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Protein

Numb-related protein 1

Gene

num-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the tethering and targeting of pkc-3 to modulate the intracellular distribution of the kinase. The complex formed with pkc-3 complexes are likely to be involved in assembly, maintenance, and/or regulation of protein complexes that execute asymmetric and/or polarized cell functions.1 Publication

GO - Molecular functioni

  • ATPase binding Source: WormBase
  • protein domain specific binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • transmembrane receptor protein tyrosine kinase adaptor activity Source: UniProtKB

GO - Biological processi

  • endocytic recycling Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • phospholipid transport Source: WormBase
  • protein targeting to membrane Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Numb-related protein 1
Alternative name(s):
CKA1
Protein kinase C adapter 1
Gene namesi
Name:num-1Imported
Synonyms:cka-1
ORF Names:T03D8.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT03D8.1a ; CE18918 ; WBGene00003830 ; num-1
T03D8.1b ; CE18919 ; WBGene00003830 ; num-1
T03D8.1c ; CE30563 ; WBGene00003830 ; num-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-65. 1 Publication1
Mutagenesisi17S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-65. 1 Publication1
Mutagenesisi65S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-17. 1 Publication1
Mutagenesisi65S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-17. 1 Publication1
Mutagenesisi175F → L: Prevents binding to pkc-3. 1 Publication1
Mutagenesisi221F → L: Prevents binding to pkc-3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000218671 – 593Numb-related protein 1Add BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17Phosphoserine; by PKC1 Publication1
Modified residuei65Phosphoserine; by PKC1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9XTY6
PaxDbiQ9XTY6
PeptideAtlasiQ9XTY6
PRIDEiQ9XTY6

PTM databases

iPTMnetiQ9XTY6

Expressioni

Tissue specificityi

Expressed in cells comprising the intestine, pharyngeal cells, the anal sphincter and depressor muscles.1 Publication

Developmental stagei

Expressed at each stage of development with predominance of isoform c in early larvae and isoform a in adults.1 Publication

Gene expression databases

BgeeiWBGene00003830
ExpressionAtlasiQ9XTY6 baseline and differential

Interactioni

Subunit structurei

Interacts with pkc-3.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ATPase binding Source: WormBase
  • protein domain specific binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • transmembrane receptor protein tyrosine kinase adaptor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi45323, 1 interactor
IntActiQ9XTY6, 3 interactors
MINTiQ9XTY6
STRINGi6239.T03D8.1d

Structurei

3D structure databases

ProteinModelPortaliQ9XTY6
SMRiQ9XTY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 255PIDPROSITE-ProRule annotationAdd BLAST154

Domaini

The PID domain (phosphotyrosine interaction domain) of isoform a and isoform c is capable of binding residues 212-224 of pkc-3.

Phylogenomic databases

eggNOGiKOG3537 Eukaryota
ENOG410XT15 LUCA
GeneTreeiENSGT00530000062937
HOGENOMiHOG000017826
InParanoidiQ9XTY6
PhylomeDBiQ9XTY6

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR016698 Numb/numb-like
IPR011993 PH-like_dom_sf
IPR006020 PTB/PI_dom
PfamiView protein in Pfam
PF00640 PID, 1 hit
PIRSFiPIRSF017607 Numb/numb-like, 1 hit
SMARTiView protein in SMART
SM00462 PTB, 1 hit
PROSITEiView protein in PROSITE
PS01179 PID, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform a1 Publication (identifier: Q9XTY6-1) [UniParc]FASTAAdd to basket
Also known as: cka11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSASQGNVFT RGLSRISRRK KKTKSIQNSL VSEQQPSFDA AIVPMPIPND
60 70 80 90 100
KSSIFSKGMD RLRRSLRLPK KRRDRSHDRH LSPDVTGGSK TEQWQPDEGA
110 120 130 140 150
VRTGTCCFNV KYLGSVEVYE SRGMQVCEGA LKSLKASRRK PVKAVLYVSG
160 170 180 190 200
DGLRVVDQGN SRGLLVDQTI EKVSFCAPDR QTDKGFAYIC RDGASRRWMC
210 220 230 240 250
HGFLATKETG ERLSHAVGCA FSICLEKKKR RDEETAQVNV QSAQESTSST
260 270 280 290 300
PPKDIFHPNW EDNTSEGTST QNPSNSRSNL AYQSFRKHVS IEDRYLDPQS
310 320 330 340 350
VIINEVPASN HMDEIRRISK PRPTGNPALF LRQGSLRAPP DMPSSSDQFK
360 370 380 390 400
RNMSLRTVSN NPTERSPEKK SFGTQLYNEP IYEGDEDPLG LGITPPVVTK
410 420 430 440 450
TSGSLSNNGL DGINLNWKSI PAPVHQMQQH NANGDFVAAW PQNTIEKPTV
460 470 480 490 500
GPLDKLQKQF EDIKLISISS GENTPTTRSK ADEWLDDVLR VSMSMSPTSP
510 520 530 540 550
SSDPPSTSSY SVLPKSGPPP AHAPPPLPVR QAVSNGSPSI YQQQLQQANS
560 570 580 590
TRNSPAGINW NSSPNPMKIS QPPAKPVDPF DVQWSRLAVN NTH
Length:593
Mass (Da):65,510
Last modified:November 1, 1999 - v1
Checksum:iA3B0FE9C821D27D4
GO
Isoform b (identifier: Q9XTY6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.
     193-370: GASRRWMCHG...NPTERSPEKK → MVLIDTEYVR...HLETRRSSCP

Note: Produced by alternative splicing. No experimental confirmation available.
Show »
Length:401
Mass (Da):44,265
Checksum:iFDCFB67BD4F91E2A
GO
Isoform c (identifier: Q9XTY6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Note: Produced by alternative initiation at Met-45 of isoform a.
Show »
Length:549
Mass (Da):60,679
Checksum:iA126F40585F20FF5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0515981 – 192Missing in isoform b. CuratedAdd BLAST192
Alternative sequenceiVSP_0187861 – 44Missing in isoform c. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_051599193 – 370GASRR…SPEKK → MVLIDTEYVRAVVHNVGHRA RCGVASKVRALKLAHSQARL RSYSQACERASYIDGRCEYY PSLDVASSSRNSEMLNSGFF DGYSWNTPNIQSQSSSDVTA KTELRRLMTETSEDPIHKED SETLRRLIMWQEFRDAGVDV NTTQPGYGYGIEAKVEPFPQ KLQNYESIHLETRRSSCP in isoform b. CuratedAdd BLAST178

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286205 mRNA Translation: AAK28740.1
AF286206 mRNA Translation: AAK28741.1
Z92838 Genomic DNA Translation: CAB07407.1
Z92838 Genomic DNA Translation: CAB07405.1
Z92838 Genomic DNA Translation: CAD30449.1
PIRiT24379
T24381
RefSeqiNP_001024097.1, NM_001028926.2 [Q9XTY6-3]
NP_001024098.1, NM_001028927.1 [Q9XTY6-4]
NP_508021.1, NM_075620.5 [Q9XTY6-1]
UniGeneiCel.2522

Genome annotation databases

EnsemblMetazoaiT03D8.1a; T03D8.1a; WBGene00003830 [Q9XTY6-1]
GeneIDi180367
UCSCiC28D4.2 c. elegans [Q9XTY6-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUMB1_CAEEL
AccessioniPrimary (citable) accession number: Q9XTY6
Secondary accession number(s): Q9BKB5, Q9XTY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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