Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9XVI6 (SPTC3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine palmitoyltransferase 3

EC=2.3.1.50
Alternative name(s):
Long chain base biosynthesis protein 3
Short name=LCB 3
Serine-palmitoyl-CoA transferase 3
Short name=SPT 3
Short name=SPT3
Gene names
Name:sptl-3
ORF Names:T22G5.5
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the serine palmitoyltransferase (SPT) that catalyzes the first committed step in sphingolipid biosynthesis, which is the condensation of an acyl-CoA species and L-serine. The catalytic core is composed of a heterodimer of sptl-1 and sptl-2 or sptl-1 and sptl-3 By similarity. Required for the specification of abicobasal polarity and development of the gut lumen. Ref.2

Catalytic activity

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Lipid metabolism; sphingolipid metabolism.

Subunit structure

Heterodimer of sptl-1/sptl-3 By similarity.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q9XVI6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q9XVI6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSSCFVFYNM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Serine palmitoyltransferase 3
PRO_0000421273

Amino acid modifications

Modified residue3451N6-(pyridoxal phosphate)lysine By similarity

Natural variations

Alternative sequence11M → MSSCFVFYNM in isoform b.
VSP_045380

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified March 1, 2003. Version 2.
Checksum: 3F68835D2961C4D0

FASTA51256,334
        10         20         30         40         50         60 
MGGTQNGKAV ANGKAKNGNI TEKVIKLDPC PKPAFYVFWL VQLNITMMLV GAMVATLFDK 

        70         80         90        100        110        120 
WGIVKTKRSK GDPRMESFQP LGNSFDATYT DHIYRQSTDV VNRPISGVPG AIVRLKDRYT 

       130        140        150        160        170        180 
DDHGWTQKYT GTESEVINLG SYNYLGFSHR SGVCAEAAAA HIDKYGINCG GSRQEIGNHV 

       190        200        210        220        230        240 
AHKSVESTIA QYLNVEDAIV FPMGFATNSM NIPSLVDKGS LILSDRLNHA SLVTGCRLSG 

       250        260        270        280        290        300 
AHTVVFRHND ASDCERKLRD ALCGVSPKTG EKYNKVLIII EGIYSMEGTI VNLPAFIAVK 

       310        320        330        340        350        360 
KKYNCYLFLD EAHSIGAVGP SGRGVAEYWG CNPRDIDIMM GTLTKSFASA GGYMGGSKKV 

       370        380        390        400        410        420 
IDHIRRYSAG TCYGVTMSPP LIAQVERAVL IMSGKDGTDI GRQKAIQLLE NSRYFRKELR 

       430        440        450        460        470        480 
KRGFLVYGNN DSPVVPLMTF YITKVVEFSR RMLKHNIGIV AVGYPATPLL EARVRFCLSA 

       490        500        510 
DHTKEHLDYI LEAVEQVGME TGTFYGTKIV DE 

« Hide

Isoform b [UniParc].

Checksum: 0D69BF15189AF627
Show »

FASTA52157,413

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[2]"Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis."
Zhang H., Abraham N., Khan L.A., Hall D.H., Fleming J.T., Gobel V.
Nat. Cell Biol. 13:1189-1201(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z81127 Genomic DNA. Translation: CAB03390.2.
Z81127 Genomic DNA. Translation: CCG28222.1.
PIRT25126.
RefSeqNP_001256547.1. NM_001269618.1. [Q9XVI6-2]
NP_001256548.1. NM_001269619.1. [Q9XVI6-1]
UniGeneCel.17398.

3D structure databases

ProteinModelPortalQ9XVI6.
SMRQ9XVI6. Positions 133-502.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-26788N.
IntActQ9XVI6. 1 interaction.
MINTMINT-1127619.
STRING6239.T22G5.5.1.

Proteomic databases

PaxDbQ9XVI6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT22G5.5a.1; T22G5.5a.1; WBGene00011932. [Q9XVI6-1]
T22G5.5a.2; T22G5.5a.2; WBGene00011932. [Q9XVI6-1]
GeneID179884.
KEGGcel:CELE_T22G5.5.
UCSCT22G5.5.1. c. elegans. [Q9XVI6-1]

Organism-specific databases

CTD179884.
WormBaseT22G5.5a; CE31996; WBGene00011932; sptl-3.
T22G5.5b; CE47328; WBGene00011932; sptl-3.

Phylogenomic databases

eggNOGCOG0156.
GeneTreeENSGT00550000074678.
HOGENOMHOG000206826.
InParanoidQ9XVI6.
OMAGINCGGS.
PhylomeDBQ9XVI6.

Enzyme and pathway databases

UniPathwayUPA00222.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio907264.
PROQ9XVI6.

Entry information

Entry nameSPTC3_CAEEL
AccessionPrimary (citable) accession number: Q9XVI6
Secondary accession number(s): H9G307
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase