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Protein

Serine/threonine-protein kinase akt-2

Gene

akt-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts downstream of age-1 and pdk-1 in the daf-2/insulin receptor-like transduction pathway. Essential role in regulating developmental arrest at the dauer stage. Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their functions. Role in immune function and pathogen resistance.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Phosphorylated and activated by pdk-1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei209 – 2091ATPPROSITE-ProRule annotation
Active sitei303 – 3031Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi186 – 1949ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: WormBase
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • innate immune response Source: UniProtKB-KW
  • insulin receptor signaling pathway Source: WormBase
  • peptidyl-serine phosphorylation Source: WormBase
  • peptidyl-threonine phosphorylation Source: WormBase
  • protein phosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-1257604. PIP3 activates AKT signaling.
R-CEL-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-CEL-165158. Activation of AKT2.
R-CEL-198323. AKT phosphorylates targets in the cytosol.
R-CEL-198693. AKT phosphorylates targets in the nucleus.
R-CEL-199418. Negative regulation of the PI3K/AKT network.
R-CEL-203615. eNOS activation.
R-CEL-211163. AKT-mediated inactivation of FOXO1A.
R-CEL-354192. Integrin alphaIIb beta3 signaling.
R-CEL-389357. CD28 dependent PI3K/Akt signaling.
R-CEL-389513. CTLA4 inhibitory signaling.
R-CEL-392451. G beta:gamma signalling through PI3Kgamma.
R-CEL-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
R-CEL-450604. KSRP (KHSRP) binds and destabilizes mRNA.
R-CEL-5218920. VEGFR2 mediated vascular permeability.
R-CEL-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
SignaLinkiQ9XTG7.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase akt-2 (EC:2.7.11.1)
Alternative name(s):
Protein kinase B akt-2
Short name:
PKB akt-2
Gene namesi
Name:akt-2
ORF Names:F28H6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF28H6.1a; CE18646; WBGene00000103; akt-2.
F28H6.1b; CE29298; WBGene00000103; akt-2.

Pathology & Biotechi

Disruption phenotypei

Defective egg-laying and increased resistance to pathogens. Simultaneous knockdown of akt-1 and akt-2 result in dauer formation and a weak extension to life span.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Serine/threonine-protein kinase akt-2PRO_0000085616Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9XTG7.
PaxDbiQ9XTG7.
PRIDEiQ9XTG7.

PTM databases

iPTMnetiQ9XTG7.

Expressioni

Tissue specificityi

Expressed in neurons, muscle cells of the pharynx, rectal gland cells, and spermatheca.1 Publication

Developmental stagei

Expressed in late stage embryos and throughout life.1 Publication

Interactioni

Subunit structurei

Interacts with pdk-1, sgk-1, akt-1 and daf-16. Part of a complex containing sgk-1, akt-1 and akt-2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
akt-1Q179412EBI-320656,EBI-1770718
daf-16O168503EBI-320656,EBI-324028
sgk-1Q2PJ683EBI-320656,EBI-1770776

Protein-protein interaction databases

BioGridi46423. 6 interactions.
DIPiDIP-26368N.
IntActiQ9XTG7. 8 interactions.
MINTiMINT-1058855.
STRINGi6239.F28H6.1a.

Structurei

3D structure databases

ProteinModelPortaliQ9XTG7.
SMRiQ9XTG7. Positions 13-528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 115104PHPROSITE-ProRule annotationAdd
BLAST
Domaini180 – 437258Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini438 – 51578AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126961.
HOGENOMiHOG000233033.
InParanoidiQ9XTG7.
KOiK04456.
OMAiVIIAKXS.
OrthoDBiEOG7Q5HCW.
PhylomeDBiQ9XTG7.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9XTG7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTENAHLQK EDIVIESWLH KKGEHIRNWR PRYFILFRDG TLLGFRSKPK
60 70 80 90 100
EDQPLPEPLN NFMIRDAATV CLDKPRPNMF IVRCLQWTTV IERTFYADSA
110 120 130 140 150
DFRQMWIEAI QAVSSHNRLK ENAGNTSMQE EDTNGNPSGE SDVNMDATST
160 170 180 190 200
RSDNDFESTV MNIDEPEEVP RKNTVTMDDF DFLKVLGQGT FGKVILCREK
210 220 230 240 250
SSDKLYAIKI IRKEMVVDRS EVAHTLTENR VLYACVHPFL TLLKYSFQAQ
260 270 280 290 300
YHICFVMEFA NGGELFTHLQ RCKTFSEART RFYGSEIILA LGYLHHRNIV
310 320 330 340 350
YRDMKLENLL LDRDGHIKIT DFGLCKEEIK YGDKTSTFCG TPEYLAPEVI
360 370 380 390 400
EDIDYDRSVD WWGVGVVMYE MMCGRLPFSA KENGKLFELI TTCDLKFPNR
410 420 430 440 450
LSPEAVTLLS GLLERVPAKR LGAGPDDARE VSRAEFFKDV DWEATLRKEV
460 470 480 490 500
EPPFKPNVMS ETDTSFFDRE FTSMPVQLTP PRRGEELPTV DEEEELQANF
510 520
IQFASYYVSG SLERSYDTNR SADKYEIR
Note: No experimental confirmation available.
Length:528
Mass (Da):61,058
Last modified:November 1, 1999 - v1
Checksum:i9750E8F0AE4F6AE7
GO
Isoform b (identifier: Q9XTG7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-483: EFTSMPVQLTPPRR → VRYVSILLKVSEAI
     484-528: Missing.

