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Q9XTY6 (NUMB1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Numb-related protein 1
Alternative name(s):
CKA1
Protein kinase C adapter 1
Gene names
Name:num-1
Synonyms:cka-1
ORF Names:T03D8.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the tethering and targeting of pkc-3 to modulate the intracellular distribution of the kinase. The complex formed with pkc-3 complexes are likely to be involved in assembly, maintenance, and/or regulation of protein complexes that execute asymmetric and/or polarized cell functions. Ref.1

Subunit structure

Interacts with pkc-3. Ref.1 Ref.3

Subcellular location

Cytoplasmcell cortex. Cytoplasmcytoskeleton. Membrane; Peripheral membrane protein. Note: Expressed at the inner surface of the plasma membrane at the cell periphery (which includes a region corresponding to plasma membrane and/or actin cortical cytoskeleton) in early embryos. Tightly associated with organelles and/or cytoskeletal structures with some diffuse expression in the cytoplasm. Differentially routed to lateral junctions between polarized cells. Ref.1 Ref.3

Tissue specificity

Expressed in cells comprising the intestine, pharyngeal cells, the anal sphincter and depressor muscles. Ref.1

Developmental stage

Expressed at each stage of development with predominance of isoform c in early larvae and isoform a in adults. Ref.1

Domain

The PID domain (phosphotyrosine interaction domain) of isoform a and isoform c is capable of binding residues 212-224 of pkc-3.

Sequence similarities

Contains 1 PID domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Membrane
   Coding sequence diversityAlternative initiation
Alternative splicing
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendocytic recycling

Inferred from mutant phenotype PubMed 18493060. Source: WormBase

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

phospholipid transport

Inferred from mutant phenotype PubMed 21917090. Source: WormBase

protein targeting to membrane

Inferred from direct assay Ref.1. Source: UniProtKB

transmembrane receptor protein tyrosine kinase signaling pathway

Inferred from physical interaction Ref.1. Source: GOC

   Cellular_componentbasolateral plasma membrane

Inferred from direct assay PubMed 18493060. Source: WormBase

cell cortex

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay Ref.3. Source: UniProtKB

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of plasma membrane

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionATPase binding

Inferred from physical interaction PubMed 21917090. Source: WormBase

protein binding

Inferred from physical interaction Ref.1Ref.3PubMed 23549480. Source: IntAct

protein domain specific binding

Inferred from physical interaction Ref.1. Source: UniProtKB

protein kinase C binding

Inferred from physical interaction Ref.1. Source: UniProtKB

transmembrane receptor protein tyrosine kinase adaptor activity

Inferred from physical interaction Ref.1. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itsn-1Q9U2T93EBI-495781,EBI-2414252
pkc-3Q192665EBI-495781,EBI-319158

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]
Isoform a Ref.1 (identifier: Q9XTY6-1)

Also known as: cka1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b Ref.2 (identifier: Q9XTY6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.
     193-370: GASRRWMCHG...NPTERSPEKK → MVLIDTEYVR...HLETRRSSCP
Note: Produced by alternative splicing. No experimental confirmation available.
Isoform c (identifier: Q9XTY6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
Note: Produced by alternative initiation at Met-45 of isoform a.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 593593Numb-related protein 1 Ref.1
PRO_0000021867

Regions

Domain102 – 255154PID

Amino acid modifications

Modified residue171Phosphoserine; by PKC Ref.3
Modified residue651Phosphoserine; by PKC Ref.3

Natural variations

Alternative sequence1 – 192192Missing in isoform b. Ref.2
VSP_051598
Alternative sequence1 – 4444Missing in isoform c.
VSP_018786
Alternative sequence193 – 370178GASRR…SPEKK → MVLIDTEYVRAVVHNVGHRA RCGVASKVRALKLAHSQARL RSYSQACERASYIDGRCEYY PSLDVASSSRNSEMLNSGFF DGYSWNTPNIQSQSSSDVTA KTELRRLMTETSEDPIHKED SETLRRLIMWQEFRDAGVDV NTTQPGYGYGIEAKVEPFPQ KLQNYESIHLETRRSSCP in isoform b. Ref.2
VSP_051599

Experimental info

Mutagenesis171S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-65. Ref.3
Mutagenesis171S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-65. Ref.3
Mutagenesis651S → A: Has no effect on efficiently routing num-1 to the cell periphery; when associated with A-17. Ref.3
Mutagenesis651S → Q: Promotes accumulation of num-1 in the cytoplasm; when associated with Q-17. Ref.3
Mutagenesis1751F → L: Prevents binding to pkc-3. Ref.1
Mutagenesis2211F → L: Prevents binding to pkc-3. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform a (cka1) [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A3B0FE9C821D27D4

FASTA59365,510
        10         20         30         40         50         60 
MSASQGNVFT RGLSRISRRK KKTKSIQNSL VSEQQPSFDA AIVPMPIPND KSSIFSKGMD 

        70         80         90        100        110        120 
RLRRSLRLPK KRRDRSHDRH LSPDVTGGSK TEQWQPDEGA VRTGTCCFNV KYLGSVEVYE 

       130        140        150        160        170        180 
SRGMQVCEGA LKSLKASRRK PVKAVLYVSG DGLRVVDQGN SRGLLVDQTI EKVSFCAPDR 

       190        200        210        220        230        240 
QTDKGFAYIC RDGASRRWMC HGFLATKETG ERLSHAVGCA FSICLEKKKR RDEETAQVNV 

       250        260        270        280        290        300 
QSAQESTSST PPKDIFHPNW EDNTSEGTST QNPSNSRSNL AYQSFRKHVS IEDRYLDPQS 

       310        320        330        340        350        360 
VIINEVPASN HMDEIRRISK PRPTGNPALF LRQGSLRAPP DMPSSSDQFK RNMSLRTVSN 

       370        380        390        400        410        420 
NPTERSPEKK SFGTQLYNEP IYEGDEDPLG LGITPPVVTK TSGSLSNNGL DGINLNWKSI 

       430        440        450        460        470        480 
PAPVHQMQQH NANGDFVAAW PQNTIEKPTV GPLDKLQKQF EDIKLISISS GENTPTTRSK 

       490        500        510        520        530        540 
ADEWLDDVLR VSMSMSPTSP SSDPPSTSSY SVLPKSGPPP AHAPPPLPVR QAVSNGSPSI 

       550        560        570        580        590 
YQQQLQQANS TRNSPAGINW NSSPNPMKIS QPPAKPVDPF DVQWSRLAVN NTH 

« Hide

Isoform b [UniParc].

Checksum: FDCFB67BD4F91E2A
Show »

FASTA40144,265
Isoform c [UniParc].

Checksum: A126F40585F20FF5
Show »

FASTA54960,679

References

« Hide 'large scale' references
[1]"A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C: characterization of Caenorhabditis elegans C kinase adapter 1, a protein that avidly binds protein kinase C3."
Zhang L., Wu S.-L., Rubin C.S.
J. Biol. Chem. 276:10463-10475(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), FUNCTION, INTERACTION WITH PKC-3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-175 AND PHE-221.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Structural properties and mechanisms that govern association of C kinase adapter 1 with protein kinase C3 and the cell periphery."
Zhang L., Wu S.-L., Rubin C.S.
J. Biol. Chem. 276:10476-10484(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PKC-3, PHOSPHORYLATION AT SER-17 AND SER-65, MUTAGENESIS OF SER-17 AND SER-65.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF286205 mRNA. Translation: AAK28740.1.
AF286206 mRNA. Translation: AAK28741.1.
Z92838 Genomic DNA. Translation: CAB07407.1.
Z92838 Genomic DNA. Translation: CAB07405.1.
Z92838 Genomic DNA. Translation: CAD30449.1.
PIRT24379.
T24381.
RefSeqNP_001024097.1. NM_001028926.2. [Q9XTY6-3]
NP_001024098.1. NM_001028927.1. [Q9XTY6-4]
NP_508021.1. NM_075620.5. [Q9XTY6-1]
UniGeneCel.2522.

3D structure databases

ProteinModelPortalQ9XTY6.
SMRQ9XTY6. Positions 93-227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9XTY6. 3 interactions.
MINTMINT-155059.

Proteomic databases

PaxDbQ9XTY6.
PRIDEQ9XTY6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT03D8.1a; T03D8.1a; WBGene00003830. [Q9XTY6-1]
GeneID180367.
KEGGcel:CELE_T03D8.1.
UCSCC28D4.2. c. elegans. [Q9XTY6-1]

Organism-specific databases

CTD180367.
WormBaseT03D8.1a; CE18918; WBGene00003830; num-1.
T03D8.1b; CE18919; WBGene00003830; num-1.
T03D8.1c; CE30563; WBGene00003830; num-1.

Phylogenomic databases

eggNOGNOG331167.
GeneTreeENSGT00530000062937.
HOGENOMHOG000017826.
KOK06057.
OMAACERASY.
PhylomeDBQ9XTY6.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR016698. Numb/numb-like.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamPF00640. PID. 1 hit.
[Graphical view]
PIRSFPIRSF017607. Numb/numb-like. 1 hit.
SMARTSM00462. PTB. 1 hit.
[Graphical view]
PROSITEPS01179. PID. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio909060.
PROQ9XTY6.

Entry information

Entry nameNUMB1_CAEEL
AccessionPrimary (citable) accession number: Q9XTY6
Secondary accession number(s): Q9BKB5, Q9XTY4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1999
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase