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Protein

Insulin receptor substrate 1

Gene

chico

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). May mediate the control of various cellular processes by insulin-like peptides. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains. Involved in control of cell proliferation, cell size, and body and organ growth throughout development. Also has a role in a signaling pathway controlling the physiological response required to endure periods of low nutrient conditions. Insulin/insulin-like growth factor (IGF) signaling pathway has a role in regulating aging and is necessary in the ovary for vitellogenic maturation.By similarity4 Publications

Miscellaneous

'Chico' means 'small boy' in Spanish.Curated

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: FlyBase
  • insulin receptor binding Source: UniProtKB
  • phosphatidylinositol 3-kinase binding Source: UniProtKB
  • SH2 domain binding Source: UniProtKB
  • signal transducer activity Source: GO_Central

GO - Biological processi

  • aging Source: FlyBase
  • cell morphogenesis Source: FlyBase
  • cellular metabolic process Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • germ-line stem-cell niche homeostasis Source: FlyBase
  • growth of a germarium-derived egg chamber Source: FlyBase
  • insulin-like growth factor receptor signaling pathway Source: UniProtKB
  • insulin receptor signaling pathway Source: FlyBase
  • lipid metabolic process Source: FlyBase
  • long-term synaptic potentiation Source: FlyBase
  • male germ-line stem cell asymmetric division Source: FlyBase
  • multicellular organism aging Source: UniProtKB
  • multicellular organism growth Source: FlyBase
  • olfactory learning Source: FlyBase
  • phosphatidylinositol phosphorylation Source: Reactome
  • positive regulation of cell growth Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • positive regulation of immune response Source: FlyBase
  • positive regulation of multicellular organism growth Source: FlyBase
  • positive regulation of organ growth Source: FlyBase
  • primary spermatocyte growth Source: FlyBase
  • regulation of activin receptor signaling pathway Source: FlyBase
  • regulation of cell size Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of lipid metabolic process Source: GO_Central
  • regulation of multicellular organism growth Source: FlyBase
  • regulation of organ growth Source: FlyBase
  • regulation of tube length, open tracheal system Source: FlyBase
  • response to anoxia Source: FlyBase
  • response to starvation Source: FlyBase
  • vitellogenesis Source: UniProtKB

Keywordsi

Biological processDifferentiation, Growth regulation, Oogenesis

Enzyme and pathway databases

ReactomeiR-DME-109704. PI3K Cascade.
R-DME-110478. Insulin signaling pathway.
R-DME-112412. SOS-mediated signalling.
R-DME-1257604. PIP3 activates AKT signaling.
R-DME-1266695. Interleukin-7 signaling.
R-DME-198203. PI3K/AKT activation.
R-DME-5673001. RAF/MAP kinase cascade.
R-DME-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-DME-74749. Signal attenuation.
SignaLinkiQ9XTN2.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin receptor substrate 1
Short name:
dIRS
Alternative name(s):
Protein chico
Gene namesi
Name:chico
Synonyms:IRS
ORF Names:CG5686
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0024248. chico.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Female sterile. Extends fruit fly median life-span by up to 48% in homozygotes and 36% in heterozygotes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842351 – 968Insulin receptor substrate 1Add BLAST968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei286Phosphoserine1 Publication1
Modified residuei287Phosphoserine1 Publication1
Modified residuei342Phosphoserine1 Publication1
Modified residuei411Phosphotyrosine; by INSRBy similarity1
Modified residuei555Phosphoserine1 Publication1
Modified residuei911Phosphotyrosine; by INSRBy similarity1
Modified residuei932Phosphoserine1 Publication1
Modified residuei935Phosphoserine1 Publication1
Modified residuei948Phosphotyrosine; by INSRBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9XTN2.
PRIDEiQ9XTN2.

PTM databases

iPTMnetiQ9XTN2.

Expressioni

Developmental stagei

Expressed during G1, S and G2 phase of the cell cycle.1 Publication

Gene expression databases

BgeeiFBgn0024248.
ExpressionAtlasiQ9XTN2. differential.
GenevisibleiQ9XTN2. DM.

Interactioni

Subunit structurei

Bindings to phosphatidylinositol 3-kinase and SHP2.

Binary interactionsi

WithEntry#Exp.IntActNotes
InRP092083EBI-176370,EBI-92063

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: FlyBase
  • insulin receptor binding Source: UniProtKB
  • phosphatidylinositol 3-kinase binding Source: UniProtKB
  • SH2 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi72865. 37 interactors.
DIPiDIP-23631N.
IntActiQ9XTN2. 7 interactors.
MINTiMINT-936125.
STRINGi7227.FBpp0079677.

