Reviewed, UniProtKB/Swiss-Prot Q9XTI0 (3HIDH_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
      Short name=HIBADH
    EC=1.1.1.31

Gene names

ORF Names:

B0250.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial		PRO_0000173055
Regions
	Nucleotide binding	2 – 30	29	NAD By similarity
Sites
	Active site	172	1	By similarity
	Binding site	96	1	NAD By similarity
	Binding site	247	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XTI0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F30B423A597CABF0	FASTA	299	31,217
	10 20 30 40 50 60 MSLTGFIGLG NMGGHMARNL IKNGKKLIVY DVNKAVVQEF KAEGCEVAAH PADIAAASKE 70 80 90 100 110 120 IITVLPSSPH VKAVYQGEAG IFKTIQPGTL CMDSSTIDQI VSLEVAQAAA LLKAEYIDAP 130 140 150 160 170 180 ISGGVTGAQQ ATLTFMVGAG NDATFKRAEA VLSLMGKNIV NLGAVGNGTA AKICNNMLLG 190 200 210 220 230 240 IQMVAVAETM NLGISMGLDA KALAGIVNTS SGRCWSSDTY NPVPGVIENI PSCRGYAGGF 250 260 270 280 290 GTTLMAKDLS LAQNASTNTQ APTPMGSLAH QIYRILARDP QYQAKDFGVV YQFLKKQNS

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81453, AL031630 Genomic DNA. Translation: CAB03798.1. AL031630, Z81453 Genomic DNA. Translation: CAA21003.1.
PIR	T18682.
RefSeq	NP_507943.1.
UniGene	Cel.9442
3D structure databases
SMR	Q9XTI0. Positions 4-298.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XTI0.
Genome annotation databases
Ensembl	B0250.5; B0250.5; B0250.5; Caenorhabditis elegans. [Genome view]
GeneID	180344.
KEGG	cel:B0250.5.
NMPDR	fig|6239.3.peg.22089.
UCSC	B0250.5. c. elegans.
Organism-specific databases
CTD	180344.
WormBase	WBGene00007122. B0250.5.
WormPep	B0250.5. CE18481. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04019.
HOGENOM	HBG729179.
InParanoid	Q9XTI0.
OMA	PMARNLI.
PhylomeDB	Q9XTI0.
Enzyme and pathway databases
BRENDA	1.1.1.31. 672.
Gene expression databases
ArrayExpress	Q9XTI0.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR008927. 6-PGluconate_DH_C-like. IPR006115. 6PGDH_NAD-bd. IPR013328. DH_multihelical. IPR011548. IsoBut3OH_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01692. HIBADH. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	908956.

Entry information

Entry name

3HIDH_CAEEL

Accession

Primary (citable) accession number: Q9XTI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project