Q9XTY6 (NUMB1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Numb-related protein 1

Alternative name(s):
CKA1
Protein kinase C adapter 1

Gene names

Name:	num-1
Synonyms:	cka-1
ORF Names:	T03D8.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the tethering and targeting of pkc-3 to modulate the intracellular distribution of the kinase. The complex formed with pkc-3 complexes are likely to be involved in assembly, maintenance, and/or regulation of protein complexes that execute asymmetric and/or polarized cell functions. Ref.1
Subunit structure	Interacts with pkc-3. Ref.1 Ref.3
Subcellular location	Cytoplasm › cell cortex. Cytoplasm › cytoskeleton. Membrane; Peripheral membrane protein. Note: Expressed at the inner surface of the plasma membrane at the cell periphery (which includes a region corresponding to plasma membrane and/or actin cortical cytoskeleton) in early embryos. Tightly associated with organelles and/or cytoskeletal structures with some diffuse expression in the cytoplasm. Differentially routed to lateral junctions between polarized cells. Ref.1 Ref.3
Tissue specificity	Expressed in cells comprising the intestine, pharyngeal cells, the anal sphincter and depressor muscles. Ref.1
Developmental stage	Expressed at each stage of development with predominance of isoform c in early larvae and isoform a in adults. Ref.1
Domain	The PID domain (phosphotyrosine interaction domain) of isoform a and isoform c is capable of binding residues 212-224 of pkc-3.
Sequence similarities	Contains 1 PID domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative initiation Alternative splicing
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytic recycling Inferred from mutant phenotype PubMed 18493060. Source: WormBase multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW phospholipid transport Inferred from mutant phenotype PubMed 21917090. Source: WormBase protein targeting to membrane Inferred from direct assay Ref.1. Source: UniProtKB transmembrane receptor protein tyrosine kinase signaling pathway Inferred from physical interaction Ref.1. Source: GOC
Cellular_component	basolateral plasma membrane Inferred from direct assay PubMed 18493060. Source: WormBase cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay Ref.3. Source: UniProtKB cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itsn-1	Q9U2T9	3	EBI-495781,EBI-2414252
pkc-3	Q19266	5	EBI-495798,EBI-319158

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform a Ref.1 (identifier: Q9XTY6-1)

Also known as: cka1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b Ref.2 (identifier: Q9XTY6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.
     193-370: GASRRWMCHG...NPTERSPEKK → MVLIDTEYVR...HLETRRSSCP

Note: Produced by alternative splicing. No experimental confirmation available.

Isoform c (identifier: Q9XTY6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Note: Produced by alternative initiation at Met-45 of isoform a.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Numb-related protein 1 Ref.1		PRO_0000021867
Regions
	Domain	102 – 255	154	PID
Amino acid modifications
	Modified residue	17	1	Phosphoserine; by PKC Ref.3
	Modified residue	65	1	Phosphoserine; by PKC Ref.3
Natural variations
	Alternative sequence	1 – 192	192	Missing in isoform b. Ref.2		VSP_051598
	Alternative sequence	1 – 44	44	Missing in isoform c.		VSP_018786
	Alternative sequence	193 – 370	178	GASRR…SPEKK → MVLIDTEYVRAVVHNVGHRA RCGVASKVRALKLAHSQARL RSYSQACERASYIDGRCEYY PSLDVASSSRNSEMLNSGFF DGYSWNTPNIQSQSSSDVTA KTELRRLMTETSEDPIHKED SETLRRLIMWQEFRDAGVDV NTTQPGYGYGIEAKVEPFPQ KLQNYESIHLETRRSSCP in isoform b. Ref.2		VSP_051599
Experimental info
	Mutagenesis	17	1	S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-65. Ref.3
	Mutagenesis	17	1	S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-65. Ref.3
	Mutagenesis	65	1	S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-17. Ref.3
	Mutagenesis	65	1	S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-17. Ref.3
	Mutagenesis	175	1	F → L: Prevents binding to pkc-3. Ref.1
	Mutagenesis	221	1	F → L: Prevents binding to pkc-3. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform a (cka1) [UniParc]. Last modified November 1, 1999. Version 1. Checksum: A3B0FE9C821D27D4	FASTA	593	65,510
	10 20 30 40 50 60 MSASQGNVFT RGLSRISRRK KKTKSIQNSL VSEQQPSFDA AIVPMPIPND KSSIFSKGMD 70 80 90 100 110 120 RLRRSLRLPK KRRDRSHDRH LSPDVTGGSK TEQWQPDEGA VRTGTCCFNV KYLGSVEVYE 130 140 150 160 170 180 SRGMQVCEGA LKSLKASRRK PVKAVLYVSG DGLRVVDQGN SRGLLVDQTI EKVSFCAPDR 190 200 210 220 230 240 QTDKGFAYIC RDGASRRWMC HGFLATKETG ERLSHAVGCA FSICLEKKKR RDEETAQVNV 250 260 270 280 290 300 QSAQESTSST PPKDIFHPNW EDNTSEGTST QNPSNSRSNL AYQSFRKHVS IEDRYLDPQS 310 320 330 340 350 360 VIINEVPASN HMDEIRRISK PRPTGNPALF LRQGSLRAPP DMPSSSDQFK RNMSLRTVSN 370 380 390 400 410 420 NPTERSPEKK SFGTQLYNEP IYEGDEDPLG LGITPPVVTK TSGSLSNNGL DGINLNWKSI 430 440 450 460 470 480 PAPVHQMQQH NANGDFVAAW PQNTIEKPTV GPLDKLQKQF EDIKLISISS GENTPTTRSK 490 500 510 520 530 540 ADEWLDDVLR VSMSMSPTSP SSDPPSTSSY SVLPKSGPPP AHAPPPLPVR QAVSNGSPSI 550 560 570 580 590 YQQQLQQANS TRNSPAGINW NSSPNPMKIS QPPAKPVDPF DVQWSRLAVN NTH
	Isoform b [UniParc]. Checksum: FDCFB67BD4F91E2A	FASTA	401	44,265
	10 20 30 40 50 60 MVLIDTEYVR AVVHNVGHRA RCGVASKVRA LKLAHSQARL RSYSQACERA SYIDGRCEYY 70 80 90 100 110 120 PSLDVASSSR NSEMLNSGFF DGYSWNTPNI QSQSSSDVTA KTELRRLMTE TSEDPIHKED 130 140 150 160 170 180 SETLRRLIMW QEFRDAGVDV NTTQPGYGYG IEAKVEPFPQ KLQNYESIHL ETRRSSCPSF 190 200 210 220 230 240 GTQLYNEPIY EGDEDPLGLG ITPPVVTKTS GSLSNNGLDG INLNWKSIPA PVHQMQQHNA 250 260 270 280 290 300 NGDFVAAWPQ NTIEKPTVGP LDKLQKQFED IKLISISSGE NTPTTRSKAD EWLDDVLRVS 310 320 330 340 350 360 MSMSPTSPSS DPPSTSSYSV LPKSGPPPAH APPPLPVRQA VSNGSPSIYQ QQLQQANSTR 370 380 390 400 NSPAGINWNS SPNPMKISQP PAKPVDPFDV QWSRLAVNNT H
	Isoform c [UniParc]. Checksum: A126F40585F20FF5	FASTA	549	60,679