Structurei

3D structure databases

ProteinModelPortaliQ9XTN2.
SMRiQ9XTN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 109PHPROSITE-ProRule annotationCuratedAdd BLAST102
Domaini122 – 236IRS-type PTBPROSITE-ProRule annotationAdd BLAST115

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi411 – 414YXXM motif 14
Motifi641 – 644YXXM motif 24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi855 – 858Poly-Ser4
Compositional biasi870 – 873Poly-Ala4
Compositional biasi956 – 963Poly-Ser8

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IXEK. Eukaryota.
ENOG410Z9EP. LUCA.
GeneTreeiENSGT00530000063420.
InParanoidiQ9XTN2.
KOiK16172.
OMAiSKIPRCD.
OrthoDBiEOG091G02EF.
PhylomeDBiQ9XTN2.

Family and domain databases

CDDicd01204. PTB_IRS. 1 hit.
Gene3Di2.30.29.30. 2 hits.
InterProiView protein in InterPro
IPR002404. IRS_PTB.
IPR011993. PH-like_dom_sf.
IPR001849. PH_domain.
PfamiView protein in Pfam
PF02174. IRS. 1 hit.
PRINTSiPR00628. INSULINRSI.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiView protein in PROSITE
PS51064. IRS_PTB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XTN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASISDDGMA LSGYLKKLKT MKKKFFVLYE ETSTSAARLE YYDTEKKFLQ
60 70 80 90 100
RAEPKRVIYL KNCFNINRRL DTKHRFVIVL SSRDGGFGIV LENENDLRKW
110 120 130 140 150
LDKLLVLQRN IANSNGTAHS PYDHVWQVVI QKKGISEKVG ITGTYHCCLT
160 170 180 190 200
SKSLTFVCIG PEKTPNGEDR VASIEILLTT IRRCGHASPQ CIFYVELGRQ
210 220 230 240 250
SVLGSGDLWM ETDNAAIATN MHNTILSAMS AKTESNTNLI NVYQNRPDLS
260 270 280 290 300
HEPMRKRSSS ANEASKPINV NVIQNSQNSL ELRSCSSPHN YGFGRERCDS
310 320 330 340 350
LPTRNGTLSE SSNQTYFGSN HGLRSNTISG IRPHSTNKHS NSPTFTMPLR
360 370 380 390 400
CSESEESSIS VDESDDNGSF SHYRLNTRSS ETAIPEENID DFASAELFSK
410 420 430 440 450
VTEQNVSDEN YIPMNPVNPT DAIHEKEKAD MQRLEDASLH FNFPEHASEK
460 470 480 490 500
LAKDFDLDSD NQCCRPIRAY SIGNKVEHLK FNKRLGHLND TGQNPNRVRA
510 520 530 540 550
YSVGSKSKIP RCDLQRVVLV EDNKHEFTAN RSQSSITKEG TSYGSSANRQ
560 570 580 590 600
KKSTSAPLLS LKNQINSDRM SDLMEIDFSQ ATNLEKQKFI KNNEIPKYIE
610 620 630 640 650
NVFPKAPRTD SSSLTLHATS QKDIFNGTKL NNTAITSEDG YLEMKPVGNG
660 670 680 690 700
YTPSSNCLPM KVEKLKLSDY QTAPPLTATA APVHDLNKIS TYNISAEKWR
710 720 730 740 750
EQPSRSEEKK SNSPLNDNTF SSKPTNVEST SKSHDVHSAN QIDCEKVCAQ
760 770 780 790 800
SSDKLNNHLA DKIVENNNLD IGGHEEKKLV HSISSEDYTQ IKDKSNDFTK
810 820 830 840 850
FNEAGYKILQ IKSDSSLISS KLYQKGIHKD NLERSQRLTE SVNTIPDNAT
860 870 880 890 900
ATAVSSSSLT KFNINSAKPA AAADSRSTGT DPSTPQNILQ IKDLNFPSRS
910 920 930 940 950
SSRISQPELH YASLDLPHCS GQNPAKYLKR GSRESPPVSA CPEDGNTYAK
960
IDFDQSDSSS SSSNIFNT
Length:968
Mass (Da):107,832
Last modified:November 1, 1999 - v1
Checksum:i04E9856F37F7A4CF
GO

Sequence cautioni

The sequence AAD55429 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29Y → C in strain: S108. 1 Publication1
Natural varianti165P → T in strain: FSP2, FSP15, FSP16, FSP19, FSP23, FSP29, RR8, RR11, RR17, RR18, RR35, RR52, S76, SL5_131, SL5_29, T28 and T39. 1 Publication1
Natural varianti334Missing in strain: S108. 1 Publication1
Natural varianti429A → V in strain: FSP19, RR33 and S108. 1 Publication1
Natural varianti452A → T in strain: RR33, S108, T28 and T39. 1 Publication1
Natural varianti664K → R in strain: FSP2, FSP3, FSP15, FSP19, FSP23, FSP25, FSP29, RR8, RR11, RR17, RR18, RR33, RR35, RR52, S76, S107, S108, T9, T15, T28, T39 and T41. 1 Publication1
Natural varianti675P → S in strain: S103. 1 Publication1
Natural varianti676L → I in strain: FSP3, FSP15, FSP19, FSP23, FSP25, RR8, RR11, RR17, RR33, RR35, RR52, S76, S97, S107, S108, T9, T28, T39 and T41. 1 Publication1
Natural varianti721S → G in strain: FSP2, FSP3, FSP15, FSP23, FSP25, FSP29, RR8, RR11, RR17, RR18, RR33, RR35, RR52, S108, T9, T15, T28, T39 and T41. 1 Publication1
Natural varianti722S → L in strain: FSP2, FSP3, FSP15, FSP16, FSP19, FSP23, FSP25, FSP29, RR8, RR11, RR17, RR18, RR33, RR35, RR52, S76, S97, S103, S107, S108, SL5_131, SL5_29, T9, T15, T28, T39 and T41. 1 Publication1
Natural varianti744C → S in strain: FSP3, FSP15, FSP16, FSP23, FSP25, FSP29, RR8, RR11, RR17, RR33, RR35, RR52, S108, T9, T15, T28, T39 and T41. 1 Publication1
Natural varianti758Missing in strain: FSP2, FSP3, FSP15, FSP16, FSP23, FSP25. FSP29, RR8, RR11, RR17, RR18, RR35, RR52, S97, S103, S108, SL5_131, SL5_29, T9, T15 and T41. 1 Publication1
Natural varianti808I → M in strain: RR8 and T41. 1 Publication1
Natural varianti851A → V in strain: FSP19. 1 Publication1
Natural varianti873A → T in strain: FSP16. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF154826 mRNA. Translation: AAD40880.1.
AF092046 mRNA. Translation: AAD43005.1.
GQ927177 Genomic DNA. Translation: ACY01718.1.
GQ927178 Genomic DNA. Translation: ACY01719.1.
GQ927179 Genomic DNA. Translation: ACY01720.1.
GQ927180 Genomic DNA. Translation: ACY01721.1.
GQ927181 Genomic DNA. Translation: ACY01722.1.
GQ927182 Genomic DNA. Translation: ACY01723.1.
GQ927183 Genomic DNA. Translation: ACY01724.1.
GQ927184 Genomic DNA. Translation: ACY01725.1.
GQ927185 Genomic DNA. Translation: ACY01726.1.
GQ927186 Genomic DNA. Translation: ACY01727.1.
GQ927187 Genomic DNA. Translation: ACY01728.1.
GQ927188 Genomic DNA. Translation: ACY01729.1.
GQ927189 Genomic DNA. Translation: ACY01730.1.
GQ927190 Genomic DNA. Translation: ACY01731.1.
GQ927191 Genomic DNA. Translation: ACY01732.1.
GQ927192 Genomic DNA. Translation: ACY01733.1.
GQ927193 Genomic DNA. Translation: ACY01734.1.
GQ927194 Genomic DNA. Translation: ACY01735.1.
GQ927195 Genomic DNA. Translation: ACY01736.1.
GQ927196 Genomic DNA. Translation: ACY01737.1.
GQ927197 Genomic DNA. Translation: ACY01738.1.
GQ927198 Genomic DNA. Translation: ACY01739.1.
GQ927199 Genomic DNA. Translation: ACY01740.1.
GQ927200 Genomic DNA. Translation: ACY01741.1.
GQ927201 Genomic DNA. Translation: ACY01742.1.
GQ927202 Genomic DNA. Translation: ACY01743.1.
GQ927203 Genomic DNA. Translation: ACY01744.1.
AE014134 Genomic DNA. Translation: AAF52882.1.
BT016137 mRNA. Translation: AAV37022.1.
AF181643 mRNA. Translation: AAD55429.1. Different initiation.
RefSeqiNP_001188772.1. NM_001201843.2.
NP_001260316.1. NM_001273387.1.
NP_723540.1. NM_164899.3.
UniGeneiDm.3017.

Genome annotation databases

EnsemblMetazoaiFBtr0080088; FBpp0079677; FBgn0024248.
FBtr0303904; FBpp0292907; FBgn0024248.
FBtr0333412; FBpp0305604; FBgn0024248.
GeneIDi64880.
KEGGidme:Dmel_CG5686.
UCSCiCG5686-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiIRS1_DROME
AccessioniPrimary (citable) accession number: Q9XTN2
Secondary accession number(s): D0UY43
, D0UY45, D0UY46, D0UY47, D0UY49, D0UY50, D0UY51, D0UY52, D0UY54, D0UY55, D0UY61, D0UY62, D0UY64, D0UY65, D0UY66, D0UY67, D0UY68, Q5U0V5, Q9U4G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: November 1, 1999
Last modified: December 20, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase