ID   3HIDH_CAEEL             Reviewed;         299 AA.
AC   Q9XTI0;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial;
DE            Short=HIBADH;
DE            EC=1.1.1.31;
GN   ORFNames=B0250.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY: 3-hydroxy-2-methylpropanoate + NAD(+) = 2-
CC       methyl-3-oxopropanoate + NADH.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the 3-hydroxyisobutyrate dehydrogenase
CC       family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; Z81453; CAB03798.1; -; Genomic_DNA.
DR   EMBL; AL031630; CAB03798.1; JOINED; Genomic_DNA.
DR   EMBL; AL031630; CAA21003.1; -; Genomic_DNA.
DR   EMBL; Z81453; CAA21003.1; JOINED; Genomic_DNA.
DR   PIR; T18682; T18682.
DR   RefSeq; NP_507943.1; -.
DR   UniGene; Cel.9442; -.
DR   Ensembl; B0250.5; Caenorhabditis elegans.
DR   GeneID; 180344; -.
DR   KEGG; cel:B0250.5; -.
DR   NMPDR; fig|6239.3.peg.22089; -.
DR   WormBase; WBGene00007122; B0250.5.
DR   WormPep; B0250.5; CE18481.
DR   OMA; Q9XTI0; PMARNLI.
DR   BRENDA; 1.1.1.31; 672.
DR   NextBio; 908956; -.
DR   ArrayExpress; Q9XTI0; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0004616; F:phosphogluconate dehydrogenase (decarboxyla...; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006098; P:pentose-phosphate shunt; IEA:InterPro.
DR   GO; GO:0006573; P:valine metabolic process; IEA:InterPro.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR015815; 3hydroxyacid_DH/Rdtase.
DR   InterPro; IPR006115; 6PGDH_NAD-bd.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR011548; IsoBut3OH_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR22981; 3hydroxy_acid_DH; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   TIGRFAMs; TIGR01692; HIBADH; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Mitochondrion; NAD; Oxidoreductase.
FT   CHAIN         1    299       Probable 3-hydroxyisobutyrate
FT                                dehydrogenase, mitochondrial.
FT                                /FTId=PRO_0000173055.
FT   NP_BIND       2     30       NAD (Potential).
FT   ACT_SITE    172    172       Probable.
SQ   SEQUENCE   299 AA;  31217 MW;  F30B423A597CABF0 CRC64;
     MSLTGFIGLG NMGGHMARNL IKNGKKLIVY DVNKAVVQEF KAEGCEVAAH PADIAAASKE
     IITVLPSSPH VKAVYQGEAG IFKTIQPGTL CMDSSTIDQI VSLEVAQAAA LLKAEYIDAP
     ISGGVTGAQQ ATLTFMVGAG NDATFKRAEA VLSLMGKNIV NLGAVGNGTA AKICNNMLLG
     IQMVAVAETM NLGISMGLDA KALAGIVNTS SGRCWSSDTY NPVPGVIENI PSCRGYAGGF
     GTTLMAKDLS LAQNASTNTQ APTPMGSLAH QIYRILARDP QYQAKDFGVV YQFLKKQNS
//