Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9XVI6

- SPTC3_CAEEL

UniProt

Q9XVI6 - SPTC3_CAEEL

Protein

Serine palmitoyltransferase 3

Gene

sptl-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the serine palmitoyltransferase (SPT) that catalyzes the first committed step in sphingolipid biosynthesis, which is the condensation of an acyl-CoA species and L-serine. The catalytic core is composed of a heterodimer of sptl-1 and sptl-2 or sptl-1 and sptl-3 By similarity. Required for the specification of abicobasal polarity and development of the gut lumen.By similarity1 Publication

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. sphingolipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_183003. Sphingolipid de novo biosynthesis.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 3 (EC:2.3.1.50)
    Alternative name(s):
    Long chain base biosynthesis protein 3
    Short name:
    LCB 3
    Serine-palmitoyl-CoA transferase 3
    Short name:
    SPT 3
    Short name:
    SPT3
    Gene namesi
    Name:sptl-3
    ORF Names:T22G5.5
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiT22G5.5a; CE31996; WBGene00011932; sptl-3.
    T22G5.5b; CE47328; WBGene00011932; sptl-3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 512512Serine palmitoyltransferase 3PRO_0000421273Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei345 – 3451N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PaxDbiQ9XVI6.

    Interactioni

    Subunit structurei

    Heterodimer of sptl-1/sptl-3.By similarity

    Protein-protein interaction databases

    DIPiDIP-26788N.
    IntActiQ9XVI6. 1 interaction.
    MINTiMINT-1127619.
    STRINGi6239.T22G5.5.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XVI6.
    SMRiQ9XVI6. Positions 133-502.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0156.
    GeneTreeiENSGT00550000074678.
    HOGENOMiHOG000206826.
    InParanoidiQ9XVI6.
    OMAiGINCGGS.
    PhylomeDBiQ9XVI6.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q9XVI6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGGTQNGKAV ANGKAKNGNI TEKVIKLDPC PKPAFYVFWL VQLNITMMLV    50
    GAMVATLFDK WGIVKTKRSK GDPRMESFQP LGNSFDATYT DHIYRQSTDV 100
    VNRPISGVPG AIVRLKDRYT DDHGWTQKYT GTESEVINLG SYNYLGFSHR 150
    SGVCAEAAAA HIDKYGINCG GSRQEIGNHV AHKSVESTIA QYLNVEDAIV 200
    FPMGFATNSM NIPSLVDKGS LILSDRLNHA SLVTGCRLSG AHTVVFRHND 250
    ASDCERKLRD ALCGVSPKTG EKYNKVLIII EGIYSMEGTI VNLPAFIAVK 300
    KKYNCYLFLD EAHSIGAVGP SGRGVAEYWG CNPRDIDIMM GTLTKSFASA 350
    GGYMGGSKKV IDHIRRYSAG TCYGVTMSPP LIAQVERAVL IMSGKDGTDI 400
    GRQKAIQLLE NSRYFRKELR KRGFLVYGNN DSPVVPLMTF YITKVVEFSR 450
    RMLKHNIGIV AVGYPATPLL EARVRFCLSA DHTKEHLDYI LEAVEQVGME 500
    TGTFYGTKIV DE 512
    Length:512
    Mass (Da):56,334
    Last modified:March 1, 2003 - v2
    Checksum:i3F68835D2961C4D0
    GO
    Isoform b (identifier: Q9XVI6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MSSCFVFYNM

    Show »
    Length:521
    Mass (Da):57,413
    Checksum:i0D69BF15189AF627
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MSSCFVFYNM in isoform b. CuratedVSP_045380

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z81127 Genomic DNA. Translation: CAB03390.2.
    Z81127 Genomic DNA. Translation: CCG28222.1.
    PIRiT25126.
    RefSeqiNP_001256547.1. NM_001269618.1. [Q9XVI6-2]
    NP_001256548.1. NM_001269619.1. [Q9XVI6-1]
    UniGeneiCel.17398.

    Genome annotation databases

    EnsemblMetazoaiT22G5.5a.1; T22G5.5a.1; WBGene00011932. [Q9XVI6-1]
    T22G5.5a.2; T22G5.5a.2; WBGene00011932. [Q9XVI6-1]
    GeneIDi179884.
    KEGGicel:CELE_T22G5.5.
    UCSCiT22G5.5.1. c. elegans. [Q9XVI6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z81127 Genomic DNA. Translation: CAB03390.2 .
    Z81127 Genomic DNA. Translation: CCG28222.1 .
    PIRi T25126.
    RefSeqi NP_001256547.1. NM_001269618.1. [Q9XVI6-2 ]
    NP_001256548.1. NM_001269619.1. [Q9XVI6-1 ]
    UniGenei Cel.17398.

    3D structure databases

    ProteinModelPortali Q9XVI6.
    SMRi Q9XVI6. Positions 133-502.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-26788N.
    IntActi Q9XVI6. 1 interaction.
    MINTi MINT-1127619.
    STRINGi 6239.T22G5.5.1.

    Proteomic databases

    PaxDbi Q9XVI6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T22G5.5a.1 ; T22G5.5a.1 ; WBGene00011932 . [Q9XVI6-1 ]
    T22G5.5a.2 ; T22G5.5a.2 ; WBGene00011932 . [Q9XVI6-1 ]
    GeneIDi 179884.
    KEGGi cel:CELE_T22G5.5.
    UCSCi T22G5.5.1. c. elegans. [Q9XVI6-1 ]

    Organism-specific databases

    CTDi 179884.
    WormBasei T22G5.5a ; CE31996 ; WBGene00011932 ; sptl-3.
    T22G5.5b ; CE47328 ; WBGene00011932 ; sptl-3.

    Phylogenomic databases

    eggNOGi COG0156.
    GeneTreei ENSGT00550000074678.
    HOGENOMi HOG000206826.
    InParanoidi Q9XVI6.
    OMAi GINCGGS.
    PhylomeDBi Q9XVI6.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    Reactomei REACT_183003. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    NextBioi 907264.
    PROi Q9XVI6.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis."
      Zhang H., Abraham N., Khan L.A., Hall D.H., Fleming J.T., Gobel V.
      Nat. Cell Biol. 13:1189-1201(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSPTC3_CAEEL
    AccessioniPrimary (citable) accession number: Q9XVI6
    Secondary accession number(s): H9G307
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2013
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3