	10 20 30 40 50 60 MPIPNDKSSI FSKGMDRLRR SLRLPKKRRD RSHDRHLSPD VTGGSKTEQW QPDEGAVRTG 70 80 90 100 110 120 TCCFNVKYLG SVEVYESRGM QVCEGALKSL KASRRKPVKA VLYVSGDGLR VVDQGNSRGL 130 140 150 160 170 180 LVDQTIEKVS FCAPDRQTDK GFAYICRDGA SRRWMCHGFL ATKETGERLS HAVGCAFSIC 190 200 210 220 230 240 LEKKKRRDEE TAQVNVQSAQ ESTSSTPPKD IFHPNWEDNT SEGTSTQNPS NSRSNLAYQS 250 260 270 280 290 300 FRKHVSIEDR YLDPQSVIIN EVPASNHMDE IRRISKPRPT GNPALFLRQG SLRAPPDMPS 310 320 330 340 350 360 SSDQFKRNMS LRTVSNNPTE RSPEKKSFGT QLYNEPIYEG DEDPLGLGIT PPVVTKTSGS 370 380 390 400 410 420 LSNNGLDGIN LNWKSIPAPV HQMQQHNANG DFVAAWPQNT IEKPTVGPLD KLQKQFEDIK 430 440 450 460 470 480 LISISSGENT PTTRSKADEW LDDVLRVSMS MSPTSPSSDP PSTSSYSVLP KSGPPPAHAP 490 500 510 520 530 540 PPLPVRQAVS NGSPSIYQQQ LQQANSTRNS PAGINWNSSP NPMKISQPPA KPVDPFDVQW SRLAVNNTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C: characterization of Caenorhabditis elegans C kinase adapter 1, a protein that avidly binds protein kinase C3." Zhang L., Wu S.-L., Rubin C.S. J. Biol. Chem. 276:10463-10475(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), FUNCTION, INTERACTION WITH PKC-3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-175 AND PHE-221. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Structural properties and mechanisms that govern association of C kinase adapter 1 with protein kinase C3 and the cell periphery." Zhang L., Wu S.-L., Rubin C.S. J. Biol. Chem. 276:10476-10484(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PKC-3, PHOSPHORYLATION AT SER-17 AND SER-65, MUTAGENESIS OF SER-17 AND SER-65.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF286205 mRNA. Translation: AAK28740.1. AF286206 mRNA. Translation: AAK28741.1. Z92838 Genomic DNA. Translation: CAB07407.1. Z92838 Genomic DNA. Translation: CAB07405.1. Z92838 Genomic DNA. Translation: CAD30449.1.
PIR	T24379. T24381.
RefSeq	NP_001024097.1. NM_001028926.2. NP_001024098.1. NM_001028927.1. NP_508021.1. NM_075620.5.
UniGene	Cel.2522.
3D structure databases
ProteinModelPortal	Q9XTY6.
SMR	Q9XTY6. Positions 93-227.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9XTY6. 3 interactions.
MINT	MINT-155059.
Proteomic databases
PaxDb	Q9XTY6.
PRIDE	Q9XTY6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T03D8.1a; T03D8.1a; T03D8.1.
GeneID	180367.
KEGG	cel:CELE_T03D8.1.
UCSC	C28D4.2. c. elegans.
Organism-specific databases
CTD	180367.
WormBase	T03D8.1a; CE18918; WBGene00003830; num-1. T03D8.1b; CE18919; WBGene00003830; num-1. T03D8.1c; CE30563; WBGene00003830; num-1.
Phylogenomic databases
eggNOG	NOG331167.
GeneTree	ENSGT00530000062937.
HOGENOM	HOG000017826.
KO	K06057.
Gene expression databases
ArrayExpress	Q9XTY6.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR016698. Numb/numb-like. IPR011993. PH_like_dom. IPR006020. PTyr_interaction_dom. [Graphical view]
Pfam	PF00640. PID. 1 hit. [Graphical view]
PIRSF	PIRSF017607. Numb/numb-like. 1 hit.
SMART	SM00462. PTB. 1 hit. [Graphical view]
PROSITE	PS01179. PID. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	909060.

Entry information

Entry name

NUMB1_CAEEL

Accession

Primary (citable) accession number: Q9XTY6
Secondary accession number(s): Q9BKB5, Q9XTY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	November 1, 1999
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project