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Reviewed, UniProtKB/Swiss-Prot Q9XTG7 (AKT2_CAEEL)

Last modified January 19, 2010. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase akt-2
    EC=2.7.11.1
Alternative name(s):
    Protein kinase B akt-2
      Short name=PKB akt-2
Gene names
Name: akt-2
ORF Names: F28H6.1
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Acts downstream of age-1 and pdk-1 in the daf-2/insulin receptor-like transduction pathway. Essential role in regulating developmental arrest at the dauer stage. Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their functions. Role in immune function and pathogen resistance. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Enzyme regulation

Phosphorylated and activated by pdk-1. Ref.8

Subunit structure

Interacts with pdk-1, sgk-1, akt-1 and daf-16. Part of a complex containing sgk-1, akt-1 and akt-2. Ref.8

Tissue specificity

Expressed in neurons, muscle cells of the pharynx, rectal gland cells, and spermatheca. Ref.1

Developmental stage

Expressed in late stage embryos and throughout life. Ref.1

Disruption phenotype

Defective egg-laying and increased resistance to pathogens. Simultaneous knockdown of akt-1 and akt-2 result in dauer formation and a weak extension to life span. Ref.6 Ref.8

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q9XTG7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: Q9XTG7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     470-483: EFTSMPVQLTPPRR → VRYVSILLKVSEAI
     484-528: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528Serine/threonine-protein kinase akt-2
PRO_0000085616

Regions

Domain12 – 115104PH
Domain180 – 437258Protein kinase
Domain438 – 51578AGC-kinase C-terminal
Nucleotide binding186 – 1949ATP By similarity

Sites

Active site3031Proton acceptor By similarity
Binding site2091ATP By similarity

Natural variations

Alternative sequence470 – 48314EFTSM…TPPRR → VRYVSILLKVSEAI in isoform b.
VSP_017047
Alternative sequence484 – 52845Missing in isoform b.
VSP_017048

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Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 9750E8F0AE4F6AE7

FASTA52861,058
        10         20         30         40         50         60 
MSTENAHLQK EDIVIESWLH KKGEHIRNWR PRYFILFRDG TLLGFRSKPK EDQPLPEPLN 

        70         80         90        100        110        120 
NFMIRDAATV CLDKPRPNMF IVRCLQWTTV IERTFYADSA DFRQMWIEAI QAVSSHNRLK 

       130        140        150        160        170        180 
ENAGNTSMQE EDTNGNPSGE SDVNMDATST RSDNDFESTV MNIDEPEEVP RKNTVTMDDF 

       190        200        210        220        230        240 
DFLKVLGQGT FGKVILCREK SSDKLYAIKI IRKEMVVDRS EVAHTLTENR VLYACVHPFL 

       250        260        270        280        290        300 
TLLKYSFQAQ YHICFVMEFA NGGELFTHLQ RCKTFSEART RFYGSEIILA LGYLHHRNIV 

       310        320        330        340        350        360 
YRDMKLENLL LDRDGHIKIT DFGLCKEEIK YGDKTSTFCG TPEYLAPEVI EDIDYDRSVD 

       370        380        390        400        410        420 
WWGVGVVMYE MMCGRLPFSA KENGKLFELI TTCDLKFPNR LSPEAVTLLS GLLERVPAKR 

       430        440        450        460        470        480 
LGAGPDDARE VSRAEFFKDV DWEATLRKEV EPPFKPNVMS ETDTSFFDRE FTSMPVQLTP 

       490        500        510        520 
PRRGEELPTV DEEEELQANF IQFASYYVSG SLERSYDTNR SADKYEIR

« Hide

Isoform b.

Checksum: 7977EE98BF40CD83
Show »

FASTA48355,757

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References

« Hide 'large scale' references
[1]"Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor."
Paradis S., Ruvkun G.
Genes Dev. 12:2488-2498(1998) [PubMed: 9716402] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans."
Paradis S., Ailion M., Toker A., Thomas J.H., Ruvkun G.
Genes Dev. 13:1438-1452(1999) [PubMed: 10364160] [Abstract]
Cited for: FUNCTION.
[4]"daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans."
Henderson S.T., Johnson T.E.
Curr. Biol. 11:1975-1980(2001) [PubMed: 11747825] [Abstract]
Cited for: FUNCTION.
[5]"Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling."
Lin K., Hsin H., Libina N., Kenyon C.
Nat. Genet. 28:139-145(2001) [PubMed: 11381260] [Abstract]
Cited for: FUNCTION.
[6]"The DAF-2 insulin-like signaling pathway independently regulates aging and immunity in C. elegans."
Evans E.A., Chen W.C., Tan M.-W.
Aging Cell 7:879-893(2008) [PubMed: 18782349] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans."
Tullet J.M., Hertweck M., An J.H., Baker J., Hwang J.Y., Liu S., Oliveira R.P., Baumeister R., Blackwell T.K.
Cell 132:1025-1038(2008) [PubMed: 18358814] [Abstract]
Cited for: FUNCTION.
[8]"C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span."
Hertweck M., Goebel C., Baumeister R.
Dev. Cell 6:577-588(2004) [PubMed: 15068796] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH PDK-1; SGK-1; AKT-1 AND DAF-16, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF072381 mRNA. Translation: AAC62468.1.
AL031621, Z92837 Genomic DNA. Translation: CAA20936.1.
Z92837, AL031621 Genomic DNA. Translation: CAB07403.1.
AL031621, Z92837 Genomic DNA. Translation: CAC70087.1.
Z92837, AL031621 Genomic DNA. Translation: CAD21654.1.
PIRT21523.
T43234.
RefSeqNP_001024612.1.
NP_510357.3.
UniGeneCel.18147

3D structure databases

SMRQ9XTG7. Positions 9-116, 175-476.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-26368N.
IntActQ9XTG7. 14 interactions.
STRINGQ9XTG7.

Proteomic databases

PRIDEQ9XTG7.

Genome annotation databases

EnsemblF28H6.1a; F28H6.1a; F28H6.1; Caenorhabditis elegans. [Genome view]
GeneID181524.
KEGGcel:F28H6.1.
UCSCF28H6.1b. c. elegans.

Organism-specific databases

CTD181524.
WormBaseWBGene00000103. akt-2.
WormPepF28H6.1a. CE18646. [WorfDB]
F28H6.1b. CE29298. [WorfDB]

Phylogenomic databases

eggNOGmeNOG06525.
HOGENOMHBG755340.
InParanoidQ9XTG7.
PhylomeDBQ9XTG7.

Enzyme and pathway databases

BRENDA2.7.11.1. 672.

Gene expression databases

ArrayExpressQ9XTG7.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_type.
IPR017892. Pkinase_C.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
IPR015744. Serine/threonine_Kinase_Rac.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
PANTHERPTHR22985:SF69. Akt. 1 hit.
PfamPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio914298.

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Entry information

Entry nameAKT2_CAEEL
AccessionPrimary (citable) accession number: Q9XTG7
Secondary accession number(s): O77145
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1999
Last modified: January 19, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents