ORF Names:C25D7.8
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiC25D7.8; CE08394; WBGene00007718; otub-1.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Ubiquitin thioesterase otubain-likePRO_0000221013Add
    BLAST

    Proteomic databases

    PaxDbiQ9XVR6.

    Interactioni

    Protein-protein interaction databases

    BioGridi533138. 2 interactions.
    DIPiDIP-26135N.
    IntActiQ9XVR6. 2 interactions.
    MINTiMINT-1069399.
    STRINGi6239.C25D7.8.

    Structurei

    Secondary structure

    1
    284
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 378
    Helixi38 – 403
    Helixi50 – 556
    Turni59 – 613
    Helixi63 – 7311
    Beta strandi76 – 805
    Helixi88 – 10316
    Helixi105 – 12420
    Helixi129 – 14719
    Beta strandi149 – 1513
    Helixi153 – 1619
    Helixi163 – 18321
    Helixi185 – 1884
    Helixi189 – 1913
    Helixi198 – 2058
    Helixi215 – 22410
    Beta strandi229 – 2357
    Helixi236 – 2383
    Beta strandi242 – 2498
    Beta strandi258 – 2647
    Beta strandi267 – 2737

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4DHIX-ray1.80B1-284[»]
    4DHJX-ray2.35A/E/I/L1-284[»]
    4DHZX-ray3.11A42-284[»]
    4LDTX-ray1.90A42-284[»]
    ProteinModelPortaliQ9XVR6.
    SMRiQ9XVR6. Positions 28-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 274198OTUPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C65 family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5539.
    GeneTreeiENSGT00390000006979.
    HOGENOMiHOG000019496.
    InParanoidiQ9XVR6.
    KOiK09602.
    OMAiVEPMYKE.
    PhylomeDBiQ9XVR6.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view]
    PfamiPF10275. Peptidase_C65. 1 hit.
    [Graphical view]
    PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XVR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANEPQKSDD NGQAAEAVVT DDEIVLQDQQ LKTIEDEQKS VPLVATLAPF    50
    SILCAEYDNE TSAAFLSKAT ELSEVYGEIR YIRGDGNCFY RAILVGLIEI 100
    MLKDRARLEK FIASSRDWTR TLVELGFPDW TCTDFCDFFI EFLEKIHSGV 150
    HTEEAVYTIL NDDGSANYIL MFFRLITSAF LKQNSEEYAP FIDEGMTVAQ 200
    YCEQEIEPMW KDADHLAINS LIKAAGTRVR IEYMDRTAAP NGGWHYDIPS 250
    DDQQIAPEIT LLYRPGHYDV IYKKDSTEAS EIEN 284
    Length:284
    Mass (Da):32,293
    Last modified:November 1, 1999 - v1
    Checksum:i262500E41F8FA23C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z81039 Genomic DNA. Translation: CAB02772.1.
    PIRiT19448.
    RefSeqiNP_506709.1. NM_074308.4.
    UniGeneiCel.4680.

    Genome annotation databases

    EnsemblMetazoaiC25D7.8; C25D7.8; WBGene00007718.
    GeneIDi3565820.
    KEGGicel:CELE_C25D7.8.
    UCSCiC25D7.8. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z81039 Genomic DNA. Translation: CAB02772.1 .
    PIRi T19448.
    RefSeqi NP_506709.1. NM_074308.4.
    UniGenei Cel.4680.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4DHI X-ray 1.80 B 1-284 [» ]
    4DHJ X-ray 2.35 A/E/I/L 1-284 [» ]
    4DHZ X-ray 3.11 A 42-284 [» ]
    4LDT X-ray 1.90 A 42-284 [» ]
    ProteinModelPortali Q9XVR6.
    SMRi Q9XVR6. Positions 28-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 533138. 2 interactions.
    DIPi DIP-26135N.
    IntActi Q9XVR6. 2 interactions.
    MINTi MINT-1069399.
    STRINGi 6239.C25D7.8.

    Protein family/group databases

    MEROPSi C65.A01.

    Proteomic databases

    PaxDbi Q9XVR6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C25D7.8 ; C25D7.8 ; WBGene00007718 .
    GeneIDi 3565820.
    KEGGi cel:CELE_C25D7.8.
    UCSCi C25D7.8. c. elegans.

    Organism-specific databases

    CTDi 3565820.
    WormBasei C25D7.8 ; CE08394 ; WBGene00007718 ; otub-1.

    Phylogenomic databases

    eggNOGi COG5539.
    GeneTreei ENSGT00390000006979.
    HOGENOMi HOG000019496.
    InParanoidi Q9XVR6.
    KOi K09602.
    OMAi VEPMYKE.
    PhylomeDBi Q9XVR6.

    Miscellaneous databases

    NextBioi 957977.
    PROi Q9XVR6.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view ]
    Pfami PF10275. Peptidase_C65. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1."
      Wang T., Yin L., Cooper E.M., Lai M.-Y., Dickey S., Pickart C.M., Fushman D., Wilkinson K.D., Cohen R.E., Wolberger C.
      J. Mol. Biol. 386:1011-1023(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiOTUBL_CAEEL
    AccessioniPrimary (citable) accession number: Q9XVR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3