Show »
Length:483
Mass (Da):55,757
Checksum:i7977EE98BF40CD83
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei470 – 48314EFTSM…TPPRR → VRYVSILLKVSEAI in isoform b. 1 PublicationVSP_017047Add
BLAST
Alternative sequencei484 – 52845Missing in isoform b. 1 PublicationVSP_017048Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072381 mRNA. Translation: AAC62468.1.
AL031621, Z92837 Genomic DNA. Translation: CAA20936.1.
AL031621, Z92837 Genomic DNA. Translation: CAC70087.1.
PIRiT21523.
T43234.
RefSeqiNP_001024612.1. NM_001029441.3. [Q9XTG7-2]
NP_510357.3. NM_077956.5. [Q9XTG7-1]
UniGeneiCel.18147.

Genome annotation databases

EnsemblMetazoaiF28H6.1a; F28H6.1a; WBGene00000103. [Q9XTG7-1]
GeneIDi181524.
KEGGicel:CELE_F28H6.1.
UCSCiF28H6.1b. c. elegans. [Q9XTG7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072381 mRNA. Translation: AAC62468.1.
AL031621, Z92837 Genomic DNA. Translation: CAA20936.1.
AL031621, Z92837 Genomic DNA. Translation: CAC70087.1.
PIRiT21523.
T43234.
RefSeqiNP_001024612.1. NM_001029441.3. [Q9XTG7-2]
NP_510357.3. NM_077956.5. [Q9XTG7-1]
UniGeneiCel.18147.

3D structure databases

ProteinModelPortaliQ9XTG7.
SMRiQ9XTG7. Positions 13-528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi46423. 6 interactions.
DIPiDIP-26368N.
IntActiQ9XTG7. 8 interactions.
MINTiMINT-1058855.
STRINGi6239.F28H6.1a.

PTM databases

iPTMnetiQ9XTG7.

Proteomic databases

EPDiQ9XTG7.
PaxDbiQ9XTG7.
PRIDEiQ9XTG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF28H6.1a; F28H6.1a; WBGene00000103. [Q9XTG7-1]
GeneIDi181524.
KEGGicel:CELE_F28H6.1.
UCSCiF28H6.1b. c. elegans. [Q9XTG7-1]

Organism-specific databases

CTDi181524.
WormBaseiF28H6.1a; CE18646; WBGene00000103; akt-2.
F28H6.1b; CE29298; WBGene00000103; akt-2.

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126961.
HOGENOMiHOG000233033.
InParanoidiQ9XTG7.
KOiK04456.
OMAiVIIAKXS.
OrthoDBiEOG7Q5HCW.
PhylomeDBiQ9XTG7.

Enzyme and pathway databases

ReactomeiR-CEL-114604. GPVI-mediated activation cascade.
R-CEL-1257604. PIP3 activates AKT signaling.
R-CEL-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-CEL-165158. Activation of AKT2.
R-CEL-198323. AKT phosphorylates targets in the cytosol.
R-CEL-198693. AKT phosphorylates targets in the nucleus.
R-CEL-199418. Negative regulation of the PI3K/AKT network.
R-CEL-203615. eNOS activation.
R-CEL-211163. AKT-mediated inactivation of FOXO1A.
R-CEL-354192. Integrin alphaIIb beta3 signaling.
R-CEL-389357. CD28 dependent PI3K/Akt signaling.
R-CEL-389513. CTLA4 inhibitory signaling.
R-CEL-392451. G beta:gamma signalling through PI3Kgamma.
R-CEL-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
R-CEL-450604. KSRP (KHSRP) binds and destabilizes mRNA.
R-CEL-5218920. VEGFR2 mediated vascular permeability.
R-CEL-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
SignaLinkiQ9XTG7.

Miscellaneous databases

PROiQ9XTG7.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor."
    Paradis S., Ruvkun G.
    Genes Dev. 12:2488-2498(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans."
    Paradis S., Ailion M., Toker A., Thomas J.H., Ruvkun G.
    Genes Dev. 13:1438-1452(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans."
    Henderson S.T., Johnson T.E.
    Curr. Biol. 11:1975-1980(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling."
    Lin K., Hsin H., Libina N., Kenyon C.
    Nat. Genet. 28:139-145(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The DAF-2 insulin-like signaling pathway independently regulates aging and immunity in C. elegans."
    Evans E.A., Chen W.C., Tan M.-W.
    Aging Cell 7:879-893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans."
    Tullet J.M., Hertweck M., An J.H., Baker J., Hwang J.Y., Liu S., Oliveira R.P., Baumeister R., Blackwell T.K.
    Cell 132:1025-1038(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span."
    Hertweck M., Goebel C., Baumeister R.
    Dev. Cell 6:577-588(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH PDK-1; SGK-1; AKT-1 AND DAF-16, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAKT2_CAEEL
AccessioniPrimary (citable) accession number: Q9XTG7
Secondary accession number(s): O77145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.