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Q9XTY6

- NUMB1_CAEEL

UniProt

Q9XTY6 - NUMB1_CAEEL

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Protein
Numb-related protein 1
Gene
num-1, cka-1, T03D8.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the tethering and targeting of pkc-3 to modulate the intracellular distribution of the kinase. The complex formed with pkc-3 complexes are likely to be involved in assembly, maintenance, and/or regulation of protein complexes that execute asymmetric and/or polarized cell functions.1 Publication

GO - Molecular functioni

  1. ATPase binding Source: WormBase
  2. protein binding Source: IntAct
  3. protein domain specific binding Source: UniProtKB
  4. protein kinase C binding Source: UniProtKB
  5. transmembrane receptor protein tyrosine kinase adaptor activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. endocytic recycling Source: WormBase
  2. multicellular organismal development Source: UniProtKB-KW
  3. phospholipid transport Source: WormBase
  4. protein targeting to membrane Source: UniProtKB
  5. transmembrane receptor protein tyrosine kinase signaling pathway Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Numb-related protein 1
Alternative name(s):
CKA1
Protein kinase C adapter 1
Gene namesi
Name:num-1
Synonyms:cka-1
ORF Names:T03D8.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiT03D8.1a; CE18918; WBGene00003830; num-1.
T03D8.1b; CE18919; WBGene00003830; num-1.
T03D8.1c; CE30563; WBGene00003830; num-1.

Subcellular locationi

Cytoplasmcell cortex. Cytoplasmcytoskeleton. Membrane; Peripheral membrane protein
Note: Expressed at the inner surface of the plasma membrane at the cell periphery (which includes a region corresponding to plasma membrane and/or actin cortical cytoskeleton) in early embryos. Tightly associated with organelles and/or cytoskeletal structures with some diffuse expression in the cytoplasm. Differentially routed to lateral junctions between polarized cells.2 Publications

GO - Cellular componenti

  1. basolateral plasma membrane Source: WormBase
  2. cell cortex Source: UniProtKB-SubCell
  3. cytoplasm Source: UniProtKB
  4. cytoskeleton Source: UniProtKB-SubCell
  5. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi17 – 171S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-65. 1 Publication
Mutagenesisi17 – 171S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-65. 1 Publication
Mutagenesisi65 – 651S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-17. 1 Publication
Mutagenesisi65 – 651S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-17. 1 Publication
Mutagenesisi175 – 1751F → L: Prevents binding to pkc-3. 1 Publication
Mutagenesisi221 – 2211F → L: Prevents binding to pkc-3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Numb-related protein 11 Publication
PRO_0000021867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171Phosphoserine; by PKC1 Publication
Modified residuei65 – 651Phosphoserine; by PKC1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9XTY6.
PRIDEiQ9XTY6.

Expressioni

Tissue specificityi

Expressed in cells comprising the intestine, pharyngeal cells, the anal sphincter and depressor muscles.1 Publication

Developmental stagei

Expressed at each stage of development with predominance of isoform c in early larvae and isoform a in adults.1 Publication

Interactioni

Subunit structurei

Interacts with pkc-3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itsn-1Q9U2T93EBI-495781,EBI-2414252
pkc-3Q192665EBI-495798,EBI-319158

Protein-protein interaction databases

IntActiQ9XTY6. 3 interactions.
MINTiMINT-155059.

Structurei

3D structure databases

ProteinModelPortaliQ9XTY6.
SMRiQ9XTY6. Positions 93-227.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 255154PID
Add
BLAST

Domaini

The PID domain (phosphotyrosine interaction domain) of isoform a and isoform c is capable of binding residues 212-224 of pkc-3.

Sequence similaritiesi

Contains 1 PID domain.

Phylogenomic databases

eggNOGiNOG331167.
GeneTreeiENSGT00530000062937.
HOGENOMiHOG000017826.
KOiK06057.
OMAiACERASY.
PhylomeDBiQ9XTY6.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016698. Numb/numb-like.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamiPF00640. PID. 1 hit.
[Graphical view]
PIRSFiPIRSF017607. Numb/numb-like. 1 hit.
SMARTiSM00462. PTB. 1 hit.
[Graphical view]
PROSITEiPS01179. PID. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. Align

Isoform a1 Publication (identifier: Q9XTY6-1) [UniParc]FASTAAdd to Basket

Also known as: cka1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSASQGNVFT RGLSRISRRK KKTKSIQNSL VSEQQPSFDA AIVPMPIPND    50
KSSIFSKGMD RLRRSLRLPK KRRDRSHDRH LSPDVTGGSK TEQWQPDEGA 100
VRTGTCCFNV KYLGSVEVYE SRGMQVCEGA LKSLKASRRK PVKAVLYVSG 150
DGLRVVDQGN SRGLLVDQTI EKVSFCAPDR QTDKGFAYIC RDGASRRWMC 200
HGFLATKETG ERLSHAVGCA FSICLEKKKR RDEETAQVNV QSAQESTSST 250
PPKDIFHPNW EDNTSEGTST QNPSNSRSNL AYQSFRKHVS IEDRYLDPQS 300
VIINEVPASN HMDEIRRISK PRPTGNPALF LRQGSLRAPP DMPSSSDQFK 350
RNMSLRTVSN NPTERSPEKK SFGTQLYNEP IYEGDEDPLG LGITPPVVTK 400
TSGSLSNNGL DGINLNWKSI PAPVHQMQQH NANGDFVAAW PQNTIEKPTV 450
GPLDKLQKQF EDIKLISISS GENTPTTRSK ADEWLDDVLR VSMSMSPTSP 500
SSDPPSTSSY SVLPKSGPPP AHAPPPLPVR QAVSNGSPSI YQQQLQQANS 550
TRNSPAGINW NSSPNPMKIS QPPAKPVDPF DVQWSRLAVN NTH 593
Length:593
Mass (Da):65,510
Last modified:November 1, 1999 - v1
Checksum:iA3B0FE9C821D27D4
GO
Isoform b1 Publication (identifier: Q9XTY6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.
     193-370: GASRRWMCHG...NPTERSPEKK → MVLIDTEYVR...HLETRRSSCP

Note: Produced by alternative splicing. No experimental confirmation available.

