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Q9XTY6

- NUMB1_CAEEL

UniProt

Q9XTY6 - NUMB1_CAEEL

Protein

Numb-related protein 1

Gene

num-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Involved in the tethering and targeting of pkc-3 to modulate the intracellular distribution of the kinase. The complex formed with pkc-3 complexes are likely to be involved in assembly, maintenance, and/or regulation of protein complexes that execute asymmetric and/or polarized cell functions.1 Publication

    GO - Molecular functioni

    1. ATPase binding Source: WormBase
    2. protein binding Source: IntAct
    3. protein domain specific binding Source: UniProtKB
    4. protein kinase C binding Source: UniProtKB
    5. transmembrane receptor protein tyrosine kinase adaptor activity Source: UniProtKB

    GO - Biological processi

    1. endocytic recycling Source: WormBase
    2. multicellular organismal development Source: UniProtKB-KW
    3. phospholipid transport Source: WormBase
    4. protein targeting to membrane Source: UniProtKB
    5. transmembrane receptor protein tyrosine kinase signaling pathway Source: GOC

    Keywords - Molecular functioni

    Developmental protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Numb-related protein 1
    Alternative name(s):
    CKA1
    Protein kinase C adapter 1
    Gene namesi
    Name:num-1Imported
    Synonyms:cka-1
    ORF Names:T03D8.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiT03D8.1a; CE18918; WBGene00003830; num-1.
    T03D8.1b; CE18919; WBGene00003830; num-1.
    T03D8.1c; CE30563; WBGene00003830; num-1.

    Subcellular locationi

    Cytoplasmcell cortex. Cytoplasmcytoskeleton. Membrane; Peripheral membrane protein
    Note: Expressed at the inner surface of the plasma membrane at the cell periphery (which includes a region corresponding to plasma membrane and/or actin cortical cytoskeleton) in early embryos. Tightly associated with organelles and/or cytoskeletal structures with some diffuse expression in the cytoplasm. Differentially routed to lateral junctions between polarized cells.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: WormBase
    2. cell cortex Source: UniProtKB-SubCell
    3. cytoplasm Source: UniProtKB
    4. cytoskeleton Source: UniProtKB-SubCell
    5. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi17 – 171S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-65. 1 Publication
    Mutagenesisi17 – 171S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-65. 1 Publication
    Mutagenesisi65 – 651S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-17. 1 Publication
    Mutagenesisi65 – 651S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-17. 1 Publication
    Mutagenesisi175 – 1751F → L: Prevents binding to pkc-3. 1 Publication
    Mutagenesisi221 – 2211F → L: Prevents binding to pkc-3. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 593593Numb-related protein 1PRO_0000021867Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei17 – 171Phosphoserine; by PKC1 Publication
    Modified residuei65 – 651Phosphoserine; by PKC1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9XTY6.
    PRIDEiQ9XTY6.

    Expressioni

    Tissue specificityi

    Expressed in cells comprising the intestine, pharyngeal cells, the anal sphincter and depressor muscles.1 Publication

    Developmental stagei

    Expressed at each stage of development with predominance of isoform c in early larvae and isoform a in adults.1 Publication

    Interactioni

    Subunit structurei

    Interacts with pkc-3.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itsn-1Q9U2T93EBI-495781,EBI-2414252
    pkc-3Q192665EBI-495781,EBI-319158

    Protein-protein interaction databases

    BioGridi45323. 1 interaction.
    IntActiQ9XTY6. 3 interactions.
    MINTiMINT-155059.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XTY6.
    SMRiQ9XTY6. Positions 93-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini102 – 255154PIDPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The PID domain (phosphotyrosine interaction domain) of isoform a and isoform c is capable of binding residues 212-224 of pkc-3.

    Sequence similaritiesi

    Contains 1 PID domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG331167.
    GeneTreeiENSGT00530000062937.
    HOGENOMiHOG000017826.
    KOiK06057.
    OMAiACERASY.
    PhylomeDBiQ9XTY6.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR016698. Numb/numb-like.
    IPR011993. PH_like_dom.
    IPR006020. PTB/PI_dom.
    [Graphical view]
    PfamiPF00640. PID. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017607. Numb/numb-like. 1 hit.
    SMARTiSM00462. PTB. 1 hit.
    [Graphical view]
    PROSITEiPS01179. PID. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. Align

    Isoform a1 Publication (identifier: Q9XTY6-1) [UniParc]FASTAAdd to Basket

    Also known as: cka11 Publication

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSASQGNVFT RGLSRISRRK KKTKSIQNSL VSEQQPSFDA AIVPMPIPND    50
    KSSIFSKGMD RLRRSLRLPK KRRDRSHDRH LSPDVTGGSK TEQWQPDEGA 100
    VRTGTCCFNV KYLGSVEVYE SRGMQVCEGA LKSLKASRRK PVKAVLYVSG 150
    DGLRVVDQGN SRGLLVDQTI EKVSFCAPDR QTDKGFAYIC RDGASRRWMC 200
    HGFLATKETG ERLSHAVGCA FSICLEKKKR RDEETAQVNV QSAQESTSST 250
    PPKDIFHPNW EDNTSEGTST QNPSNSRSNL AYQSFRKHVS IEDRYLDPQS 300
    VIINEVPASN HMDEIRRISK PRPTGNPALF LRQGSLRAPP DMPSSSDQFK 350
    RNMSLRTVSN NPTERSPEKK SFGTQLYNEP IYEGDEDPLG LGITPPVVTK 400
    TSGSLSNNGL DGINLNWKSI PAPVHQMQQH NANGDFVAAW PQNTIEKPTV 450
    GPLDKLQKQF EDIKLISISS GENTPTTRSK ADEWLDDVLR VSMSMSPTSP 500
    SSDPPSTSSY SVLPKSGPPP AHAPPPLPVR QAVSNGSPSI YQQQLQQANS 550
    TRNSPAGINW NSSPNPMKIS QPPAKPVDPF DVQWSRLAVN NTH 593
    Length:593
    Mass (Da):65,510
    Last modified:November 1, 1999 - v1
    Checksum:iA3B0FE9C821D27D4
    GO
    Isoform b (identifier: Q9XTY6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-192: Missing.
         193-370: GASRRWMCHG...NPTERSPEKK → MVLIDTEYVR...HLETRRSSCP

    Note: Produced by alternative splicing. No experimental confirmation available.

    Show »
    Length:401
    Mass (Da):44,265
    Checksum:iFDCFB67BD4F91E2A
    GO
    Isoform c (identifier: Q9XTY6-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-44: Missing.

    Note: Produced by alternative initiation at Met-45 of isoform a.

    Show »
    Length:549
    Mass (Da):60,679
    Checksum:iA126F40585F20FF5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 192192Missing in isoform b. CuratedVSP_051598Add
    BLAST
    Alternative sequencei1 – 4444Missing in isoform c. 1 PublicationVSP_018786Add
    BLAST
    Alternative sequencei193 – 370178GASRR…SPEKK → MVLIDTEYVRAVVHNVGHRA RCGVASKVRALKLAHSQARL RSYSQACERASYIDGRCEYY PSLDVASSSRNSEMLNSGFF DGYSWNTPNIQSQSSSDVTA KTELRRLMTETSEDPIHKED SETLRRLIMWQEFRDAGVDV NTTQPGYGYGIEAKVEPFPQ KLQNYESIHLETRRSSCP in isoform b. CuratedVSP_051599Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF286205 mRNA. Translation: AAK28740.1.
    AF286206 mRNA. Translation: AAK28741.1.
    Z92838 Genomic DNA. Translation: CAB07407.1.
    Z92838 Genomic DNA. Translation: CAB07405.1.
    Z92838 Genomic DNA. Translation: CAD30449.1.
    PIRiT24379.
    T24381.
    RefSeqiNP_001024097.1. NM_001028926.2. [Q9XTY6-3]
    NP_001024098.1. NM_001028927.1. [Q9XTY6-4]
    NP_508021.1. NM_075620.5. [Q9XTY6-1]
    UniGeneiCel.2522.

    Genome annotation databases

    EnsemblMetazoaiT03D8.1a; T03D8.1a; WBGene00003830. [Q9XTY6-1]
    GeneIDi180367.
    KEGGicel:CELE_T03D8.1.
    UCSCiC28D4.2. c. elegans. [Q9XTY6-1]

    Keywords - Coding sequence diversityi

    Alternative initiation, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF286205 mRNA. Translation: AAK28740.1 .
    AF286206 mRNA. Translation: AAK28741.1 .
    Z92838 Genomic DNA. Translation: CAB07407.1 .
    Z92838 Genomic DNA. Translation: CAB07405.1 .
    Z92838 Genomic DNA. Translation: CAD30449.1 .
    PIRi T24379.
    T24381.
    RefSeqi NP_001024097.1. NM_001028926.2. [Q9XTY6-3 ]
    NP_001024098.1. NM_001028927.1. [Q9XTY6-4 ]
    NP_508021.1. NM_075620.5. [Q9XTY6-1 ]
    UniGenei Cel.2522.

    3D structure databases

    ProteinModelPortali Q9XTY6.
    SMRi Q9XTY6. Positions 93-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 45323. 1 interaction.
    IntActi Q9XTY6. 3 interactions.
    MINTi MINT-155059.

    Proteomic databases

    PaxDbi Q9XTY6.
    PRIDEi Q9XTY6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T03D8.1a ; T03D8.1a ; WBGene00003830 . [Q9XTY6-1 ]
    GeneIDi 180367.
    KEGGi cel:CELE_T03D8.1.
    UCSCi C28D4.2. c. elegans. [Q9XTY6-1 ]

    Organism-specific databases

    CTDi 180367.
    WormBasei T03D8.1a ; CE18918 ; WBGene00003830 ; num-1.
    T03D8.1b ; CE18919 ; WBGene00003830 ; num-1.
    T03D8.1c ; CE30563 ; WBGene00003830 ; num-1.

    Phylogenomic databases

    eggNOGi NOG331167.
    GeneTreei ENSGT00530000062937.
    HOGENOMi HOG000017826.
    KOi K06057.
    OMAi ACERASY.
    PhylomeDBi Q9XTY6.

    Miscellaneous databases

    NextBioi 909060.
    PROi Q9XTY6.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR016698. Numb/numb-like.
    IPR011993. PH_like_dom.
    IPR006020. PTB/PI_dom.
    [Graphical view ]
    Pfami PF00640. PID. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017607. Numb/numb-like. 1 hit.
    SMARTi SM00462. PTB. 1 hit.
    [Graphical view ]
    PROSITEi PS01179. PID. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C: characterization of Caenorhabditis elegans C kinase adapter 1, a protein that avidly binds protein kinase C3."
      Zhang L., Wu S.-L., Rubin C.S.
      J. Biol. Chem. 276:10463-10475(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), FUNCTION, INTERACTION WITH PKC-3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-175 AND PHE-221.
      Strain: Bristol N21 Publication.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "Structural properties and mechanisms that govern association of C kinase adapter 1 with protein kinase C3 and the cell periphery."
      Zhang L., Wu S.-L., Rubin C.S.
      J. Biol. Chem. 276:10476-10484(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PKC-3, PHOSPHORYLATION AT SER-17 AND SER-65, MUTAGENESIS OF SER-17 AND SER-65.

    Entry informationi

    Entry nameiNUMB1_CAEEL
    AccessioniPrimary (citable) accession number: Q9XTY6
    Secondary accession number(s): Q9BKB5, Q9XTY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3