Show »
Length:401
Mass (Da):44,265
Checksum:iFDCFB67BD4F91E2A
GO
Isoform c (identifier: Q9XTY6-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Note: Produced by alternative initiation at Met-45 of isoform a.

Show »
Length:549
Mass (Da):60,679
Checksum:iA126F40585F20FF5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 192192Missing in isoform b. 1 Publication
VSP_051598Add
BLAST
Alternative sequencei1 – 4444Missing in isoform c.
VSP_018786Add
BLAST
Alternative sequencei193 – 370178GASRR…SPEKK → MVLIDTEYVRAVVHNVGHRA RCGVASKVRALKLAHSQARL RSYSQACERASYIDGRCEYY PSLDVASSSRNSEMLNSGFF DGYSWNTPNIQSQSSSDVTA KTELRRLMTETSEDPIHKED SETLRRLIMWQEFRDAGVDV NTTQPGYGYGIEAKVEPFPQ KLQNYESIHLETRRSSCP in isoform b. 1 Publication
VSP_051599Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF286205 mRNA. Translation: AAK28740.1.
AF286206 mRNA. Translation: AAK28741.1.
Z92838 Genomic DNA. Translation: CAB07407.1.
Z92838 Genomic DNA. Translation: CAB07405.1.
Z92838 Genomic DNA. Translation: CAD30449.1.
PIRiT24379.
T24381.
RefSeqiNP_001024097.1. NM_001028926.2. [Q9XTY6-3]
NP_001024098.1. NM_001028927.1. [Q9XTY6-4]
NP_508021.1. NM_075620.5. [Q9XTY6-1]
UniGeneiCel.2522.

Genome annotation databases

EnsemblMetazoaiT03D8.1a; T03D8.1a; WBGene00003830. [Q9XTY6-1]
GeneIDi180367.
KEGGicel:CELE_T03D8.1.
UCSCiC28D4.2. c. elegans. [Q9XTY6-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF286205 mRNA. Translation: AAK28740.1 .
AF286206 mRNA. Translation: AAK28741.1 .
Z92838 Genomic DNA. Translation: CAB07407.1 .
Z92838 Genomic DNA. Translation: CAB07405.1 .
Z92838 Genomic DNA. Translation: CAD30449.1 .
PIRi T24379.
T24381.
RefSeqi NP_001024097.1. NM_001028926.2. [Q9XTY6-3 ]
NP_001024098.1. NM_001028927.1. [Q9XTY6-4 ]
NP_508021.1. NM_075620.5. [Q9XTY6-1 ]
UniGenei Cel.2522.

3D structure databases

ProteinModelPortali Q9XTY6.
SMRi Q9XTY6. Positions 93-227.
ModBasei Search...

Protein-protein interaction databases

IntActi Q9XTY6. 3 interactions.
MINTi MINT-155059.

Proteomic databases

PaxDbi Q9XTY6.
PRIDEi Q9XTY6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T03D8.1a ; T03D8.1a ; WBGene00003830 . [Q9XTY6-1 ]
GeneIDi 180367.
KEGGi cel:CELE_T03D8.1.
UCSCi C28D4.2. c. elegans. [Q9XTY6-1 ]

Organism-specific databases

CTDi 180367.
WormBasei T03D8.1a ; CE18918 ; WBGene00003830 ; num-1.
T03D8.1b ; CE18919 ; WBGene00003830 ; num-1.
T03D8.1c ; CE30563 ; WBGene00003830 ; num-1.

Phylogenomic databases

eggNOGi NOG331167.
GeneTreei ENSGT00530000062937.
HOGENOMi HOG000017826.
KOi K06057.
OMAi ACERASY.
PhylomeDBi Q9XTY6.

Miscellaneous databases

NextBioi 909060.
PROi Q9XTY6.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR016698. Numb/numb-like.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view ]
Pfami PF00640. PID. 1 hit.
[Graphical view ]
PIRSFi PIRSF017607. Numb/numb-like. 1 hit.
SMARTi SM00462. PTB. 1 hit.
[Graphical view ]
PROSITEi PS01179. PID. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C: characterization of Caenorhabditis elegans C kinase adapter 1, a protein that avidly binds protein kinase C3."
    Zhang L., Wu S.-L., Rubin C.S.
    J. Biol. Chem. 276:10463-10475(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), FUNCTION, INTERACTION WITH PKC-3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-175 AND PHE-221.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Structural properties and mechanisms that govern association of C kinase adapter 1 with protein kinase C3 and the cell periphery."
    Zhang L., Wu S.-L., Rubin C.S.
    J. Biol. Chem. 276:10476-10484(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PKC-3, PHOSPHORYLATION AT SER-17 AND SER-65, MUTAGENESIS OF SER-17 AND SER-65.

Entry informationi

Entry nameiNUMB1_CAEEL
AccessioniPrimary (citable) accession number: Q9XTY6
Secondary accession number(s): Q9BKB5, Q9XTY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi